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The map label for this gene is purF [H]

Identifier: 182681448

GI number: 182681448

Start: 1067976

End: 1069433

Strand: Reverse

Name: purF [H]

Synonym: XfasM23_0899

Alternate gene names: 182681448

Gene position: 1069433-1067976 (Counterclockwise)

Preceding gene: 182681449

Following gene: 182681441

Centisome position: 42.18

GC content: 48.97

Gene sequence:

>1458_bases
ATGTGCGGTATCCTCGGTATCGTTGGCAATCAAAACGTTGCTGGCCAACTCTATGACGGTTTGACTGTTTTGCAACATCG
TGGACAAGATGCTGCAGGCATTGCCACGGCCAGTGGCACTCGCTTGCGCGTTCAAAAGGCCAATGGTTTGGTACGAGACG
TATTCGATGAGCAGCGTATGGCGGTCCTTGATGGGTGTATTGGGATTGCTCATTGCCGTTATCCAACTGCTGGTTCTGAG
GGGATGGATGAGGCACAACCTTTTTACGTCAACTCACCGTACGGCATTGCTCTAGCACATAATGGAAACCTTATCAACAC
AGAGGCGCTACACCAACAGGTGTTCGAAGCGGATCGACGTAACATCAACACCGACTCTGACAGCGAAGTATTACTTAACG
TATTCGCATATGAGTTAGATGCGCAGCGTATGCTCACTCCAGAAGCGGCAATTCGTGCTGTAGCAGGAGTACATCGCCGC
TGTAAGGGCGGCTACGCCGTAGTGAGCATGATATTAGGGCTGGGACTTGTTGCATTCCGCGATCCACACGGTATCCGTCC
GTTGGTGCTGGGAAAGCGTGTACATACCGAAGGTGATGAATATATGGTCGCTTCCGAATCAGCTGCACTCGATATCCTAG
GGTTTATACGTATACGCGATGTACGTCCAGGTGAGGCTGTGGTGATTACTGCACGTGGCGAACTGTTTTCAGAGGTCTGT
GCTACACCTACCAACCATACTCCATGCATTTTCGAGTACGTTTACTTCGCACGTCCGGATTCGATGATCGATAATATTTC
AGTACATAAGGCACGCATGCGTATGGGTGTGAAGTTAGGTGAAAAGATCCTGCGCTTGCGCCCGAATCACGATATCGACA
CCATTATTCCGATACCGGATACCTCGCGGGATGCAGCGCTGGAAATATCTAACGTGCTCGGCGTAAAGTATCGCGAAGGT
TTCGTTAAAAATCGCTACGTTGGCCGTACTTTTATCATGCCGGGACAGGGAGAGCGAGTTAAGTCAGTACGTCGTAAACT
TAACCCAATCCATCTGGAGTTCCGAAACCGCGTGGTGCTGTTGATTGATGATTCAATTGTTCGCGGTACCACCAGCCGCC
AGATTGTCCAGATGGTAAGAGATGCTGGTGCACGCAAGGTGTACTTAGCAAGTGCTGCGCCACCTGTGCGTTATCCAAAT
GTGTATGGCATTGATATGCCAGCTGCAGATGAATTAATCGCACATGATCGGACTGAGCAGGAAATACAATCCCTTCTTGG
CTGCGATTGGTTGATTTACCAAGATTTAGAGGATCTAGAAAACGCTGTGCGTGAGGGAAACCAAGAAATCAAGCAATTTG
ATTCCTCATGCTTCAATGGTCATTACATCACTGGAATTGAACGGGGCTATTTAGATCGCATCCGTCAACTACGCTCCGAT
GAGGTAAAGCGAAAATAA

Upstream 100 bases:

>100_bases
CTGGAGAACATAATCTCCTGAAAATCTAAAGTTCACGGCCCAGCACCTACAAACTCACAGTAATGCAGTCCCAAGAAGCT
GCAGCGGAGAACTCTCAATC

Downstream 100 bases:

