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The map label for this gene is leuD

Identifier: 182682009

GI number: 182682009

Start: 1638129

End: 1638776

Strand: Reverse

Name: leuD

Synonym: XfasM23_1484

Alternate gene names: 182682009

Gene position: 1638776-1638129 (Counterclockwise)

Preceding gene: 182682010

Following gene: 182682008

Centisome position: 64.63

GC content: 49.23

Gene sequence:

>648_bases
ATGAAACCCTTTACCCAACACACCGGCTTGGTCTGTCCACTTGATCGCGTGAATGTCGATACCGATCAGATTATTCCCAA
GCAATTTTTAAAGTCGATCAAGCGGACAGGTTTCGGCCCTAATCTGTTCGACGAATGGCGCTATTTGGATGCAGGTCAAC
CTGGGCAAGACAATAGCAAGCGTCCCATTAATTCAGATTTTGTGCTCAATTTTCCCCGTTATCGTGGGGCGAGTGTGTTG
CTGGCGCGCGACAATTTTGGCTGTGGTTCCTCCCGTGAACATGCTGCCTGGGCGCTTGATGAATATGGCTTCCGCACAGT
CATCGCGCCGAGCTTTGCTGACATTTTTTTCAACAACAGCTTTAAAAATGGTTTATTGCCTCTTGTGTTGAATAAGGTTG
AAGTGGATGCACTGTTTGCACAGTGTCAAGTGACTGAGGGATATACGCTAACTGTGGATTTGGCGGCACAGCAAGTGATT
ACCCTGGACGGTACTACTTACGCCTTCCAGATCGATACGTTCCGTAAGCATTGTCTACTTAACGGCCTGGATGATATCGG
TCTCACCTTACAGCATGCTGAGGCCATCCGCGCTTTTGAGGCCACGCATCGTATACGTCAACCGTGGTTGTTTGCGCCGT
TGCGCTGA

Upstream 100 bases:

>100_bases
CTCTAAACGGTTATTTTTTTCGATTCCAGCAGCGTAATAAAACGGTTCTGCGATCTATGATCGCAGCGTACATAGAATAT
TTAAGCACTGGTGAAGCTTG

Downstream 100 bases:

>100_bases
ATCCTTGTTGATCAATATGACTCTACAGAAGGCAGTAATTTATTTCTGTGGAGCTTCGTGCGTGTTGCTCAGCGTATCTG
CGAGATTACGGTGAGTGTCC

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MKPFTQHTGLVCPLDRVNVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDAGQPGQDNSKRPINSDFVLNFPRYRGASVL
LARDNFGCGSSREHAAWALDEYGFRTVIAPSFADIFFNNSFKNGLLPLVLNKVEVDALFAQCQVTEGYTLTVDLAAQQVI
TLDGTTYAFQIDTFRKHCLLNGLDDIGLTLQHAEAIRAFEATHRIRQPWLFAPLR

Sequences:

>Translated_215_residues
MKPFTQHTGLVCPLDRVNVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDAGQPGQDNSKRPINSDFVLNFPRYRGASVL
LARDNFGCGSSREHAAWALDEYGFRTVIAPSFADIFFNNSFKNGLLPLVLNKVEVDALFAQCQVTEGYTLTVDLAAQQVI
TLDGTTYAFQIDTFRKHCLLNGLDDIGLTLQHAEAIRAFEATHRIRQPWLFAPLR
>Mature_215_residues
MKPFTQHTGLVCPLDRVNVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDAGQPGQDNSKRPINSDFVLNFPRYRGASVL
LARDNFGCGSSREHAAWALDEYGFRTVIAPSFADIFFNNSFKNGLLPLVLNKVEVDALFAQCQVTEGYTLTVDLAAQQVI
TLDGTTYAFQIDTFRKHCLLNGLDDIGLTLQHAEAIRAFEATHRIRQPWLFAPLR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=207, Percent_Identity=59.9033816425121, Blast_Score=249, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6321429, Length=214, Percent_Identity=50.4672897196262, Blast_Score=208, Evalue=5e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_XYLF2 (B2I6I5)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830169.1
- ProteinModelPortal:   B2I6I5
- GeneID:   6202493
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1484
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 24283; Mature: 24283

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPFTQHTGLVCPLDRVNVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDAGQPGQDNSK
CCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCEECCCCCCCCCCCC
RPINSDFVLNFPRYRGASVLLARDNFGCGSSREHAAWALDEYGFRTVIAPSFADIFFNNS
CCCCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCEEEHHHCCCEEEECCCHHHHHCCCC
FKNGLLPLVLNKVEVDALFAQCQVTEGYTLTVDLAAQQVITLDGTTYAFQIDTFRKHCLL
CCCCCHHHHHHHHHHHHHEEEEEECCCEEEEEEECCEEEEEECCCEEEEEEHHHHHHHHH
NGLDDIGLTLQHAEAIRAFEATHRIRQPWLFAPLR
CCCCHHCEEHHHHHHHHHHHHHHHHCCCEEECCCC
>Mature Secondary Structure
MKPFTQHTGLVCPLDRVNVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDAGQPGQDNSK
CCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCEECCCCCCCCCCCC
RPINSDFVLNFPRYRGASVLLARDNFGCGSSREHAAWALDEYGFRTVIAPSFADIFFNNS
CCCCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCEEEHHHCCCEEEECCCHHHHHCCCC
FKNGLLPLVLNKVEVDALFAQCQVTEGYTLTVDLAAQQVITLDGTTYAFQIDTFRKHCLL
CCCCCHHHHHHHHHHHHHEEEEEECCCEEEEEEECCEEEEEECCCEEEEEEHHHHHHHHH
NGLDDIGLTLQHAEAIRAFEATHRIRQPWLFAPLR
CCCCHHCEEHHHHHHHHHHHHHHHHCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA