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The map label for this gene is bioD

Identifier: 182682102

GI number: 182682102

Start: 1752301

End: 1753113

Strand: Direct

Name: bioD

Synonym: XfasM23_1580

Alternate gene names: 182682102

Gene position: 1752301-1753113 (Clockwise)

Preceding gene: 182682101

Following gene: 182682103

Centisome position: 69.11

GC content: 57.69

Gene sequence:

>813_bases
ATGAAAGATCGTATCGGTGACACAGTCCCTGTTGCGGGGGCAACGTCCTACTGCAAGCTACAGACAGGGGCTAGGGATAA
ACAGGAACTGCGGATATGCTGGGAGGCTTTTCCATTTCACTCGGCACAGTGCATGTCCTTGTCTGATTTCTATGTCACCG
GCACCGATACTGGTATCGGAAAAACCTTTGTCAGTTGTATTTTGCTGCATATGCTGCGCGGGCGCGGGCAACGCGCGGTA
GGTATGAAGCCCGTCGCCAGCGGCTGTACGTATAGCGATACTGGGTGGCGGAACGAAGATGCTCTAGCACTGCAAGCAGC
CAGCGATCCAACGCCTGCCTACGATCTGATTAATCCCTACGCACTTCCCGCCGCAGTGGCACCCGAGATCGCGGCAATCG
AGGCTGGGGTCACTGTGGCGCTAGAACCGTTGAGTGCCTCATTCACTCAGTTACGCGCACAGGCGGATGTGGTGGTGGTC
GAGGGAGTGGGTGGCTGGGCTACACCGCTAAACGCCACCTTTGATCAAGCCACCTTGGTACGCGCTTTAGAGATACCCGT
CGTGCTGGTCGTCGGGCTGCGTCTGGGCTGTATGAATCACGCGCGCCTGAGTACAGCGGCGATCATGGCTGACGGGCTGC
GCTGTATCGGCTGGATCGCTAACACCATTGACCCACACATGGCACGTATTGAAGAGAATCTGGCTCTGTTACGCCAACGC
CTGCCAATCCCCTACTGGGGGCACCTACCGCATATCCCCCCAGGGATAAACCCGGCGACATTGGCCACACGGCTACATCC
ACAGGGGGATTGA

Upstream 100 bases:

>100_bases
CACGCGCTGCAACAAGTCATTGTCAGCAGTGGTTACGGGCGGGAGTTCGATCCGAAGCGCTGAGTCGGTATGGTTCTCTT
GGAACGTTTGTAAGGTTGGG

Downstream 100 bases:

>100_bases
ACCATTGGCATGCTGTCATGCTAAAGCAGTGTTGCGGGTTGAATCCAAATAGGGATCACGTTGCGATTCGGAAGATCTTG
ATGCCAACACAGCTAAAAGA

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MKDRIGDTVPVAGATSYCKLQTGARDKQELRICWEAFPFHSAQCMSLSDFYVTGTDTGIGKTFVSCILLHMLRGRGQRAV
GMKPVASGCTYSDTGWRNEDALALQAASDPTPAYDLINPYALPAAVAPEIAAIEAGVTVALEPLSASFTQLRAQADVVVV
EGVGGWATPLNATFDQATLVRALEIPVVLVVGLRLGCMNHARLSTAAIMADGLRCIGWIANTIDPHMARIEENLALLRQR
LPIPYWGHLPHIPPGINPATLATRLHPQGD

Sequences:

>Translated_270_residues
MKDRIGDTVPVAGATSYCKLQTGARDKQELRICWEAFPFHSAQCMSLSDFYVTGTDTGIGKTFVSCILLHMLRGRGQRAV
GMKPVASGCTYSDTGWRNEDALALQAASDPTPAYDLINPYALPAAVAPEIAAIEAGVTVALEPLSASFTQLRAQADVVVV
EGVGGWATPLNATFDQATLVRALEIPVVLVVGLRLGCMNHARLSTAAIMADGLRCIGWIANTIDPHMARIEENLALLRQR
LPIPYWGHLPHIPPGINPATLATRLHPQGD
>Mature_270_residues
MKDRIGDTVPVAGATSYCKLQTGARDKQELRICWEAFPFHSAQCMSLSDFYVTGTDTGIGKTFVSCILLHMLRGRGQRAV
GMKPVASGCTYSDTGWRNEDALALQAASDPTPAYDLINPYALPAAVAPEIAAIEAGVTVALEPLSASFTQLRAQADVVVV
EGVGGWATPLNATFDQATLVRALEIPVVLVVGLRLGCMNHARLSTAAIMADGLRCIGWIANTIDPHMARIEENLALLRQR
LPIPYWGHLPHIPPGINPATLATRLHPQGD

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=214, Percent_Identity=42.5233644859813, Blast_Score=164, Evalue=4e-42,
Organism=Escherichia coli, GI87081944, Length=207, Percent_Identity=40.0966183574879, Blast_Score=153, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 28935; Mature: 28935

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDRIGDTVPVAGATSYCKLQTGARDKQELRICWEAFPFHSAQCMSLSDFYVTGTDTGIG
CCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCEEEECCCCCHH
KTFVSCILLHMLRGRGQRAVGMKPVASGCTYSDTGWRNEDALALQAASDPTPAYDLINPY
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHCCCC
ALPAAVAPEIAAIEAGVTVALEPLSASFTQLRAQADVVVVEGVGGWATPLNATFDQATLV
CCCHHHCCCHHHHHCCCEEEEECCHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHH
RALEIPVVLVVGLRLGCMNHARLSTAAIMADGLRCIGWIANTIDPHMARIEENLALLRQR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LPIPYWGHLPHIPPGINPATLATRLHPQGD
CCCCCCCCCCCCCCCCCHHHHHEECCCCCC
>Mature Secondary Structure
MKDRIGDTVPVAGATSYCKLQTGARDKQELRICWEAFPFHSAQCMSLSDFYVTGTDTGIG
CCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCEEEECCCCCHH
KTFVSCILLHMLRGRGQRAVGMKPVASGCTYSDTGWRNEDALALQAASDPTPAYDLINPY
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHCCCC
ALPAAVAPEIAAIEAGVTVALEPLSASFTQLRAQADVVVVEGVGGWATPLNATFDQATLV
CCCHHHCCCHHHHHCCCEEEEECCHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHH
RALEIPVVLVVGLRLGCMNHARLSTAAIMADGLRCIGWIANTIDPHMARIEENLALLRQR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LPIPYWGHLPHIPPGINPATLATRLHPQGD
CCCCCCCCCCCCCCCCCHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]