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The map label for this gene is hemK [H]

Identifier: 182681318

GI number: 182681318

Start: 902043

End: 902870

Strand: Reverse

Name: hemK [H]

Synonym: XfasM23_0765

Alternate gene names: 182681318

Gene position: 902870-902043 (Counterclockwise)

Preceding gene: 182681341

Following gene: 182681316

Centisome position: 35.61

GC content: 58.57

Gene sequence:

>828_bases
ATGCCATCACCGATCGCTTTGCTTGCCGCCGCCACTGAACGCATTGAGCGAGTCGATGCAGAAGCCTTGCTGTTGCATGC
TCTGGACTGCGATCGGGCGTGGCTGTTCACCCATGGGGACATCCCCCTGGCAGCGGCAGCGACGGAGAGCTTCCAAGCGC
TCGTGGAGCAACGTGCTCGCGGTATCCCCGTGGCTTATCTGATTGGGCGGCGTGGATTTTGGACGCTTGATGTAATTGTG
TCCTCAGCAACGCTGATTCCGCGTGCAGAAACAGAAACGTTGGTCGAACAAGCATTGCAGCGACTCGACCATGCTTCAGA
GCGACGCGTGGCCGATCTGGGCACCGGCAGCGGCGCCATTGCACTGGCTATTGCTTGCGAACGGCCGCAAGCACAGGTGT
TAGCCACCGATAACAGCGCAGCCGCGCTCGACATTGCCGCACGCAATGCCAGCGCACATGGGCTAAATCATGTGGTGTTT
CGAGAAGGCAATTGGTATGAGGCACTGTTGGGGGAACGCTTTGATCTGATCGTCAGCAATCCCCCTTACATTGCCGTCAC
TGATCCACATTTGACGCAGGGTGATCTGCGTTTTGAACCACCATCGGCATTGATCTCTGGTGGCGACGGATTGGACGCAC
TGCGCATCCTCGCTGCAGGGGCACCAGCACATTTACGGCCAGGGGGTTGGTTAGTGCTGGAGCATGGTTGGGATCAAGGT
GCGGCCGTGCGAACATTACTGCACACTGCGGGATTGGTGGCAGTGGCCACCATGCAAGACTTGGAAGCACGCGACCGCGT
CACCGTGGGGCGCTGTCCTGGTTTTTAA

Upstream 100 bases:

>100_bases
CAGCAATCAGTGTTGCCGTCTTCATTTTCTTAAAGGATGCAGTGATAGGGATGAGGACCGATAATCAGCACCCTGCTTTG
CTCCACAATCCAGCCTTTCA

Downstream 100 bases:

>100_bases
AAGCACAGCGGTACTGTGTGTCGTACTCAATACAATGCAGGCCATTTTCAAGTCGGCATCCCAGCCAAAGGCTGCTCATC
TGTGGAGCGCATTGCGGTCG

Product: protein-(glutamine-N5) methyltransferase, release factor-specific

Products: NA

Alternate protein names: M.XfaHemK2P [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MPSPIALLAAATERIERVDAEALLLHALDCDRAWLFTHGDIPLAAAATESFQALVEQRARGIPVAYLIGRRGFWTLDVIV
SSATLIPRAETETLVEQALQRLDHASERRVADLGTGSGAIALAIACERPQAQVLATDNSAAALDIAARNASAHGLNHVVF
REGNWYEALLGERFDLIVSNPPYIAVTDPHLTQGDLRFEPPSALISGGDGLDALRILAAGAPAHLRPGGWLVLEHGWDQG
AAVRTLLHTAGLVAVATMQDLEARDRVTVGRCPGF

Sequences:

>Translated_275_residues
MPSPIALLAAATERIERVDAEALLLHALDCDRAWLFTHGDIPLAAAATESFQALVEQRARGIPVAYLIGRRGFWTLDVIV
SSATLIPRAETETLVEQALQRLDHASERRVADLGTGSGAIALAIACERPQAQVLATDNSAAALDIAARNASAHGLNHVVF
REGNWYEALLGERFDLIVSNPPYIAVTDPHLTQGDLRFEPPSALISGGDGLDALRILAAGAPAHLRPGGWLVLEHGWDQG
AAVRTLLHTAGLVAVATMQDLEARDRVTVGRCPGF
>Mature_274_residues
PSPIALLAAATERIERVDAEALLLHALDCDRAWLFTHGDIPLAAAATESFQALVEQRARGIPVAYLIGRRGFWTLDVIVS
SATLIPRAETETLVEQALQRLDHASERRVADLGTGSGAIALAIACERPQAQVLATDNSAAALDIAARNASAHGLNHVVFR
EGNWYEALLGERFDLIVSNPPYIAVTDPHLTQGDLRFEPPSALISGGDGLDALRILAAGAPAHLRPGGWLVLEHGWDQGA
AVRTLLHTAGLVAVATMQDLEARDRVTVGRCPGF

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=242, Percent_Identity=30.1652892561983, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1787463, Length=256, Percent_Identity=45.703125, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI87082085, Length=176, Percent_Identity=35.7954545454545, Blast_Score=98, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24582226, Length=227, Percent_Identity=32.15859030837, Blast_Score=111, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 29330; Mature: 29198

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPIALLAAATERIERVDAEALLLHALDCDRAWLFTHGDIPLAAAATESFQALVEQRAR
CCCCHHHHHHHHHHHHHHCHHHHEEEHCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHC
GIPVAYLIGRRGFWTLDVIVSSATLIPRAETETLVEQALQRLDHASERRVADLGTGSGAI
CCCEEEEECCCCCEEEEEECCCCEECCCCHHHHHHHHHHHHHCCHHHCCCHHCCCCCCEE
ALAIACERPQAQVLATDNSAAALDIAARNASAHGLNHVVFREGNWYEALLGERFDLIVSN
EEEEEECCCCCEEEEECCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHHCCCEEEEECC
PPYIAVTDPHLTQGDLRFEPPSALISGGDGLDALRILAAGAPAHLRPGGWLVLEHGWDQG
CCEEEEECCCCCCCCEEECCCHHEEECCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCC
AAVRTLLHTAGLVAVATMQDLEARDRVTVGRCPGF
HHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCC
>Mature Secondary Structure 
PSPIALLAAATERIERVDAEALLLHALDCDRAWLFTHGDIPLAAAATESFQALVEQRAR
CCCHHHHHHHHHHHHHHCHHHHEEEHCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHC
GIPVAYLIGRRGFWTLDVIVSSATLIPRAETETLVEQALQRLDHASERRVADLGTGSGAI
CCCEEEEECCCCCEEEEEECCCCEECCCCHHHHHHHHHHHHHCCHHHCCCHHCCCCCCEE
ALAIACERPQAQVLATDNSAAALDIAARNASAHGLNHVVFREGNWYEALLGERFDLIVSN
EEEEEECCCCCEEEEECCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHHCCCEEEEECC
PPYIAVTDPHLTQGDLRFEPPSALISGGDGLDALRILAAGAPAHLRPGGWLVLEHGWDQG
CCEEEEECCCCCCCCEEECCCHHEEECCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCC
AAVRTLLHTAGLVAVATMQDLEARDRVTVGRCPGF
HHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]