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The map label for this gene is pcm3 [H]

Identifier: 182682580

GI number: 182682580

Start: 2323518

End: 2324174

Strand: Direct

Name: pcm3 [H]

Synonym: XfasM23_2068

Alternate gene names: 182682580

Gene position: 2323518-2324174 (Clockwise)

Preceding gene: 182682579

Following gene: 182682581

Centisome position: 91.63

GC content: 53.88

Gene sequence:

>657_bases
ATGACCATTGATTTTTCCATAGCCCGCGAAAAAATGGTCGAACAGCAGATCCGTCCATGGGATGTGGTGGATCTGCACGT
ACTTGATGTACTGGCACACATCCCACGCGAGGCGTTCGTCCCTGAACCATACCGTACCCTAGCCTACGCAGACCTTGAAA
TCCCCTTACCCGGCGGACAAACAATGATGAAACCCGTCATTGAGGGGCGCCTACTTCAAGCCCTGATGTTGTCACCAGAA
GAGGACGTATTGGAAATCGGCACCGGCAGTGGTTTCCTCACTGCCTGCTTGGCTTCACTGGGACACGAAGTAGTCAGCCT
AGAGATCAATTCCGCACTGGCCGCATCGGCACGTACCCACCTAGATACCATTGGCCTAGGCAGTAACGTACACATTGAGC
AGGCCGACGCTTTCACTTGGCAACCCGAACGTCAGTTCAGCGTCATTTGCTTGACTGGTGCAGTGAGCACGCTGCCACTG
CAATTTCTGCAATGGCTACACCCCAACGGCCGTATGTTCGCTATACACGGCCACTCCCCAGCGATGCAAGCTGTTCTGGT
GCGTGGAGATGCCTCTACCCCAAACATCACATCCCTGTTTGAGACCGATCTGGCTTACCTCAAAGGTGGCGAGCCAGCCC
TCCAATTTCATTTTTGA

Upstream 100 bases:

>100_bases
ATAGTGCAAAGCAGACGCATAGACCACACAGATCAAGAGCCATGACCCCACATCAAAGCACGGCTAAAATGCGTGTCCCA
CCGCGTCGAAAATTGACACC

Downstream 100 bases:

>100_bases
TACCTGACTCAAGGATAACGCTATGATCCGCCGCTGCCTCGCCTTGGCCGTGACCACCGCCCTATTTCCCGTCGTTTCAC
AAGCGACAAACCTATTACAA

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase 3; Protein L-isoaspartyl methyltransferase 3; Protein-beta-aspartate methyltransferase 3; PIMT 3 [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MTIDFSIAREKMVEQQIRPWDVVDLHVLDVLAHIPREAFVPEPYRTLAYADLEIPLPGGQTMMKPVIEGRLLQALMLSPE
EDVLEIGTGSGFLTACLASLGHEVVSLEINSALAASARTHLDTIGLGSNVHIEQADAFTWQPERQFSVICLTGAVSTLPL
QFLQWLHPNGRMFAIHGHSPAMQAVLVRGDASTPNITSLFETDLAYLKGGEPALQFHF

Sequences:

>Translated_218_residues
MTIDFSIAREKMVEQQIRPWDVVDLHVLDVLAHIPREAFVPEPYRTLAYADLEIPLPGGQTMMKPVIEGRLLQALMLSPE
EDVLEIGTGSGFLTACLASLGHEVVSLEINSALAASARTHLDTIGLGSNVHIEQADAFTWQPERQFSVICLTGAVSTLPL
QFLQWLHPNGRMFAIHGHSPAMQAVLVRGDASTPNITSLFETDLAYLKGGEPALQFHF
>Mature_217_residues
TIDFSIAREKMVEQQIRPWDVVDLHVLDVLAHIPREAFVPEPYRTLAYADLEIPLPGGQTMMKPVIEGRLLQALMLSPEE
DVLEIGTGSGFLTACLASLGHEVVSLEINSALAASARTHLDTIGLGSNVHIEQADAFTWQPERQFSVICLTGAVSTLPLQ
FLQWLHPNGRMFAIHGHSPAMQAVLVRGDASTPNITSLFETDLAYLKGGEPALQFHF

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=156, Percent_Identity=36.5384615384615, Blast_Score=95, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 23922; Mature: 23791

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDFSIAREKMVEQQIRPWDVVDLHVLDVLAHIPREAFVPEPYRTLAYADLEIPLPGGQ
CEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHCCCCCHHEEEEEEEEEECCCCH
TMMKPVIEGRLLQALMLSPEEDVLEIGTGSGFLTACLASLGHEVVSLEINSALAASARTH
HHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCEEEEEEECHHHHHHHHHH
LDTIGLGSNVHIEQADAFTWQPERQFSVICLTGAVSTLPLQFLQWLHPNGRMFAIHGHSP
HHEECCCCCEEEECCCCEEECCCCCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCC
AMQAVLVRGDASTPNITSLFETDLAYLKGGEPALQFHF
CEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure 
TIDFSIAREKMVEQQIRPWDVVDLHVLDVLAHIPREAFVPEPYRTLAYADLEIPLPGGQ
EECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHCCCCCHHEEEEEEEEEECCCCH
TMMKPVIEGRLLQALMLSPEEDVLEIGTGSGFLTACLASLGHEVVSLEINSALAASARTH
HHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCEEEEEEECHHHHHHHHHH
LDTIGLGSNVHIEQADAFTWQPERQFSVICLTGAVSTLPLQFLQWLHPNGRMFAIHGHSP
HHEECCCCCEEEECCCCEEECCCCCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCC
AMQAVLVRGDASTPNITSLFETDLAYLKGGEPALQFHF
CEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA