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The map label for this gene is tilS [H]

Identifier: 182682123

GI number: 182682123

Start: 1774763

End: 1776070

Strand: Reverse

Name: tilS [H]

Synonym: XfasM23_1601

Alternate gene names: 182682123

Gene position: 1776070-1774763 (Counterclockwise)

Preceding gene: 182682124

Following gene: 182682122

Centisome position: 70.04

GC content: 62.54

Gene sequence:

>1308_bases
GTGTCTTCTCCCTTTCCCCGCATTCCAGATCCTGGCGTTCCCGTCCTGGTTGCTTTCAGCGGCGGATTGGATTCAACCGT
CCTGCTGCATTGCTTAGCCAGCCAGCCAACCCAGCGCATCCACGGCCTGGAAGCCATCCACATCCATCATGGACTCAATG
AAAACGCCGATGCTTGGACCGCCCACTGCGCCGCCTTTTGCACACAACACGACATCCGCCTACACATCGCGCGTGTCCAC
GTTTCCCACAACAGCGGACAAGGCTTGGAAGCGGCCGCCCGCACCGCGCGCCGCGCCGCATTTGCCCACACCTTGCAACA
TGGCCACTACCTGGCATTGGCGCATCATTGCGATGACCAAGCCGAGACATGGTTGTTACGCGCACTACGTGGCTCCTGCG
ACGGACTCGCCGCCATGCGGCCATTGACCCCATTTGCCGCGGGCCACCTGTGGCGTCCCCTGCTGACCCACTCCCGGGCG
CAATTATTGGACTACGCACAACAGCAGCACCTGGATTGGATTGAAGATTCCAGTAACGCGGATCTGCGTCATGACCGCAA
CTTCCTGCGCATCCACGTATTGCCATTACTGCATCAGCGCTGGCCCCAGGCCACGGCTGTACTGGCCCGCAATGCGGCAC
TGGCAGCGGCCAACGCCGACCTCCTCAATGCTGAAGATGCCGTACTGCTGCCTGACCTACTTGACCCTGACGGCGCATTG
GACATCAACGCCCTCACTGCCCACCCACCCGCACGGCGCGCCCGACTGCTGCGCGCCTGGTGTGCCCGCGCAGGTGCTCC
CCCACTCCCTGAGCGCGGTGTGAACATTATCGAGCGCGAACTACTGCCTGCCAGACACGATTCCGCCGCCTGCTTCACCT
GGTCTCATACCGAGATCCGGCGCTGGCGCCTGCGCCTATACCTGCATCGTCCACAACCGCCATGGCCCCCTGACTGGCAG
CCCCTGTGGAGCGGCACCGCACCGCTGATCCTGCCTGATGGTGGCCAACTCCACCTAGAGAGCACCGATCACGAGACCGT
GCCCGGTTTTCCACACCCCCTGCGCGTGCGTGCCCGCCGTGGAGGAGAGCGCTTGATCTTGCCTGGGCGTACCCATTCCC
ACCCCCTCAAACACCTCTTGCAAGACGTCGGCATCCCCCCCTGGCGGCGTGCATCAATGCCCTTATTGTGCGACGGTGAA
CAGATCCTCGCCGTTGGCGATGCCCTACTGGCCGCACCACTGGTCACCTGGTTGCAGGCTCATCGCCTCAAACTACGCTG
GCAATACCACAACAACACATGCATTTGA

Upstream 100 bases:

>100_bases
GTGTATTTGAATACACCAGGAGACACACTCCCTACCGCCGCTGGCGGCTCGCTGAGTCAGCAGAGATACTGCATCCCCAT
TTCACCCCCCTGCATTGACC

Downstream 100 bases:

>100_bases
CCATCCCGATGACACCCCGCACACTTGCCCGATGCCAAAACGCTCCCCGCCAGACACCTCCCCCGTTGCCCGCTTTGAAC
AGTCTCTTCAAGAATTGGAG

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSSPFPRIPDPGVPVLVAFSGGLDSTVLLHCLASQPTQRIHGLEAIHIHHGLNENADAWTAHCAAFCTQHDIRLHIARVH
VSHNSGQGLEAAARTARRAAFAHTLQHGHYLALAHHCDDQAETWLLRALRGSCDGLAAMRPLTPFAAGHLWRPLLTHSRA
QLLDYAQQQHLDWIEDSSNADLRHDRNFLRIHVLPLLHQRWPQATAVLARNAALAAANADLLNAEDAVLLPDLLDPDGAL
DINALTAHPPARRARLLRAWCARAGAPPLPERGVNIIERELLPARHDSAACFTWSHTEIRRWRLRLYLHRPQPPWPPDWQ
PLWSGTAPLILPDGGQLHLESTDHETVPGFPHPLRVRARRGGERLILPGRTHSHPLKHLLQDVGIPPWRRASMPLLCDGE
QILAVGDALLAAPLVTWLQAHRLKLRWQYHNNTCI

Sequences:

>Translated_435_residues
MSSPFPRIPDPGVPVLVAFSGGLDSTVLLHCLASQPTQRIHGLEAIHIHHGLNENADAWTAHCAAFCTQHDIRLHIARVH
VSHNSGQGLEAAARTARRAAFAHTLQHGHYLALAHHCDDQAETWLLRALRGSCDGLAAMRPLTPFAAGHLWRPLLTHSRA
QLLDYAQQQHLDWIEDSSNADLRHDRNFLRIHVLPLLHQRWPQATAVLARNAALAAANADLLNAEDAVLLPDLLDPDGAL
DINALTAHPPARRARLLRAWCARAGAPPLPERGVNIIERELLPARHDSAACFTWSHTEIRRWRLRLYLHRPQPPWPPDWQ
PLWSGTAPLILPDGGQLHLESTDHETVPGFPHPLRVRARRGGERLILPGRTHSHPLKHLLQDVGIPPWRRASMPLLCDGE
QILAVGDALLAAPLVTWLQAHRLKLRWQYHNNTCI
>Mature_434_residues
SSPFPRIPDPGVPVLVAFSGGLDSTVLLHCLASQPTQRIHGLEAIHIHHGLNENADAWTAHCAAFCTQHDIRLHIARVHV
SHNSGQGLEAAARTARRAAFAHTLQHGHYLALAHHCDDQAETWLLRALRGSCDGLAAMRPLTPFAAGHLWRPLLTHSRAQ
LLDYAQQQHLDWIEDSSNADLRHDRNFLRIHVLPLLHQRWPQATAVLARNAALAAANADLLNAEDAVLLPDLLDPDGALD
INALTAHPPARRARLLRAWCARAGAPPLPERGVNIIERELLPARHDSAACFTWSHTEIRRWRLRLYLHRPQPPWPPDWQP
LWSGTAPLILPDGGQLHLESTDHETVPGFPHPLRVRARRGGERLILPGRTHSHPLKHLLQDVGIPPWRRASMPLLCDGEQ
ILAVGDALLAAPLVTWLQAHRLKLRWQYHNNTCI

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=400, Percent_Identity=41.75, Blast_Score=253, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 48564; Mature: 48433

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPFPRIPDPGVPVLVAFSGGLDSTVLLHCLASQPTQRIHGLEAIHIHHGLNENADAWT
CCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHCCCEEEEEECCCCCCCHHHH
AHCAAFCTQHDIRLHIARVHVSHNSGQGLEAAARTARRAAFAHTLQHGHYLALAHHCDDQ
HHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCH
AETWLLRALRGSCDGLAAMRPLTPFAAGHLWRPLLTHSRAQLLDYAQQQHLDWIEDSSNA
HHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCC
DLRHDRNFLRIHVLPLLHQRWPQATAVLARNAALAAANADLLNAEDAVLLPDLLDPDGAL
CCHHCCCEEEEEEEHHHHHCCCHHHHHHHHCHHHEECCCCCCCCCCCEECCCCCCCCCCE
DINALTAHPPARRARLLRAWCARAGAPPLPERGVNIIERELLPARHDSAACFTWSHTEIR
EEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHCCCCCCCCEEEEECHHHHH
RWRLRLYLHRPQPPWPPDWQPLWSGTAPLILPDGGQLHLESTDHETVPGFPHPLRVRARR
EEEEEEEEECCCCCCCCCCCHHHCCCCCEEECCCCEEEEECCCCCCCCCCCCCHHHHCCC
GGERLILPGRTHSHPLKHLLQDVGIPPWRRASMPLLCDGEQILAVGDALLAAPLVTWLQA
CCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHH
HRLKLRWQYHNNTCI
HCEEEEEEECCCCCC
>Mature Secondary Structure 
SSPFPRIPDPGVPVLVAFSGGLDSTVLLHCLASQPTQRIHGLEAIHIHHGLNENADAWT
CCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHCCCEEEEEECCCCCCCHHHH
AHCAAFCTQHDIRLHIARVHVSHNSGQGLEAAARTARRAAFAHTLQHGHYLALAHHCDDQ
HHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCH
AETWLLRALRGSCDGLAAMRPLTPFAAGHLWRPLLTHSRAQLLDYAQQQHLDWIEDSSNA
HHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCC
DLRHDRNFLRIHVLPLLHQRWPQATAVLARNAALAAANADLLNAEDAVLLPDLLDPDGAL
CCHHCCCEEEEEEEHHHHHCCCHHHHHHHHCHHHEECCCCCCCCCCCEECCCCCCCCCCE
DINALTAHPPARRARLLRAWCARAGAPPLPERGVNIIERELLPARHDSAACFTWSHTEIR
EEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHCCCCCCCCEEEEECHHHHH
RWRLRLYLHRPQPPWPPDWQPLWSGTAPLILPDGGQLHLESTDHETVPGFPHPLRVRARR
EEEEEEEEECCCCCCCCCCCHHHCCCCCEEECCCCEEEEECCCCCCCCCCCCCHHHHCCC
GGERLILPGRTHSHPLKHLLQDVGIPPWRRASMPLLCDGEQILAVGDALLAAPLVTWLQA
CCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHH
HRLKLRWQYHNNTCI
HCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]