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The map label for this gene is mltB [H]

Identifier: 182681850

GI number: 182681850

Start: 1448197

End: 1449309

Strand: Direct

Name: mltB [H]

Synonym: XfasM23_1315

Alternate gene names: 182681850

Gene position: 1448197-1449309 (Clockwise)

Preceding gene: 182681848

Following gene: 182681851

Centisome position: 57.11

GC content: 51.12

Gene sequence:

>1113_bases
ATGATTAAGTGCATAGCAACCTCGATACTGACTCTCGGCCTCGTTGCCTGTGCATCGCAACCCTCATCCAAGCGGCTAGT
CAGTACAACGACAAAAGAACCAACGAAAAAGATAGATCCCTCAAAAGTGGAAGAAACACCACTAAATCTGTCGCCTGTCC
CATTAGAAATCGCGCGTAACAATTTCATACGTGATACTGCAACCAAGTACAACTTGGACCCGATATGGATCGAAAAAACC
CTAGCACAAGCTCAATTTAAAGACGCAATAGTAGCGGCCATGTCGCGCCCAGCCGAGCAAGTCAAACCGTGGAACGAATA
CCGATCAATGTTCATCGCTCAGGCAAGAATTGACGGTGGACGTCAGTTCTTAGCCTCTCACAAGATACAACTCAAAAAAG
TCGAAGCTGCTACAGGCGTACCAGCAGAACTCATCGTCGCAATTATTGGTGTCGAAAGCAGCTATGGTACTAGCCCCGGG
AAATACCGCGTACTGGACGCGCTATACACACTCGCCTTTAAATACCCGCGCAGCGGCGACCCAAACAAACTCAAACGCGA
AGTACAACGTGAATTATTTTTTCGCGATGAACTCGCCCAACTATTCGCACTCAGTAAAGAAGAAAACCTGGATATCACCA
CATTGACTGGCAGCTACGCCGGAGCAATGGGCATGGGACAGTTCATGCCCTCCAGCTATCGTCAATATGCAGTAGACGGC
GATGGGGACGGCAAACGCAACCTATTCACCAACGAAGATGACGTATTCGCTTCGATCGCCAACTACTTCCTCCGAAAAGG
AGGCTGGGTACGTGGCGCTCTGGTCGCAGTACCAGCCACCCTCGCTTCGGGTTACCAAGAATTCGACCCACCCGACTGGA
TCCCGCGTTACACCCTGACCGAATTAGCCGCACGTGGCTACCATACCTATGTGCCCGTCACAGCCGGTATCACCGCCACC
CCGATCAGTCTGGACAACACCACCAGTAAAGAATACTGGCTTTGCTTCCAGAACTACTACGCGATTACCCGCTACAACCC
CTCCAAAATGTACGCCATGGCCGTGTTCCAACTGTCCGAATCCATTGCCGGCCGCACACTCCCCAAAACATGA

Upstream 100 bases:

>100_bases
TATCGGAAACCAACAATAGAACTCATCCAACTTGACAAAAAACGAAACACACACCTTTATTAACTTATTTTTTATTTATC
TTCCCTTATTGTTAATAAGT

Downstream 100 bases:

>100_bases
ACACGCGCTGGTACTTCATAGCACTTGTATTTGACCTGATGGCGTGTAGCACCACACCACACAAAAGTGGCACATACCCA
TCCCCAGCCACCTCCAAAAT

Product: lytic murein transglycosylase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MIKCIATSILTLGLVACASQPSSKRLVSTTTKEPTKKIDPSKVEETPLNLSPVPLEIARNNFIRDTATKYNLDPIWIEKT
LAQAQFKDAIVAAMSRPAEQVKPWNEYRSMFIAQARIDGGRQFLASHKIQLKKVEAATGVPAELIVAIIGVESSYGTSPG
KYRVLDALYTLAFKYPRSGDPNKLKREVQRELFFRDELAQLFALSKEENLDITTLTGSYAGAMGMGQFMPSSYRQYAVDG
DGDGKRNLFTNEDDVFASIANYFLRKGGWVRGALVAVPATLASGYQEFDPPDWIPRYTLTELAARGYHTYVPVTAGITAT
PISLDNTTSKEYWLCFQNYYAITRYNPSKMYAMAVFQLSESIAGRTLPKT

Sequences:

>Translated_370_residues
MIKCIATSILTLGLVACASQPSSKRLVSTTTKEPTKKIDPSKVEETPLNLSPVPLEIARNNFIRDTATKYNLDPIWIEKT
LAQAQFKDAIVAAMSRPAEQVKPWNEYRSMFIAQARIDGGRQFLASHKIQLKKVEAATGVPAELIVAIIGVESSYGTSPG
KYRVLDALYTLAFKYPRSGDPNKLKREVQRELFFRDELAQLFALSKEENLDITTLTGSYAGAMGMGQFMPSSYRQYAVDG
DGDGKRNLFTNEDDVFASIANYFLRKGGWVRGALVAVPATLASGYQEFDPPDWIPRYTLTELAARGYHTYVPVTAGITAT
PISLDNTTSKEYWLCFQNYYAITRYNPSKMYAMAVFQLSESIAGRTLPKT
>Mature_370_residues
MIKCIATSILTLGLVACASQPSSKRLVSTTTKEPTKKIDPSKVEETPLNLSPVPLEIARNNFIRDTATKYNLDPIWIEKT
LAQAQFKDAIVAAMSRPAEQVKPWNEYRSMFIAQARIDGGRQFLASHKIQLKKVEAATGVPAELIVAIIGVESSYGTSPG
KYRVLDALYTLAFKYPRSGDPNKLKREVQRELFFRDELAQLFALSKEENLDITTLTGSYAGAMGMGQFMPSSYRQYAVDG
DGDGKRNLFTNEDDVFASIANYFLRKGGWVRGALVAVPATLASGYQEFDPPDWIPRYTLTELAARGYHTYVPVTAGITAT
PISLDNTTSKEYWLCFQNYYAITRYNPSKMYAMAVFQLSESIAGRTLPKT

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=311, Percent_Identity=39.2282958199357, Blast_Score=201, Evalue=9e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 41144; Mature: 41144

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKCIATSILTLGLVACASQPSSKRLVSTTTKEPTKKIDPSKVEETPLNLSPVPLEIARN
CCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCHHHCCCCCCCCCCHHHHCC
NFIRDTATKYNLDPIWIEKTLAQAQFKDAIVAAMSRPAEQVKPWNEYRSMFIAQARIDGG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHCCH
RQFLASHKIQLKKVEAATGVPAELIVAIIGVESSYGTSPGKYRVLDALYTLAFKYPRSGD
HHHHHHCCEEEEEEHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
PNKLKREVQRELFFRDELAQLFALSKEENLDITTLTGSYAGAMGMGQFMPSSYRQYAVDG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHCCCCCCCCCCCCCCEEEECC
DGDGKRNLFTNEDDVFASIANYFLRKGGWVRGALVAVPATLASGYQEFDPPDWIPRYTLT
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCHHHCCCCCCCCCHHHH
ELAARGYHTYVPVTAGITATPISLDNTTSKEYWLCFQNYYAITRYNPSKMYAMAVFQLSE
HHHHCCCEEEEEEECCCEECCEECCCCCCCCEEEEEECEEEEEEECHHHHHHHHHHHHHH
SIAGRTLPKT
HHCCCCCCCC
>Mature Secondary Structure
MIKCIATSILTLGLVACASQPSSKRLVSTTTKEPTKKIDPSKVEETPLNLSPVPLEIARN
CCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCHHHCCCCCCCCCCHHHHCC
NFIRDTATKYNLDPIWIEKTLAQAQFKDAIVAAMSRPAEQVKPWNEYRSMFIAQARIDGG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHCCH
RQFLASHKIQLKKVEAATGVPAELIVAIIGVESSYGTSPGKYRVLDALYTLAFKYPRSGD
HHHHHHCCEEEEEEHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
PNKLKREVQRELFFRDELAQLFALSKEENLDITTLTGSYAGAMGMGQFMPSSYRQYAVDG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHCCCCCCCCCCCCCCEEEECC
DGDGKRNLFTNEDDVFASIANYFLRKGGWVRGALVAVPATLASGYQEFDPPDWIPRYTLT
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCHHHCCCCCCCCCHHHH
ELAARGYHTYVPVTAGITATPISLDNTTSKEYWLCFQNYYAITRYNPSKMYAMAVFQLSE
HHHHCCCEEEEEEECCCEECCEECCCCCCCCEEEEEECEEEEEEECHHHHHHHHHHHHHH
SIAGRTLPKT
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]