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The map label for this gene is pheS

Identifier: 182682529

GI number: 182682529

Start: 2258658

End: 2259659

Strand: Reverse

Name: pheS

Synonym: XfasM23_2016

Alternate gene names: 182682529

Gene position: 2259659-2258658 (Counterclockwise)

Preceding gene: 182682530

Following gene: 182682528

Centisome position: 89.11

GC content: 50.5

Gene sequence:

>1002_bases
ATGGTAATCAATGACATTGAATCCCTGACAGCCCAAGCATTGGCTGAGGTGGCGGCTGCGCAGAATTTAGATCATTTGGA
ATTTTTGCGTGTAGCTCTTCTTGGTAAAAATGGCAGCATCACCTTGCAGCTCAAGCAACTGGGTAAATTGCCAGTTGAGC
AGCGTAAGGCGATTGGCGAAAAGCTCAACCGCGTCCGGGATCTTATCTCGGACGCATTAATGGATCGCAAAGCTGCGTTG
GAAAGTGCGGCGTTGAGCAAGCGCCTCATTGATGAGCGCGTTGATGTTACCCTCCCAGGGCGCCGAGGTGAACGAGCAGG
CTTGCATCCAGTCACCCGTACTTTGGAGCGTATTACCGAGATTTTTGCCCGGCTGGGTTACGAGCTGGTTGAAGGTCCGG
AAATTGAGGACGACTGGCATAATTTTGAGGCGCTGAACTTCCCATTGCACCATCCAGCGCGTGCTATGCATGACACCTTT
TATTTTGGTGACGGCCGTTTGCTACGGACTCATACTTCTGGTGTACAGGTGCGTTACATGAGTGACCACCGGCCGCCGCT
GCGTATGATCGCTGCTGGCAAGGTTTATCGCAGCGATAGTGATCAGACGCACTCGCCAATGTTCCATCAGATAGAAGGAT
TATTGCTTGACAAGCATGCCACTTTTGTTGACTTGAAAGGGACCTTGTCCGAGTTCTTGAGAGCGTTTTTTGAGCGTGAT
TTTGAGGTGCGTTTTCGCCCAAGTTATTTCCCGTTTGTCGAACCGGGTGCGGAAGTTGATATTGCTTGGCAACAGTCCGA
CAGCAGCGTTCGATGGCTGGAGGTGCTGGGTTGCGGCATGGTACATCCGAACGTGCTGAAGAATGTTGGCATTGATTCGG
AATGTTATACAGGCTTTGCATTTGGACTAGGTGTGGAACGTTTTGCGATGCTGCGTTACGGCGTCGACGACTTACGTGCT
TTTTTCGAGAATGATGTGAGATTTTTACGCCAGTTTTCTTGA

Upstream 100 bases:

>100_bases
TTACGTTTTAGAGTTGAATGAGTATGAGCAACATCACACCCAGCGTTGCGCGTAATGCAAACCGTCTAGCATACGACGAC
AGGATGGGTAGCTAAAGCGT

Downstream 100 bases:

>100_bases
AACTGCTGTCTTACCCTGATCTTGCTACATTCTGATGGTGCTGAGCCTGTTTTGCTCTTTTCCCGTTTCCAGATCCGACT
CTCATGAAATTCAGCGAAAA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MVINDIESLTAQALAEVAAAQNLDHLEFLRVALLGKNGSITLQLKQLGKLPVEQRKAIGEKLNRVRDLISDALMDRKAAL
ESAALSKRLIDERVDVTLPGRRGERAGLHPVTRTLERITEIFARLGYELVEGPEIEDDWHNFEALNFPLHHPARAMHDTF
YFGDGRLLRTHTSGVQVRYMSDHRPPLRMIAAGKVYRSDSDQTHSPMFHQIEGLLLDKHATFVDLKGTLSEFLRAFFERD
FEVRFRPSYFPFVEPGAEVDIAWQQSDSSVRWLEVLGCGMVHPNVLKNVGIDSECYTGFAFGLGVERFAMLRYGVDDLRA
FFENDVRFLRQFS

Sequences:

>Translated_333_residues
MVINDIESLTAQALAEVAAAQNLDHLEFLRVALLGKNGSITLQLKQLGKLPVEQRKAIGEKLNRVRDLISDALMDRKAAL
ESAALSKRLIDERVDVTLPGRRGERAGLHPVTRTLERITEIFARLGYELVEGPEIEDDWHNFEALNFPLHHPARAMHDTF
YFGDGRLLRTHTSGVQVRYMSDHRPPLRMIAAGKVYRSDSDQTHSPMFHQIEGLLLDKHATFVDLKGTLSEFLRAFFERD
FEVRFRPSYFPFVEPGAEVDIAWQQSDSSVRWLEVLGCGMVHPNVLKNVGIDSECYTGFAFGLGVERFAMLRYGVDDLRA
FFENDVRFLRQFS
>Mature_333_residues
MVINDIESLTAQALAEVAAAQNLDHLEFLRVALLGKNGSITLQLKQLGKLPVEQRKAIGEKLNRVRDLISDALMDRKAAL
ESAALSKRLIDERVDVTLPGRRGERAGLHPVTRTLERITEIFARLGYELVEGPEIEDDWHNFEALNFPLHHPARAMHDTF
YFGDGRLLRTHTSGVQVRYMSDHRPPLRMIAAGKVYRSDSDQTHSPMFHQIEGLLLDKHATFVDLKGTLSEFLRAFFERD
FEVRFRPSYFPFVEPGAEVDIAWQQSDSSVRWLEVLGCGMVHPNVLKNVGIDSECYTGFAFGLGVERFAMLRYGVDDLRA
FFENDVRFLRQFS

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=286, Percent_Identity=31.4685314685315, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI4758340, Length=267, Percent_Identity=29.2134831460674, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI1788007, Length=330, Percent_Identity=59.0909090909091, Blast_Score=417, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI32566635, Length=243, Percent_Identity=32.5102880658436, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI32563657, Length=264, Percent_Identity=26.8939393939394, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17508957, Length=264, Percent_Identity=26.8939393939394, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6321087, Length=372, Percent_Identity=26.6129032258064, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325304, Length=95, Percent_Identity=38.9473684210526, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI18858079, Length=267, Percent_Identity=28.8389513108614, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI17137424, Length=242, Percent_Identity=29.3388429752066, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_XYLF2 (B2I9P4)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830689.1
- ProteinModelPortal:   B2I9P4
- SMR:   B2I9P4
- GeneID:   6203372
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_2016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 37931; Mature: 37931

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVINDIESLTAQALAEVAAAQNLDHLEFLRVALLGKNGSITLQLKQLGKLPVEQRKAIGE
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEHHCCCCCHHHHHHHHH
KLNRVRDLISDALMDRKAALESAALSKRLIDERVDVTLPGRRGERAGLHPVTRTLERITE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHH
IFARLGYELVEGPEIEDDWHNFEALNFPLHHPARAMHDTFYFGDGRLLRTHTSGVQVRYM
HHHHHCHHHHCCCCCCCCCCCCEEECCCCCCCHHHHHCEEEECCCEEEEEECCCEEEEEE
SDHRPPLRMIAAGKVYRSDSDQTHSPMFHQIEGLLLDKHATFVDLKGTLSEFLRAFFERD
CCCCCCHHHHCCCHHCCCCCCCCCCCHHHHHHHHEEECCCCEEECHHHHHHHHHHHHCCC
FEVRFRPSYFPFVEPGAEVDIAWQQSDSSVRWLEVLGCGMVHPNVLKNVGIDSECYTGFA
CEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHH
FGLGVERFAMLRYGVDDLRAFFENDVRFLRQFS
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVINDIESLTAQALAEVAAAQNLDHLEFLRVALLGKNGSITLQLKQLGKLPVEQRKAIGE
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEHHCCCCCHHHHHHHHH
KLNRVRDLISDALMDRKAALESAALSKRLIDERVDVTLPGRRGERAGLHPVTRTLERITE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHH
IFARLGYELVEGPEIEDDWHNFEALNFPLHHPARAMHDTFYFGDGRLLRTHTSGVQVRYM
HHHHHCHHHHCCCCCCCCCCCCEEECCCCCCCHHHHHCEEEECCCEEEEEECCCEEEEEE
SDHRPPLRMIAAGKVYRSDSDQTHSPMFHQIEGLLLDKHATFVDLKGTLSEFLRAFFERD
CCCCCCHHHHCCCHHCCCCCCCCCCCHHHHHHHHEEECCCCEEECHHHHHHHHHHHHCCC
FEVRFRPSYFPFVEPGAEVDIAWQQSDSSVRWLEVLGCGMVHPNVLKNVGIDSECYTGFA
CEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHH
FGLGVERFAMLRYGVDDLRAFFENDVRFLRQFS
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA