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The map label for this gene is leuC

Identifier: 182682010

GI number: 182682010

Start: 1638987

End: 1640411

Strand: Reverse

Name: leuC

Synonym: XfasM23_1485

Alternate gene names: 182682010

Gene position: 1640411-1638987 (Counterclockwise)

Preceding gene: 182682012

Following gene: 182682009

Centisome position: 64.69

GC content: 52.56

Gene sequence:

>1425_bases
ATGGCCGGTAAGACCCTGTATGGCAAATTGTGGGACATTCATGAGGTGGCCCGTCGCGACGATGGGTCTTCGCTCATTTA
CATTGATCGCCATATACTTCACGAAGTGACTTCACCACAGGCTTTTGAAGGATTGCGTTTAGCTGGACGGCCGTTGTGGC
GAGTCAATGCCAATATTGCGACTCCGGATCATAATGTCCCGACGACCAAGGCGGAGCGTCAAGGCAGTTTGCTATCGATT
GCCGATACGGTTTCGCGTTTGCAGGTACAGACGTTGGATGAAAATTGCGACGATTTCGGTATCTTCGAATTCAAGATGAA
TGATGTGCGTCAAGGGATCGTCCACGTGATCGGTCCAGAACAGGGAGCCACCTTGCCTGGTATGACAGTAGTGTGCGGTG
ATTCACACACCTCCACACACGGTGCCTTTGGTGCATTGGCGCATGGCATTGGTACCTCTGAAGTTGAGCACGTGTTAGCC
ACCCAGTGCTTAGTCACCCAGAAGATGAAAAATATGCAAGTGCGTGTGGAAGGCACCTTGCCATGGGGGGTGACGGCCAA
AGATATCGTATTGGCGTTGATTGGGAAGATAGGCACTGCCGGAGGCAACGGTTATGCGGTGGAGTTTTCAGGGAGTACCA
TCCGCGCGTTGTCGATGGAAGGGCGCATGACCATTTGTAATATGGCTATTGAGGCTGGTGCGCGCGTGGGTATGGTGGCG
GTGGACGAGAAGACCATCCAGTATGTGCATGGCCGCCCGTTTGCACCTAAAGGATCCGATTGGGATGCTGCTGTTGCATT
CTGGCGTGGTTTGGTTTCCGATCCAGATGCGCACTTTGATCGTGTGGTTGAGTTATCTGCTGAAGAGATTAAACCTCAGG
TTACTTGGGGAACCTCGCCAGAGATGGTGTCGGCAGTGGATCAATCGGTGCCAGACCCAGAGCGCGAAACTGATCCAGTC
AAGAAGGAGTCTTTAATTCGTGCCCTGAAATATATGGGTTTGCAGCCGAATGATCCGATCACTTCAATAAAGCTGGACCG
TGTATTTATTGGCTCCTGTACGAATTCGCGTATTGAAGACTTACGTGCTGCTGCGGAAGTTGTTAAAGGCCGTAAAGTTG
CTTCCACGGTGAAGCAGGCGATGGTGGTGCCTGGCTCGGGATTGGTAAAGGCACAGGCTGAAGTTGAGGGCTTGGACAAG
ATATTCATTGAGGCTGGTTTCGAGTGGCGTGAACCTGGATGTTCGATGTGTTTGGCGATGAATCCCGATAAATTGGGCAG
TGGCGAGCATTGCGCATCTACTTCTAATCGTAACTTCGAGGGGCGCCAGGGCATCGGTGGTCGTACCCATTTAGTCAGCC
CTGCGATGGCAGCCGCTGCGGCCGTGGCTGGTCACTTTGTCGATGTCCGCGAAATGATGCGGTGA

Upstream 100 bases:

>100_bases
GAATTCCCATGATTGATCTTGATCAGCTCGCTCTAGTATGGTTGCCAACCAACGGTGACATCAGTCGCCAATTGTTGTAA
CCACATTAGGAATTGCTGCT

Downstream 100 bases:

>100_bases
TGAACCTTATGTTGTCTTTGTAGATTCTTGGAAATAACGTGCTAATGAAGGTTTATATTCAATGCCGTTTTTTGATTGTT
TCAATACTTCTAGCTCATCC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MAGKTLYGKLWDIHEVARRDDGSSLIYIDRHILHEVTSPQAFEGLRLAGRPLWRVNANIATPDHNVPTTKAERQGSLLSI
ADTVSRLQVQTLDENCDDFGIFEFKMNDVRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLA
TQCLVTQKMKNMQVRVEGTLPWGVTAKDIVLALIGKIGTAGGNGYAVEFSGSTIRALSMEGRMTICNMAIEAGARVGMVA
VDEKTIQYVHGRPFAPKGSDWDAAVAFWRGLVSDPDAHFDRVVELSAEEIKPQVTWGTSPEMVSAVDQSVPDPERETDPV
KKESLIRALKYMGLQPNDPITSIKLDRVFIGSCTNSRIEDLRAAAEVVKGRKVASTVKQAMVVPGSGLVKAQAEVEGLDK
IFIEAGFEWREPGCSMCLAMNPDKLGSGEHCASTSNRNFEGRQGIGGRTHLVSPAMAAAAAVAGHFVDVREMMR

Sequences:

>Translated_474_residues
MAGKTLYGKLWDIHEVARRDDGSSLIYIDRHILHEVTSPQAFEGLRLAGRPLWRVNANIATPDHNVPTTKAERQGSLLSI
ADTVSRLQVQTLDENCDDFGIFEFKMNDVRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLA
TQCLVTQKMKNMQVRVEGTLPWGVTAKDIVLALIGKIGTAGGNGYAVEFSGSTIRALSMEGRMTICNMAIEAGARVGMVA
VDEKTIQYVHGRPFAPKGSDWDAAVAFWRGLVSDPDAHFDRVVELSAEEIKPQVTWGTSPEMVSAVDQSVPDPERETDPV
KKESLIRALKYMGLQPNDPITSIKLDRVFIGSCTNSRIEDLRAAAEVVKGRKVASTVKQAMVVPGSGLVKAQAEVEGLDK
IFIEAGFEWREPGCSMCLAMNPDKLGSGEHCASTSNRNFEGRQGIGGRTHLVSPAMAAAAAVAGHFVDVREMMR
>Mature_473_residues
AGKTLYGKLWDIHEVARRDDGSSLIYIDRHILHEVTSPQAFEGLRLAGRPLWRVNANIATPDHNVPTTKAERQGSLLSIA
DTVSRLQVQTLDENCDDFGIFEFKMNDVRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLAT
QCLVTQKMKNMQVRVEGTLPWGVTAKDIVLALIGKIGTAGGNGYAVEFSGSTIRALSMEGRMTICNMAIEAGARVGMVAV
DEKTIQYVHGRPFAPKGSDWDAAVAFWRGLVSDPDAHFDRVVELSAEEIKPQVTWGTSPEMVSAVDQSVPDPERETDPVK
KESLIRALKYMGLQPNDPITSIKLDRVFIGSCTNSRIEDLRAAAEVVKGRKVASTVKQAMVVPGSGLVKAQAEVEGLDKI
FIEAGFEWREPGCSMCLAMNPDKLGSGEHCASTSNRNFEGRQGIGGRTHLVSPAMAAAAAVAGHFVDVREMMR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=363, Percent_Identity=29.4765840220386, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI8659555, Length=440, Percent_Identity=26.5909090909091, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI41352693, Length=367, Percent_Identity=26.158038147139, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1786259, Length=469, Percent_Identity=64.3923240938166, Blast_Score=600, Evalue=1e-173,
Organism=Escherichia coli, GI1787531, Length=366, Percent_Identity=26.2295081967213, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87081781, Length=362, Percent_Identity=24.8618784530387, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI2367097, Length=342, Percent_Identity=24.2690058479532, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=478, Percent_Identity=26.1506276150628, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI32564738, Length=365, Percent_Identity=27.3972602739726, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI25149342, Length=307, Percent_Identity=26.3843648208469, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17568399, Length=414, Percent_Identity=28.2608695652174, Blast_Score=101, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=470, Percent_Identity=63.4042553191489, Blast_Score=604, Evalue=1e-174,
Organism=Saccharomyces cerevisiae, GI6323335, Length=362, Percent_Identity=30.1104972375691, Blast_Score=133, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6320440, Length=354, Percent_Identity=29.6610169491525, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6322261, Length=482, Percent_Identity=27.5933609958506, Blast_Score=126, Evalue=6e-30,
Organism=Drosophila melanogaster, GI281365315, Length=400, Percent_Identity=25.25, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17864292, Length=400, Percent_Identity=25.25, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161076999, Length=400, Percent_Identity=25.25, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI28571643, Length=363, Percent_Identity=26.7217630853994, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI17137564, Length=383, Percent_Identity=27.4151436031332, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24645686, Length=373, Percent_Identity=28.686327077748, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_XYLF2 (B2I6I6)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830170.1
- ProteinModelPortal:   B2I6I6
- SMR:   B2I6I6
- GeneID:   6202494
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1485
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 51154; Mature: 51023

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGKTLYGKLWDIHEVARRDDGSSLIYIDRHILHEVTSPQAFEGLRLAGRPLWRVNANIA
CCCCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCCHHHCCHHHCCCCEEEEECEEE
TPDHNVPTTKAERQGSLLSIADTVSRLQVQTLDENCDDFGIFEFKMNDVRQGIVHVIGPE
CCCCCCCCCCHHHCCCEEHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHCCCEEEECCC
QGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQCLVTQKMKNMQVRVEGTL
CCCCCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECC
PWGVTAKDIVLALIGKIGTAGGNGYAVEFSGSTIRALSMEGRMTICNMAIEAGARVGMVA
CCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCCEEEEHHHHHCCCCEEEEE
VDEKTIQYVHGRPFAPKGSDWDAAVAFWRGLVSDPDAHFDRVVELSAEEIKPQVTWGTSP
ECHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCCCH
EMVSAVDQSVPDPERETDPVKKESLIRALKYMGLQPNDPITSIKLDRVFIGSCTNSRIED
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCHHHHH
LRAAAEVVKGRKVASTVKQAMVVPGSGLVKAQAEVEGLDKIFIEAGFEWREPGCSMCLAM
HHHHHHHHHCHHHHHHHHHHEECCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
NPDKLGSGEHCASTSNRNFEGRQGIGGRTHLVSPAMAAAAAVAGHFVDVREMMR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
AGKTLYGKLWDIHEVARRDDGSSLIYIDRHILHEVTSPQAFEGLRLAGRPLWRVNANIA
CCCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCCHHHCCHHHCCCCEEEEECEEE
TPDHNVPTTKAERQGSLLSIADTVSRLQVQTLDENCDDFGIFEFKMNDVRQGIVHVIGPE
CCCCCCCCCCHHHCCCEEHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHCCCEEEECCC
QGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQCLVTQKMKNMQVRVEGTL
CCCCCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECC
PWGVTAKDIVLALIGKIGTAGGNGYAVEFSGSTIRALSMEGRMTICNMAIEAGARVGMVA
CCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCCEEEEHHHHHCCCCEEEEE
VDEKTIQYVHGRPFAPKGSDWDAAVAFWRGLVSDPDAHFDRVVELSAEEIKPQVTWGTSP
ECHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCCCH
EMVSAVDQSVPDPERETDPVKKESLIRALKYMGLQPNDPITSIKLDRVFIGSCTNSRIED
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCHHHHH
LRAAAEVVKGRKVASTVKQAMVVPGSGLVKAQAEVEGLDKIFIEAGFEWREPGCSMCLAM
HHHHHHHHHCHHHHHHHHHHEECCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
NPDKLGSGEHCASTSNRNFEGRQGIGGRTHLVSPAMAAAAAVAGHFVDVREMMR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA