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The map label for this gene is tolB

Identifier: 182681495

GI number: 182681495

Start: 1113990

End: 1115414

Strand: Direct

Name: tolB

Synonym: XfasM23_0949

Alternate gene names: 182681495

Gene position: 1113990-1115414 (Clockwise)

Preceding gene: 182681494

Following gene: 182681496

Centisome position: 43.93

GC content: 48.42

Gene sequence:

>1425_bases
ATGGCTGCGTTCACGCTTTTGTTGTTAACGATTGCTAAAATATTCGTTCTGGGGCATCCGTTTAAATTTTCATCCATTAT
TTTTCTTAAAGTTATAGATCTACACATGACGAAATTTCCACGCTGGCTGGCCATGTTGGTCGGTTTATTGTTCCCCTTAT
CAGCTCTGACGCAGCAACAGGGGTTGACGATCGATATCGTTGGCGGCAACACCGCAGCGACTCCTATTGCAGTTTTACCT
ATGCCTTATCATGACAGTGCAGGTGCTCCAGCTACGGATGTTTCGGGTGTTGTTGCTGCTGACTTGAACCGTTCAGGTCA
GTTCCGTACGTTGCCTCTAGGGCAAATTACTGAGCGCCCTACTCATGGAAGCGAGATTCGATTTCCGACTTGGCAAGCAC
TGAAACAGGACTACATCGTGGTTGGTCGTGTGCTTGATGCCAGGCAAGGGACTTACCGTGTCGAGTATGAACTATTCGAT
GTTCGCAACGGCAAACGAATGCTTGGATTGGCGATGACTGCACGCGCTAGCGCGATGCGTGACGTTGCACACCAAATGGC
CGATGCAATCTACGAAAAAATTACCGGTTTGCGCGGTGCATTCTTTACCCGCATTGCTTATGTCACTGCCAGTGGTTCAC
ACGGAGCAATGCGGTACGCACTGATGGTTGCTGACTCAGACGGCTACAATCCACAAACCATCGTGCGTTCAGCCGAACCA
CTGTTATCACCTGATTGGAGCTCTGATGGTAAGAAACTGGCGTACGTTAGTTTTGAGAAAGGGGGGTCTTCAATCTACAT
TCAAGACATTGCTACAGGTTCCCGTGAATTGGTGTCAAGCTTCCGGGGTATCAATGCTGCACCATCGTTTGCTCCCGATG
GCCATCGAATCGCTTTATCTCTGTCAAGAAGTGGTAATCCTGAAATCTACGTCATGGACTTGGTTAGCAAACAGTTAATT
CAGTTGACCAATAGTTTCGGAATTGATACTGAACCGGTTTGGTCGTCAGATGGGAAATTTATTTATTTCACTTCTGACCG
CGGTGGGCGTCCACAGATATATAAAGTCGCCTCTGTTGGAGGCGCAGCGACACGTGTTACATTTCAAGGCAACTACAATG
CTAGTGCTAGCGTCTCCTATGACGACAAGAAGATTGTCGTAGCACAAGGGAGCGGTAACGTATACCGAATTGCAATGATG
GACCAAAGTTCGGGATCTACGGTTTGGAACACTTTATCTACTGGTTCATTGGATGAATCTCCGAGCTTTGCTCCCAATGC
AAGTATGGTCCTTTATGCAGCACGTGAAGGAGGCCGTGGAGTACTTTATGCAGTCTCTGCGGATGCGCGTGTGCGCCAGC
GCCTAGTATCGGTTGATAGTGATGTAAGAGAGCCTGCTTGGGGGCCTTACCGGAGCGTACATTAA

Upstream 100 bases:

>100_bases
TATAAACAAGATACTGAGATATGTAGTAACGATATTCTTTAGCTAGTGACTGACTTTGATCTCTACTTGTCGCGTTTGCA
CATCTTGGTCATCATTTCCA

Downstream 100 bases:

>100_bases
TAAATAATGTTAATATTTTGATGTGTTAACCACTCATTGGCTTGGGAGCTACCATAGGTATCTTTATGAATAATTCCACA
CGTTTTCTCCTAATCTCATT

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MAAFTLLLLTIAKIFVLGHPFKFSSIIFLKVIDLHMTKFPRWLAMLVGLLFPLSALTQQQGLTIDIVGGNTAATPIAVLP
MPYHDSAGAPATDVSGVVAADLNRSGQFRTLPLGQITERPTHGSEIRFPTWQALKQDYIVVGRVLDARQGTYRVEYELFD
VRNGKRMLGLAMTARASAMRDVAHQMADAIYEKITGLRGAFFTRIAYVTASGSHGAMRYALMVADSDGYNPQTIVRSAEP
LLSPDWSSDGKKLAYVSFEKGGSSIYIQDIATGSRELVSSFRGINAAPSFAPDGHRIALSLSRSGNPEIYVMDLVSKQLI
QLTNSFGIDTEPVWSSDGKFIYFTSDRGGRPQIYKVASVGGAATRVTFQGNYNASASVSYDDKKIVVAQGSGNVYRIAMM
DQSSGSTVWNTLSTGSLDESPSFAPNASMVLYAAREGGRGVLYAVSADARVRQRLVSVDSDVREPAWGPYRSVH

Sequences:

>Translated_474_residues
MAAFTLLLLTIAKIFVLGHPFKFSSIIFLKVIDLHMTKFPRWLAMLVGLLFPLSALTQQQGLTIDIVGGNTAATPIAVLP
MPYHDSAGAPATDVSGVVAADLNRSGQFRTLPLGQITERPTHGSEIRFPTWQALKQDYIVVGRVLDARQGTYRVEYELFD
VRNGKRMLGLAMTARASAMRDVAHQMADAIYEKITGLRGAFFTRIAYVTASGSHGAMRYALMVADSDGYNPQTIVRSAEP
LLSPDWSSDGKKLAYVSFEKGGSSIYIQDIATGSRELVSSFRGINAAPSFAPDGHRIALSLSRSGNPEIYVMDLVSKQLI
QLTNSFGIDTEPVWSSDGKFIYFTSDRGGRPQIYKVASVGGAATRVTFQGNYNASASVSYDDKKIVVAQGSGNVYRIAMM
DQSSGSTVWNTLSTGSLDESPSFAPNASMVLYAAREGGRGVLYAVSADARVRQRLVSVDSDVREPAWGPYRSVH
>Mature_473_residues
AAFTLLLLTIAKIFVLGHPFKFSSIIFLKVIDLHMTKFPRWLAMLVGLLFPLSALTQQQGLTIDIVGGNTAATPIAVLPM
PYHDSAGAPATDVSGVVAADLNRSGQFRTLPLGQITERPTHGSEIRFPTWQALKQDYIVVGRVLDARQGTYRVEYELFDV
RNGKRMLGLAMTARASAMRDVAHQMADAIYEKITGLRGAFFTRIAYVTASGSHGAMRYALMVADSDGYNPQTIVRSAEPL
LSPDWSSDGKKLAYVSFEKGGSSIYIQDIATGSRELVSSFRGINAAPSFAPDGHRIALSLSRSGNPEIYVMDLVSKQLIQ
LTNSFGIDTEPVWSSDGKFIYFTSDRGGRPQIYKVASVGGAATRVTFQGNYNASASVSYDDKKIVVAQGSGNVYRIAMMD
QSSGSTVWNTLSTGSLDESPSFAPNASMVLYAAREGGRGVLYAVSADARVRQRLVSVDSDVREPAWGPYRSVH

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=409, Percent_Identity=41.8092909535452, Blast_Score=312, Evalue=3e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 51427; Mature: 51296

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAFTLLLLTIAKIFVLGHPFKFSSIIFLKVIDLHMTKFPRWLAMLVGLLFPLSALTQQQ
CHHHHHHHHHHHHHHHHCCCCCHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GLTIDIVGGNTAATPIAVLPMPYHDSAGAPATDVSGVVAADLNRSGQFRTLPLGQITERP
CCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCEEEECCHHHCCCC
THGSEIRFPTWQALKQDYIVVGRVLDARQGTYRVEYELFDVRNGKRMLGLAMTARASAMR
CCCCEECCCCHHHHHCCEEEEEEEECCCCCCEEEEEEEEECCCCCEEEEEEHHHHHHHHH
DVAHQMADAIYEKITGLRGAFFTRIAYVTASGSHGAMRYALMVADSDGYNPQTIVRSAEP
HHHHHHHHHHHHHHHCCCHHHEEEEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHCCCC
LLSPDWSSDGKKLAYVSFEKGGSSIYIQDIATGSRELVSSFRGINAAPSFAPDGHRIALS
CCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCEEEEE
LSRSGNPEIYVMDLVSKQLIQLTNSFGIDTEPVWSSDGKFIYFTSDRGGRPQIYKVASVG
EECCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECC
GAATRVTFQGNYNASASVSYDDKKIVVAQGSGNVYRIAMMDQSSGSTVWNTLSTGSLDES
CCEEEEEEECCCCCCEEEECCCCEEEEEECCCCEEEEEEEECCCCCEEEEECCCCCCCCC
PSFAPNASMVLYAAREGGRGVLYAVSADARVRQRLVSVDSDVREPAWGPYRSVH
CCCCCCCCEEEEEECCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AAFTLLLLTIAKIFVLGHPFKFSSIIFLKVIDLHMTKFPRWLAMLVGLLFPLSALTQQQ
HHHHHHHHHHHHHHHHCCCCCHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GLTIDIVGGNTAATPIAVLPMPYHDSAGAPATDVSGVVAADLNRSGQFRTLPLGQITERP
CCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCEEEECCHHHCCCC
THGSEIRFPTWQALKQDYIVVGRVLDARQGTYRVEYELFDVRNGKRMLGLAMTARASAMR
CCCCEECCCCHHHHHCCEEEEEEEECCCCCCEEEEEEEEECCCCCEEEEEEHHHHHHHHH
DVAHQMADAIYEKITGLRGAFFTRIAYVTASGSHGAMRYALMVADSDGYNPQTIVRSAEP
HHHHHHHHHHHHHHHCCCHHHEEEEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHCCCC
LLSPDWSSDGKKLAYVSFEKGGSSIYIQDIATGSRELVSSFRGINAAPSFAPDGHRIALS
CCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCEEEEE
LSRSGNPEIYVMDLVSKQLIQLTNSFGIDTEPVWSSDGKFIYFTSDRGGRPQIYKVASVG
EECCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECC
GAATRVTFQGNYNASASVSYDDKKIVVAQGSGNVYRIAMMDQSSGSTVWNTLSTGSLDES
CCEEEEEEECCCCCCEEEECCCCEEEEEECCCCEEEEEEEECCCCCEEEEECCCCCCCCC
PSFAPNASMVLYAAREGGRGVLYAVSADARVRQRLVSVDSDVREPAWGPYRSVH
CCCCCCCCEEEEEECCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]