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The map label for this gene is purB [H]

Identifier: 182681358

GI number: 182681358

Start: 952440

End: 953807

Strand: Reverse

Name: purB [H]

Synonym: XfasM23_0805

Alternate gene names: 182681358

Gene position: 953807-952440 (Counterclockwise)

Preceding gene: 182681361

Following gene: 182681357

Centisome position: 37.62

GC content: 53.14

Gene sequence:

>1368_bases
ATGTCACATTTACCCCTACTTGCTTTGTCGCCACTGGATGGGCGCTATTTGTCTAAAGTCGATACATTGCGGCCAATTTT
TTCAGAATACGGCTTGATCAAATCCAGGGTGAAAGTCGAGATTGCATGGTTACTGGCGTTGGCCGCAGAACCAAACATTG
TCGAGTTGGCGCCGTTCTCCGTTGCCGCGCAAACACGTCTGCGTGTGTTAGCAGACGGTTTGACCATTGAGCACGCCGCA
CGTGTGAAAGAGATTGAGCACACCACCAATCACGACGTAAAAGCAGTGGAATATTTCATCAAAGAGCAGCTTAAGGACGA
TATAGAGCTGCGCCGCGGACTGGAGTTTGTCCATTTCGCTTGCACCAGCGAGGACATCAACAACCTGTCCTATAGCTTGA
TGTTGCAACAAGCCAGAGATGACGTACTGCTCCCGACACTGGACGGTATCGCTACACATCTGCGCACCTTGGCCCATGCT
CAGGCCGCACAACCGATGCTCTCACGCACCCACGGGCAAACCGCCTCCCCTACCACGCTGGGCAAGGAAATCGCCAATGT
GGTAGCCCGTTTGGAACGCCAGCGCCATCAGATCGCCACGATTGCACTGAGCGGAAAGATCAACGGTGCTGTAGGCAATT
ACAACGCACATACCATCAGCTATCCGGAGGTCAATTGGCCTGCATTGGCACAACGTTTTGTTGAGAGCCTGGGCTTGGTC
TTCAACCCATACACCACACAAATTGAGCCGCACGACACCATCGCCGAGATCAGCGACGCAATCCGCCGCGCCAACACCAT
TCTGATCGACTTGGCACGCGATCTTTGGGGCTATATCTCGTTGGGTTACTTCAAACAAAAGCTCAAGGAAGGTGAAATCG
GCTCCTCAACCATGCCGCATAAAGTCAATCCGATTGACTTCGAAAATGCTGAAGGCAATTTCGGCGTGGCCAACGCATTA
TTTGAGCATTTTTCGACCAAGCTGCCGATCAGTCGCTGGCAACGTGACCTCACCGATTCCACCGTACTACGCGCACTCGG
CACCGCGTTTGGCCATACTCAGGTGGCTTTAGATTCTTTAAACAAAGGGCTACACAAGCTCAGCCCAAATCCAGAACGCA
TTGACGCTGACCTGGACGCGGCATGGGAAGTGCTGGCCGAAGCAGTACAGACAATCATGCGCCGCCACAATCTACCCAAC
CCCTACGAGCAACTGAAAGCGCTGACCCGTGGTCAGGGCATCACCGCCGATTCGATGCGCGCTTTCATCGAAACCCTGGA
TCTACCAACTGAAGCCAAACAGCGGCTGCACGATTTAACTCCAAGGGCTTATACCGGTCTGGCGAAGCAGTTGGCACTGA
ACATCTAA

Upstream 100 bases:

>100_bases
CACAGGATCACAGGATCAGGAATGGACCATATTCCAACCCAGAAAGGGCAGACAGGTGCAGCGTAGAATAGTGGCGCTCC
CGCTTCCCTCAATTACGGCC

Downstream 100 bases:

>100_bases
GTGAGTCGAAGAAGTGTGATGTAGGGCCATACATTGCAATGAATTTCCCAGAATATTCCGTCCGCACATCACAAATATCC
CCATCAATTGATCATTCAAA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MSHLPLLALSPLDGRYLSKVDTLRPIFSEYGLIKSRVKVEIAWLLALAAEPNIVELAPFSVAAQTRLRVLADGLTIEHAA
RVKEIEHTTNHDVKAVEYFIKEQLKDDIELRRGLEFVHFACTSEDINNLSYSLMLQQARDDVLLPTLDGIATHLRTLAHA
QAAQPMLSRTHGQTASPTTLGKEIANVVARLERQRHQIATIALSGKINGAVGNYNAHTISYPEVNWPALAQRFVESLGLV
FNPYTTQIEPHDTIAEISDAIRRANTILIDLARDLWGYISLGYFKQKLKEGEIGSSTMPHKVNPIDFENAEGNFGVANAL
FEHFSTKLPISRWQRDLTDSTVLRALGTAFGHTQVALDSLNKGLHKLSPNPERIDADLDAAWEVLAEAVQTIMRRHNLPN
PYEQLKALTRGQGITADSMRAFIETLDLPTEAKQRLHDLTPRAYTGLAKQLALNI

Sequences:

>Translated_455_residues
MSHLPLLALSPLDGRYLSKVDTLRPIFSEYGLIKSRVKVEIAWLLALAAEPNIVELAPFSVAAQTRLRVLADGLTIEHAA
RVKEIEHTTNHDVKAVEYFIKEQLKDDIELRRGLEFVHFACTSEDINNLSYSLMLQQARDDVLLPTLDGIATHLRTLAHA
QAAQPMLSRTHGQTASPTTLGKEIANVVARLERQRHQIATIALSGKINGAVGNYNAHTISYPEVNWPALAQRFVESLGLV
FNPYTTQIEPHDTIAEISDAIRRANTILIDLARDLWGYISLGYFKQKLKEGEIGSSTMPHKVNPIDFENAEGNFGVANAL
FEHFSTKLPISRWQRDLTDSTVLRALGTAFGHTQVALDSLNKGLHKLSPNPERIDADLDAAWEVLAEAVQTIMRRHNLPN
PYEQLKALTRGQGITADSMRAFIETLDLPTEAKQRLHDLTPRAYTGLAKQLALNI
>Mature_454_residues
SHLPLLALSPLDGRYLSKVDTLRPIFSEYGLIKSRVKVEIAWLLALAAEPNIVELAPFSVAAQTRLRVLADGLTIEHAAR
VKEIEHTTNHDVKAVEYFIKEQLKDDIELRRGLEFVHFACTSEDINNLSYSLMLQQARDDVLLPTLDGIATHLRTLAHAQ
AAQPMLSRTHGQTASPTTLGKEIANVVARLERQRHQIATIALSGKINGAVGNYNAHTISYPEVNWPALAQRFVESLGLVF
NPYTTQIEPHDTIAEISDAIRRANTILIDLARDLWGYISLGYFKQKLKEGEIGSSTMPHKVNPIDFENAEGNFGVANALF
EHFSTKLPISRWQRDLTDSTVLRALGTAFGHTQVALDSLNKGLHKLSPNPERIDADLDAAWEVLAEAVQTIMRRHNLPNP
YEQLKALTRGQGITADSMRAFIETLDLPTEAKQRLHDLTPRAYTGLAKQLALNI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=443, Percent_Identity=56.8848758465011, Blast_Score=518, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50640; Mature: 50509

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHLPLLALSPLDGRYLSKVDTLRPIFSEYGLIKSRVKVEIAWLLALAAEPNIVELAPFS
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHH
VAAQTRLRVLADGLTIEHAARVKEIEHTTNHDVKAVEYFIKEQLKDDIELRRGLEFVHFA
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEEE
CTSEDINNLSYSLMLQQARDDVLLPTLDGIATHLRTLAHAQAAQPMLSRTHGQTASPTTL
ECHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
GKEIANVVARLERQRHQIATIALSGKINGAVGNYNAHTISYPEVNWPALAQRFVESLGLV
HHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCE
FNPYTTQIEPHDTIAEISDAIRRANTILIDLARDLWGYISLGYFKQKLKEGEIGSSTMPH
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
KVNPIDFENAEGNFGVANALFEHFSTKLPISRWQRDLTDSTVLRALGTAFGHTQVALDSL
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
NKGLHKLSPNPERIDADLDAAWEVLAEAVQTIMRRHNLPNPYEQLKALTRGQGITADSMR
HHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
AFIETLDLPTEAKQRLHDLTPRAYTGLAKQLALNI
HHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SHLPLLALSPLDGRYLSKVDTLRPIFSEYGLIKSRVKVEIAWLLALAAEPNIVELAPFS
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHH
VAAQTRLRVLADGLTIEHAARVKEIEHTTNHDVKAVEYFIKEQLKDDIELRRGLEFVHFA
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEEE
CTSEDINNLSYSLMLQQARDDVLLPTLDGIATHLRTLAHAQAAQPMLSRTHGQTASPTTL
ECHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
GKEIANVVARLERQRHQIATIALSGKINGAVGNYNAHTISYPEVNWPALAQRFVESLGLV
HHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCE
FNPYTTQIEPHDTIAEISDAIRRANTILIDLARDLWGYISLGYFKQKLKEGEIGSSTMPH
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
KVNPIDFENAEGNFGVANALFEHFSTKLPISRWQRDLTDSTVLRALGTAFGHTQVALDSL
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
NKGLHKLSPNPERIDADLDAAWEVLAEAVQTIMRRHNLPNPYEQLKALTRGQGITADSMR
HHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
AFIETLDLPTEAKQRLHDLTPRAYTGLAKQLALNI
HHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]