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The map label for this gene is glyA

Identifier: 182682365

GI number: 182682365

Start: 2052122

End: 2053375

Strand: Reverse

Name: glyA

Synonym: XfasM23_1851

Alternate gene names: 182682365

Gene position: 2053375-2052122 (Counterclockwise)

Preceding gene: 182682368

Following gene: 182682364

Centisome position: 80.98

GC content: 53.35

Gene sequence:

>1254_bases
ATGTTCCCGCGTGATGCTCGTCTTGATATGTACGATCCCGAGTTGGCTAAGGCCATCGCTGCTGAAGTGAGGCGTCAGGA
GGATCACGTTGAGTTGATTGCCAGTGAAAATTATTGCAGTACGTTGGTGATGCAGGTGCAGGGGAGTCAACTGACTAATA
AGTATGCTGAAGGGTATTCTGGAAAGCGTTACTACGGTGGTTGTGAGTATGTCGATATCGCTGAGCAATTGGCGATTGAG
CGTGCCAAAAAACTGTTTGGTGCCGATTATGCCAATGTGCAGCCCCATAGCGGTTCACAAGCCAATCAAGCGGTGTACTT
TGCCTTGTTGCAGCCAGGCGATACGATCCTCGGGATGAGCTTGGCGCATGGCGGTCACTTGACTCACGGCGCCAATGTTA
ATGTGTCTGGCAAGTTGTTCAATGCGGTTCAGTACGGAGTGAATGCTCAAGGGTTGATTGATTACGAGGCTGTTGAATCG
CTTGCGTTAGAGCATCGGCCGAAGATGGTTGTGGCGGGCTTTTCGGCGTATTCGCAGAAAATTGATTGGGCGCGCTTCCG
TGCCATTGCTGATCAGGTGGGCGCTTACTTGCTTGTTGATATGGCACATGTGGCTGGCTTGGTGGCGGCTGGTGTCTATC
CGAGTCCGCTGCCGCATGCGCATGTTGTGACTTCGACGACGCATAAGACACTGCGCGGCCCACGTGGCGGCATTATTGTT
GCGCAGGCTCCGCAGGAGGCGTTGGTTAAGAAGTTGCAGTCGATAGTGTTTCCTGGGATCCAGGGTGGTCCATTGATGCA
TGTGATCGCGGCCAAAGCGGTTGCTTTTAAGGAGGCGTTGGAGCCTGCGTTTAAGGTGTATCAGCAGCAGGTGGTGAAGA
ATGCGAAGGCGATGGCTGGGACCCTCATGTTACGCGGCTATAAGATTGTCTCTGGTGGTACAGAAAATCATTTGATGTTG
GTTGACATGATCGGTAGGGATGTCTCTGGGAAGGACGCTGAGGGCGCATTGGGTCAGGTTCATATCACTGTCAATAAGAA
TGCGGTACCGGATGATCCGCGCTCTCCGTTCGTAACCTCCGGGTTACGCTTGGGGACTCCAGCAGTCACGACACGGGGTT
ACCAGGAGCAGGATTGTGTGGACTTAGCGCACTGGATTGCTGATGTGCTTGACGCGCCTGCAGATGTGACGGTCATTGCC
GCAGTCCGTGAGAAGGTTGCGGCGCAGTGCAAGAAGTATCCTGTGTATCGCTGA

Upstream 100 bases:

>100_bases
CGTGTAGGCTGATGAATTTGCTCAGTCCAGTGTGGTGCGTTTTATAATTTTTGGTGTCCAGATGCTGATTGCATCCCCTG
TGTTTTTTTCCGGAGTTGCC

Downstream 100 bases:

>100_bases
GTGTCTGGGGTTGGAATGTTACGGTGCTTGGAACGCAGTGACGTTTCTTGGATATTCTTGCTGTGCTTCAGTGAAGGATA
TTTGGGTGATGATGCGTTTT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MFPRDARLDMYDPELAKAIAAEVRRQEDHVELIASENYCSTLVMQVQGSQLTNKYAEGYSGKRYYGGCEYVDIAEQLAIE
RAKKLFGADYANVQPHSGSQANQAVYFALLQPGDTILGMSLAHGGHLTHGANVNVSGKLFNAVQYGVNAQGLIDYEAVES
LALEHRPKMVVAGFSAYSQKIDWARFRAIADQVGAYLLVDMAHVAGLVAAGVYPSPLPHAHVVTSTTHKTLRGPRGGIIV
AQAPQEALVKKLQSIVFPGIQGGPLMHVIAAKAVAFKEALEPAFKVYQQQVVKNAKAMAGTLMLRGYKIVSGGTENHLML
VDMIGRDVSGKDAEGALGQVHITVNKNAVPDDPRSPFVTSGLRLGTPAVTTRGYQEQDCVDLAHWIADVLDAPADVTVIA
AVREKVAAQCKKYPVYR

Sequences:

