Click here to switch to the map view.

The map label for this gene is murE

Identifier: 182682486

GI number: 182682486

Start: 2213825

End: 2215300

Strand: Reverse

Name: murE

Synonym: XfasM23_1973

Alternate gene names: 182682486

Gene position: 2215300-2213825 (Counterclockwise)

Preceding gene: 182682487

Following gene: 182682485

Centisome position: 87.36

GC content: 56.3

Gene sequence:

>1476_bases
ATGCCATTGGCGCAGCTATTGCCCGATATTCCGCAAGCGCGTGATGTCGTGATCTCTGGTTTGGTGATGGATAGCCGTGA
GGTGCAGCCGGGTGATGCGTTTGTCGCGGTTGCAGGGTTCGGCGTTCACGGGTTGTGTTTTATTGAGGATGCGTGTGCCA
GAGGTGCTGTTGCAATTTTGTTTGAGCCGCCCGCACCGCAGGGGGTGTCAGTGCCGGATGGTGCGATTGCAGTGCATGGT
TTACGTGCGCGTTTGGGTGCGATGGCGGATCGTTTCCATGGCCATCCGTCGCAGGCGATGACCATGGTGGGGGTCACTGG
CACCAATGGTAAGACGTCCACTGTGCAGTTGTTGGCCCAGGCTTGGCATCGTCTGGGTGTGCGCAGTGCGACCTGTGGAA
CGCTCGGTGTGGGTTTGTACGATCAGCTGGTACCGACGGGTTTCACGACGCCGCTGGTATTGCAATTGCATCAGTGTCTG
GGACAACTGCGTGATGATGGTGCGCAGGCGGTGGCGATGGAAGTCAGTTCCCACGCTCTTGACCAGGGACGCGTGGATGG
CGTGCATTATGACGTTGCGGTGTTCACCAATCTGACGCGCGATCATCTTGATTATCACGGTGACATGGAGCACTACGGTG
AGGCTAAGGCTCGCTTGTTCGCGCATCAGGATGTGCAGGCGGCGGTGATCAATGTGGATGATCTTTTCGGTTTGCGTCTG
TTACATGGTTTGGCCAATGGGGTGCGGCGGGTTGGAGTCAGCGTGTGTGGTCACACGGATGCAGATGTGATGGCGCAGCA
TCTGAGTCTGAATCTTCAGGGTATCGGGTTTGATCTTGTCATCGGTGCGGATCGTGCGCCGGTGCGTTCACCTCTGATGG
GACGTTTCAACGTTGATAATTTGCTTACGGTAGCAGGTGTTCTTTATGCGCTGGATTACGCGCTGTCTGAGATTGCTGCG
GTGCTGTCGACGTTGCGGCCGATTCATGGCCGGATGAATCGTTTGGGTGGTCAGGATGGGCAGCCTGTGGTGGTGGTTGA
TTACGCGCATACCCCTGATGCGCTGGGGCAGGTGTTGTCCAGTTTGAGTAGCCACGTATGCGGTCGTTTGATTTGTGTGT
TTGGCTGTGGCGGTGAGCGCGATAGGGGTAAACGTTCACAGATGGCTGTTATTGCCGAGAGTAACGCCGATGTTGTGTTG
GTGACCGATGATAATCCGCGTGGTGAGGATGGTGATGGCATTGTTGCTGACATTCTGGCCGGTTTTGTGCGTCCCAACCT
CGTAAAAGTGCAGCGTGACCGTTCTGCTGCGATTGCGGCAGCCATTGGTATAGCCAGTGCAGGGGATGTTGTGTTGATTG
CTGGCAAAGGTCACGAGCGATATCAGGAGGTTGCTGGGGTGCGTCATCCGTTCGATGACACTGAGGTTGCTATGCGTGTG
CTGGCAGCAATGTCGGCCCAGGAGACTGTCCGATGA

Upstream 100 bases:

>100_bases
CATTAGTACCCGATGCTGCCGATGAGGTCGCCGCTGGTATTCCTACGACGGTGACACCATCGCCGTCTGCGATTCATAGA
GATCGCCAATGAGGCGTTCT

Downstream 100 bases:

>100_bases
GATCTATGCCACTTTCGTTAATTGCGTACTGGACCGGTGGTTATCTGCACGGTGAGGATGTCAGGGTGTGTGCAATTGGT
CATGACACCCGTGCGCTGGA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MPLAQLLPDIPQARDVVISGLVMDSREVQPGDAFVAVAGFGVHGLCFIEDACARGAVAILFEPPAPQGVSVPDGAIAVHG
LRARLGAMADRFHGHPSQAMTMVGVTGTNGKTSTVQLLAQAWHRLGVRSATCGTLGVGLYDQLVPTGFTTPLVLQLHQCL
GQLRDDGAQAVAMEVSSHALDQGRVDGVHYDVAVFTNLTRDHLDYHGDMEHYGEAKARLFAHQDVQAAVINVDDLFGLRL
LHGLANGVRRVGVSVCGHTDADVMAQHLSLNLQGIGFDLVIGADRAPVRSPLMGRFNVDNLLTVAGVLYALDYALSEIAA
VLSTLRPIHGRMNRLGGQDGQPVVVVDYAHTPDALGQVLSSLSSHVCGRLICVFGCGGERDRGKRSQMAVIAESNADVVL
VTDDNPRGEDGDGIVADILAGFVRPNLVKVQRDRSAAIAAAIGIASAGDVVLIAGKGHERYQEVAGVRHPFDDTEVAMRV
LAAMSAQETVR

Sequences:

>Translated_491_residues
MPLAQLLPDIPQARDVVISGLVMDSREVQPGDAFVAVAGFGVHGLCFIEDACARGAVAILFEPPAPQGVSVPDGAIAVHG
LRARLGAMADRFHGHPSQAMTMVGVTGTNGKTSTVQLLAQAWHRLGVRSATCGTLGVGLYDQLVPTGFTTPLVLQLHQCL
GQLRDDGAQAVAMEVSSHALDQGRVDGVHYDVAVFTNLTRDHLDYHGDMEHYGEAKARLFAHQDVQAAVINVDDLFGLRL
LHGLANGVRRVGVSVCGHTDADVMAQHLSLNLQGIGFDLVIGADRAPVRSPLMGRFNVDNLLTVAGVLYALDYALSEIAA
VLSTLRPIHGRMNRLGGQDGQPVVVVDYAHTPDALGQVLSSLSSHVCGRLICVFGCGGERDRGKRSQMAVIAESNADVVL
VTDDNPRGEDGDGIVADILAGFVRPNLVKVQRDRSAAIAAAIGIASAGDVVLIAGKGHERYQEVAGVRHPFDDTEVAMRV
LAAMSAQETVR
>Mature_490_residues
PLAQLLPDIPQARDVVISGLVMDSREVQPGDAFVAVAGFGVHGLCFIEDACARGAVAILFEPPAPQGVSVPDGAIAVHGL
RARLGAMADRFHGHPSQAMTMVGVTGTNGKTSTVQLLAQAWHRLGVRSATCGTLGVGLYDQLVPTGFTTPLVLQLHQCLG
QLRDDGAQAVAMEVSSHALDQGRVDGVHYDVAVFTNLTRDHLDYHGDMEHYGEAKARLFAHQDVQAAVINVDDLFGLRLL
HGLANGVRRVGVSVCGHTDADVMAQHLSLNLQGIGFDLVIGADRAPVRSPLMGRFNVDNLLTVAGVLYALDYALSEIAAV
LSTLRPIHGRMNRLGGQDGQPVVVVDYAHTPDALGQVLSSLSSHVCGRLICVFGCGGERDRGKRSQMAVIAESNADVVLV
TDDNPRGEDGDGIVADILAGFVRPNLVKVQRDRSAAIAAAIGIASAGDVVLIAGKGHERYQEVAGVRHPFDDTEVAMRVL
AAMSAQETVR

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=494, Percent_Identity=42.914979757085, Blast_Score=306, Evalue=2e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 51865; Mature: 51734

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLAQLLPDIPQARDVVISGLVMDSREVQPGDAFVAVAGFGVHGLCFIEDACARGAVAIL
CCHHHHCCCCCCHHHHHHHHEEECCCCCCCCCEEEEEECCCCCEEEEEEHHHCCCEEEEE
FEPPAPQGVSVPDGAIAVHGLRARLGAMADRFHGHPSQAMTMVGVTGTNGKTSTVQLLAQ
ECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHH
AWHRLGVRSATCGTLGVGLYDQLVPTGFTTPLVLQLHQCLGQLRDDGAQAVAMEVSSHAL
HHHHCCCCCCCCCHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHEEEHHHHHHHH
DQGRVDGVHYDVAVFTNLTRDHLDYHGDMEHYGEAKARLFAHQDVQAAVINVDDLFGLRL
CCCCCCCEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHH
LHGLANGVRRVGVSVCGHTDADVMAQHLSLNLQGIGFDLVIGADRAPVRSPLMGRFNVDN
HHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEECCEEEEECCCCCCCCCCCCCCCCHHH
LLTVAGVLYALDYALSEIAAVLSTLRPIHGRMNRLGGQDGQPVVVVDYAHTPDALGQVLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHH
SLSSHVCGRLICVFGCGGERDRGKRSQMAVIAESNADVVLVTDDNPRGEDGDGIVADILA
HHHHHHHHHEEEEEECCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHH
GFVRPNLVKVQRDRSAAIAAAIGIASAGDVVLIAGKGHERYQEVAGVRHPFDDTEVAMRV
HHHCCCEEEEECCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCCHHHHHHHH
LAAMSAQETVR
HHHHHHHHHCC
>Mature Secondary Structure 
PLAQLLPDIPQARDVVISGLVMDSREVQPGDAFVAVAGFGVHGLCFIEDACARGAVAIL
CHHHHCCCCCCHHHHHHHHEEECCCCCCCCCEEEEEECCCCCEEEEEEHHHCCCEEEEE
FEPPAPQGVSVPDGAIAVHGLRARLGAMADRFHGHPSQAMTMVGVTGTNGKTSTVQLLAQ
ECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHH
AWHRLGVRSATCGTLGVGLYDQLVPTGFTTPLVLQLHQCLGQLRDDGAQAVAMEVSSHAL
HHHHCCCCCCCCCHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHEEEHHHHHHHH
DQGRVDGVHYDVAVFTNLTRDHLDYHGDMEHYGEAKARLFAHQDVQAAVINVDDLFGLRL
CCCCCCCEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHH
LHGLANGVRRVGVSVCGHTDADVMAQHLSLNLQGIGFDLVIGADRAPVRSPLMGRFNVDN
HHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEECCEEEEECCCCCCCCCCCCCCCCHHH
LLTVAGVLYALDYALSEIAAVLSTLRPIHGRMNRLGGQDGQPVVVVDYAHTPDALGQVLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHH
SLSSHVCGRLICVFGCGGERDRGKRSQMAVIAESNADVVLVTDDNPRGEDGDGIVADILA
HHHHHHHHHEEEEEECCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHH
GFVRPNLVKVQRDRSAAIAAAIGIASAGDVVLIAGKGHERYQEVAGVRHPFDDTEVAMRV
HHHCCCEEEEECCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCCHHHHHHHH
LAAMSAQETVR
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]