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The map label for this gene is thyA

Identifier: 182681974

GI number: 182681974

Start: 1600842

End: 1601636

Strand: Reverse

Name: thyA

Synonym: XfasM23_1449

Alternate gene names: 182681974

Gene position: 1601636-1600842 (Counterclockwise)

Preceding gene: 182681975

Following gene: 182681973

Centisome position: 63.16

GC content: 50.31

Gene sequence:

>795_bases
GTGAAGCAGTACCTTGAATTACTGAATGATGTGCTTGTGCATGGCATAAAAAAGCCTGACCGCACTGGCACGGGGACACG
CAGTGTGTTCGGTTGGCAGATGCGATTTGACTTGAGCCAAGGTTTTCCACTCGTTACGACTAAAAAGCTGCATTTGCGTT
CGATCATTCACGAGTTGCTATGGTTTCTCAGGGGGGAAACCAATATTGCTTATCTTAAGAAGCATCAGGTACACATCTGG
GACGAGTGGGCCGATGCCACGGGTGAGTTGGGACCTGTTTACGGCAAGCAATGGCGCCGTTGGGCTGGTGCGGATGGTCA
CGAGATCGACCAGATCCGTTGGTTGGTGGAGGAGATCAAGCGTAATCCAGATTCGCGCCGCTTGGTGATTAGTGCTTGGA
ATGTGGCTGATCTTCCACAGATGGCTTTGGTGCCGTGCCATGCACTGTTTCAATTCTATGTTGCCAATGGCAAATTGAGC
TGTCAGCTCTATCAACGTAGCGCTGATATTTTTCTTGGCGTCCCTTTCAATATCGCTAGTTATGCACTGCTCACCCATAT
GTTGGCTCAGGTGACTGGGCTCGTGGTCGGTGATTTTGTGCATACGTTGGGTGATGCACATTTGTATGCTAATCATGTCG
AGCAAGCATCCGTGCAACTGGGGCGTGTGCCGCGACCGCAACCGGTATTAAGGCTTAATCAGGCAGTGACTGACTTGTTC
GACTTTACCTACGATGACATTGTGATTGAAGGTTACGATCCATATCCGGCTATCAAGGCACCGGTGGCTGTGTGA

Upstream 100 bases:

>100_bases
AGATCTTAAGCTTGCCGTTGATCGTGATTGGATTGTTCCTGTTCTGGTTGTCGTGCCGCTCACCAGTATTACAGCCAGTG
CCTGCCCCTGAGGTCGCTAA

Downstream 100 bases:

>100_bases
TCTGCGGCATCAGTTGGCGACTCAGACCAGTCGGCGTAATGAGTGGAGACGCATCTCAAGAAAGCCAGAGAAAATAATGA
ATATCTACAACGTGTTGTAC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKQYLELLNDVLVHGIKKPDRTGTGTRSVFGWQMRFDLSQGFPLVTTKKLHLRSIIHELLWFLRGETNIAYLKKHQVHIW
DEWADATGELGPVYGKQWRRWAGADGHEIDQIRWLVEEIKRNPDSRRLVISAWNVADLPQMALVPCHALFQFYVANGKLS
CQLYQRSADIFLGVPFNIASYALLTHMLAQVTGLVVGDFVHTLGDAHLYANHVEQASVQLGRVPRPQPVLRLNQAVTDLF
DFTYDDIVIEGYDPYPAIKAPVAV

Sequences:

>Translated_264_residues
MKQYLELLNDVLVHGIKKPDRTGTGTRSVFGWQMRFDLSQGFPLVTTKKLHLRSIIHELLWFLRGETNIAYLKKHQVHIW
DEWADATGELGPVYGKQWRRWAGADGHEIDQIRWLVEEIKRNPDSRRLVISAWNVADLPQMALVPCHALFQFYVANGKLS
CQLYQRSADIFLGVPFNIASYALLTHMLAQVTGLVVGDFVHTLGDAHLYANHVEQASVQLGRVPRPQPVLRLNQAVTDLF
DFTYDDIVIEGYDPYPAIKAPVAV
>Mature_264_residues
MKQYLELLNDVLVHGIKKPDRTGTGTRSVFGWQMRFDLSQGFPLVTTKKLHLRSIIHELLWFLRGETNIAYLKKHQVHIW
DEWADATGELGPVYGKQWRRWAGADGHEIDQIRWLVEEIKRNPDSRRLVISAWNVADLPQMALVPCHALFQFYVANGKLS
CQLYQRSADIFLGVPFNIASYALLTHMLAQVTGLVVGDFVHTLGDAHLYANHVEQASVQLGRVPRPQPVLRLNQAVTDLF
DFTYDDIVIEGYDPYPAIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=282, Percent_Identity=51.4184397163121, Blast_Score=291, Evalue=5e-79,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=65.530303030303, Blast_Score=374, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=45.9363957597173, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6324648, Length=230, Percent_Identity=44.3478260869565, Blast_Score=191, Evalue=1e-49,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=51.5901060070671, Blast_Score=290, Evalue=7e-79,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 30084; Mature: 30084

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLELLNDVLVHGIKKPDRTGTGTRSVFGWQMRFDLSQGFPLVTTKKLHLRSIIHELL
CHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEHHHHHHHHHHHHHH
WFLRGETNIAYLKKHQVHIWDEWADATGELGPVYGKQWRRWAGADGHEIDQIRWLVEEIK
HHHCCCCCEEEEEECEEEEEECHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
RNPDSRRLVISAWNVADLPQMALVPCHALFQFYVANGKLSCQLYQRSADIFLGVPFNIAS
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEECCHHHHH
YALLTHMLAQVTGLVVGDFVHTLGDAHLYANHVEQASVQLGRVPRPQPVLRLNQAVTDLF
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
DFTYDDIVIEGYDPYPAIKAPVAV
HCCCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKQYLELLNDVLVHGIKKPDRTGTGTRSVFGWQMRFDLSQGFPLVTTKKLHLRSIIHELL
CHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEHHHHHHHHHHHHHH
WFLRGETNIAYLKKHQVHIWDEWADATGELGPVYGKQWRRWAGADGHEIDQIRWLVEEIK
HHHCCCCCEEEEEECEEEEEECHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
RNPDSRRLVISAWNVADLPQMALVPCHALFQFYVANGKLSCQLYQRSADIFLGVPFNIAS
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEECCHHHHH
YALLTHMLAQVTGLVVGDFVHTLGDAHLYANHVEQASVQLGRVPRPQPVLRLNQAVTDLF
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
DFTYDDIVIEGYDPYPAIKAPVAV
HCCCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]