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The map label for this gene is dnaB [H]

Identifier: 182682311

GI number: 182682311

Start: 1995164

End: 1996579

Strand: Reverse

Name: dnaB [H]

Synonym: XfasM23_1794

Alternate gene names: 182682311

Gene position: 1996579-1995164 (Counterclockwise)

Preceding gene: 182682312

Following gene: 182682310

Centisome position: 78.74

GC content: 50.42

Gene sequence:

>1416_bases
ATGTCTGCTCGTCCCGGCTTCCATTCCAATTATCAACGCGAGCCTGGCGATCGTGAAGCGCTGCGTGTTGATCCGTTGCG
CGTCCCTCCTCATTCTGTTGAGGCTGAGCAGGCCGTATTGGGGGGGCTGATGTTGGCGCCCGATGCGTTGGATAAGATCA
ATGATCGGTTAACGGAGAACGATTTTTACCGTCGTGATCACCGCATGATTTACCGTGCGATCTGCGATTTGTCTAGGAAG
ATGCGACCATTCGATGTCGTCACTCTAGGAGAATGGTTTGAGTCGCAGGGGATGCTGGAGGTTGTTGATCATGGTGCTTA
TTTGATTGAGCTGGCGAGTACCACGCCCTCGGCCGCCAATATCGTTGCCTATGCTGAAATCGTGCGCGATAAGGCAGTAC
TGCGTCAGTTGATTGAGGTTGGAACCAAGATCGTTAACGATGGATTCCAACCAGAGGGCCGCGAAAGTATTGATTTGCTT
GCTGAGGCTGAAAAGTCGGTGTTCGGAATTGCCGAGACTGGTGCGCGTGGTCGCATTGATTTTGTGGCAATGCCTGGTGC
GTTGAAGGATGCTTTTGAGCAGCTGCGTGATCGTTTTGAGAACGGTAGTAATGTGACCGGGCTTCCAACCGGATATAACG
ATTTTGACGCGATGAGCGCAGGTTTACAGCCGACTGATTTGATCGTCTTGGCTGCGCGTCCGGCAATGGGGAAAACAACC
TTTGCGTTGAATATCGCTGAGTATGTTGCGATTAAATCGAAAAAGACGGTGGCTGTATTTTCAATGGAAATGTCTGCTGC
ACAGTTGGCGATGCGCTTGATTTCTTCTAATGGTCGTATCAATGCGCAGCGTCTGCGTACGGGTCAGCTTGAGGACGAAG
ATTGGGCACGTGTGACTGGAGCAATCAAGATGTTGAAGGAGACTAAAATTTTTATTGATGATACCCCTGGTGTGTCGCCA
GAGGTATTGCGCTCCAAGTGCCGTCGTTTGAAGCGTGAGCATGATCTTGGTCTAGTGGTGGTCGATTATCTGCAGTTGAT
GTCTGTCCCTGGGAACAGTGAGAACCGTGCTACCGAGATCTCGGAAATTTCGCGCTCTCTCAAGGGGTTGGCTAAGGAGT
TGAATGTTCCAGTGATTGCATTGTCACAGTTGAACCGTTCTTTGGAGACGCGCACTGACAAGCATCCCGTCATGGCTGAT
CTGCGTGAATCTGGTGCGATTGAGCAGGATGCAGACATGATCGTTTTTATCTACCGGGATGAGTATTACAACAAAGATTC
TCCGGATAAGGGTTTGGCGGAAATCATCATCGGTAAGCATCGCAGCGGCCCGACAGGTTCGTGCAAGTTGAAGTTCTTTG
GCGAATACACCCGTTTCGATAATCTGGCCCACGACTCAATTGGCACATTTGAGTAG

Upstream 100 bases:

>100_bases
CACTGACTGTCACCGGTTTATCCACAGGATTGTACCTTGGCCGTTGCTGCGGTACTGACTATGCTTCACCCCCTGTTACA
CAGTACTAGGCCGTTGTTTT

Downstream 100 bases:

>100_bases
TACGGCGTTGCTGGAGATCTAGGGGGCTAAATCGGCGCATCTATCCTTGTGACACTCTTGGTCTCGCGTTGCTACGGGTG
CAATAGACGGTGTGTAGTCA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSARPGFHSNYQREPGDREALRVDPLRVPPHSVEAEQAVLGGLMLAPDALDKINDRLTENDFYRRDHRMIYRAICDLSRK
MRPFDVVTLGEWFESQGMLEVVDHGAYLIELASTTPSAANIVAYAEIVRDKAVLRQLIEVGTKIVNDGFQPEGRESIDLL
AEAEKSVFGIAETGARGRIDFVAMPGALKDAFEQLRDRFENGSNVTGLPTGYNDFDAMSAGLQPTDLIVLAARPAMGKTT
FALNIAEYVAIKSKKTVAVFSMEMSAAQLAMRLISSNGRINAQRLRTGQLEDEDWARVTGAIKMLKETKIFIDDTPGVSP
EVLRSKCRRLKREHDLGLVVVDYLQLMSVPGNSENRATEISEISRSLKGLAKELNVPVIALSQLNRSLETRTDKHPVMAD
LRESGAIEQDADMIVFIYRDEYYNKDSPDKGLAEIIIGKHRSGPTGSCKLKFFGEYTRFDNLAHDSIGTFE

Sequences:

>Translated_471_residues
MSARPGFHSNYQREPGDREALRVDPLRVPPHSVEAEQAVLGGLMLAPDALDKINDRLTENDFYRRDHRMIYRAICDLSRK
MRPFDVVTLGEWFESQGMLEVVDHGAYLIELASTTPSAANIVAYAEIVRDKAVLRQLIEVGTKIVNDGFQPEGRESIDLL
AEAEKSVFGIAETGARGRIDFVAMPGALKDAFEQLRDRFENGSNVTGLPTGYNDFDAMSAGLQPTDLIVLAARPAMGKTT
FALNIAEYVAIKSKKTVAVFSMEMSAAQLAMRLISSNGRINAQRLRTGQLEDEDWARVTGAIKMLKETKIFIDDTPGVSP
EVLRSKCRRLKREHDLGLVVVDYLQLMSVPGNSENRATEISEISRSLKGLAKELNVPVIALSQLNRSLETRTDKHPVMAD
LRESGAIEQDADMIVFIYRDEYYNKDSPDKGLAEIIIGKHRSGPTGSCKLKFFGEYTRFDNLAHDSIGTFE
>Mature_470_residues
SARPGFHSNYQREPGDREALRVDPLRVPPHSVEAEQAVLGGLMLAPDALDKINDRLTENDFYRRDHRMIYRAICDLSRKM
RPFDVVTLGEWFESQGMLEVVDHGAYLIELASTTPSAANIVAYAEIVRDKAVLRQLIEVGTKIVNDGFQPEGRESIDLLA
EAEKSVFGIAETGARGRIDFVAMPGALKDAFEQLRDRFENGSNVTGLPTGYNDFDAMSAGLQPTDLIVLAARPAMGKTTF
ALNIAEYVAIKSKKTVAVFSMEMSAAQLAMRLISSNGRINAQRLRTGQLEDEDWARVTGAIKMLKETKIFIDDTPGVSPE
VLRSKCRRLKREHDLGLVVVDYLQLMSVPGNSENRATEISEISRSLKGLAKELNVPVIALSQLNRSLETRTDKHPVMADL
RESGAIEQDADMIVFIYRDEYYNKDSPDKGLAEIIIGKHRSGPTGSCKLKFFGEYTRFDNLAHDSIGTFE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=457, Percent_Identity=53.3916849015317, Blast_Score=473, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 52389; Mature: 52258

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARPGFHSNYQREPGDREALRVDPLRVPPHSVEAEQAVLGGLMLAPDALDKINDRLTEN
CCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHCCHHCCCHHHHHHHHHHCCC
DFYRRDHRMIYRAICDLSRKMRPFDVVTLGEWFESQGMLEVVDHGAYLIELASTTPSAAN
HHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCHHHHHHCCEEEEEEECCCCCHHH
IVAYAEIVRDKAVLRQLIEVGTKIVNDGFQPEGRESIDLLAEAEKSVFGIAETGARGRID
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHEEECCCCCCCCEE
FVAMPGALKDAFEQLRDRFENGSNVTGLPTGYNDFDAMSAGLQPTDLIVLAARPAMGKTT
EEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCCCCCE
FALNIAEYVAIKSKKTVAVFSMEMSAAQLAMRLISSNGRINAQRLRTGQLEDEDWARVTG
EEEEHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHCCCCEEHHHHCCCCCCCHHHHHHHH
AIKMLKETKIFIDDTPGVSPEVLRSKCRRLKREHDLGLVVVDYLQLMSVPGNSENRATEI
HHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHH
SEISRSLKGLAKELNVPVIALSQLNRSLETRTDKHPVMADLRESGAIEQDADMIVFIYRD
HHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEEEEC
EYYNKDSPDKGLAEIIIGKHRSGPTGSCKLKFFGEYTRFDNLAHDSIGTFE
CCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEEEECHHHHCCHHCCCCCCCC
>Mature Secondary Structure 
SARPGFHSNYQREPGDREALRVDPLRVPPHSVEAEQAVLGGLMLAPDALDKINDRLTEN
CCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHCCHHCCCHHHHHHHHHHCCC
DFYRRDHRMIYRAICDLSRKMRPFDVVTLGEWFESQGMLEVVDHGAYLIELASTTPSAAN
HHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCHHHHHHCCEEEEEEECCCCCHHH
IVAYAEIVRDKAVLRQLIEVGTKIVNDGFQPEGRESIDLLAEAEKSVFGIAETGARGRID
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHEEECCCCCCCCEE
FVAMPGALKDAFEQLRDRFENGSNVTGLPTGYNDFDAMSAGLQPTDLIVLAARPAMGKTT
EEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCCCCCE
FALNIAEYVAIKSKKTVAVFSMEMSAAQLAMRLISSNGRINAQRLRTGQLEDEDWARVTG
EEEEHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHCCCCEEHHHHCCCCCCCHHHHHHHH
AIKMLKETKIFIDDTPGVSPEVLRSKCRRLKREHDLGLVVVDYLQLMSVPGNSENRATEI
HHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHH
SEISRSLKGLAKELNVPVIALSQLNRSLETRTDKHPVMADLRESGAIEQDADMIVFIYRD
HHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEEEEC
EYYNKDSPDKGLAEIIIGKHRSGPTGSCKLKFFGEYTRFDNLAHDSIGTFE
CCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEEEECHHHHCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]