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The map label for this gene is gca [H]

Identifier: 182682151

GI number: 182682151

Start: 1806585

End: 1807625

Strand: Reverse

Name: gca [H]

Synonym: XfasM23_1630

Alternate gene names: 182682151

Gene position: 1807625-1806585 (Counterclockwise)

Preceding gene: 182682152

Following gene: 182682150

Centisome position: 71.29

GC content: 46.97

Gene sequence:

>1041_bases
ATGTGTAATAAAAGCGCGGTCATTACGGGTATTACAGGACAGGATGGTGCTTATTTAGCGCAGTTGCTCCTCGAAATGGG
TTATGTGGTTTATGGTACGTATCGTCGTTCTAGCTCATTGAACTTCTGGCGTATTAATGAACTTGGGATTGCACAGCATC
CCAATTTACATCTGGTCGAATATGATTTGACCGATCTCTCTTCGAGTGTGAGATTGCTCGAATTGGCCGCTCCTAAAGAG
GTCTATAATCTTGCCGCACAGAGCTTTGTTGGCGTCTCTTTTGACCAGCCATTGACCACGACCGATATTACCGGAGTGGG
TGCGGTGAACCTGTTGGAAGCCATTCGGATTGTTAATCCTAAGATTCGCTTCTATCAGGCATCGACTTCTGAGATGTTTG
GAAAAGTCCAGCAGACTCCGCAGATTGAGGAAACGCCTTTCTATCCGCGCAGCCCATACGGTATTGCCAAGTTGTATGCT
CACTGGATGACGGTTAATTATCGTGAGTCGTACGGTATCTTTGCTGCTAGTGGTATTTTGTTTAATCACGAATCGCCGTT
GCGAGCGCGTGATTTTGTTACTCGTAAAATCACTCATGCGATTGCCATGATTAAATTAGGGAAGCTAGACGTATTAGAGC
TTGGTAATTTGGATGCCAAACGTGATTGGGGTTTTGCGAAGGAATATGTTGAGGGAATGTGGCGTATGCTTCAGGCTGAT
GAGCCAGATACTTTCGTGCTGGCAACCAACCGAACTGAAACTGTGCGTGATTTCGTCAGCTTGGCTGCCAGTGCCGTTGA
TATCCAGTTGGAGTGGAAGGGACAAGCCGAACGGGAATGTGGTGTTGATGTTGCATCGGGAAAAACTTTAGTCACAATCA
ATCCTAAGTTTTATCGACCGGCTGAGGTGGATTTGTTGATCGGTAATCCGGCCAAAGCTAGAACGAAGCTGGGTTGGGAA
CCAAAGACCACACTTGAGGAGCTTTGTCACATGATGATGGAAGCAGATGTGCGCCGCAGCGAACGCGATTTCCCCTTCTA
A

Upstream 100 bases:

>100_bases
GAGATGTGAGTGAATGTGTTTATAATTCTTTTTTGAGATAGATTCACCATTAATAAAGTGATGTTTATTTCTTTTTCAAT
CATGTTTTGAAGAAATACTA

Downstream 100 bases:

>100_bases
ATGAATTTTTCTGGTAAGCGTGTTCTATTGACAGGTAGCAGCGGTTTTACCGGTCGATATATGCTGGACGAGCTTCTTAG
CCATGGTTACGAAGTCATTG

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MCNKSAVITGITGQDGAYLAQLLLEMGYVVYGTYRRSSSLNFWRINELGIAQHPNLHLVEYDLTDLSSSVRLLELAAPKE
VYNLAAQSFVGVSFDQPLTTTDITGVGAVNLLEAIRIVNPKIRFYQASTSEMFGKVQQTPQIEETPFYPRSPYGIAKLYA
HWMTVNYRESYGIFAASGILFNHESPLRARDFVTRKITHAIAMIKLGKLDVLELGNLDAKRDWGFAKEYVEGMWRMLQAD
EPDTFVLATNRTETVRDFVSLAASAVDIQLEWKGQAERECGVDVASGKTLVTINPKFYRPAEVDLLIGNPAKARTKLGWE
PKTTLEELCHMMMEADVRRSERDFPF

Sequences:

>Translated_346_residues
MCNKSAVITGITGQDGAYLAQLLLEMGYVVYGTYRRSSSLNFWRINELGIAQHPNLHLVEYDLTDLSSSVRLLELAAPKE
VYNLAAQSFVGVSFDQPLTTTDITGVGAVNLLEAIRIVNPKIRFYQASTSEMFGKVQQTPQIEETPFYPRSPYGIAKLYA
HWMTVNYRESYGIFAASGILFNHESPLRARDFVTRKITHAIAMIKLGKLDVLELGNLDAKRDWGFAKEYVEGMWRMLQAD
EPDTFVLATNRTETVRDFVSLAASAVDIQLEWKGQAERECGVDVASGKTLVTINPKFYRPAEVDLLIGNPAKARTKLGWE
PKTTLEELCHMMMEADVRRSERDFPF
>Mature_346_residues
MCNKSAVITGITGQDGAYLAQLLLEMGYVVYGTYRRSSSLNFWRINELGIAQHPNLHLVEYDLTDLSSSVRLLELAAPKE
VYNLAAQSFVGVSFDQPLTTTDITGVGAVNLLEAIRIVNPKIRFYQASTSEMFGKVQQTPQIEETPFYPRSPYGIAKLYA
HWMTVNYRESYGIFAASGILFNHESPLRARDFVTRKITHAIAMIKLGKLDVLELGNLDAKRDWGFAKEYVEGMWRMLQAD
EPDTFVLATNRTETVRDFVSLAASAVDIQLEWKGQAERECGVDVASGKTLVTINPKFYRPAEVDLLIGNPAKARTKLGWE
PKTTLEELCHMMMEADVRRSERDFPF

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=341, Percent_Identity=52.4926686217009, Blast_Score=361, Evalue=1e-100,
Organism=Escherichia coli, GI1788366, Length=355, Percent_Identity=49.8591549295775, Blast_Score=356, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI17507723, Length=349, Percent_Identity=53.2951289398281, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI133901788, Length=341, Percent_Identity=52.4926686217009, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17539422, Length=341, Percent_Identity=52.4926686217009, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17539424, Length=341, Percent_Identity=52.4926686217009, Blast_Score=359, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI133901790, Length=341, Percent_Identity=52.4926686217009, Blast_Score=359, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI133901786, Length=341, Percent_Identity=52.4926686217009, Blast_Score=359, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24158427, Length=343, Percent_Identity=50.7288629737609, Blast_Score=355, Evalue=3e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 38979; Mature: 38979

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCNKSAVITGITGQDGAYLAQLLLEMGYVVYGTYRRSSSLNFWRINELGIAQHPNLHLVE
CCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCEEEECCCCCCCCCCEEEEE
YDLTDLSSSVRLLELAAPKEVYNLAAQSFVGVSFDQPLTTTDITGVGAVNLLEAIRIVNP
EEHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
KIRFYQASTSEMFGKVQQTPQIEETPFYPRSPYGIAKLYAHWMTVNYRESYGIFAASGIL
CEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHEEEECCCCCEEEECEEE
FNHESPLRARDFVTRKITHAIAMIKLGKLDVLELGNLDAKRDWGFAKEYVEGMWRMLQAD
ECCCCCCHHHHHHHHHHHHHHHHHEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
EPDTFVLATNRTETVRDFVSLAASAVDIQLEWKGQAERECGVDVASGKTLVTINPKFYRP
CCCEEEEECCCHHHHHHHHHHHHHEEEEEEEECCCCCHHCCCEECCCCEEEEECCCCCCC
AEVDLLIGNPAKARTKLGWEPKTTLEELCHMMMEADVRRSERDFPF
CEEEEEECCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MCNKSAVITGITGQDGAYLAQLLLEMGYVVYGTYRRSSSLNFWRINELGIAQHPNLHLVE
CCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCEEEECCCCCCCCCCEEEEE
YDLTDLSSSVRLLELAAPKEVYNLAAQSFVGVSFDQPLTTTDITGVGAVNLLEAIRIVNP
EEHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
KIRFYQASTSEMFGKVQQTPQIEETPFYPRSPYGIAKLYAHWMTVNYRESYGIFAASGIL
CEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHEEEECCCCCEEEECEEE
FNHESPLRARDFVTRKITHAIAMIKLGKLDVLELGNLDAKRDWGFAKEYVEGMWRMLQAD
ECCCCCCHHHHHHHHHHHHHHHHHEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
EPDTFVLATNRTETVRDFVSLAASAVDIQLEWKGQAERECGVDVASGKTLVTINPKFYRP
CCCEEEEECCCHHHHHHHHHHHHHEEEEEEEECCCCCHHCCCEECCCCEEEEECCCCCCC
AEVDLLIGNPAKARTKLGWEPKTTLEELCHMMMEADVRRSERDFPF
CEEEEEECCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8548534; 10984043 [H]