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The map label for this gene is murF [H]

Identifier: 182682485

GI number: 182682485

Start: 2212422

End: 2213828

Strand: Reverse

Name: murF [H]

Synonym: XfasM23_1972

Alternate gene names: 182682485

Gene position: 2213828-2212422 (Counterclockwise)

Preceding gene: 182682486

Following gene: 182682484

Centisome position: 87.31

GC content: 56.15

Gene sequence:

>1407_bases
ATGAGATCTATGCCACTTTCGTTAATTGCGTACTGGACCGGTGGTTATCTGCACGGTGAGGATGTCAGGGTGTGTGCAAT
TGGTCATGACACCCGTGCGCTGGATGTTGGCGTGTTGTATGTGGCGTTGCGAGGCCAGCGTTTCGATGCGCATCTATTCT
GCGCCGAAGCGGTGGCACGTGGTGCGGCGGGGGTATTGGTTGATCACGTCTTGCCAGAGTTGGATGTTCCTCAGGTCGTT
GTGGCCGATACTGAGCGCGCGCTTGCGGCGATTGCTTCAGCCTTGCAGCGTGACCGGACATCTTTTGTGTTGGCATTGAC
TGGGTCCAATGGAAAAACCAGTGTTAAGGCAATGCTTGTATCCATTTTGGAGCATGCTGCGGCACAGAGTAGTACGGTGG
TCTATGCGAGTTCTGGTAACTGCAACAATGAGATTGGCCTGCCGTTGGCAGTGATTGCCGCGCCCGATAATGCCGATTTT
GCGGTGTATGAGATGGGTGCGGGCAAGTTGGGCGACATCGGTTATTTGACTGACATCGTTACGCCGGATGTGGCGTTGGT
TAATAACATCGCACCGGCGCACTTGGAGCGCATGGGGAGCTTGCTTGGTGTGGCGCGTACCAAAGGTGAGATCTATGCCG
CGTTGCGGCCTGGTGGTACTGCTGTGATCAATATGGATGATGCGTTTGCTTTGTGGTTTGAGCAGCAGTGTGTTCCGGCT
GGTTGTGCTGTGTTGCGGTTCGGTTTGCAGGCAACTGCTGACGTTACTGCGTATAGCCTGCAATTCAGTGAGGACAGTGT
GCGTTTTGTACTTGTGACGCCACAGGGGGAGGTGCCGGTGTGCTTGGCGCTGTCTGGGCGTCACAATGTGTGTAATGCGC
TGGCAGCGGCAGCAATGGCGTTGGTTGCTGGTATTCCTTTGTCGCAGATTGCGGCTGGTCTTGGTTTGGCTCGGTCGGTG
GCCGGTCGCCAAGTTGCGCATGCTTTACCCGGTGGTGCGGTATTGATTGATGATAGTTACAACGCCAATCCTGGGTCGCT
GGATGTGGCAATCGATACGTTGACGGCTGCTTCCGGCCCAGGATGGTTGGTGCTTGGCGATATGCTCGAGCTTGGTTTGG
AGGGGGTGGCGCTGCATGCCGCTGCTGGACGTCGTGCACGTCAAGCCAAAGTAGCCCGTTTATATGCACTGGGTGAGTTG
AGTGCAGTTGCAGCGCGGGAGTTTGGTGAGGGTGGACATGTGTTTGCCGATCATGATGCGTTGGCTGCGGTGTTGTGGGA
TGACTTGATGCATGTACCAGGTGGGGTGCCGCCGCGGGTGCTTGTGAAGGGGTCGCGGGGTAGTGCGATGGACAAAATTG
TGGGCGCTCTGTTGTCGAGTGCGGAGGGCGTAGCGCATGTTGTTTGA

Upstream 100 bases:

>100_bases
ACGAGCGATATCAGGAGGTTGCTGGGGTGCGTCATCCGTTCGATGACACTGAGGTTGCTATGCGTGTGCTGGCAGCAATG
TCGGCCCAGGAGACTGTCCG

Downstream 100 bases:

>100_bases
GTTTGCCCGTTGGTTGCAGCAATTTGAAAGTTTGTTTGGGTTGTTCAATTACCTGACGTTCCGCAGCATTTTGGCGGCAT
TGACTGCATTGTTTTTGTCG

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MRSMPLSLIAYWTGGYLHGEDVRVCAIGHDTRALDVGVLYVALRGQRFDAHLFCAEAVARGAAGVLVDHVLPELDVPQVV
VADTERALAAIASALQRDRTSFVLALTGSNGKTSVKAMLVSILEHAAAQSSTVVYASSGNCNNEIGLPLAVIAAPDNADF
AVYEMGAGKLGDIGYLTDIVTPDVALVNNIAPAHLERMGSLLGVARTKGEIYAALRPGGTAVINMDDAFALWFEQQCVPA
GCAVLRFGLQATADVTAYSLQFSEDSVRFVLVTPQGEVPVCLALSGRHNVCNALAAAAMALVAGIPLSQIAAGLGLARSV
AGRQVAHALPGGAVLIDDSYNANPGSLDVAIDTLTAASGPGWLVLGDMLELGLEGVALHAAAGRRARQAKVARLYALGEL
SAVAAREFGEGGHVFADHDALAAVLWDDLMHVPGGVPPRVLVKGSRGSAMDKIVGALLSSAEGVAHVV

Sequences:

>Translated_468_residues
MRSMPLSLIAYWTGGYLHGEDVRVCAIGHDTRALDVGVLYVALRGQRFDAHLFCAEAVARGAAGVLVDHVLPELDVPQVV
VADTERALAAIASALQRDRTSFVLALTGSNGKTSVKAMLVSILEHAAAQSSTVVYASSGNCNNEIGLPLAVIAAPDNADF
AVYEMGAGKLGDIGYLTDIVTPDVALVNNIAPAHLERMGSLLGVARTKGEIYAALRPGGTAVINMDDAFALWFEQQCVPA
GCAVLRFGLQATADVTAYSLQFSEDSVRFVLVTPQGEVPVCLALSGRHNVCNALAAAAMALVAGIPLSQIAAGLGLARSV
AGRQVAHALPGGAVLIDDSYNANPGSLDVAIDTLTAASGPGWLVLGDMLELGLEGVALHAAAGRRARQAKVARLYALGEL
SAVAAREFGEGGHVFADHDALAAVLWDDLMHVPGGVPPRVLVKGSRGSAMDKIVGALLSSAEGVAHVV
>Mature_468_residues
MRSMPLSLIAYWTGGYLHGEDVRVCAIGHDTRALDVGVLYVALRGQRFDAHLFCAEAVARGAAGVLVDHVLPELDVPQVV
VADTERALAAIASALQRDRTSFVLALTGSNGKTSVKAMLVSILEHAAAQSSTVVYASSGNCNNEIGLPLAVIAAPDNADF
AVYEMGAGKLGDIGYLTDIVTPDVALVNNIAPAHLERMGSLLGVARTKGEIYAALRPGGTAVINMDDAFALWFEQQCVPA
GCAVLRFGLQATADVTAYSLQFSEDSVRFVLVTPQGEVPVCLALSGRHNVCNALAAAAMALVAGIPLSQIAAGLGLARSV
AGRQVAHALPGGAVLIDDSYNANPGSLDVAIDTLTAASGPGWLVLGDMLELGLEGVALHAAAGRRARQAKVARLYALGEL
SAVAAREFGEGGHVFADHDALAAVLWDDLMHVPGGVPPRVLVKGSRGSAMDKIVGALLSSAEGVAHVV

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=458, Percent_Identity=41.4847161572052, Blast_Score=268, Evalue=5e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48271; Mature: 48271

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSMPLSLIAYWTGGYLHGEDVRVCAIGHDTRALDVGVLYVALRGQRFDAHLFCAEAVAR
CCCCCEEEEEEECCCEEECCCEEEEEECCCCCEEHHEEEEEEEECCCCCHHHHHHHHHHC
GAAGVLVDHVLPELDVPQVVVADTERALAAIASALQRDRTSFVLALTGSNGKTSVKAMLV
CCHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHH
SILEHAAAQSSTVVYASSGNCNNEIGLPLAVIAAPDNADFAVYEMGAGKLGDIGYLTDIV
HHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCHHHHHHC
TPDVALVNNIAPAHLERMGSLLGVARTKGEIYAALRPGGTAVINMDDAFALWFEQQCVPA
CCCHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCEEEEECCCCCCH
GCAVLRFGLQATADVTAYSLQFSEDSVRFVLVTPQGEVPVCLALSGRHNVCNALAAAAMA
HHHHHHHCCCCCCCEEEEEEEECCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHH
LVAGIPLSQIAAGLGLARSVAGRQVAHALPGGAVLIDDSYNANPGSLDVAIDTLTAASGP
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEEEECCCCC
GWLVLGDMLELGLEGVALHAAAGRRARQAKVARLYALGELSAVAAREFGEGGHVFADHDA
CEEEEHHHHHCCCCCEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHH
LAAVLWDDLMHVPGGVPPRVLVKGSRGSAMDKIVGALLSSAEGVAHVV
HHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEECC
>Mature Secondary Structure
MRSMPLSLIAYWTGGYLHGEDVRVCAIGHDTRALDVGVLYVALRGQRFDAHLFCAEAVAR
CCCCCEEEEEEECCCEEECCCEEEEEECCCCCEEHHEEEEEEEECCCCCHHHHHHHHHHC
GAAGVLVDHVLPELDVPQVVVADTERALAAIASALQRDRTSFVLALTGSNGKTSVKAMLV
CCHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHH
SILEHAAAQSSTVVYASSGNCNNEIGLPLAVIAAPDNADFAVYEMGAGKLGDIGYLTDIV
HHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCHHHHHHC
TPDVALVNNIAPAHLERMGSLLGVARTKGEIYAALRPGGTAVINMDDAFALWFEQQCVPA
CCCHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCEEEEECCCCCCH
GCAVLRFGLQATADVTAYSLQFSEDSVRFVLVTPQGEVPVCLALSGRHNVCNALAAAAMA
HHHHHHHCCCCCCCEEEEEEEECCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHH
LVAGIPLSQIAAGLGLARSVAGRQVAHALPGGAVLIDDSYNANPGSLDVAIDTLTAASGP
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEEEECCCCC
GWLVLGDMLELGLEGVALHAAAGRRARQAKVARLYALGELSAVAAREFGEGGHVFADHDA
CEEEEHHHHHCCCCCEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHH
LAAVLWDDLMHVPGGVPPRVLVKGSRGSAMDKIVGALLSSAEGVAHVV
HHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]