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The map label for this gene is pheT

Identifier: 182682528

GI number: 182682528

Start: 2256196

End: 2258574

Strand: Reverse

Name: pheT

Synonym: XfasM23_2015

Alternate gene names: 182682528

Gene position: 2258574-2256196 (Counterclockwise)

Preceding gene: 182682529

Following gene: 182682527

Centisome position: 89.07

GC content: 54.1

Gene sequence:

>2379_bases
ATGAAATTCAGCGAAAATTGGTTGCGCAATCATGCTCCTATCCAAGCCAATCGTGACGTGCTTGTAGCGACCCTGACCGC
GATAGGCCTTGAAGTTGAAAACGTGGCAGTGTTAGGTGAAGCTTTGGACTTGATCTTAGTGGCGCGCATCGTCAATGTCG
TGCCTCATCCCGAATCTGATCTCTTACAAATCTGTCAAGTCGATGTTGCTCAAGACACCCTTTTACAGATTGTCTGCGGC
GCTTCAAACGTGCGTCCTGGCTTGGTGGTGCCATTGGCGTTGCTTGGTGCCAAGATCGGTGCACTGACGATCAAGTCCAC
CACACTGCGTGGGATCGAATCCAATGGCATGTTGTGCTCAGCCAAAGAACTTGGCTTGGATACAGAGGCGTCCGGTCTGA
TGGAACTCCCGGAAGATGCACCGATTGGTACACCATTAGCCGATTATCTTGCTTTGCCCGATGCTAGTATTGAAATCAAA
TTGACCCCGAACCGTGCTGATTGCTTCAGCGTGCGCGGCATTGCTTTCGACGTGGCTGCGGCCTGTGCCAGTGAGGTGAC
CCCGTTTCATATTGACGAGATCCCCGCTGTTTCTGCGCGTACGCTGCCTGTTGAATTACATGCTGGTGCCAATGCCCCAC
GCTATTGTGGCTGTGTGATTGAGGGTATCGACCCTGCTGCCCCTACTCCGGTGTGGATGGCCGAGCGCCTGCGTCGCAGC
GGTATTCGTCCGGTGTCATTGCTGGTTGATATCACCCAGTACGTCATGTTGGAACTGGGGCAGCCGATGCATGCTTTCGA
CGTGGATACCCTACGTGGCCCCATTGGTGTACGTCTCTCCCGCAATGATGAAGCCTTGAAGCTTCTTGATGGACGTACAG
TTGTGCTTGATAACGATTTCCTAGTTGTGACTGATGCCGATCAACCTATTGCACTGGCTGGTTTGATCGGCGGTTGGGAA
ACACGCATAACAGATACCACTATCAACGTCTTTCTTGAGGCTGCTCACTTTGCACCGGCTGCGATCATGGGACGTGGCCG
TAAACTTGGCCTGCATACCGATGCCAGCCACCGCTTCGAGCGCGGCGTAGATCCAGCCTTACCCCCGCAAGCGATCGCTT
TTGCGACCCGCCTCATACTGGAACTTGCTGGTGGCAAGCCAGGGTCATTGATTCACGTTCAATTGCCCGAGTATTTACCG
GCACCGGCTTCGATCTTGCTGCGTCGCACACGTATCGCGCGCCTCCTTGGCATTGTCATTGATGATGCCGAGGTAGAGCG
TATTTTGCAGGCCCTGGGGATGCAGGTAACGACTCAAGCTGAAGGTTGGCGCGTTGTTGCACCAAGCCGCCGTTTTGATA
TTGCGATTGAAGAAGATCTGATCGAAGAGCTGGTACGTATCCGTGGTTACGAACATCTACCAACCGCCTTGCCGGTTGGA
GCGAGTCATATCGCAATGCCCAGTGAAACCCGACTCGATATGACCAGCGTGCGTCGACAATTGATTGCACGTGAATTACA
AGAAACCATCAACTACGCCTTCATTGATGCTGAGCTACTGCGCCGTTGGCAATTGAATACTGGTCAAGTCATGTTGATGA
ATCCCCTGAGTGCGGAGTTGGCAGTGATGCGACCACGCTTGTTACCAGGTTTGGTGGCAGCCTTGGGGCGCAACATCGCC
CGTCAGCTGGAACGCGTGCGCTTGTTTGAACTGGGCAATGTATTTACTGCATCTGATGAGGCAGGAGCAGCACCCCTTGA
AACCCGACACGTTGCTGCTGCCGTATGTGGCGATGCGTTCGCCTTGCAGTGGGGTGAACAGGTGCGTAAGGTCGATTTCT
ACGATCTGAAAGGGGACTTGGAATCCCTAGCTGCGGCCAGCGGTGCAGTCTTGACCTTCCATTCCTCAGCACAGCCATGG
GGCCATCCCGGCCGCTCAGCCGACGTTTGGTGTGATGACATGTGTATTGGCTGGATTGGACAATTGCATCCGGCACTTAC
CCAAACACTGGAAATCAACGTGGATGTGATCGCCTTCGAACTGGCTTTGGAACCGCTAGTACGCCGCGCACTTCCGCGTG
CTCATGCACTCTCACGTTTCCCATTCGTGCGCCGCGACTTGGCCTGTGTCGTGCCCGAACACGTTACTTGGAGCGAACTG
GCAATCACCGTGCGCGATGTCATTGGACCGTTACTTCGCGACGTGAAACTGTTTGATCGCTACGTCGGTAAGGGTATCGA
ACCAGGCTTTAAAAGCCTGGCTATTGGCTTGATTTTGCAGGATGACACGCGCACTCTGATCGACCGTGACGTTGACGACA
TCATGACGAAGGTTGTCATGGCGATCCAGCAACGACACGACGTGCGGATCCGGTCTTGA

