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The map label for this gene is queF

Identifier: 182682012

GI number: 182682012

Start: 1642989

End: 1643816

Strand: Reverse

Name: queF

Synonym: XfasM23_1487

Alternate gene names: 182682012

Gene position: 1643816-1642989 (Counterclockwise)

Preceding gene: 182682016

Following gene: 182682010

Centisome position: 64.83

GC content: 50.72

Gene sequence:

>828_bases
ATGAACACCTCTCACTATTCTGTTCTTGGACATACGGTTCCCTACCCAAAAGTTTACGATCCGTCGTTACTTTTCCCAAT
TTCCCGTGCTGTAGGGCGTACTCAAATCGGTATCGGTGTGGTGTTGCCGTTCGTTGGTGAGGATCGTTGGCATGCTTATG
AGCTGAGTTGGTTGGATGCACGCGGTAAGCCATGTGTGGCTACAGCGACGTTTCATGTGCCATGTGATTCGCCCTATTTG
ATTGAATCCAAGTCGCTCAAACTCTATCTCAATTCGTTCAGTGCTGAGGTGTTTAATCGTGCTGAGGCGCTGCGCTTACG
CATCGCCGCTGATCTGTCGGCGTGCGCTGGTGCGGCAGTTGCGGTTGAATTTGGGTTGCCGCCGGTTGGGAGCGGGGACA
AGGAAATATCGCTTGATAGGTTGAATGTGGACATTGAGGACTACGGTCCACCGAATCCCGATTATTTGTCCAATGTTGCT
CAGAATTTGGTTGAAGAAATGGTTGAAGAAACTCTAACTTCGACCTTATTCAAGTCTAATTGCCCGGTGACTGGTCAACC
AGACTGGGCCAGCGTCACGGTGCGTTACTTTGGTATGCCGATTGATCATGAGGGCTTGTTACGTTACTTCATCAGTTTTC
GTCATCATGCTGAATTTCATGAGCAATGCGTGGAGCGTATTTTTCAGGATGTGTTACAGCGATGCGCCCCGCAATGCCTC
GCTGTAGAGGCACGTTATACCCGCCGCGGTGGCCTGGATATTAATCCTCTGCGTACCACGTCGGAGATGGCATGGCCGCT
CTCGGTCTTTCGTGATCCGCGTCAATAG

Upstream 100 bases:

>100_bases
TGGTGAACGGAATATGAATTGGAGAGGTGTGCATAGTTTTGATATTTCGACCCTAAATATAGTCCTCTTCAATCTTCACA
GCAGCTTCTAGAATCCAGGT

Downstream 100 bases:

>100_bases
TGCGTTGTTGCTGTGAATGTGGTGTTGTGCTCAGGGCTTGAAGAAGTGAACGTGCGGCAGTGCCATTTTGCTAGCAAAAT
GGAGTGAAGGTGATGCACAC

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MNTSHYSVLGHTVPYPKVYDPSLLFPISRAVGRTQIGIGVVLPFVGEDRWHAYELSWLDARGKPCVATATFHVPCDSPYL
IESKSLKLYLNSFSAEVFNRAEALRLRIAADLSACAGAAVAVEFGLPPVGSGDKEISLDRLNVDIEDYGPPNPDYLSNVA
QNLVEEMVEETLTSTLFKSNCPVTGQPDWASVTVRYFGMPIDHEGLLRYFISFRHHAEFHEQCVERIFQDVLQRCAPQCL
AVEARYTRRGGLDINPLRTTSEMAWPLSVFRDPRQ

Sequences:

>Translated_275_residues
MNTSHYSVLGHTVPYPKVYDPSLLFPISRAVGRTQIGIGVVLPFVGEDRWHAYELSWLDARGKPCVATATFHVPCDSPYL
IESKSLKLYLNSFSAEVFNRAEALRLRIAADLSACAGAAVAVEFGLPPVGSGDKEISLDRLNVDIEDYGPPNPDYLSNVA
QNLVEEMVEETLTSTLFKSNCPVTGQPDWASVTVRYFGMPIDHEGLLRYFISFRHHAEFHEQCVERIFQDVLQRCAPQCL
AVEARYTRRGGLDINPLRTTSEMAWPLSVFRDPRQ
>Mature_275_residues
MNTSHYSVLGHTVPYPKVYDPSLLFPISRAVGRTQIGIGVVLPFVGEDRWHAYELSWLDARGKPCVATATFHVPCDSPYL
IESKSLKLYLNSFSAEVFNRAEALRLRIAADLSACAGAAVAVEFGLPPVGSGDKEISLDRLNVDIEDYGPPNPDYLSNVA
QNLVEEMVEETLTSTLFKSNCPVTGQPDWASVTVRYFGMPIDHEGLLRYFISFRHHAEFHEQCVERIFQDVLQRCAPQCL
AVEARYTRRGGLDINPLRTTSEMAWPLSVFRDPRQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=264, Percent_Identity=47.3484848484849, Blast_Score=229, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_XYLF2 (B2I6I8)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830172.1
- ProteinModelPortal:   B2I6I8
- GeneID:   6202497
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1487
- HOGENOM:   HBG289139
- OMA:   PFRSNFE
- ProtClustDB:   PRK11792
- GO:   GO:0005737
- HAMAP:   MF_00817
- InterPro:   IPR016428
- InterPro:   IPR020602
- PIRSF:   PIRSF004750
- TIGRFAMs:   TIGR03138

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 30808; Mature: 30808

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSHYSVLGHTVPYPKVYDPSLLFPISRAVGRTQIGIGVVLPFVGEDRWHAYELSWLDA
CCCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCEECCEEEEEECCCCCEEEEEEEEECC
RGKPCVATATFHVPCDSPYLIESKSLKLYLNSFSAEVFNRAEALRLRIAADLSACAGAAV
CCCCEEEEEEEECCCCCCEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
AVEFGLPPVGSGDKEISLDRLNVDIEDYGPPNPDYLSNVAQNLVEEMVEETLTSTLFKSN
EEEECCCCCCCCCCEEEEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
CPVTGQPDWASVTVRYFGMPIDHEGLLRYFISFRHHAEFHEQCVERIFQDVLQRCAPQCL
CCCCCCCCHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHE
AVEARYTRRGGLDINPLRTTSEMAWPLSVFRDPRQ
EEHHHHHHCCCCCCCCCCCCHHHHCCHHHHCCCCC
>Mature Secondary Structure
MNTSHYSVLGHTVPYPKVYDPSLLFPISRAVGRTQIGIGVVLPFVGEDRWHAYELSWLDA
CCCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCEECCEEEEEECCCCCEEEEEEEEECC
RGKPCVATATFHVPCDSPYLIESKSLKLYLNSFSAEVFNRAEALRLRIAADLSACAGAAV
CCCCEEEEEEEECCCCCCEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
AVEFGLPPVGSGDKEISLDRLNVDIEDYGPPNPDYLSNVAQNLVEEMVEETLTSTLFKSN
EEEECCCCCCCCCCEEEEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
CPVTGQPDWASVTVRYFGMPIDHEGLLRYFISFRHHAEFHEQCVERIFQDVLQRCAPQCL
CCCCCCCCHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHE
AVEARYTRRGGLDINPLRTTSEMAWPLSVFRDPRQ
EEHHHHHHCCCCCCCCCCCCHHHHCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA