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The map label for this gene is leuB [H]

Identifier: 182682008

GI number: 182682008

Start: 1636471

End: 1637544

Strand: Reverse

Name: leuB [H]

Synonym: XfasM23_1483

Alternate gene names: 182682008

Gene position: 1637544-1636471 (Counterclockwise)

Preceding gene: 182682009

Following gene: 182682007

Centisome position: 64.58

GC content: 49.35

Gene sequence:

>1074_bases
ATGAGTAAGAAAATTTTGATTCTCCCCGGCGATGGCATTGGACATGAAATCGTTGCTGAAGCCGTTAAAGTGTTAGAGCA
TGTTAATGTGCGTCATACGCTGAATATGAGGTTGAGTTTCGACGCACTTGGTGGAGCTGCTTATGAAAAATATGGAAGTC
CACTGGCTGACGAAACCTTGGCGCGTGCACGTGAGGCGGATGCAGTGTTGCTTGGTGCGGTCGGTGGACCACAGTGGGAT
ACGATTGATCCGGCACTTCGTCCAGAGCGGGGGTTGCTGAAGATTCGTGCTCAATTAGGGCTGTTCGCCAATCTGCGTCC
TGCGTTGTTGTACCCGCAATTGGCAGATGCATCCACCCTTAAACCTGAAGTTGTTGCCGGACTCGATATTCTTATCGTGC
GTGAACTGACAGGTGGCCTTTATTTTGGACAACCACGCGGCAGCCGCATATCAAAAAATGGCGAGCGTGAAGCGTTTGAT
ACGTTGCCGTATTGCGAGAGTGAAATCCGTCGTATTCTCAAGGTTGGTTTTGAAATGGCGCGTCTGCGTGGTAAGAAACT
CTGCTCTGTGGACAAAGCAAATGTGCTTGCTTCCAGTCAGTTGTGGCGCACAGTGGCGGGAGAAATGGCTAAAGATTATC
CAGATGTGACCCTCTCGCACATGTATGTGGATAACGCAGCAATGCAATTAGTGCGTTATCCAAAACAGTTTGATGTGATT
GTCACTGAAAATATGTTTGGTGACATTCTTTCGGATCAGGCGTCAATGTTGACCGGTTCGATTGGTATGTTGCCGTCTGC
GTCGTTGGACGCCAATAACAAAGGGATGTACGAGCCGTGCCATGGTTCGGCGCCCGATATTGCAGGACAAGGGGTCGCCA
ATCCATTAGCGACTATTTTATCAGTGGCGATGTTATTGCGTTATGGCATGGGATATATCAAAACAGCGGATGCGATTGAA
TATGCGGTTGGTGTCGTCCTTGATAGTGGTTTGCGTACAGCCGATATTTGGTCCGAAGGGATGACCAAGGTAGGAACTGT
AGCGATGGGAGATGCAGTGGTCGCAGCGCTCTAA

Upstream 100 bases:

>100_bases
CTTAAGATGTATCGTGCGCCTTTTTACCCTTTCTCAACATCTATGAGCAACATGCCTAGCAGAGCTGCTGCCTTATTTAA
TACATAGGACATCATCCAGA

Downstream 100 bases:

>100_bases
GTCGTATTAATCAGTGTGGTTTGATAAACGCTAGTTTTCTTCTATGGGTTACATGCAATATTGATGTAGCCCAATGCTGA
TTTCTTAAGTTACGGCATCA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSKKILILPGDGIGHEIVAEAVKVLEHVNVRHTLNMRLSFDALGGAAYEKYGSPLADETLARAREADAVLLGAVGGPQWD
TIDPALRPERGLLKIRAQLGLFANLRPALLYPQLADASTLKPEVVAGLDILIVRELTGGLYFGQPRGSRISKNGEREAFD
TLPYCESEIRRILKVGFEMARLRGKKLCSVDKANVLASSQLWRTVAGEMAKDYPDVTLSHMYVDNAAMQLVRYPKQFDVI
VTENMFGDILSDQASMLTGSIGMLPSASLDANNKGMYEPCHGSAPDIAGQGVANPLATILSVAMLLRYGMGYIKTADAIE
YAVGVVLDSGLRTADIWSEGMTKVGTVAMGDAVVAAL

Sequences:

>Translated_357_residues
MSKKILILPGDGIGHEIVAEAVKVLEHVNVRHTLNMRLSFDALGGAAYEKYGSPLADETLARAREADAVLLGAVGGPQWD
TIDPALRPERGLLKIRAQLGLFANLRPALLYPQLADASTLKPEVVAGLDILIVRELTGGLYFGQPRGSRISKNGEREAFD
TLPYCESEIRRILKVGFEMARLRGKKLCSVDKANVLASSQLWRTVAGEMAKDYPDVTLSHMYVDNAAMQLVRYPKQFDVI
VTENMFGDILSDQASMLTGSIGMLPSASLDANNKGMYEPCHGSAPDIAGQGVANPLATILSVAMLLRYGMGYIKTADAIE
YAVGVVLDSGLRTADIWSEGMTKVGTVAMGDAVVAAL
>Mature_356_residues
SKKILILPGDGIGHEIVAEAVKVLEHVNVRHTLNMRLSFDALGGAAYEKYGSPLADETLARAREADAVLLGAVGGPQWDT
IDPALRPERGLLKIRAQLGLFANLRPALLYPQLADASTLKPEVVAGLDILIVRELTGGLYFGQPRGSRISKNGEREAFDT
LPYCESEIRRILKVGFEMARLRGKKLCSVDKANVLASSQLWRTVAGEMAKDYPDVTLSHMYVDNAAMQLVRYPKQFDVIV
TENMFGDILSDQASMLTGSIGMLPSASLDANNKGMYEPCHGSAPDIAGQGVANPLATILSVAMLLRYGMGYIKTADAIEY
AVGVVLDSGLRTADIWSEGMTKVGTVAMGDAVVAAL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=330, Percent_Identity=30.9090909090909, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI4758582, Length=346, Percent_Identity=28.9017341040462, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI28178821, Length=307, Percent_Identity=27.0358306188925, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI28178816, Length=310, Percent_Identity=27.0967741935484, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI28178838, Length=332, Percent_Identity=28.6144578313253, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI28178819, Length=141, Percent_Identity=34.0425531914894, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI87081683, Length=357, Percent_Identity=51.8207282913165, Blast_Score=353, Evalue=7e-99,
Organism=Escherichia coli, GI1788101, Length=345, Percent_Identity=34.7826086956522, Blast_Score=181, Evalue=8e-47,
Organism=Escherichia coli, GI1787381, Length=400, Percent_Identity=24.5, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=344, Percent_Identity=31.3953488372093, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25144293, Length=339, Percent_Identity=29.4985250737463, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17505779, Length=344, Percent_Identity=29.6511627906977, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17550882, Length=364, Percent_Identity=28.8461538461538, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319830, Length=362, Percent_Identity=46.9613259668508, Blast_Score=295, Evalue=8e-81,
Organism=Saccharomyces cerevisiae, GI6322097, Length=351, Percent_Identity=35.6125356125356, Blast_Score=173, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6324709, Length=264, Percent_Identity=35.6060606060606, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324291, Length=339, Percent_Identity=31.2684365781711, Blast_Score=120, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24643268, Length=343, Percent_Identity=31.4868804664723, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24643270, Length=343, Percent_Identity=31.4868804664723, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24661184, Length=345, Percent_Identity=28.4057971014493, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161078637, Length=246, Percent_Identity=29.6747967479675, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI161078633, Length=246, Percent_Identity=29.6747967479675, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24650122, Length=246, Percent_Identity=29.6747967479675, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI161078639, Length=246, Percent_Identity=29.6747967479675, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI161078635, Length=246, Percent_Identity=29.6747967479675, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI281362242, Length=352, Percent_Identity=26.9886363636364, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648872, Length=352, Percent_Identity=26.9886363636364, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI20130355, Length=343, Percent_Identity=23.3236151603499, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38380; Mature: 38249

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00470 IDH_IMDH ; PS00307 LECTIN_LEGUME_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKILILPGDGIGHEIVAEAVKVLEHVNVRHTLNMRLSFDALGGAAYEKYGSPLADETL
CCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEEHHHCCHHHHHHCCCHHHHHH
ARAREADAVLLGAVGGPQWDTIDPALRPERGLLKIRAQLGLFANLRPALLYPQLADASTL
HHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCC
KPEVVAGLDILIVRELTGGLYFGQPRGSRISKNGEREAFDTLPYCESEIRRILKVGFEMA
CHHHHHCCHHEEEEHHHCCEEECCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHH
RLRGKKLCSVDKANVLASSQLWRTVAGEMAKDYPDVTLSHMYVDNAAMQLVRYPKQFDVI
HHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHEEHHHHHHHHHHCCCHHEEE
VTENMFGDILSDQASMLTGSIGMLPSASLDANNKGMYEPCHGSAPDIAGQGVANPLATIL
EECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
SVAMLLRYGMGYIKTADAIEYAVGVVLDSGLRTADIWSEGMTKVGTVAMGDAVVAAL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHCCEEECCCHHHCC
>Mature Secondary Structure 
SKKILILPGDGIGHEIVAEAVKVLEHVNVRHTLNMRLSFDALGGAAYEKYGSPLADETL
CCEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEEHHHCCHHHHHHCCCHHHHHH
ARAREADAVLLGAVGGPQWDTIDPALRPERGLLKIRAQLGLFANLRPALLYPQLADASTL
HHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCC
KPEVVAGLDILIVRELTGGLYFGQPRGSRISKNGEREAFDTLPYCESEIRRILKVGFEMA
CHHHHHCCHHEEEEHHHCCEEECCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHH
RLRGKKLCSVDKANVLASSQLWRTVAGEMAKDYPDVTLSHMYVDNAAMQLVRYPKQFDVI
HHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHEEHHHHHHHHHHCCCHHEEE
VTENMFGDILSDQASMLTGSIGMLPSASLDANNKGMYEPCHGSAPDIAGQGVANPLATIL
EECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
SVAMLLRYGMGYIKTADAIEYAVGVVLDSGLRTADIWSEGMTKVGTVAMGDAVVAAL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHCCEEECCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]