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The map label for this gene is metK

Identifier: 182682288

GI number: 182682288

Start: 1960526

End: 1961737

Strand: Reverse

Name: metK

Synonym: XfasM23_1771

Alternate gene names: 182682288

Gene position: 1961737-1960526 (Counterclockwise)

Preceding gene: 182682289

Following gene: 182682287

Centisome position: 77.37

GC content: 47.44

Gene sequence:

>1212_bases
ATGTCCAGCTACCTTTTTACATCCGAATCGGTTTCGGAAGGCCATCCGGATAAGGTTGCCGACCAAATTTCTGACGCAGT
GCTCGATGCGATCTTGGTTCAGGATCCGCGTGCCCGTGTTGCTTGCGAGACAATGGTTAAGACTGGTGTAGCGATCATCG
CTGGTGAAATTACTACAAGTGCCTGGGTTGATCTGGAAGCGTTAACCCGTAAAGTGATTGTTGACATCGGCTATAACAGT
TCCGACGTTGGTTTCGATGGTGCTACTTGCGGCGTGTTGAACTTAATTGGTAAGCAGTCCCCAGATATTAATCAGGGAGT
GGATCGTAAAAAGGCTGAGGAACAGGGAGCTGGTGATCAGGGCTTGATGTTCGGATATGCCACCAACGAAACTGATAGCT
ATATGCCAGCGGCAATTCACATATCACACCGGCTAGTTGAGCAGCAAGCAAAAATTCGTAAAAAAAGCAACTCGCTATTG
CCGTGGTTACGTCCGGATGCTAAGGCTCAGATTACTTTGCGTTATGAGGACGGTGTGGCCAGTGCGATTGATGCTGTTGT
GCTATCAACCCAGCACGATCCTGGTATCAAACAGAAGGATCTTGTTGAAGCAGTGCGTGAGGAAATTCTCAAGCCTGTGT
TGCCTTCCAAGTGGTTGCACAAAGGAACCAAATTCCACATTAATCCAACGGGTAAATTTGTGATTGGTGGTCCGGTGGGC
GATTGTGGTCTGACTGGCCGCAAGATCATTGTTGACACGTATGGCGGTTCAGCCCGTCATGGCGGCGGTGCTTTCTCTGG
TAAAGATCCATCAAAAGTAGATCGTTCGGCTTCTTATGCTGTCCGTTATGTTGCTAAAAACGTGGTTGCTGCTGGTTTAG
CTGATCGTTGCGAAGTGCAGGTATCCTATGCAATTGGTGTGGCTGAGCCTATTTCTATCTCAGTGACTACTTTTGGTACA
GGTAAGATACCCGATGATAAAATCGAGAAACTAATCTGCCAGCATTTTGATCTTCGTCCCTATGGCATTATCAAAATGTT
AGATTTGATTCATCCGATATATCAACCGAGTGCTAGTTACGGTCATTTTGGCCGTAAGCCGAGAGAGTTGGCTTACACCA
ACAGCAACGGTGAACGTGTGGTTGCCACTGCTTTCTCGTGGGAGAAAATCGATAAGGCAGAAGTACTCCGTGCTGATGCT
AAACTGGAGTAG

Upstream 100 bases:

>100_bases
TCAAAGATGCTTGATTGAAATGAACAATACAGATTGGTTGCATTGAGCGTATCATAGATGCTTACTTATCTTTTCATTCG
AATTAAGGGATATAGACCTG

Downstream 100 bases:

>100_bases
GTCTTTTTTCCATCAATAGCGAGTGATGAGCAAACATAATCCGTTTATAAGTTTGGCTGCCCCGGATGGATTCGAACCAA
CGAATGTCTGAGTCAGAGTC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSSYLFTSESVSEGHPDKVADQISDAVLDAILVQDPRARVACETMVKTGVAIIAGEITTSAWVDLEALTRKVIVDIGYNS
SDVGFDGATCGVLNLIGKQSPDINQGVDRKKAEEQGAGDQGLMFGYATNETDSYMPAAIHISHRLVEQQAKIRKKSNSLL
PWLRPDAKAQITLRYEDGVASAIDAVVLSTQHDPGIKQKDLVEAVREEILKPVLPSKWLHKGTKFHINPTGKFVIGGPVG
DCGLTGRKIIVDTYGGSARHGGGAFSGKDPSKVDRSASYAVRYVAKNVVAAGLADRCEVQVSYAIGVAEPISISVTTFGT
GKIPDDKIEKLICQHFDLRPYGIIKMLDLIHPIYQPSASYGHFGRKPRELAYTNSNGERVVATAFSWEKIDKAEVLRADA
KLE

Sequences:

>Translated_403_residues
MSSYLFTSESVSEGHPDKVADQISDAVLDAILVQDPRARVACETMVKTGVAIIAGEITTSAWVDLEALTRKVIVDIGYNS
SDVGFDGATCGVLNLIGKQSPDINQGVDRKKAEEQGAGDQGLMFGYATNETDSYMPAAIHISHRLVEQQAKIRKKSNSLL
PWLRPDAKAQITLRYEDGVASAIDAVVLSTQHDPGIKQKDLVEAVREEILKPVLPSKWLHKGTKFHINPTGKFVIGGPVG
DCGLTGRKIIVDTYGGSARHGGGAFSGKDPSKVDRSASYAVRYVAKNVVAAGLADRCEVQVSYAIGVAEPISISVTTFGT
GKIPDDKIEKLICQHFDLRPYGIIKMLDLIHPIYQPSASYGHFGRKPRELAYTNSNGERVVATAFSWEKIDKAEVLRADA
KLE
>Mature_402_residues
SSYLFTSESVSEGHPDKVADQISDAVLDAILVQDPRARVACETMVKTGVAIIAGEITTSAWVDLEALTRKVIVDIGYNSS
DVGFDGATCGVLNLIGKQSPDINQGVDRKKAEEQGAGDQGLMFGYATNETDSYMPAAIHISHRLVEQQAKIRKKSNSLLP
WLRPDAKAQITLRYEDGVASAIDAVVLSTQHDPGIKQKDLVEAVREEILKPVLPSKWLHKGTKFHINPTGKFVIGGPVGD
CGLTGRKIIVDTYGGSARHGGGAFSGKDPSKVDRSASYAVRYVAKNVVAAGLADRCEVQVSYAIGVAEPISISVTTFGTG
KIPDDKIEKLICQHFDLRPYGIIKMLDLIHPIYQPSASYGHFGRKPRELAYTNSNGERVVATAFSWEKIDKAEVLRADAK
LE

