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The map label for this gene is rnt [H]

Identifier: 182681822

GI number: 182681822

Start: 1418571

End: 1419203

Strand: Direct

Name: rnt [H]

Synonym: XfasM23_1287

Alternate gene names: 182681822

Gene position: 1418571-1419203 (Clockwise)

Preceding gene: 182681821

Following gene: 182681823

Centisome position: 55.94

GC content: 50.55

Gene sequence:

>633_bases
ATGAACCAATCCATCACACCACCTACACACCCGCCAATGTCCCAACGCTTCCGCGGCTATCTGCCTGTAGTGGTCGACGT
TGAAACGGGAGGCTTTAACTGGAACCACCACGCCCTGCTAGAGATCGCCTGTATCCCCATTGAAATGGACGATACTGGAA
GGCTCTACCCCGGAGAAGTCACCAGCACCCATGTCATTCCAGCACCTGGTACCGACATCGATCCCAAATCACTGGAAGTC
ACTGGGATCATACTCGACCATCCGTTTCGCTTCGCCAAGGAAGAAAAGCTAGCGTTGGAACATATATTCACACCAGTGCG
CACCACAATGAAGAAATACAAATGCCAGCGCGCAATCTTGGTAGGACACAATGCGCATTTTGACCTCAATTTCCTCAACG
CAGCAGTAACGCGAACCGCATACAAACGAAATCCATTTCATCAATTCAGCGTATTTGACACTGTCACTCTAGCAGGCATG
GCCTACGGTCAAACCGTATTAGCACGTGCAGTACAAGCCGCCGGCTTGGATTGGAACGCAGCTGACGCACACAGCGCAGT
CTACGATGCTGAAAAAACAGCTCACCTATTCTGCACAATCACCAACATATGGCCATTAATGGTTGCTGGATGA

Upstream 100 bases:

>100_bases
ACCAGCTAATCTGATCGCAATCCTCATTTTCATCGGTTATATCTCTCAGCAACCTCAGGTGCTCCAACCTGATCGAATGC
TGTCCTGCTAGGCTATCGTG

Downstream 100 bases:

>100_bases
TTTCTTTTTCCTCTCCTACCTACCTTCTGTATGTCTTCAGACTAATACACCAACACAACAAAACACTCACTTAGACACGC
CCTCATTCCTGGCAGTAAAA

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MNQSITPPTHPPMSQRFRGYLPVVVDVETGGFNWNHHALLEIACIPIEMDDTGRLYPGEVTSTHVIPAPGTDIDPKSLEV
TGIILDHPFRFAKEEKLALEHIFTPVRTTMKKYKCQRAILVGHNAHFDLNFLNAAVTRTAYKRNPFHQFSVFDTVTLAGM
AYGQTVLARAVQAAGLDWNAADAHSAVYDAEKTAHLFCTITNIWPLMVAG

Sequences:

>Translated_210_residues
MNQSITPPTHPPMSQRFRGYLPVVVDVETGGFNWNHHALLEIACIPIEMDDTGRLYPGEVTSTHVIPAPGTDIDPKSLEV
TGIILDHPFRFAKEEKLALEHIFTPVRTTMKKYKCQRAILVGHNAHFDLNFLNAAVTRTAYKRNPFHQFSVFDTVTLAGM
AYGQTVLARAVQAAGLDWNAADAHSAVYDAEKTAHLFCTITNIWPLMVAG
>Mature_210_residues
MNQSITPPTHPPMSQRFRGYLPVVVDVETGGFNWNHHALLEIACIPIEMDDTGRLYPGEVTSTHVIPAPGTDIDPKSLEV
TGIILDHPFRFAKEEKLALEHIFTPVRTTMKKYKCQRAILVGHNAHFDLNFLNAAVTRTAYKRNPFHQFSVFDTVTLAGM
AYGQTVLARAVQAAGLDWNAADAHSAVYDAEKTAHLFCTITNIWPLMVAG

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=192, Percent_Identity=51.0416666666667, Blast_Score=196, Evalue=8e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.-

Molecular weight: Translated: 23312; Mature: 23312

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQSITPPTHPPMSQRFRGYLPVVVDVETGGFNWNHHALLEIACIPIEMDDTGRLYPGEV
CCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCEEEEEEEEEEEEECCCCCCCCCCC
TSTHVIPAPGTDIDPKSLEVTGIILDHPFRFAKEEKLALEHIFTPVRTTMKKYKCQRAIL
CCCEEECCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEE
VGHNAHFDLNFLNAAVTRTAYKRNPFHQFSVFDTVTLAGMAYGQTVLARAVQAAGLDWNA
EECCCEEEHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ADAHSAVYDAEKTAHLFCTITNIWPLMVAG
CCCHHHHHCCCCCEEEEEEECCCCEEEEEC
>Mature Secondary Structure
MNQSITPPTHPPMSQRFRGYLPVVVDVETGGFNWNHHALLEIACIPIEMDDTGRLYPGEV
CCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCEEEEEEEEEEEEECCCCCCCCCCC
TSTHVIPAPGTDIDPKSLEVTGIILDHPFRFAKEEKLALEHIFTPVRTTMKKYKCQRAIL
CCCEEECCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEE
VGHNAHFDLNFLNAAVTRTAYKRNPFHQFSVFDTVTLAGMAYGQTVLARAVQAAGLDWNA
EECCCEEEHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ADAHSAVYDAEKTAHLFCTITNIWPLMVAG
CCCHHHHHCCCCCEEEEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]