>Translated_417_residues
MFPRDARLDMYDPELAKAIAAEVRRQEDHVELIASENYCSTLVMQVQGSQLTNKYAEGYSGKRYYGGCEYVDIAEQLAIE
RAKKLFGADYANVQPHSGSQANQAVYFALLQPGDTILGMSLAHGGHLTHGANVNVSGKLFNAVQYGVNAQGLIDYEAVES
LALEHRPKMVVAGFSAYSQKIDWARFRAIADQVGAYLLVDMAHVAGLVAAGVYPSPLPHAHVVTSTTHKTLRGPRGGIIV
AQAPQEALVKKLQSIVFPGIQGGPLMHVIAAKAVAFKEALEPAFKVYQQQVVKNAKAMAGTLMLRGYKIVSGGTENHLML
VDMIGRDVSGKDAEGALGQVHITVNKNAVPDDPRSPFVTSGLRLGTPAVTTRGYQEQDCVDLAHWIADVLDAPADVTVIA
AVREKVAAQCKKYPVYR
>Mature_417_residues
MFPRDARLDMYDPELAKAIAAEVRRQEDHVELIASENYCSTLVMQVQGSQLTNKYAEGYSGKRYYGGCEYVDIAEQLAIE
RAKKLFGADYANVQPHSGSQANQAVYFALLQPGDTILGMSLAHGGHLTHGANVNVSGKLFNAVQYGVNAQGLIDYEAVES
LALEHRPKMVVAGFSAYSQKIDWARFRAIADQVGAYLLVDMAHVAGLVAAGVYPSPLPHAHVVTSTTHKTLRGPRGGIIV
AQAPQEALVKKLQSIVFPGIQGGPLMHVIAAKAVAFKEALEPAFKVYQQQVVKNAKAMAGTLMLRGYKIVSGGTENHLML
VDMIGRDVSGKDAEGALGQVHITVNKNAVPDDPRSPFVTSGLRLGTPAVTTRGYQEQDCVDLAHWIADVLDAPADVTVIA
AVREKVAAQCKKYPVYR

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=397, Percent_Identity=48.6146095717884, Blast_Score=352, Evalue=4e-97,
Organism=Homo sapiens, GI261862350, Length=397, Percent_Identity=48.6146095717884, Blast_Score=352, Evalue=4e-97,
Organism=Homo sapiens, GI261862348, Length=397, Percent_Identity=48.6146095717884, Blast_Score=352, Evalue=4e-97,
Organism=Homo sapiens, GI19923315, Length=397, Percent_Identity=48.6146095717884, Blast_Score=352, Evalue=5e-97,
Organism=Homo sapiens, GI261862346, Length=388, Percent_Identity=48.1958762886598, Blast_Score=338, Evalue=6e-93,
Organism=Homo sapiens, GI22547186, Length=419, Percent_Identity=46.3007159904535, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI22547189, Length=407, Percent_Identity=44.7174447174447, Blast_Score=294, Evalue=1e-79,
Organism=Escherichia coli, GI1788902, Length=416, Percent_Identity=67.5480769230769, Blast_Score=573, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI25144732, Length=396, Percent_Identity=46.4646464646465, Blast_Score=340, Evalue=8e-94,
Organism=Caenorhabditis elegans, GI25144729, Length=396, Percent_Identity=46.4646464646465, Blast_Score=340, Evalue=1e-93,
Organism=Saccharomyces cerevisiae, GI6319739, Length=398, Percent_Identity=46.9849246231156, Blast_Score=349, Evalue=4e-97,
Organism=Saccharomyces cerevisiae, GI6323087, Length=397, Percent_Identity=44.5843828715365, Blast_Score=328, Evalue=1e-90,
Organism=Drosophila melanogaster, GI24640005, Length=411, Percent_Identity=47.9318734793187, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI221329721, Length=411, Percent_Identity=47.9318734793187, Blast_Score=362, Evalue=1e-100,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_XYLF2 (B2I8R0)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830525.1
- ProteinModelPortal:   B2I8R0
- SMR:   B2I8R0
- GeneID:   6201885
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1851
- HOGENOM:   HBG301263
- OMA:   PEEMNID
- ProtClustDB:   PRK00011
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 44995; Mature: 44995

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPRDARLDMYDPELAKAIAAEVRRQEDHVELIASENYCSTLVMQVQGSQLTNKYAEGYS
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHEEECCCHHHHHHHCCCC
GKRYYGGCEYVDIAEQLAIERAKKLFGADYANVQPHSGSQANQAVYFALLQPGDTILGMS
CCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEE
LAHGGHLTHGANVNVSGKLFNAVQYGVNAQGLIDYEAVESLALEHRPKMVVAGFSAYSQK
ECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEECHHHHHHHHHHCCCCEEEEECHHHHHH
IDWARFRAIADQVGAYLLVDMAHVAGLVAAGVYPSPLPHAHVVTSTTHKTLRGPRGGIIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHCCCCCCEEE
AQAPQEALVKKLQSIVFPGIQGGPLMHVIAAKAVAFKEALEPAFKVYQQQVVKNAKAMAG
EECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLMLRGYKIVSGGTENHLMLVDMIGRDVSGKDAEGALGQVHITVNKNAVPDDPRSPFVTS
HHHHCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHC
GLRLGTPAVTTRGYQEQDCVDLAHWIADVLDAPADVTVIAAVREKVAAQCKKYPVYR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFPRDARLDMYDPELAKAIAAEVRRQEDHVELIASENYCSTLVMQVQGSQLTNKYAEGYS
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHEEECCCHHHHHHHCCCC
GKRYYGGCEYVDIAEQLAIERAKKLFGADYANVQPHSGSQANQAVYFALLQPGDTILGMS
CCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEE
LAHGGHLTHGANVNVSGKLFNAVQYGVNAQGLIDYEAVESLALEHRPKMVVAGFSAYSQK
ECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEECHHHHHHHHHHCCCCEEEEECHHHHHH
IDWARFRAIADQVGAYLLVDMAHVAGLVAAGVYPSPLPHAHVVTSTTHKTLRGPRGGIIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHCCCCCCEEE
AQAPQEALVKKLQSIVFPGIQGGPLMHVIAAKAVAFKEALEPAFKVYQQQVVKNAKAMAG
EECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLMLRGYKIVSGGTENHLMLVDMIGRDVSGKDAEGALGQVHITVNKNAVPDDPRSPFVTS
HHHHCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHC
GLRLGTPAVTTRGYQEQDCVDLAHWIADVLDAPADVTVIAAVREKVAAQCKKYPVYR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA