Click here to switch to the map view.

The map label for this gene is dhfrIII [H]

Identifier: 182681973

GI number: 182681973

Start: 1600233

End: 1600799

Strand: Reverse

Name: dhfrIII [H]

Synonym: XfasM23_1448

Alternate gene names: 182681973

Gene position: 1600799-1600233 (Counterclockwise)

Preceding gene: 182681974

Following gene: 182681972

Centisome position: 63.13

GC content: 52.91

Gene sequence:

>567_bases
GTGGAGACGCATCTCAAGAAAGCCAGAGAAAATAATGAATATCTACAACGTGTTGTACGTAGTGCCATGATTGTTTCCTT
AATTGCGGCGTTGGATCGTTACCGCGCCATTGGTCGTGATAATGGGTTGCCGTGGCACTTGCCGGACGATCTGAAGCGTT
TCAAAGCATTGACTTTAGGTCGACCGGTGCTGATGGGACGCAAGACTGCGCATTCCCTTGGACGTCCCTTACCAGGGAGA
ACCAATCTGGTATTGACGCGTTCTGGACACGTGCCATTTTCTGAAATGCGGGCGGTGGATTCGCTTGATGCAGCTATCGT
CCAGGCGGCTGCGGAGGGTGCCGACACGTTAAGTGTGATTGGTGGAGGTGAACTGTATGCACTGACTTTGCCGCACGCCA
ATGCGATGTACTTAACTCACGTGGATGTGGCTGTTGCGGGCGCTGACACGTATTTTCCTATGTTCGACGCCGTCGATTGG
GAGATCAAGAACCGTGAACCCCATCAGGGCGATGGGCGTCATGCCTACCCGTTCACCTTCATAGACTATTGCCGCCGATT
GCGTTAG

Upstream 100 bases:

>100_bases
CATTGTGATTGAAGGTTACGATCCATATCCGGCTATCAAGGCACCGGTGGCTGTGTGATCTGCGGCATCAGTTGGCGACT
CAGACCAGTCGGCGTAATGA

Downstream 100 bases:

>100_bases
TGCATTCCCACGATCTTGTGTTTCTGCAACGCATGCAGTGAGCAGTGACGGGATGCGCGGTGGCTATTGTTCATCAACAG
TATTCAATGAAGTGCGATTG

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
METHLKKARENNEYLQRVVRSAMIVSLIAALDRYRAIGRDNGLPWHLPDDLKRFKALTLGRPVLMGRKTAHSLGRPLPGR
TNLVLTRSGHVPFSEMRAVDSLDAAIVQAAAEGADTLSVIGGGELYALTLPHANAMYLTHVDVAVAGADTYFPMFDAVDW
EIKNREPHQGDGRHAYPFTFIDYCRRLR

Sequences:

>Translated_188_residues
METHLKKARENNEYLQRVVRSAMIVSLIAALDRYRAIGRDNGLPWHLPDDLKRFKALTLGRPVLMGRKTAHSLGRPLPGR
TNLVLTRSGHVPFSEMRAVDSLDAAIVQAAAEGADTLSVIGGGELYALTLPHANAMYLTHVDVAVAGADTYFPMFDAVDW
EIKNREPHQGDGRHAYPFTFIDYCRRLR
>Mature_188_residues
METHLKKARENNEYLQRVVRSAMIVSLIAALDRYRAIGRDNGLPWHLPDDLKRFKALTLGRPVLMGRKTAHSLGRPLPGR
TNLVLTRSGHVPFSEMRAVDSLDAAIVQAAAEGADTLSVIGGGELYALTLPHANAMYLTHVDVAVAGADTYFPMFDAVDW
EIKNREPHQGDGRHAYPFTFIDYCRRLR

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=159, Percent_Identity=45.2830188679245, Blast_Score=130, Evalue=5e-32,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 20993; Mature: 20993

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METHLKKARENNEYLQRVVRSAMIVSLIAALDRYRAIGRDNGLPWHLPDDLKRFKALTLG
CCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCC
RPVLMGRKTAHSLGRPLPGRTNLVLTRSGHVPFSEMRAVDSLDAAIVQAAAEGADTLSVI
CHHHHCCHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
GGGELYALTLPHANAMYLTHVDVAVAGADTYFPMFDAVDWEIKNREPHQGDGRHAYPFTF
CCCEEEEEECCCCCEEEEEEEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCEECCHHH
IDYCRRLR
HHHHHHCC
>Mature Secondary Structure
METHLKKARENNEYLQRVVRSAMIVSLIAALDRYRAIGRDNGLPWHLPDDLKRFKALTLG
CCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCC
RPVLMGRKTAHSLGRPLPGRTNLVLTRSGHVPFSEMRAVDSLDAAIVQAAAEGADTLSVI
CHHHHCCHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
GGGELYALTLPHANAMYLTHVDVAVAGADTYFPMFDAVDWEIKNREPHQGDGRHAYPFTF
CCCEEEEEECCCCCEEEEEEEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCEECCHHH
IDYCRRLR
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]