Click here to switch to the map view.

The map label for this gene is pilB [H]

Identifier: 182682546

GI number: 182682546

Start: 2275635

End: 2277368

Strand: Direct

Name: pilB [H]

Synonym: XfasM23_2034

Alternate gene names: 182682546

Gene position: 2275635-2277368 (Clockwise)

Preceding gene: 182682545

Following gene: 182682549

Centisome position: 89.74

GC content: 51.96

Gene sequence:

>1734_bases
ATGAACACACCTCATTCAGCCAATCTTGTCGGCATCACCGGTATTGCCCGTCGCCTGGTCCAGGATGGCAGCATTGAGGA
AGCCACTGCACGCACTGCTCAAAATCAGGCAATGGCCGCAAAAGTGCCTTTACCACAGTGGTTGATCGATAAAAAACTAG
TGAGTGCAGCAAAATTGGCCGCGGCCAATGCACTTGAGTTCGGGATGCCGCTACTGGATGTGTCCGCATTCGACCACAAT
CACAATGCAATGAACTTGGTCGACGAAGAATTACTGCAAAAGCATCGAGTGTTGCCATTATTTAAACGCGGTAATCGACT
GTTCGTCGGGATCAGCAATCCAACTCAAACACAGGCCCTGGACGACATCAAATTCCACACCAATCTGGTCGTGGAACCGA
TTCTGGTCGACGAAGACCAGATACGGCGCACACTTGAACAATGGCAATCTAGGAGCGGCGCGCTCAGCTCCGCATTGGAT
GAAGAGGATAACGGCATTGAAGGGCTGGATATCGGCGGCGGAGACGACGACGGGGCCAGTGGCGATACAGGGATTGATGC
CAAGAGCGATGACACACCTGTCGTCAAATTCGTCAACAAAGTGCTGATTGATGCCATCCGACGCGGCGCATCAGACATCC
ACTTTGAACCATACGAAGAAGATTACCGCGTCCGTTTCCGGATTGATGGTCTGCTGAAGGCTGTGGCAAAAGCACCGAAC
AAGTTGAATCAGCGTATCGCCGCACGCTTGAAAGTCATGTCGCAACTGGATATCGCGGAAAAACGCGTCCCTCAAGACGG
ACGTATCAAGCTTAATCTGTCGAAAACCAAGCAAGTCGATTTCCGCGTCAGCACCTTGCCAACGCTGTTCGGCGAGAAAG
TGGTACTGCGTATCTTGGATGGCAGCGCGGCAAAGCTGGGTATCGACAAACTCGGTTACGAAGCTGACCAACAAACGCTT
TTTATGGAAGCAATCCACAAACCCTATGGAATGGTACTGGTCACTGGTCCGACCGGTTCGGGCAAGACAGTCTCACTTTA
CACTGCGTTAGGCATCCTCAATGACGAAACACGCAATATCTCCACAGCCGAAGATCCTGTAGAAATCCGGCTGCCGGGTG
TCAATCAGGTACAACAAAACAACAAGCGCGGTATGACGTTTGCCGCAGCATTACGTTCGTTTCTGCGCCAAGACCCGGAT
GTGATCATGGTCGGCGAAATCCGTGATCTGGAAACCGCTGAAATCGCCATCAAAGCCGCACAGACCGGCCACATGGTGCT
CTCCACCCTACATACCAACGATGCACCGCAAACCATCGCACGCCTCATGAACATGGGGATTGCACCATACAACATCACTT
CATCCGTGACCTTAGTGATTGCACAGCGATTGGCACGGAGATTGTGCAACAAGTGCAAGCGCCCGGCTCAATTGCCGACA
AACGCATTACTGGCCGAAGGATTCACTCAAGAACAAATTGATGCGGGGCTGGAATTATACGAGCCTGTCGGTTGCGACGA
ATGCACTGAAGGCTACAAAGGCCGCACAGGTATTTATCAAGTGATGCCAATTACCGAAGGGATTTCCTCGATCATTTTAG
CCGGCGGTAATGCCTTACAAATCGCCGAAGAGGCACAGCGCATCGGCATCAACGACCTACGCCAATCGGCGCTACTCAAA
GTCGCTCAGGGCATCACCGGGCTGGCTGAAATTAATCGAGTCACCAAAGATTAG

Upstream 100 bases:

>100_bases
CGACACAAGCGCCTTCGATTGACGTACCCCTCCCGATAACTGCTCATTGGAAAAGCCAACGGGCACCATACTTTAAAGCA
GAGTAACCAGAGAACTTTTC

Downstream 100 bases:

>100_bases
GCATCAACAACACCTGTTAATGACTGAATCAAAGCACGTCATGACACCATGCTGAGAAGAAGCAAGGCAGGCTATGTTGC
CTGCTTCCTCTCATCACTCC

Product: type IV-A pilus assembly ATPase PilB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MNTPHSANLVGITGIARRLVQDGSIEEATARTAQNQAMAAKVPLPQWLIDKKLVSAAKLAAANALEFGMPLLDVSAFDHN
HNAMNLVDEELLQKHRVLPLFKRGNRLFVGISNPTQTQALDDIKFHTNLVVEPILVDEDQIRRTLEQWQSRSGALSSALD
EEDNGIEGLDIGGGDDDGASGDTGIDAKSDDTPVVKFVNKVLIDAIRRGASDIHFEPYEEDYRVRFRIDGLLKAVAKAPN
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQVDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQTL
FMEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
VIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNKCKRPAQLPT
NALLAEGFTQEQIDAGLELYEPVGCDECTEGYKGRTGIYQVMPITEGISSIILAGGNALQIAEEAQRIGINDLRQSALLK
VAQGITGLAEINRVTKD

Sequences:

>Translated_577_residues
MNTPHSANLVGITGIARRLVQDGSIEEATARTAQNQAMAAKVPLPQWLIDKKLVSAAKLAAANALEFGMPLLDVSAFDHN
HNAMNLVDEELLQKHRVLPLFKRGNRLFVGISNPTQTQALDDIKFHTNLVVEPILVDEDQIRRTLEQWQSRSGALSSALD
EEDNGIEGLDIGGGDDDGASGDTGIDAKSDDTPVVKFVNKVLIDAIRRGASDIHFEPYEEDYRVRFRIDGLLKAVAKAPN
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQVDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQTL
FMEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
VIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNKCKRPAQLPT
NALLAEGFTQEQIDAGLELYEPVGCDECTEGYKGRTGIYQVMPITEGISSIILAGGNALQIAEEAQRIGINDLRQSALLK
VAQGITGLAEINRVTKD
>Mature_577_residues
MNTPHSANLVGITGIARRLVQDGSIEEATARTAQNQAMAAKVPLPQWLIDKKLVSAAKLAAANALEFGMPLLDVSAFDHN
HNAMNLVDEELLQKHRVLPLFKRGNRLFVGISNPTQTQALDDIKFHTNLVVEPILVDEDQIRRTLEQWQSRSGALSSALD
EEDNGIEGLDIGGGDDDGASGDTGIDAKSDDTPVVKFVNKVLIDAIRRGASDIHFEPYEEDYRVRFRIDGLLKAVAKAPN
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQVDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQTL
FMEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
VIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNKCKRPAQLPT
NALLAEGFTQEQIDAGLELYEPVGCDECTEGYKGRTGIYQVMPITEGISSIILAGGNALQIAEEAQRIGINDLRQSALLK
VAQGITGLAEINRVTKD

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=394, Percent_Identity=44.9238578680203, Blast_Score=337, Evalue=2e-93,
Organism=Escherichia coli, GI1789723, Length=383, Percent_Identity=45.4308093994778, Blast_Score=331, Evalue=8e-92,
Organism=Escherichia coli, GI87082188, Length=251, Percent_Identity=32.2709163346614, Blast_Score=116, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62948; Mature: 62948

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPHSANLVGITGIARRLVQDGSIEEATARTAQNQAMAAKVPLPQWLIDKKLVSAAKLA
CCCCCCCCEEEHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHH
AANALEFGMPLLDVSAFDHNHNAMNLVDEELLQKHRVLPLFKRGNRLFVGISNPTQTQAL
HHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCHHEECCCEEEEEECCCCHHHHH
DDIKFHTNLVVEPILVDEDQIRRTLEQWQSRSGALSSALDEEDNGIEGLDIGGGDDDGAS
HHHHHHCCEEEEEEEECHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEECCCCCCCCCC
GDTGIDAKSDDTPVVKFVNKVLIDAIRRGASDIHFEPYEEDYRVRFRIDGLLKAVAKAPN
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCHH
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQVDFRVSTLPTLFGEKVVLRILD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEHHHHHHHHCCHHEEEEEC
GSAAKLGIDKLGYEADQQTLFMEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNI
CCCHHCCHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEHHHCCCCCCCC
STAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDVIMVGEIRDLETAEIAIKAA
CCCCCCEEEECCCCHHHHHCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEC
QTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNKCKRPAQLPT
CCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
NALLAEGFTQEQIDAGLELYEPVGCDECTEGYKGRTGIYQVMPITEGISSIILAGGNALQ
HHHHHCCCCHHHHHCCCHHCCCCCCHHHHCCCCCCCCCEEEEEHHHHHHHHHHCCCCHHH
IAEEAQRIGINDLRQSALLKVAQGITGLAEINRVTKD
HHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MNTPHSANLVGITGIARRLVQDGSIEEATARTAQNQAMAAKVPLPQWLIDKKLVSAAKLA
CCCCCCCCEEEHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHH
AANALEFGMPLLDVSAFDHNHNAMNLVDEELLQKHRVLPLFKRGNRLFVGISNPTQTQAL
HHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCHHEECCCEEEEEECCCCHHHHH
DDIKFHTNLVVEPILVDEDQIRRTLEQWQSRSGALSSALDEEDNGIEGLDIGGGDDDGAS
HHHHHHCCEEEEEEEECHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEECCCCCCCCCC
GDTGIDAKSDDTPVVKFVNKVLIDAIRRGASDIHFEPYEEDYRVRFRIDGLLKAVAKAPN
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCHH
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQVDFRVSTLPTLFGEKVVLRILD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEHHHHHHHHCCHHEEEEEC
GSAAKLGIDKLGYEADQQTLFMEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNI
CCCHHCCHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEHHHCCCCCCCC
STAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDVIMVGEIRDLETAEIAIKAA
CCCCCCEEEECCCCHHHHHCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEC
QTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNKCKRPAQLPT
CCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
NALLAEGFTQEQIDAGLELYEPVGCDECTEGYKGRTGIYQVMPITEGISSIILAGGNALQ
HHHHHCCCCHHHHHCCCHHCCCCCCHHHHCCCCCCCCCEEEEEHHHHHHHHHHCCCCHHH
IAEEAQRIGINDLRQSALLKVAQGITGLAEINRVTKD
HHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]