>100_bases
TACAATTAATATATTTTTCGATAATAAAATTAGTCTAAAGCTAATTACCTATCTCAAAAAAAGCGGCGAACCCAGCCCCC
CGGCATGGATCCACCGCAAA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MCGILGIVGNQNVAGQLYDGLTVLQHRGQDAAGIATASGTRLRVQKANGLVRDVFDEQRMAVLDGCIGIAHCRYPTAGSE
GMDEAQPFYVNSPYGIALAHNGNLINTEALHQQVFEADRRNINTDSDSEVLLNVFAYELDAQRMLTPEAAIRAVAGVHRR
CKGGYAVVSMILGLGLVAFRDPHGIRPLVLGKRVHTEGDEYMVASESAALDILGFIRIRDVRPGEAVVITARGELFSEVC
ATPTNHTPCIFEYVYFARPDSMIDNISVHKARMRMGVKLGEKILRLRPNHDIDTIIPIPDTSRDAALEISNVLGVKYREG
FVKNRYVGRTFIMPGQGERVKSVRRKLNPIHLEFRNRVVLLIDDSIVRGTTSRQIVQMVRDAGARKVYLASAAPPVRYPN
VYGIDMPAADELIAHDRTEQEIQSLLGCDWLIYQDLEDLENAVREGNQEIKQFDSSCFNGHYITGIERGYLDRIRQLRSD
EVKRK

Sequences:

>Translated_485_residues
MCGILGIVGNQNVAGQLYDGLTVLQHRGQDAAGIATASGTRLRVQKANGLVRDVFDEQRMAVLDGCIGIAHCRYPTAGSE
GMDEAQPFYVNSPYGIALAHNGNLINTEALHQQVFEADRRNINTDSDSEVLLNVFAYELDAQRMLTPEAAIRAVAGVHRR
CKGGYAVVSMILGLGLVAFRDPHGIRPLVLGKRVHTEGDEYMVASESAALDILGFIRIRDVRPGEAVVITARGELFSEVC
ATPTNHTPCIFEYVYFARPDSMIDNISVHKARMRMGVKLGEKILRLRPNHDIDTIIPIPDTSRDAALEISNVLGVKYREG
FVKNRYVGRTFIMPGQGERVKSVRRKLNPIHLEFRNRVVLLIDDSIVRGTTSRQIVQMVRDAGARKVYLASAAPPVRYPN
VYGIDMPAADELIAHDRTEQEIQSLLGCDWLIYQDLEDLENAVREGNQEIKQFDSSCFNGHYITGIERGYLDRIRQLRSD
EVKRK
>Mature_485_residues
MCGILGIVGNQNVAGQLYDGLTVLQHRGQDAAGIATASGTRLRVQKANGLVRDVFDEQRMAVLDGCIGIAHCRYPTAGSE
GMDEAQPFYVNSPYGIALAHNGNLINTEALHQQVFEADRRNINTDSDSEVLLNVFAYELDAQRMLTPEAAIRAVAGVHRR
CKGGYAVVSMILGLGLVAFRDPHGIRPLVLGKRVHTEGDEYMVASESAALDILGFIRIRDVRPGEAVVITARGELFSEVC
ATPTNHTPCIFEYVYFARPDSMIDNISVHKARMRMGVKLGEKILRLRPNHDIDTIIPIPDTSRDAALEISNVLGVKYREG
FVKNRYVGRTFIMPGQGERVKSVRRKLNPIHLEFRNRVVLLIDDSIVRGTTSRQIVQMVRDAGARKVYLASAAPPVRYPN
VYGIDMPAADELIAHDRTEQEIQSLLGCDWLIYQDLEDLENAVREGNQEIKQFDSSCFNGHYITGIERGYLDRIRQLRSD
EVKRK

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=471, Percent_Identity=33.7579617834395, Blast_Score=253, Evalue=3e-67,
Organism=Escherichia coli, GI1788651, Length=488, Percent_Identity=61.6803278688525, Blast_Score=587, Evalue=1e-169,
Organism=Escherichia coli, GI1790167, Length=172, Percent_Identity=31.9767441860465, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17554892, Length=495, Percent_Identity=34.9494949494949, Blast_Score=240, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6323958, Length=491, Percent_Identity=54.5824847250509, Blast_Score=517, Evalue=1e-147,
Organism=Drosophila melanogaster, GI28573187, Length=495, Percent_Identity=33.7373737373737, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24659598, Length=499, Percent_Identity=32.064128256513, Blast_Score=238, Evalue=8e-63,
Organism=Drosophila melanogaster, GI24659604, Length=475, Percent_Identity=33.0526315789474, Blast_Score=237, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 54028; Mature: 54028

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGILGIVGNQNVAGQLYDGLTVLQHRGQDAAGIATASGTRLRVQKANGLVRDVFDEQRM
CCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCCHHHHHHHHHHH
AVLDGCIGIAHCRYPTAGSEGMDEAQPFYVNSPYGIALAHNGNLINTEALHQQVFEADRR
HHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCEECHHHHHHHHHHHHHC
NINTDSDSEVLLNVFAYELDAQRMLTPEAAIRAVAGVHRRCKGGYAVVSMILGLGLVAFR
CCCCCCCHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEC
DPHGIRPLVLGKRVHTEGDEYMVASESAALDILGFIRIRDVRPGEAVVITARGELFSEVC
CCCCCCCEEECCEECCCCCCEEEECCCCHHHEEEEEEEEECCCCCEEEEEECHHHHHHHH
ATPTNHTPCIFEYVYFARPDSMIDNISVHKARMRMGVKLGEKILRLRPNHDIDTIIPIPD
CCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCC
TSRDAALEISNVLGVKYREGFVKNRYVGRTFIMPGQGERVKSVRRKLNPIHLEFRNRVVL
CCCCHHHHHHHHHCCHHHHCHHHCCCCCEEEEECCCCHHHHHHHHHCCCEEEEECCEEEE
LIDDSIVRGTTSRQIVQMVRDAGARKVYLASAAPPVRYPNVYGIDMPAADELIAHDRTEQ
EECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHCCCCHH
EIQSLLGCDWLIYQDLEDLENAVREGNQEIKQFDSSCFNGHYITGIERGYLDRIRQLRSD
HHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHH
EVKRK
HHCCC
>Mature Secondary Structure
MCGILGIVGNQNVAGQLYDGLTVLQHRGQDAAGIATASGTRLRVQKANGLVRDVFDEQRM
CCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCCHHHHHHHHHHH
AVLDGCIGIAHCRYPTAGSEGMDEAQPFYVNSPYGIALAHNGNLINTEALHQQVFEADRR
HHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCEECHHHHHHHHHHHHHC
NINTDSDSEVLLNVFAYELDAQRMLTPEAAIRAVAGVHRRCKGGYAVVSMILGLGLVAFR
CCCCCCCHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEC
DPHGIRPLVLGKRVHTEGDEYMVASESAALDILGFIRIRDVRPGEAVVITARGELFSEVC
CCCCCCCEEECCEECCCCCCEEEECCCCHHHEEEEEEEEECCCCCEEEEEECHHHHHHHH
ATPTNHTPCIFEYVYFARPDSMIDNISVHKARMRMGVKLGEKILRLRPNHDIDTIIPIPD
CCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCC
TSRDAALEISNVLGVKYREGFVKNRYVGRTFIMPGQGERVKSVRRKLNPIHLEFRNRVVL
CCCCHHHHHHHHHCCHHHHCHHHCCCCCEEEEECCCCHHHHHHHHHCCCEEEEECCEEEE
LIDDSIVRGTTSRQIVQMVRDAGARKVYLASAAPPVRYPNVYGIDMPAADELIAHDRTEQ
EECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHCCCCHH
EIQSLLGCDWLIYQDLEDLENAVREGNQEIKQFDSSCFNGHYITGIERGYLDRIRQLRSD
HHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHH
EVKRK
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]