Upstream 100 bases:

>100_bases
TACGCCAGTTTTCTTGAAACTGCTGTCTTACCCTGATCTTGCTACATTCTGATGGTGCTGAGCCTGTTTTGCTCTTTTCC
CGTTTCCAGATCCGACTCTC

Downstream 100 bases:

>100_bases
TGTAAATAAAGGGGGTACACCAGCATGGCATTGACAAAAGCAGAGATGGTAGAACGTTTATTCGATGAAGTCGGCTTGAA
TAAACGCGAAGCAAAAGAAT

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 792; Mature: 792

Protein sequence:

>792_residues
MKFSENWLRNHAPIQANRDVLVATLTAIGLEVENVAVLGEALDLILVARIVNVVPHPESDLLQICQVDVAQDTLLQIVCG
ASNVRPGLVVPLALLGAKIGALTIKSTTLRGIESNGMLCSAKELGLDTEASGLMELPEDAPIGTPLADYLALPDASIEIK
LTPNRADCFSVRGIAFDVAAACASEVTPFHIDEIPAVSARTLPVELHAGANAPRYCGCVIEGIDPAAPTPVWMAERLRRS
GIRPVSLLVDITQYVMLELGQPMHAFDVDTLRGPIGVRLSRNDEALKLLDGRTVVLDNDFLVVTDADQPIALAGLIGGWE
TRITDTTINVFLEAAHFAPAAIMGRGRKLGLHTDASHRFERGVDPALPPQAIAFATRLILELAGGKPGSLIHVQLPEYLP
APASILLRRTRIARLLGIVIDDAEVERILQALGMQVTTQAEGWRVVAPSRRFDIAIEEDLIEELVRIRGYEHLPTALPVG
ASHIAMPSETRLDMTSVRRQLIARELQETINYAFIDAELLRRWQLNTGQVMLMNPLSAELAVMRPRLLPGLVAALGRNIA
RQLERVRLFELGNVFTASDEAGAAPLETRHVAAAVCGDAFALQWGEQVRKVDFYDLKGDLESLAAASGAVLTFHSSAQPW
GHPGRSADVWCDDMCIGWIGQLHPALTQTLEINVDVIAFELALEPLVRRALPRAHALSRFPFVRRDLACVVPEHVTWSEL
AITVRDVIGPLLRDVKLFDRYVGKGIEPGFKSLAIGLILQDDTRTLIDRDVDDIMTKVVMAIQQRHDVRIRS

Sequences:

>Translated_792_residues
MKFSENWLRNHAPIQANRDVLVATLTAIGLEVENVAVLGEALDLILVARIVNVVPHPESDLLQICQVDVAQDTLLQIVCG
ASNVRPGLVVPLALLGAKIGALTIKSTTLRGIESNGMLCSAKELGLDTEASGLMELPEDAPIGTPLADYLALPDASIEIK
LTPNRADCFSVRGIAFDVAAACASEVTPFHIDEIPAVSARTLPVELHAGANAPRYCGCVIEGIDPAAPTPVWMAERLRRS
GIRPVSLLVDITQYVMLELGQPMHAFDVDTLRGPIGVRLSRNDEALKLLDGRTVVLDNDFLVVTDADQPIALAGLIGGWE
TRITDTTINVFLEAAHFAPAAIMGRGRKLGLHTDASHRFERGVDPALPPQAIAFATRLILELAGGKPGSLIHVQLPEYLP
APASILLRRTRIARLLGIVIDDAEVERILQALGMQVTTQAEGWRVVAPSRRFDIAIEEDLIEELVRIRGYEHLPTALPVG
ASHIAMPSETRLDMTSVRRQLIARELQETINYAFIDAELLRRWQLNTGQVMLMNPLSAELAVMRPRLLPGLVAALGRNIA
RQLERVRLFELGNVFTASDEAGAAPLETRHVAAAVCGDAFALQWGEQVRKVDFYDLKGDLESLAAASGAVLTFHSSAQPW
GHPGRSADVWCDDMCIGWIGQLHPALTQTLEINVDVIAFELALEPLVRRALPRAHALSRFPFVRRDLACVVPEHVTWSEL
AITVRDVIGPLLRDVKLFDRYVGKGIEPGFKSLAIGLILQDDTRTLIDRDVDDIMTKVVMAIQQRHDVRIRS
>Mature_792_residues
MKFSENWLRNHAPIQANRDVLVATLTAIGLEVENVAVLGEALDLILVARIVNVVPHPESDLLQICQVDVAQDTLLQIVCG
ASNVRPGLVVPLALLGAKIGALTIKSTTLRGIESNGMLCSAKELGLDTEASGLMELPEDAPIGTPLADYLALPDASIEIK
LTPNRADCFSVRGIAFDVAAACASEVTPFHIDEIPAVSARTLPVELHAGANAPRYCGCVIEGIDPAAPTPVWMAERLRRS
GIRPVSLLVDITQYVMLELGQPMHAFDVDTLRGPIGVRLSRNDEALKLLDGRTVVLDNDFLVVTDADQPIALAGLIGGWE
TRITDTTINVFLEAAHFAPAAIMGRGRKLGLHTDASHRFERGVDPALPPQAIAFATRLILELAGGKPGSLIHVQLPEYLP
APASILLRRTRIARLLGIVIDDAEVERILQALGMQVTTQAEGWRVVAPSRRFDIAIEEDLIEELVRIRGYEHLPTALPVG
ASHIAMPSETRLDMTSVRRQLIARELQETINYAFIDAELLRRWQLNTGQVMLMNPLSAELAVMRPRLLPGLVAALGRNIA
RQLERVRLFELGNVFTASDEAGAAPLETRHVAAAVCGDAFALQWGEQVRKVDFYDLKGDLESLAAASGAVLTFHSSAQPW
GHPGRSADVWCDDMCIGWIGQLHPALTQTLEINVDVIAFELALEPLVRRALPRAHALSRFPFVRRDLACVVPEHVTWSEL
AITVRDVIGPLLRDVKLFDRYVGKGIEPGFKSLAIGLILQDDTRTLIDRDVDDIMTKVVMAIQQRHDVRIRS

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=795, Percent_Identity=42.7672955974843, Blast_Score=607, Evalue=1e-175,
Organism=Caenorhabditis elegans, GI17533403, Length=286, Percent_Identity=25.1748251748252, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21355211, Length=267, Percent_Identity=26.2172284644195, Blast_Score=77, Evalue=6e-14,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 86425; Mature: 86425

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSENWLRNHAPIQANRDVLVATLTAIGLEVENVAVLGEALDLILVARIVNVVPHPESD
CCCCHHHHHCCCCCCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LLQICQVDVAQDTLLQIVCGASNVRPGLVVPLALLGAKIGALTIKSTTLRGIESNGMLCS
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEECCEECCCCCCCCEEE
AKELGLDTEASGLMELPEDAPIGTPLADYLALPDASIEIKLTPNRADCFSVRGIAFDVAA
HHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCEEECCHHHHHHH
ACASEVTPFHIDEIPAVSARTLPVELHAGANAPRYCGCVIEGIDPAAPTPVWMAERLRRS
HHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEECEEEECCCCCCCCHHHHHHHHHHC
GIRPVSLLVDITQYVMLELGQPMHAFDVDTLRGPIGVRLSRNDEALKLLDGRTVVLDNDF
CCCHHHHHHHHHHHHHHHCCCCCHHCCHHHHCCCCEEEECCCCCEEEEECCCEEEEECCE
LVVTDADQPIALAGLIGGWETRITDTTINVFLEAAHFAPAAIMGRGRKLGLHTDASHRFE
EEEECCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHCCCHHHHCCCCEEECCCCCHHHHH
RGVDPALPPQAIAFATRLILELAGGKPGSLIHVQLPEYLPAPASILLRRTRIARLLGIVI
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHC
DDAEVERILQALGMQVTTQAEGWRVVAPSRRFDIAIEEDLIEELVRIRGYEHLPTALPVG
CCHHHHHHHHHCCCEEEECCCCEEEECCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCC
ASHIAMPSETRLDMTSVRRQLIARELQETINYAFIDAELLRRWQLNTGQVMLMNPLSAEL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEEHHHHHHHCCCCCCEEEECCCCHHH
AVMRPRLLPGLVAALGRNIARQLERVRLFELGNVFTASDEAGAAPLETRHVAAAVCGDAF
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHCCHH
ALQWGEQVRKVDFYDLKGDLESLAAASGAVLTFHSSAQPWGHPGRSADVWCDDMCIGWIG
HHHHHHHHHHCEEEECCCCHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
QLHPALTQTLEINVDVIAFELALEPLVRRALPRAHALSRFPFVRRDLACVVPEHVTWSEL
HHHHHHHHEEEECEEEEEHHHHHHHHHHHHCCHHHHHHHCCHHHHCHHHCCCCCCCHHHH
AITVRDVIGPLLRDVKLFDRYVGKGIEPGFKSLAIGLILQDDTRTLIDRDVDDIMTKVVM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEEEECCCHHHHHCCHHHHHHHHHH
AIQQRHDVRIRS
HHHHHCCCCCCC
>Mature Secondary Structure
MKFSENWLRNHAPIQANRDVLVATLTAIGLEVENVAVLGEALDLILVARIVNVVPHPESD
CCCCHHHHHCCCCCCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LLQICQVDVAQDTLLQIVCGASNVRPGLVVPLALLGAKIGALTIKSTTLRGIESNGMLCS
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEECCEECCCCCCCCEEE
AKELGLDTEASGLMELPEDAPIGTPLADYLALPDASIEIKLTPNRADCFSVRGIAFDVAA
HHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCEEECCHHHHHHH
ACASEVTPFHIDEIPAVSARTLPVELHAGANAPRYCGCVIEGIDPAAPTPVWMAERLRRS
HHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEECEEEECCCCCCCCHHHHHHHHHHC
GIRPVSLLVDITQYVMLELGQPMHAFDVDTLRGPIGVRLSRNDEALKLLDGRTVVLDNDF
CCCHHHHHHHHHHHHHHHCCCCCHHCCHHHHCCCCEEEECCCCCEEEEECCCEEEEECCE
LVVTDADQPIALAGLIGGWETRITDTTINVFLEAAHFAPAAIMGRGRKLGLHTDASHRFE
EEEECCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHCCCHHHHCCCCEEECCCCCHHHHH
RGVDPALPPQAIAFATRLILELAGGKPGSLIHVQLPEYLPAPASILLRRTRIARLLGIVI
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHC
DDAEVERILQALGMQVTTQAEGWRVVAPSRRFDIAIEEDLIEELVRIRGYEHLPTALPVG
CCHHHHHHHHHCCCEEEECCCCEEEECCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCC
ASHIAMPSETRLDMTSVRRQLIARELQETINYAFIDAELLRRWQLNTGQVMLMNPLSAEL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEEHHHHHHHCCCCCCEEEECCCCHHH
AVMRPRLLPGLVAALGRNIARQLERVRLFELGNVFTASDEAGAAPLETRHVAAAVCGDAF
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHCCHH
ALQWGEQVRKVDFYDLKGDLESLAAASGAVLTFHSSAQPWGHPGRSADVWCDDMCIGWIG
HHHHHHHHHHCEEEECCCCHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
QLHPALTQTLEINVDVIAFELALEPLVRRALPRAHALSRFPFVRRDLACVVPEHVTWSEL
HHHHHHHHEEEECEEEEEHHHHHHHHHHHHCCHHHHHHHCCHHHHCHHHCCCCCCCHHHH
AITVRDVIGPLLRDVKLFDRYVGKGIEPGFKSLAIGLILQDDTRTLIDRDVDDIMTKVVM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEEEECCCHHHHHCCHHHHHHHHHH
AIQQRHDVRIRS
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]