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The map label for this gene is grpE

Identifier: 182681981

GI number: 182681981

Start: 1608980

End: 1609498

Strand: Reverse

Name: grpE

Synonym: XfasM23_1456

Alternate gene names: 182681981

Gene position: 1609498-1608980 (Counterclockwise)

Preceding gene: 182681982

Following gene: 182681980

Centisome position: 63.47

GC content: 51.64

Gene sequence:

>519_bases
ATGAACCAAGACCACCCCGAATGTGATTCTGAAGAACTGACGCAGAATTCACCGGAAACAGATCCGCTCAAGGTTGAGGT
TGAGACGTTGCGTGGTGAGATTGCGTCGATTAAGGCGGATGTATTGCGTGAGCGTGCTGAGCTGGAGAATCAGCGCAAGC
GCCTCATTCGAGATGTTGAACAAGCGCGAAAATTCGCTAATGAAAAGCTGCTTGGCGAGTTACTGCCGGTGTTTGACAGT
CTGGATGCGGGATTGACTGCTTCCGGTAGTGAGCCAAGCCCGTTGCGCGATGGTCTGGAGCTGACTTACAAGCAATTGTT
GAAGGTTGCTATTGACAACGGTTTGATGCTTCTAGATCCAGTGGGTCAGCTATTTAACCCCGAGCACCATCAAGCGATCA
GCCAGACGGAGGTTACTGACGTCGAACCAGGGCATGTGATTCAGGTGTTTCAGAAGGGATATCTGCTCAACGAACGTTTG
CTGCGCCCTGCTCTGGTGGTGGTTGCCAAGCAGGATTGA

Upstream 100 bases:

>100_bases
CATCGTTTGATGAATTATGCATGGATGGCTTGAAAGCTGGCGGCTTGCCCACATACGGGTTGGGGTTGAGGCGGGCGTTC
CGCCGACTCAGGATTTTTAT

Downstream 100 bases:

>100_bases
GTTGTTTTGAGTCTTATTGTTCATGCAGTACAACGTCTTGACAGCCGCTTGAGCAGGCGTCGGTCATCCCCACATTCGAA
ACAGATCCCGGCACCGCTGG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MNQDHPECDSEELTQNSPETDPLKVEVETLRGEIASIKADVLRERAELENQRKRLIRDVEQARKFANEKLLGELLPVFDS
LDAGLTASGSEPSPLRDGLELTYKQLLKVAIDNGLMLLDPVGQLFNPEHHQAISQTEVTDVEPGHVIQVFQKGYLLNERL
LRPALVVVAKQD

Sequences:

>Translated_172_residues
MNQDHPECDSEELTQNSPETDPLKVEVETLRGEIASIKADVLRERAELENQRKRLIRDVEQARKFANEKLLGELLPVFDS
LDAGLTASGSEPSPLRDGLELTYKQLLKVAIDNGLMLLDPVGQLFNPEHHQAISQTEVTDVEPGHVIQVFQKGYLLNERL
LRPALVVVAKQD
>Mature_172_residues
MNQDHPECDSEELTQNSPETDPLKVEVETLRGEIASIKADVLRERAELENQRKRLIRDVEQARKFANEKLLGELLPVFDS
LDAGLTASGSEPSPLRDGLELTYKQLLKVAIDNGLMLLDPVGQLFNPEHHQAISQTEVTDVEPGHVIQVFQKGYLLNERL
LRPALVVVAKQD

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=137, Percent_Identity=37.2262773722628, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI1788967, Length=177, Percent_Identity=40.1129943502825, Blast_Score=114, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17552458, Length=161, Percent_Identity=32.2981366459627, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324806, Length=169, Percent_Identity=30.1775147928994, Blast_Score=79, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24653432, Length=171, Percent_Identity=35.672514619883, Blast_Score=94, Evalue=6e-20,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_XYLF2 (B2I6F7)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830141.1
- ProteinModelPortal:   B2I6F7
- SMR:   B2I6F7
- GeneID:   6202416
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1456
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- ProtClustDB:   PRK14147
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 19285; Mature: 19285

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQDHPECDSEELTQNSPETDPLKVEVETLRGEIASIKADVLRERAELENQRKRLIRDVE
CCCCCCCCCHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QARKFANEKLLGELLPVFDSLDAGLTASGSEPSPLRDGLELTYKQLLKVAIDNGLMLLDP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECC
VGQLFNPEHHQAISQTEVTDVEPGHVIQVFQKGYLLNERLLRPALVVVAKQD
HHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHEEECCC
>Mature Secondary Structure
MNQDHPECDSEELTQNSPETDPLKVEVETLRGEIASIKADVLRERAELENQRKRLIRDVE
CCCCCCCCCHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QARKFANEKLLGELLPVFDSLDAGLTASGSEPSPLRDGLELTYKQLLKVAIDNGLMLLDP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECC
VGQLFNPEHHQAISQTEVTDVEPGHVIQVFQKGYLLNERLLRPALVVVAKQD
HHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA