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The map label for this gene is nadB [H]

Identifier: 182681469

GI number: 182681469

Start: 1089384

End: 1090922

Strand: Reverse

Name: nadB [H]

Synonym: XfasM23_0921

Alternate gene names: 182681469

Gene position: 1090922-1089384 (Counterclockwise)

Preceding gene: 182681470

Following gene: 182681468

Centisome position: 43.02

GC content: 55.43

Gene sequence:

>1539_bases
ATGGCTAAGATAGTGGACTGTTTTGCAGACCGCCCCATCATCGTTGGTAGCGGGCTTGCTGGTCTGATCGCAGCACTGAC
CATTTCTCCTGAACCCTCAGTGCTTGTCACGCGCAGCGCCTTGGGCGCAGAAACATCAAGCGCATGGGCTCAGGGAGGAA
TGTCGGTCAGCCTTAGCCCAGATGACAACCCCAGCCTGCATCTTGCCGACACCTTGGCGGCTGGCGATGGTCTGTGTGAT
GCAGTCGCTGCAGAGAGCATCATCTTGGAAGCGCTTGACGCTTTTCATGTCCTACAACACTTTGGCATCCACTTCGACCA
AGATAGCAACGGCCATCTGGCGCTTGGACTGGAAGCAGCCCATTCCCGTCGCCGTATCGTGCATGTTGGGGGCGATAGAT
CGGGTACAGCGATTGTCCAAGCATTGACTGCGTGTGTTCTCAACGATCCCTCAATCACCGTGCTTGAGGGCCTCGAAGCA
CGACACATTCTGATGGCTGATAACGTGGTCTGTGGCTTGCTGCTTGCCAACCCGACATCCGAGATTGTGTTGCCTTCGTC
AAGAATTCTTCTAGCAACAGGCGGCATTGGCGGGCTTTATGATGCGACTACCAACCCAGTGAACAACTTTGGTCAAGGTA
TCGCGATGGCTGCACGTGCTGGCGCCGTGCTGGCTGATATGGAATTCGTACAGTTCCATCCGACGGCCCTCCATTGCCAC
AACCGCCCGCTGGCGCTCGTCAGTGAAGCTGTCCGTGGTGAAGGTGCAGTCCTCATTAACGAACGTGGCGAACGCTTTAT
GGCGGATATTCCTGGTGCCGAACTTGCAGCTCGTAACATCGTTGCACAGGCAATCAGCGCCGAAATAACCCGCGGCGGCC
AAGTATTTCTCGATGCCAGGCAAGCATTGGGAGCACGCTTCAGCACCCGCTTTCCAACCATCACCGCACTCTGCCACAAA
GTAGGTATTGACCCTGTACACATGCCGATTCCAGTCTGTCCCGCTGCTCATTACCACATGGGCGGTATCGCAACCGATCG
CCAAGGCCGCAGCAGCATTCCTGGCTTATGGGTTGCGGGTGAAGCAGCGTCTACAGGCTTACATGGAGCAAATCGGCTTG
CGAGCAATTCACTGCTTGAGGCCGTCGTCATGGGGACACGTGCAGCCCGAGATATCACATCCCACAACACACCCCACCCC
CTCGGAACAATCCCTATCACCCCTAAAAAGCCTGATACCACACTGATCCGGCCGATTGTCTCTCAGTGCCTTGGCGTACT
TCGCCATGCCGCGGATATGCATCGTGCTATTGCTGCCCTCTTGCCTTTTGTGGAAGGCGAAGAAGAATCTTCAGATCCCG
CCATCGTTGCATTGCTGATCGCCATCTTTGCACATCTTCGCACCGAATCACGCGGTGCCCATGCACGCACCGATTTCCCT
CTCAAACATGACACAACACAGCGGCGCAACATGACACTTGAGGATGTTCTTGAAATCGCCCACTCCCGTATTGCACAACG
CAAAGAAAAGATCTCCTGA

Upstream 100 bases:

>100_bases
CTTGGAAGAAAACCGTCACGAAGTCACGGTAGATGCAAAAATTGCAGACCCCGCACGACGCGCCGTTGAAAGGATGTTGG
CGATATGACCAACGAGCAAG

Downstream 100 bases:

>100_bases
CATGAACCTCGTTTCTCTTCCACGTATCATCATTGAACCGATGGTACGCCATGCGCTTGCAGAAGATTTAGGACTTGCAG
GCGACATTACCTCAGCCGCA

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MAKIVDCFADRPIIVGSGLAGLIAALTISPEPSVLVTRSALGAETSSAWAQGGMSVSLSPDDNPSLHLADTLAAGDGLCD
AVAAESIILEALDAFHVLQHFGIHFDQDSNGHLALGLEAAHSRRRIVHVGGDRSGTAIVQALTACVLNDPSITVLEGLEA
RHILMADNVVCGLLLANPTSEIVLPSSRILLATGGIGGLYDATTNPVNNFGQGIAMAARAGAVLADMEFVQFHPTALHCH
NRPLALVSEAVRGEGAVLINERGERFMADIPGAELAARNIVAQAISAEITRGGQVFLDARQALGARFSTRFPTITALCHK
VGIDPVHMPIPVCPAAHYHMGGIATDRQGRSSIPGLWVAGEAASTGLHGANRLASNSLLEAVVMGTRAARDITSHNTPHP
LGTIPITPKKPDTTLIRPIVSQCLGVLRHAADMHRAIAALLPFVEGEEESSDPAIVALLIAIFAHLRTESRGAHARTDFP
LKHDTTQRRNMTLEDVLEIAHSRIAQRKEKIS

Sequences:

>Translated_512_residues
MAKIVDCFADRPIIVGSGLAGLIAALTISPEPSVLVTRSALGAETSSAWAQGGMSVSLSPDDNPSLHLADTLAAGDGLCD
AVAAESIILEALDAFHVLQHFGIHFDQDSNGHLALGLEAAHSRRRIVHVGGDRSGTAIVQALTACVLNDPSITVLEGLEA
RHILMADNVVCGLLLANPTSEIVLPSSRILLATGGIGGLYDATTNPVNNFGQGIAMAARAGAVLADMEFVQFHPTALHCH
NRPLALVSEAVRGEGAVLINERGERFMADIPGAELAARNIVAQAISAEITRGGQVFLDARQALGARFSTRFPTITALCHK
VGIDPVHMPIPVCPAAHYHMGGIATDRQGRSSIPGLWVAGEAASTGLHGANRLASNSLLEAVVMGTRAARDITSHNTPHP
LGTIPITPKKPDTTLIRPIVSQCLGVLRHAADMHRAIAALLPFVEGEEESSDPAIVALLIAIFAHLRTESRGAHARTDFP
LKHDTTQRRNMTLEDVLEIAHSRIAQRKEKIS
>Mature_511_residues
AKIVDCFADRPIIVGSGLAGLIAALTISPEPSVLVTRSALGAETSSAWAQGGMSVSLSPDDNPSLHLADTLAAGDGLCDA
VAAESIILEALDAFHVLQHFGIHFDQDSNGHLALGLEAAHSRRRIVHVGGDRSGTAIVQALTACVLNDPSITVLEGLEAR
HILMADNVVCGLLLANPTSEIVLPSSRILLATGGIGGLYDATTNPVNNFGQGIAMAARAGAVLADMEFVQFHPTALHCHN
RPLALVSEAVRGEGAVLINERGERFMADIPGAELAARNIVAQAISAEITRGGQVFLDARQALGARFSTRFPTITALCHKV
GIDPVHMPIPVCPAAHYHMGGIATDRQGRSSIPGLWVAGEAASTGLHGANRLASNSLLEAVVMGTRAARDITSHNTPHPL
GTIPITPKKPDTTLIRPIVSQCLGVLRHAADMHRAIAALLPFVEGEEESSDPAIVALLIAIFAHLRTESRGAHARTDFPL
KHDTTQRRNMTLEDVLEIAHSRIAQRKEKIS

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=508, Percent_Identity=29.5275590551181, Blast_Score=175, Evalue=1e-43,
Organism=Escherichia coli, GI1788928, Length=510, Percent_Identity=36.6666666666667, Blast_Score=264, Evalue=1e-71,
Organism=Escherichia coli, GI1786942, Length=512, Percent_Identity=31.640625, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1790597, Length=369, Percent_Identity=33.6043360433604, Blast_Score=177, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17505833, Length=518, Percent_Identity=30.5019305019305, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17550100, Length=510, Percent_Identity=30.3921568627451, Blast_Score=175, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI71986328, Length=436, Percent_Identity=28.4403669724771, Blast_Score=92, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6322701, Length=508, Percent_Identity=30.511811023622, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6322416, Length=514, Percent_Identity=30.1556420233463, Blast_Score=170, Evalue=6e-43,
Organism=Drosophila melanogaster, GI17137288, Length=508, Percent_Identity=29.7244094488189, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24655642, Length=508, Percent_Identity=29.7244094488189, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24655647, Length=508, Percent_Identity=29.7244094488189, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24663005, Length=508, Percent_Identity=29.5275590551181, Blast_Score=155, Evalue=8e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 53938; Mature: 53807

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIVDCFADRPIIVGSGLAGLIAALTISPEPSVLVTRSALGAETSSAWAQGGMSVSLSP
CCCHHHHHCCCCEEEECCHHHHHHEEEECCCCCEEEEEHHCCCCCHHHHHHCCCEEEECC
DDNPSLHLADTLAAGDGLCDAVAAESIILEALDAFHVLQHFGIHFDQDSNGHLALGLEAA
CCCCCEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEEEHHC
HSRRRIVHVGGDRSGTAIVQALTACVLNDPSITVLEGLEARHILMADNVVCGLLLANPTS
CCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEECCCEEEEEEECCCC
EIVLPSSRILLATGGIGGLYDATTNPVNNFGQGIAMAARAGAVLADMEFVQFHPTALHCH
CEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCHHHEECCCEEEEC
NRPLALVSEAVRGEGAVLINERGERFMADIPGAELAARNIVAQAISAEITRGGQVFLDAR
CCCHHHHHHHHCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHH
QALGARFSTRFPTITALCHKVGIDPVHMPIPVCPAAHYHMGGIATDRQGRSSIPGLWVAG
HHHCCHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEC
EAASTGLHGANRLASNSLLEAVVMGTRAARDITSHNTPHPLGTIPITPKKPDTTLIRPIV
CHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCHHHHHHHH
SQCLGVLRHAADMHRAIAALLPFVEGEEESSDPAIVALLIAIFAHLRTESRGAHARTDFP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
LKHDTTQRRNMTLEDVLEIAHSRIAQRKEKIS
CCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKIVDCFADRPIIVGSGLAGLIAALTISPEPSVLVTRSALGAETSSAWAQGGMSVSLSP
CCHHHHHCCCCEEEECCHHHHHHEEEECCCCCEEEEEHHCCCCCHHHHHHCCCEEEECC
DDNPSLHLADTLAAGDGLCDAVAAESIILEALDAFHVLQHFGIHFDQDSNGHLALGLEAA
CCCCCEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEEEHHC
HSRRRIVHVGGDRSGTAIVQALTACVLNDPSITVLEGLEARHILMADNVVCGLLLANPTS
CCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEECCCEEEEEEECCCC
EIVLPSSRILLATGGIGGLYDATTNPVNNFGQGIAMAARAGAVLADMEFVQFHPTALHCH
CEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCHHHEECCCEEEEC
NRPLALVSEAVRGEGAVLINERGERFMADIPGAELAARNIVAQAISAEITRGGQVFLDAR
CCCHHHHHHHHCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHH
QALGARFSTRFPTITALCHKVGIDPVHMPIPVCPAAHYHMGGIATDRQGRSSIPGLWVAG
HHHCCHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEC
EAASTGLHGANRLASNSLLEAVVMGTRAARDITSHNTPHPLGTIPITPKKPDTTLIRPIV
CHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCHHHHHHHH
SQCLGVLRHAADMHRAIAALLPFVEGEEESSDPAIVALLIAIFAHLRTESRGAHARTDFP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
LKHDTTQRRNMTLEDVLEIAHSRIAQRKEKIS
CCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]