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The map label for this gene is rfbD [H]

Identifier: 182682150

GI number: 182682150

Start: 1805670

End: 1806584

Strand: Reverse

Name: rfbD [H]

Synonym: XfasM23_1629

Alternate gene names: 182682150

Gene position: 1806584-1805670 (Counterclockwise)

Preceding gene: 182682151

Following gene: 182682149

Centisome position: 71.25

GC content: 48.42

Gene sequence:

>915_bases
ATGAATTTTTCTGGTAAGCGTGTTCTATTGACAGGTAGCAGCGGTTTTACCGGTCGATATATGCTGGACGAGCTTCTTAG
CCATGGTTACGAAGTCATTGCCCTTGGTAAGTCTCTCCCTGAGACACAAGATGATTCGGTGCGTTGGTTGCAAGCTGATC
TGTGTGATCTCGACGCGCTTACCCGGAGTGTGGCTGATGTGCGTCCGGATGTTGTGATCCATCTGGCTGCGCTGGCTTTT
GTTGCACATGGTAGTGCAAACGATTTCTACCACGTCAATGTGATCGGCACGCGTAACCTATTAACTGCTTTAACCCACGG
CGATGCAACGCCAGAGTGTGTGTTGCTGGCCAGCAGTGCCAACGTATATGGCAATGCATCCGAAGGGGAGTTGGAAGAGT
CTACGAAGCCAGCACCAACCAACGATTATGCAGTGAGCAAACTTTCTATGGAGTATGCCGCTAGCTTGTGGCGTGAGCGT
TTACCGTTGGTGGTTACCCGACCGTTTAATTACACAGGGATTGGGCAGGCAGATACTTTTCTTATCCCTAAAATCGTCGC
GCATTTTTGCCTTCGCTCCCAGCATATCGAACTTGGCAATCTTGATGTGCAGCGTGATTTTAGTGACGTGCGTTCAGTCG
TTCAGGCATATCGACGTTTGGTTGAAACCATAAACGCGGTTGGAAAAACAATCAACGTATGTTCTGGTGTTGCTTATTCC
CTGCGTGATGCGATTGATTTTTGCAGAGATATTACTGGACATGCCATTGAGGTTCGGATCAATCCGGCCTTTATGCGTAC
CAACGAGGTGCGTATTCTCCGTGGAAATCATTCTCTGTTGCGGCAATTGATTGGCCCTTGGAGTTCTCCTCCATTGAGAG
AAACATTGGAATGGATGCTCAAAACTGCTGTTTGA

Upstream 100 bases:

>100_bases
AACGAAGCTGGGTTGGGAACCAAAGACCACACTTGAGGAGCTTTGTCACATGATGATGGAAGCAGATGTGCGCCGCAGCG
AACGCGATTTCCCCTTCTAA

Downstream 100 bases:

>100_bases
TGGACGAGTCGACTGAGAAGTTTATCTTCCTTGGTCGATCAAGCTTGGATTTTGAAGTGTTCTTATGGAGTACCCATGTC
TGGAAATAACAACAGAGTGC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MNFSGKRVLLTGSSGFTGRYMLDELLSHGYEVIALGKSLPETQDDSVRWLQADLCDLDALTRSVADVRPDVVIHLAALAF
VAHGSANDFYHVNVIGTRNLLTALTHGDATPECVLLASSANVYGNASEGELEESTKPAPTNDYAVSKLSMEYAASLWRER
LPLVVTRPFNYTGIGQADTFLIPKIVAHFCLRSQHIELGNLDVQRDFSDVRSVVQAYRRLVETINAVGKTINVCSGVAYS
LRDAIDFCRDITGHAIEVRINPAFMRTNEVRILRGNHSLLRQLIGPWSSPPLRETLEWMLKTAV

Sequences:

>Translated_304_residues
MNFSGKRVLLTGSSGFTGRYMLDELLSHGYEVIALGKSLPETQDDSVRWLQADLCDLDALTRSVADVRPDVVIHLAALAF
VAHGSANDFYHVNVIGTRNLLTALTHGDATPECVLLASSANVYGNASEGELEESTKPAPTNDYAVSKLSMEYAASLWRER
LPLVVTRPFNYTGIGQADTFLIPKIVAHFCLRSQHIELGNLDVQRDFSDVRSVVQAYRRLVETINAVGKTINVCSGVAYS
LRDAIDFCRDITGHAIEVRINPAFMRTNEVRILRGNHSLLRQLIGPWSSPPLRETLEWMLKTAV
>Mature_304_residues
MNFSGKRVLLTGSSGFTGRYMLDELLSHGYEVIALGKSLPETQDDSVRWLQADLCDLDALTRSVADVRPDVVIHLAALAF
VAHGSANDFYHVNVIGTRNLLTALTHGDATPECVLLASSANVYGNASEGELEESTKPAPTNDYAVSKLSMEYAASLWRER
LPLVVTRPFNYTGIGQADTFLIPKIVAHFCLRSQHIELGNLDVQRDFSDVRSVVQAYRRLVETINAVGKTINVCSGVAYS
LRDAIDFCRDITGHAIEVRINPAFMRTNEVRILRGNHSLLRQLIGPWSSPPLRETLEWMLKTAV

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=321, Percent_Identity=27.4143302180685, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI1788366, Length=347, Percent_Identity=24.7838616714697, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI48994969, Length=308, Percent_Identity=25.974025974026, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788353, Length=261, Percent_Identity=27.5862068965517, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17507723, Length=327, Percent_Identity=26.9113149847095, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI133901786, Length=328, Percent_Identity=27.4390243902439, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI133901788, Length=328, Percent_Identity=27.4390243902439, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17539424, Length=328, Percent_Identity=27.4390243902439, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17539422, Length=332, Percent_Identity=27.4096385542169, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI133901790, Length=328, Percent_Identity=27.4390243902439, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71982035, Length=330, Percent_Identity=25.7575757575758, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71982038, Length=332, Percent_Identity=25.6024096385542, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=164, Percent_Identity=28.6585365853659, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24158427, Length=316, Percent_Identity=24.3670886075949, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 33615; Mature: 33615

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFSGKRVLLTGSSGFTGRYMLDELLSHGYEVIALGKSLPETQDDSVRWLQADLCDLDAL
CCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
TRSVADVRPDVVIHLAALAFVAHGSANDFYHVNVIGTRNLLTALTHGDATPECVLLASSA
HHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHHHHCCCCCCCEEEEEECC
NVYGNASEGELEESTKPAPTNDYAVSKLSMEYAASLWRERLPLVVTRPFNYTGIGQADTF
CCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHH
LIPKIVAHFCLRSQHIELGNLDVQRDFSDVRSVVQAYRRLVETINAVGKTINVCSGVAYS
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRDAIDFCRDITGHAIEVRINPAFMRTNEVRILRGNHSLLRQLIGPWSSPPLRETLEWML
HHHHHHHHHHCCCCEEEEEECHHEEECCCEEEEECCHHHHHHHHCCCCCCCHHHHHHHHH
KTAV
HHCC
>Mature Secondary Structure
MNFSGKRVLLTGSSGFTGRYMLDELLSHGYEVIALGKSLPETQDDSVRWLQADLCDLDAL
CCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
TRSVADVRPDVVIHLAALAFVAHGSANDFYHVNVIGTRNLLTALTHGDATPECVLLASSA
HHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHHHHCCCCCCCEEEEEECC
NVYGNASEGELEESTKPAPTNDYAVSKLSMEYAASLWRERLPLVVTRPFNYTGIGQADTF
CCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHH
LIPKIVAHFCLRSQHIELGNLDVQRDFSDVRSVVQAYRRLVETINAVGKTINVCSGVAYS
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRDAIDFCRDITGHAIEVRINPAFMRTNEVRILRGNHSLLRQLIGPWSSPPLRETLEWML
HHHHHHHHHHCCCCEEEEEECHHEEECCCEEEEECCHHHHHHHHCCCCCCCHHHHHHHHH
KTAV
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1372980; 7540582; 10952301 [H]