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The map label for this gene is rluB [H]

Identifier: 182682078

GI number: 182682078

Start: 1729716

End: 1731146

Strand: Direct

Name: rluB [H]

Synonym: XfasM23_1556

Alternate gene names: 182682078

Gene position: 1729716-1731146 (Clockwise)

Preceding gene: 182682077

Following gene: 182682082

Centisome position: 68.21

GC content: 55.21

Gene sequence:

>1431_bases
ATGAGTGATACCCCAAAACCACCATCAAACACACTTTCACTCAAACGCGAGACAGCCACAGAAGCACCAAAACTGGAAGA
GCGCCTGCATAAAGTGCTTGCCCAAGCGGGTCTAGGTTCCCGCCGTGCCCTGGAACAACGCATCTCCAACGGACTCATCA
AAGTCAATGGAGACATTGCGCAGCTCGGCATGTCCGTTAAAAGTGGCGACAAAATCGAACTGGACGGCCGCAGCTTCGTC
GCCAGCGCCCTCACTGAACCGGCGCGCGTACTGATCTACAACAAACCAGAAGGCGAAGTGACCACACGCGAAGACCCAGA
GGGTCGCCCCACTGTGTTCGAAACACTCCCCGTCCTCAAAGGCGCACGTTGGATCGCCATCGGCCGCCTGGATATCAACA
CCACTGGCTTATTACTGCTCACCACAGACGGCGAACTTGCCAATGCAATGATGCACCCCTCATCGGAAATAGAACGCGAA
TATGTCGTACGTGTACGCTCCCCCGAAGGAGAAGAACATGTGCAGGATGAGCTACTCGAGCAACTTACGCGCGGCGTCAT
GCTGGAAGACGGCACCGCAAAATTCGACACGATTGAACGCATCGGCAACACCGACTCACACGACTGGTTTCGTGTCGTCG
TCAAAGAAGGCCGCAACCGCGAAGTACGTCGGCTATGGGAATCGCAAGGCTGCCAAGTCAGCCGCCTCAAACGTACACGT
TATGGTTCCGTCCTCCTACCACGCGAACTACTGCGTGGCCAATCGACCGAACTACCCAAGACCCAAGTAGAAGCACTACG
CACCCAACTGAAACTAGAAAAAGACATGCCGTTAGCACTCACCCTGCAACCGGTTATCGGCCAACGCCGTTCAGCCAAAG
CCACGCTGCACGTGAATCGCAACGACAACAACAAACACGCCTACCACAACAACCACAGCACCGCCGATGAAAGTCGCGAA
CTGCGTCGCTTTGACACCCTGCGTGATGACCGAGGTCGCGGCCAAGGCAAGCACCACTTCAAAGATAGACTGACTGTCAG
TGGTGAAGCTGCCGCAAAACAAGCACACAAACCCTTCAAACAATACAAACCCAAGAACGACCGTTCGCTATCGGAAGGCA
GCCCGGCAACATTCCAGAGCTGGTACGTCCCTGAAGGCGTCAGCACCGGCCCCCGCAACCACCGCAATGCTGGTGCTGGC
AATGGTGCCCACCCCAACAAAAAAAGCCCAAACCCAAACACCCGTAACACACAGGGACAACAAACACGCAAGTCTCCATA
CAAATACACGAACAATGCACCCAACTTCCCCTCCGACCACGCCACGCCAACCTTCAACCCCTATGGCAACCCTGGTCAGA
AAACAGGAGCAGGCCAACCAAACAACAGCGGTGGCAAATACAACCGGAATCGGGGACCAAGATACCCATAA

Upstream 100 bases:

>100_bases
CACGAAGACAACACCATCAACAAAACGTCTACAGCCGATCACTACATTGACTCAGACTCACAGAGCGACGCAGACACGCC
CCCGGACGGAACTTACCCCC

Downstream 100 bases:

>100_bases
TCATTCAACCCTCATTAGCCCCATTGAAACATCTCAAGCATTCACCACCACATTCCCCCCTTACAAACGCTTGAAACCAA
ATCAATCACCAACGAATAAC

Product: RNA-binding S4 domain-containing protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSDTPKPPSNTLSLKRETATEAPKLEERLHKVLAQAGLGSRRALEQRISNGLIKVNGDIAQLGMSVKSGDKIELDGRSFV
ASALTEPARVLIYNKPEGEVTTREDPEGRPTVFETLPVLKGARWIAIGRLDINTTGLLLLTTDGELANAMMHPSSEIERE
YVVRVRSPEGEEHVQDELLEQLTRGVMLEDGTAKFDTIERIGNTDSHDWFRVVVKEGRNREVRRLWESQGCQVSRLKRTR
YGSVLLPRELLRGQSTELPKTQVEALRTQLKLEKDMPLALTLQPVIGQRRSAKATLHVNRNDNNKHAYHNNHSTADESRE
LRRFDTLRDDRGRGQGKHHFKDRLTVSGEAAAKQAHKPFKQYKPKNDRSLSEGSPATFQSWYVPEGVSTGPRNHRNAGAG
NGAHPNKKSPNPNTRNTQGQQTRKSPYKYTNNAPNFPSDHATPTFNPYGNPGQKTGAGQPNNSGGKYNRNRGPRYP

Sequences:

>Translated_476_residues
MSDTPKPPSNTLSLKRETATEAPKLEERLHKVLAQAGLGSRRALEQRISNGLIKVNGDIAQLGMSVKSGDKIELDGRSFV
ASALTEPARVLIYNKPEGEVTTREDPEGRPTVFETLPVLKGARWIAIGRLDINTTGLLLLTTDGELANAMMHPSSEIERE
YVVRVRSPEGEEHVQDELLEQLTRGVMLEDGTAKFDTIERIGNTDSHDWFRVVVKEGRNREVRRLWESQGCQVSRLKRTR
YGSVLLPRELLRGQSTELPKTQVEALRTQLKLEKDMPLALTLQPVIGQRRSAKATLHVNRNDNNKHAYHNNHSTADESRE
LRRFDTLRDDRGRGQGKHHFKDRLTVSGEAAAKQAHKPFKQYKPKNDRSLSEGSPATFQSWYVPEGVSTGPRNHRNAGAG
NGAHPNKKSPNPNTRNTQGQQTRKSPYKYTNNAPNFPSDHATPTFNPYGNPGQKTGAGQPNNSGGKYNRNRGPRYP
>Mature_475_residues
SDTPKPPSNTLSLKRETATEAPKLEERLHKVLAQAGLGSRRALEQRISNGLIKVNGDIAQLGMSVKSGDKIELDGRSFVA
SALTEPARVLIYNKPEGEVTTREDPEGRPTVFETLPVLKGARWIAIGRLDINTTGLLLLTTDGELANAMMHPSSEIEREY
VVRVRSPEGEEHVQDELLEQLTRGVMLEDGTAKFDTIERIGNTDSHDWFRVVVKEGRNREVRRLWESQGCQVSRLKRTRY
GSVLLPRELLRGQSTELPKTQVEALRTQLKLEKDMPLALTLQPVIGQRRSAKATLHVNRNDNNKHAYHNNHSTADESREL
RRFDTLRDDRGRGQGKHHFKDRLTVSGEAAAKQAHKPFKQYKPKNDRSLSEGSPATFQSWYVPEGVSTGPRNHRNAGAGN
GAHPNKKSPNPNTRNTQGQQTRKSPYKYTNNAPNFPSDHATPTFNPYGNPGQKTGAGQPNNSGGKYNRNRGPRYP

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=251, Percent_Identity=55.3784860557769, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1790453, Length=224, Percent_Identity=31.25, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788510, Length=243, Percent_Identity=31.6872427983539, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87081838, Length=176, Percent_Identity=31.25, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 53211; Mature: 53080

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTPKPPSNTLSLKRETATEAPKLEERLHKVLAQAGLGSRRALEQRISNGLIKVNGDIA
CCCCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEECCHHH
QLGMSVKSGDKIELDGRSFVASALTEPARVLIYNKPEGEVTTREDPEGRPTVFETLPVLK
HHCCCCCCCCEEEECCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHC
GARWIAIGRLDINTTGLLLLTTDGELANAMMHPSSEIEREYVVRVRSPEGEEHVQDELLE
CCEEEEEEEEEECCCEEEEEECCCHHHHHHCCCHHHHCCEEEEEECCCCCHHHHHHHHHH
QLTRGVMLEDGTAKFDTIERIGNTDSHDWFRVVVKEGRNREVRRLWESQGCQVSRLKRTR
HHHCCEEECCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHH
YGSVLLPRELLRGQSTELPKTQVEALRTQLKLEKDMPLALTLQPVIGQRRSAKATLHVNR
CCCEECCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHCCCCCCEEEEEEEC
NDNNKHAYHNNHSTADESRELRRFDTLRDDRGRGQGKHHFKDRLTVSGEAAAKQAHKPFK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCEEECCHHHHHHHHCCHH
QYKPKNDRSLSEGSPATFQSWYVPEGVSTGPRNHRNAGAGNGAHPNKKSPNPNTRNTQGQ
HCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
QTRKSPYKYTNNAPNFPSDHATPTFNPYGNPGQKTGAGQPNNSGGKYNRNRGPRYP
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDTPKPPSNTLSLKRETATEAPKLEERLHKVLAQAGLGSRRALEQRISNGLIKVNGDIA
CCCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEECCHHH
QLGMSVKSGDKIELDGRSFVASALTEPARVLIYNKPEGEVTTREDPEGRPTVFETLPVLK
HHCCCCCCCCEEEECCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHC
GARWIAIGRLDINTTGLLLLTTDGELANAMMHPSSEIEREYVVRVRSPEGEEHVQDELLE
CCEEEEEEEEEECCCEEEEEECCCHHHHHHCCCHHHHCCEEEEEECCCCCHHHHHHHHHH
QLTRGVMLEDGTAKFDTIERIGNTDSHDWFRVVVKEGRNREVRRLWESQGCQVSRLKRTR
HHHCCEEECCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHH
YGSVLLPRELLRGQSTELPKTQVEALRTQLKLEKDMPLALTLQPVIGQRRSAKATLHVNR
CCCEECCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHCCCCCCEEEEEEEC
NDNNKHAYHNNHSTADESRELRRFDTLRDDRGRGQGKHHFKDRLTVSGEAAAKQAHKPFK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCEEECCHHHHHHHHCCHH
QYKPKNDRSLSEGSPATFQSWYVPEGVSTGPRNHRNAGAGNGAHPNKKSPNPNTRNTQGQ
HCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
QTRKSPYKYTNNAPNFPSDHATPTFNPYGNPGQKTGAGQPNNSGGKYNRNRGPRYP
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]