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=380, Percent_Identity=53.6842105263158, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI5174529, Length=381, Percent_Identity=53.0183727034121, Blast_Score=385, Evalue=1e-107,
Organism=Escherichia coli, GI1789311, Length=403, Percent_Identity=68.4863523573201, Blast_Score=573, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI17551082, Length=369, Percent_Identity=54.4715447154472, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17538498, Length=378, Percent_Identity=54.4973544973545, Blast_Score=386, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17538494, Length=378, Percent_Identity=54.4973544973545, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25145633, Length=408, Percent_Identity=51.7156862745098, Blast_Score=383, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI32565905, Length=378, Percent_Identity=48.6772486772487, Blast_Score=324, Evalue=5e-89,
Organism=Caenorhabditis elegans, GI32565911, Length=330, Percent_Identity=51.2121212121212, Blast_Score=311, Evalue=5e-85,
Organism=Caenorhabditis elegans, GI25145635, Length=358, Percent_Identity=48.6033519553073, Blast_Score=306, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI32565903, Length=305, Percent_Identity=50.4918032786885, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI32565909, Length=82, Percent_Identity=64.6341463414634, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI32565907, Length=45, Percent_Identity=73.3333333333333, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320710, Length=397, Percent_Identity=53.1486146095718, Blast_Score=386, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6323209, Length=381, Percent_Identity=53.5433070866142, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45552159, Length=399, Percent_Identity=50.8771929824561, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580529, Length=399, Percent_Identity=50.8771929824561, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580537, Length=399, Percent_Identity=50.8771929824561, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580533, Length=399, Percent_Identity=50.8771929824561, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580535, Length=399, Percent_Identity=50.8771929824561, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580531, Length=399, Percent_Identity=50.8771929824561, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580527, Length=399, Percent_Identity=50.3759398496241, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24580525, Length=399, Percent_Identity=50.3759398496241, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24580541, Length=398, Percent_Identity=48.4924623115578, Blast_Score=335, Evalue=3e-92,
Organism=Drosophila melanogaster, GI24580539, Length=120, Percent_Identity=55, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_XYLF2 (B2I898)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830448.1
- ProteinModelPortal:   B2I898
- SMR:   B2I898
- GeneID:   6201966
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1771
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 43608; Mature: 43477

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSYLFTSESVSEGHPDKVADQISDAVLDAILVQDPRARVACETMVKTGVAIIAGEITTS
CCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEEECCCHH
AWVDLEALTRKVIVDIGYNSSDVGFDGATCGVLNLIGKQSPDINQGVDRKKAEEQGAGDQ
HHEEHHHHHEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCC
GLMFGYATNETDSYMPAAIHISHRLVEQQAKIRKKSNSLLPWLRPDAKAQITLRYEDGVA
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCHH
SAIDAVVLSTQHDPGIKQKDLVEAVREEILKPVLPSKWLHKGTKFHINPTGKFVIGGPVG
HHHHHHHEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCEEEECCCCCEEECCCCC
DCGLTGRKIIVDTYGGSARHGGGAFSGKDPSKVDRSASYAVRYVAKNVVAAGLADRCEVQ
CCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
VSYAIGVAEPISISVTTFGTGKIPDDKIEKLICQHFDLRPYGIIKMLDLIHPIYQPSASY
EEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCC
GHFGRKPRELAYTNSNGERVVATAFSWEKIDKAEVLRADAKLE
CCCCCCCCCEEEECCCCCEEEEEECCHHHCCHHHHHHHCCCCC
>Mature Secondary Structure 
SSYLFTSESVSEGHPDKVADQISDAVLDAILVQDPRARVACETMVKTGVAIIAGEITTS
CCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEEECCCHH
AWVDLEALTRKVIVDIGYNSSDVGFDGATCGVLNLIGKQSPDINQGVDRKKAEEQGAGDQ
HHEEHHHHHEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCC
GLMFGYATNETDSYMPAAIHISHRLVEQQAKIRKKSNSLLPWLRPDAKAQITLRYEDGVA
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCHH
SAIDAVVLSTQHDPGIKQKDLVEAVREEILKPVLPSKWLHKGTKFHINPTGKFVIGGPVG
HHHHHHHEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCEEEECCCCCEEECCCCC
DCGLTGRKIIVDTYGGSARHGGGAFSGKDPSKVDRSASYAVRYVAKNVVAAGLADRCEVQ
CCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
VSYAIGVAEPISISVTTFGTGKIPDDKIEKLICQHFDLRPYGIIKMLDLIHPIYQPSASY
EEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCC
GHFGRKPRELAYTNSNGERVVATAFSWEKIDKAEVLRADAKLE
CCCCCCCCCEEEECCCCCEEEEEECCHHHCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA