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The map label for this gene is ksgA

Identifier: 182681823

GI number: 182681823

Start: 1419589

End: 1420482

Strand: Direct

Name: ksgA

Synonym: XfasM23_1288

Alternate gene names: 182681823

Gene position: 1419589-1420482 (Clockwise)

Preceding gene: 182681822

Following gene: 182681824

Centisome position: 55.98

GC content: 53.69

Gene sequence:

>894_bases
ATGATGCAACCTCCCATTAACGTGCATACGCCACACTCCAGCGCACAATTGACACTGCTGCCGCCCAAACACCTCAATCA
ATTAGAATCCCATGGCATGGAATCTCGACTGTTCAATACACCAGCGAAAAAAGCGTTCGGCCAACATTTTCTTGTTGACC
GTTACTACATCGACCGCATCATCCATGCCATCAACCCTCAGCCCAACGACCACATCGTCGAAATCGGCCCCGGTCAGGGG
GCAATCACCCTGCCACTGCTGAAATGCTGCGGCTCCCTGACCGCAATTGAGTTGGACCGCGACCTGATCGCTCCGCTGAC
CGCTGCTGCTACGCCTATAGGCAAGCTAGACATCATCCACCGCGACGTACTCACAGTAGACCTCTCCATCCTAGCCAAAC
AAGGAAATAAGAAATTACGCCTGGTCGGCAACCTCCCCTACAACATCTCCTCGCCGATCCTTTTCCATGTACTCCAGCAA
GCTGCAATCATCGCCGACATGCACTTCATGTTGCAGAAAGAAGTCGTCGACCGCATGGCAGCACCTCCAGGTAGCAAGGT
CTACGGTCGGCTCAGTGTCATGCTACAGGCTTGGTGTGAGGTCACGACCATGTTCGTCGTGCCGCCTGACGCCTTCCAAC
CACCACCGAAGGTAAACTCAGCAATCACCAGACTCGTGCCACGCGATCCAACAACAATCCGTATTGCTGATACAAAGCGT
TTCAGCGACATCGTTCGTGCCGCATTTGGTCAACGTCGCAAGACACTGCGTAATTCCCTAGCCGACATATGCACACCGGC
TCACTTCGAACATGCCGGGATACGCACTAACGCACGTGCCGAACAACTGGAAGTCACCGAATTCATCGCACTCGCCAATG
CCAAGAACACTTAA

Upstream 100 bases:

>100_bases
ACAAATAGTGCAGCACCATTATTGATGGTAATAAATCATACATACTTAATCTGTTTCTATTAACTAATGTATATAAATAA
GTGCTTCCATCAATTCTAAA

Downstream 100 bases:

>100_bases
ACGGCATTCATACGCTACTTTGCTTACACTGCACACATGGAAAACAATCCCAGTTCAAAGATTGAGGTCGCTGTCTCGTC
CCGCTTCCTCGACCAGCAAT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MMQPPINVHTPHSSAQLTLLPPKHLNQLESHGMESRLFNTPAKKAFGQHFLVDRYYIDRIIHAINPQPNDHIVEIGPGQG
AITLPLLKCCGSLTAIELDRDLIAPLTAAATPIGKLDIIHRDVLTVDLSILAKQGNKKLRLVGNLPYNISSPILFHVLQQ
AAIIADMHFMLQKEVVDRMAAPPGSKVYGRLSVMLQAWCEVTTMFVVPPDAFQPPPKVNSAITRLVPRDPTTIRIADTKR
FSDIVRAAFGQRRKTLRNSLADICTPAHFEHAGIRTNARAEQLEVTEFIALANAKNT

Sequences:

>Translated_297_residues
MMQPPINVHTPHSSAQLTLLPPKHLNQLESHGMESRLFNTPAKKAFGQHFLVDRYYIDRIIHAINPQPNDHIVEIGPGQG
AITLPLLKCCGSLTAIELDRDLIAPLTAAATPIGKLDIIHRDVLTVDLSILAKQGNKKLRLVGNLPYNISSPILFHVLQQ
AAIIADMHFMLQKEVVDRMAAPPGSKVYGRLSVMLQAWCEVTTMFVVPPDAFQPPPKVNSAITRLVPRDPTTIRIADTKR
FSDIVRAAFGQRRKTLRNSLADICTPAHFEHAGIRTNARAEQLEVTEFIALANAKNT
>Mature_297_residues
MMQPPINVHTPHSSAQLTLLPPKHLNQLESHGMESRLFNTPAKKAFGQHFLVDRYYIDRIIHAINPQPNDHIVEIGPGQG
AITLPLLKCCGSLTAIELDRDLIAPLTAAATPIGKLDIIHRDVLTVDLSILAKQGNKKLRLVGNLPYNISSPILFHVLQQ
AAIIADMHFMLQKEVVDRMAAPPGSKVYGRLSVMLQAWCEVTTMFVVPPDAFQPPPKVNSAITRLVPRDPTTIRIADTKR
FSDIVRAAFGQRRKTLRNSLADICTPAHFEHAGIRTNARAEQLEVTEFIALANAKNT

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657198, Length=223, Percent_Identity=34.9775784753363, Blast_Score=118, Evalue=5e-27,
Organism=Homo sapiens, GI156415992, Length=285, Percent_Identity=30.1754385964912, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1786236, Length=252, Percent_Identity=48.4126984126984, Blast_Score=235, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI25146882, Length=230, Percent_Identity=31.304347826087, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI25141369, Length=247, Percent_Identity=25.9109311740891, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324989, Length=231, Percent_Identity=30.3030303030303, Blast_Score=105, Evalue=7e-24,
Organism=Drosophila melanogaster, GI21358017, Length=234, Percent_Identity=32.0512820512821, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21357273, Length=262, Percent_Identity=30.1526717557252, Blast_Score=111, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 32864; Mature: 32864

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQPPINVHTPHSSAQLTLLPPKHLNQLESHGMESRLFNTPAKKAFGQHFLVDRYYIDRI
CCCCCCCEECCCCCCEEEEECHHHHHHHHHCCCHHHHHCCCHHHHHCHHHHHHHHHHHHH
IHAINPQPNDHIVEIGPGQGAITLPLLKCCGSLTAIELDRDLIAPLTAAATPIGKLDIIH
HHHCCCCCCCCEEEECCCCCCCHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCHHHHHH
RDVLTVDLSILAKQGNKKLRLVGNLPYNISSPILFHVLQQAAIIADMHFMLQKEVVDRMA
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
APPGSKVYGRLSVMLQAWCEVTTMFVVPPDAFQPPPKVNSAITRLVPRDPTTIRIADTKR
CCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEECCHH
FSDIVRAAFGQRRKTLRNSLADICTPAHFEHAGIRTNARAEQLEVTEFIALANAKNT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMQPPINVHTPHSSAQLTLLPPKHLNQLESHGMESRLFNTPAKKAFGQHFLVDRYYIDRI
CCCCCCCEECCCCCCEEEEECHHHHHHHHHCCCHHHHHCCCHHHHHCHHHHHHHHHHHHH
IHAINPQPNDHIVEIGPGQGAITLPLLKCCGSLTAIELDRDLIAPLTAAATPIGKLDIIH
HHHCCCCCCCCEEEECCCCCCCHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCHHHHHH
RDVLTVDLSILAKQGNKKLRLVGNLPYNISSPILFHVLQQAAIIADMHFMLQKEVVDRMA
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
APPGSKVYGRLSVMLQAWCEVTTMFVVPPDAFQPPPKVNSAITRLVPRDPTTIRIADTKR
CCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEECCHH
FSDIVRAAFGQRRKTLRNSLADICTPAHFEHAGIRTNARAEQLEVTEFIALANAKNT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]