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The map label for this gene is gshB [H]

Identifier: 182681441

GI number: 182681441

Start: 1056278

End: 1057222

Strand: Reverse

Name: gshB [H]

Synonym: XfasM23_0892

Alternate gene names: 182681441

Gene position: 1057222-1056278 (Counterclockwise)

Preceding gene: 182681448

Following gene: 182681440

Centisome position: 41.69

GC content: 53.76

Gene sequence:

>945_bases
ATGCCGTTGGACGTCGTTGTTGTGATGGACCCCATCACAGGTATCAAAATCGCCAAAGACACCACCTTTGCATTGCTGCT
GGAGGCGCAGCGTCGCAGCCATCGCCTTCATTATGTGCACCCTGGTAGCCTCAGCTTAAATGAGGGGCGTACCTTCGCGC
AAACCGCACCGCTTCAAGTACGCGACAACAAGACCGACTGGTACACCTTGGGGGAGTTCAGCGAGACTCAACTCGGCCAA
GGGCAAATAATCCTGATGCGTAAAGATCCTCCAGTCAATGCTGAATTCATCTACGACACACAGTTGCTTAGTATCGCCCA
AGCCGCTGGTGCTCAAGTGATTAACCACCCACAGGGCCTACGCGATCTCAACGAGAAAATAGCCGCGCAGCTATTCCCAC
AATGTTGCCCACCAACTCTTATTAGCCGTGATATGAGAGCACTGAAGATGTTTGTTCAGAAGCAAGAGCAAGCGATTCTC
AAGCCACTAGACGGGATGGGTGGGCATTCGATATTTCGCAGTAGCAACGGCGACCCCAATCTCAATGTGATCCTGGAAAC
GTTGACAGATGGCGGACGCACACTGGCAATAGCGCAACGCTATCTGCAACAAATTATCGAAGGCGACAAACGCATCCTAC
TGATTGACGGAGTCCCTATAGACCATTGTTTGGCTAGAATCCCACAAGGTGACGAATTCCGCGGCAACATGGCTGCTGGA
GGCCGCGGTGAGAGCCGCCCCCTCAACGAACGCGACCGCTGGATCGCCGCTCAAGTCGGGCCAGAGATGCGACGCCGTGG
CATGCGTTTTGTCGGAATCGACGTGATCGGCGACTACTTGACTGAGATAAACGTCACAAGCCCCACCTGCCTACGCGAAT
TAGACGCACAGTGCGGCCTGAACATCGCTGGACAACTGTTCGATGCAATTGAAACTGGAGGCTGA

Upstream 100 bases:

>100_bases
AGCATCGCATGCAAGCACCATATAGGGCAAAACAGGCACACTATTAGCTCCCTAAACTCGCTAACATCGGCATCTCTGTT
TCAAATCAGGTTGACCTTTA

Downstream 100 bases:

>100_bases
TATGGATGCGGTCAATACAGCCCAAATCCGGGAACGTCAACGTCTGAACACAACACTGGTTCTGTCGATTCTCATACACC
TTGCGCTGATTCTAGGAGTA

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MPLDVVVVMDPITGIKIAKDTTFALLLEAQRRSHRLHYVHPGSLSLNEGRTFAQTAPLQVRDNKTDWYTLGEFSETQLGQ
GQIILMRKDPPVNAEFIYDTQLLSIAQAAGAQVINHPQGLRDLNEKIAAQLFPQCCPPTLISRDMRALKMFVQKQEQAIL
KPLDGMGGHSIFRSSNGDPNLNVILETLTDGGRTLAIAQRYLQQIIEGDKRILLIDGVPIDHCLARIPQGDEFRGNMAAG
GRGESRPLNERDRWIAAQVGPEMRRRGMRFVGIDVIGDYLTEINVTSPTCLRELDAQCGLNIAGQLFDAIETGG

Sequences:

>Translated_314_residues
MPLDVVVVMDPITGIKIAKDTTFALLLEAQRRSHRLHYVHPGSLSLNEGRTFAQTAPLQVRDNKTDWYTLGEFSETQLGQ
GQIILMRKDPPVNAEFIYDTQLLSIAQAAGAQVINHPQGLRDLNEKIAAQLFPQCCPPTLISRDMRALKMFVQKQEQAIL
KPLDGMGGHSIFRSSNGDPNLNVILETLTDGGRTLAIAQRYLQQIIEGDKRILLIDGVPIDHCLARIPQGDEFRGNMAAG
GRGESRPLNERDRWIAAQVGPEMRRRGMRFVGIDVIGDYLTEINVTSPTCLRELDAQCGLNIAGQLFDAIETGG
>Mature_313_residues
PLDVVVVMDPITGIKIAKDTTFALLLEAQRRSHRLHYVHPGSLSLNEGRTFAQTAPLQVRDNKTDWYTLGEFSETQLGQG
QIILMRKDPPVNAEFIYDTQLLSIAQAAGAQVINHPQGLRDLNEKIAAQLFPQCCPPTLISRDMRALKMFVQKQEQAILK
PLDGMGGHSIFRSSNGDPNLNVILETLTDGGRTLAIAQRYLQQIIEGDKRILLIDGVPIDHCLARIPQGDEFRGNMAAGG
RGESRPLNERDRWIAAQVGPEMRRRGMRFVGIDVIGDYLTEINVTSPTCLRELDAQCGLNIAGQLFDAIETGG

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=305, Percent_Identity=53.4426229508197, Blast_Score=334, Evalue=5e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 34735; Mature: 34604

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLDVVVVMDPITGIKIAKDTTFALLLEAQRRSHRLHYVHPGSLSLNEGRTFAQTAPLQV
CCEEEEEEECCCCCEEEECCCHHHHHHHHHHHCCCEEEECCCCEECCCCCEEEECCCEEE
RDNKTDWYTLGEFSETQLGQGQIILMRKDPPVNAEFIYDTQLLSIAQAAGAQVINHPQGL
ECCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHCHHHHCCCHHH
RDLNEKIAAQLFPQCCPPTLISRDMRALKMFVQKQEQAILKPLDGMGGHSIFRSSNGDPN
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCC
LNVILETLTDGGRTLAIAQRYLQQIIEGDKRILLIDGVPIDHCLARIPQGDEFRGNMAAG
CEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCC
GRGESRPLNERDRWIAAQVGPEMRRRGMRFVGIDVIGDYLTEINVTSPTCLRELDAQCGL
CCCCCCCCCHHHCEEEECCCHHHHHCCCEEEEHHHHHHHHHHCCCCCHHHHHHHHHHCCC
NIAGQLFDAIETGG
CHHHHHHHHHHCCC
>Mature Secondary Structure 
PLDVVVVMDPITGIKIAKDTTFALLLEAQRRSHRLHYVHPGSLSLNEGRTFAQTAPLQV
CEEEEEEECCCCCEEEECCCHHHHHHHHHHHCCCEEEECCCCEECCCCCEEEECCCEEE
RDNKTDWYTLGEFSETQLGQGQIILMRKDPPVNAEFIYDTQLLSIAQAAGAQVINHPQGL
ECCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHCHHHHCCCHHH
RDLNEKIAAQLFPQCCPPTLISRDMRALKMFVQKQEQAILKPLDGMGGHSIFRSSNGDPN
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCC
LNVILETLTDGGRTLAIAQRYLQQIIEGDKRILLIDGVPIDHCLARIPQGDEFRGNMAAG
CEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCC
GRGESRPLNERDRWIAAQVGPEMRRRGMRFVGIDVIGDYLTEINVTSPTCLRELDAQCGL
CCCCCCCCCHHHCEEEECCCHHHHHCCCEEEEHHHHHHHHHHCCCCCHHHHHHHHHHCCC
NIAGQLFDAIETGG
CHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]