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The map label for this gene is pncA [H]

Identifier: 182681926

GI number: 182681926

Start: 1551901

End: 1552527

Strand: Direct

Name: pncA [H]

Synonym: XfasM23_1394

Alternate gene names: 182681926

Gene position: 1551901-1552527 (Clockwise)

Preceding gene: 182681925

Following gene: 182681935

Centisome position: 61.2

GC content: 60.45

Gene sequence:

>627_bases
ATGCACCCTGAAAACGTCGCATTGATCGTCGTCGACCTACAGCCTGATTTCATGCCAGGCGGCGCACTGCCATGCGAGGA
AGGTGCATCCATTGTCCCCGGAATCGCCGCACTCTTGGATGCGCGCCAGTACTCTACGATCGTTGCAACGCAAGATTGGC
ACCCGGCTGACCACGTTTCGTTTGCCTCGCAACATCCCGCACAGCGACCGTTTGAGCAAATCACCCTGTATGGCCAACCC
CAGACCCTATGGCCCGATCACTGCATCCAGGGCAGCGCCGCAGCGGGGCTTGATAGCCGTGTTGATTGGCGGGCGGCTGA
TCTGATTTTGCGCAAAGGCACCCGCCGCGACACCGACTCTTATAGCGCGTTCCGCGAAAACCACGGTCCCAACGGCGACC
GCCCGGCCACTGGTCTGGCTGGCTGGTTGCGTGAGCGCGGTATTGAAGAAGTCCACGTCTGCGGGCTGGCCCGTGACTAC
TGTGTCCTGTGGACTGCGCAAGATGCCCTCTCGGCAGGATTCAAAGTCCGTTTTCTGTGGCCGCTGACACGTCCCGTCAC
CCCTGAGGGTGACGCCCCAACCCGTAAAGCGCTTGAAACCCTAGGGATCATCATTGATCACGGCTGA

Upstream 100 bases:

>100_bases
CGACACCCCTGAATCTGCAAACACAAACAGTACATCAGGTGTCACCCCAACAGAGACAACGGATTAAACTGCCTCACCTG
TTATCTCAATGGAGCACTTC

Downstream 100 bases:

>100_bases
AGATCAATCCTCGCAACCCACATTGCACCACCGCCAAGGATGTCACAGAATCGACTCCTCACTACTGAAAACAATCAGCA
GCGCAACCGTTGTGCTGCCC

Product: nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MHPENVALIVVDLQPDFMPGGALPCEEGASIVPGIAALLDARQYSTIVATQDWHPADHVSFASQHPAQRPFEQITLYGQP
QTLWPDHCIQGSAAAGLDSRVDWRAADLILRKGTRRDTDSYSAFRENHGPNGDRPATGLAGWLRERGIEEVHVCGLARDY
CVLWTAQDALSAGFKVRFLWPLTRPVTPEGDAPTRKALETLGIIIDHG

Sequences:

>Translated_208_residues
MHPENVALIVVDLQPDFMPGGALPCEEGASIVPGIAALLDARQYSTIVATQDWHPADHVSFASQHPAQRPFEQITLYGQP
QTLWPDHCIQGSAAAGLDSRVDWRAADLILRKGTRRDTDSYSAFRENHGPNGDRPATGLAGWLRERGIEEVHVCGLARDY
CVLWTAQDALSAGFKVRFLWPLTRPVTPEGDAPTRKALETLGIIIDHG
>Mature_208_residues
MHPENVALIVVDLQPDFMPGGALPCEEGASIVPGIAALLDARQYSTIVATQDWHPADHVSFASQHPAQRPFEQITLYGQP
QTLWPDHCIQGSAAAGLDSRVDWRAADLILRKGTRRDTDSYSAFRENHGPNGDRPATGLAGWLRERGIEEVHVCGLARDY
CVLWTAQDALSAGFKVRFLWPLTRPVTPEGDAPTRKALETLGIIIDHG

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=183, Percent_Identity=45.9016393442623, Blast_Score=141, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI193206735, Length=220, Percent_Identity=33.1818181818182, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17543130, Length=220, Percent_Identity=33.1818181818182, Blast_Score=98, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71999339, Length=191, Percent_Identity=34.5549738219895, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6321401, Length=213, Percent_Identity=32.8638497652582, Blast_Score=103, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648247, Length=222, Percent_Identity=31.5315315315315, Blast_Score=105, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22795; Mature: 22795

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPENVALIVVDLQPDFMPGGALPCEEGASIVPGIAALLDARQYSTIVATQDWHPADHVS
CCCCCEEEEEEECCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHEEEEEECCCCCCHHCC
FASQHPAQRPFEQITLYGQPQTLWPDHCIQGSAAAGLDSRVDWRAADLILRKGTRRDTDS
CCCCCCCCCCHHHEEEECCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHH
YSAFRENHGPNGDRPATGLAGWLRERGIEEVHVCGLARDYCVLWTAQDALSAGFKVRFLW
HHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHCCCCCEEEEEECHHHHHCCCEEEEEE
PLTRPVTPEGDAPTRKALETLGIIIDHG
ECCCCCCCCCCCHHHHHHHHHCEEEECC
>Mature Secondary Structure
MHPENVALIVVDLQPDFMPGGALPCEEGASIVPGIAALLDARQYSTIVATQDWHPADHVS
CCCCCEEEEEEECCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHEEEEEECCCCCCHHCC
FASQHPAQRPFEQITLYGQPQTLWPDHCIQGSAAAGLDSRVDWRAADLILRKGTRRDTDS
CCCCCCCCCCHHHEEEECCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHH
YSAFRENHGPNGDRPATGLAGWLRERGIEEVHVCGLARDYCVLWTAQDALSAGFKVRFLW
HHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHCCCCCEEEEEECHHHHHCCCEEEEEE
PLTRPVTPEGDAPTRKALETLGIIIDHG
ECCCCCCCCCCCHHHHHHHHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]