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The map label for this gene is 182681242

Identifier: 182681242

GI number: 182681242

Start: 813119

End: 814483

Strand: Reverse

Name: 182681242

Synonym: XfasM23_0687

Alternate gene names: NA

Gene position: 814483-813119 (Counterclockwise)

Preceding gene: 182681243

Following gene: 182681240

Centisome position: 32.12

GC content: 55.75

Gene sequence:

>1365_bases
TTGTCGAGTCCGAGTCACATTACCGAGATCCCAATCACCAACCGCGCGCAACTCGTAGAAGTCCTGGCATCTGGCGAAAA
ACCCGCCTCCCAGTGGCGCATTGGCACCGAACACGAGAAATTCGGCTTCCGTTTGGACGACCTGCGCCCACCAACATTCG
ATGATGAACGCGGTATTGAGGCATTGTTGACCGGCATGACCCGCTTTGGCTGGACACCGGTACAAGAACATGACCGTACC
ATCGCACTGCTACGCGGCGATACCTCCATCACCCTCGAACCGGCCGGTCAACTTGAACTCGCCGGCGCCCCCTTAGAAAC
CATCCATCAAACCCACATCGAAGCTGAAACACACCTACGCGAAGTCAACGAGATTGCCAACACCCTGCAACTCGGATTCA
TAGGCATGGGTTTCCACCCCAAGTGCACACGTGCGCAAATGCCGTGGATGCCCAAAGGCCGCTACGCAATCATGCGTGCC
TACATGCCCAAAGTTGGCCAACTCGGCCTTGACATGATGACCCGTACTTGCACCGTACAAGTCAACCTCGACTACGCCAG
CGAAACAGATATGGTGAAAAAATTCCGGGTTTCACTAGCACTGCAACCGATCGCCACAGCATTGTTTGCAAACTCCCCAT
TCACCGAAGGCAAACCCAACGGTTACTTGAGCTACCGCTCACACATTTGGACCGACACCGACCCAGACCGCACCGGCATG
CTTGATTTCGTTTTTGAGGATGGCTTCGGCTATGAACGCTACGTCGACTACTTACTTGACGTCCCGATGTACTTCTCTTA
CCGCACTGGCATCTACCACGACGCCAGCGGGCAGAGCTTTCGCGACTTCCTGCAAGGACGCCTACCCGCCCTTCCTGGCA
TCTTACCAACCCTCCGTGATTGGTCCGATCACATGACAACCGCATTCCCTGAAGTACGTCTAAAGAAATACATAGAGATG
CGCGGCGCCGACTCCGGCCCACTGCCAACCCTGTGCGCACTCCCCGCCTTCTGGGTGGGCCTGCTGTACGACAACACCGC
ACTGGATGCCGCCTGGGACCTGATTAAAGATTTGACTCTGCAAGAACGCCACGCCCTGCGCAACGGCGTGCCCCGACATG
CCTTGGCACTACCATTCCGCAACAGCACCGTGCGCACACTGGCACTTCAAACCCTGGACATCTCACGCGCCGGCTTACGT
CGCCGCGCACAGCACAACGCCAACGGTCAAGACGAAACCATCTTTCTCAATGTCCTTGATGAGATCGCTCACAGCGGCCA
AACACCTGCACAACGCAAACTACAACTGTATGAAAGCACCTGGAATCACGACATCAATCCGATCTTCAATGACTACGCCT
ATTGA

Upstream 100 bases:

>100_bases
TCAATCCTTTCAGAGACATACTGCGGTTATAGTGCCGCACTGCGATGCTTAAGGAACCATCCTCGCATCGCCCCATCTAC
ATTCACTGCGAGCCCTACCC

Downstream 100 bases:

>100_bases
AGCCCGTAAAGACGCTCCCCCTGCCCTAATATCCCCCTTTTCCTACACACCCCACCGATTTCCACTCAGCCACATTGACC
CCAACCAACATTAGATTTCC

Product: glutamate--cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Gamma-Glutamylcysteine Synthetase; Glutamate/Cysteine Ligase Family Protein; Glutamate-Cysteine Ligase GshA; Glutamylcysteine Synthetase; Glutamate-Cysteine Ligase Family 2 Protein; Glutamate-Cysteine Ligase Protein; Glutamate-Cysteine Ligase Protein; Glutamate-Cysteine Ligase Family Protein; Glutathione Synthetase; Glutamate Cystein Ligase Protein; Glutamate-Cysteine Ligase Gsha; Glutamate-Cysteine Ligase GSHA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSSPSHITEIPITNRAQLVEVLASGEKPASQWRIGTEHEKFGFRLDDLRPPTFDDERGIEALLTGMTRFGWTPVQEHDRT
IALLRGDTSITLEPAGQLELAGAPLETIHQTHIEAETHLREVNEIANTLQLGFIGMGFHPKCTRAQMPWMPKGRYAIMRA
YMPKVGQLGLDMMTRTCTVQVNLDYASETDMVKKFRVSLALQPIATALFANSPFTEGKPNGYLSYRSHIWTDTDPDRTGM
LDFVFEDGFGYERYVDYLLDVPMYFSYRTGIYHDASGQSFRDFLQGRLPALPGILPTLRDWSDHMTTAFPEVRLKKYIEM
RGADSGPLPTLCALPAFWVGLLYDNTALDAAWDLIKDLTLQERHALRNGVPRHALALPFRNSTVRTLALQTLDISRAGLR
RRAQHNANGQDETIFLNVLDEIAHSGQTPAQRKLQLYESTWNHDINPIFNDYAY

Sequences:

>Translated_454_residues
MSSPSHITEIPITNRAQLVEVLASGEKPASQWRIGTEHEKFGFRLDDLRPPTFDDERGIEALLTGMTRFGWTPVQEHDRT
IALLRGDTSITLEPAGQLELAGAPLETIHQTHIEAETHLREVNEIANTLQLGFIGMGFHPKCTRAQMPWMPKGRYAIMRA
YMPKVGQLGLDMMTRTCTVQVNLDYASETDMVKKFRVSLALQPIATALFANSPFTEGKPNGYLSYRSHIWTDTDPDRTGM
LDFVFEDGFGYERYVDYLLDVPMYFSYRTGIYHDASGQSFRDFLQGRLPALPGILPTLRDWSDHMTTAFPEVRLKKYIEM
RGADSGPLPTLCALPAFWVGLLYDNTALDAAWDLIKDLTLQERHALRNGVPRHALALPFRNSTVRTLALQTLDISRAGLR
RRAQHNANGQDETIFLNVLDEIAHSGQTPAQRKLQLYESTWNHDINPIFNDYAY
>Mature_453_residues
SSPSHITEIPITNRAQLVEVLASGEKPASQWRIGTEHEKFGFRLDDLRPPTFDDERGIEALLTGMTRFGWTPVQEHDRTI
ALLRGDTSITLEPAGQLELAGAPLETIHQTHIEAETHLREVNEIANTLQLGFIGMGFHPKCTRAQMPWMPKGRYAIMRAY
MPKVGQLGLDMMTRTCTVQVNLDYASETDMVKKFRVSLALQPIATALFANSPFTEGKPNGYLSYRSHIWTDTDPDRTGML
DFVFEDGFGYERYVDYLLDVPMYFSYRTGIYHDASGQSFRDFLQGRLPALPGILPTLRDWSDHMTTAFPEVRLKKYIEMR
GADSGPLPTLCALPAFWVGLLYDNTALDAAWDLIKDLTLQERHALRNGVPRHALALPFRNSTVRTLALQTLDISRAGLRR
RAQHNANGQDETIFLNVLDEIAHSGQTPAQRKLQLYESTWNHDINPIFNDYAY

Specific function: Unknown

COG id: COG3572

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 51444; Mature: 51313

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPSHITEIPITNRAQLVEVLASGEKPASQWRIGTEHEKFGFRLDDLRPPTFDDERGIE
CCCCCCEEECCCCCHHHHHHHHHCCCCCHHHCCCCCCHHHCCCEECCCCCCCCCCCCCHH
ALLTGMTRFGWTPVQEHDRTIALLRGDTSITLEPAGQLELAGAPLETIHQTHIEAETHLR
HHHHHHHHHCCCCCCCCCCEEEEEECCCEEEECCCCCEEECCCCHHHHHHHHHHHHHHHH
EVNEIANTLQLGFIGMGFHPKCTRAQMPWMPKGRYAIMRAYMPKVGQLGLDMMTRTCTVQ
HHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHCEEEEEE
VNLDYASETDMVKKFRVSLALQPIATALFANSPFTEGKPNGYLSYRSHIWTDTDPDRTGM
EEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCC
LDFVFEDGFGYERYVDYLLDVPMYFSYRTGIYHDASGQSFRDFLQGRLPALPGILPTLRD
EEEEEECCCCHHHHHHHHHCCCHHEEECCCCEECCCCHHHHHHHHCCCCCCCCHHHHHHH
WSDHMTTAFPEVRLKKYIEMRGADSGPLPTLCALPAFWVGLLYDNTALDAAWDLIKDLTL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
QERHALRNGVPRHALALPFRNSTVRTLALQTLDISRAGLRRRAQHNANGQDETIFLNVLD
HHHHHHHCCCCCCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHH
EIAHSGQTPAQRKLQLYESTWNHDINPIFNDYAY
HHHHCCCCCHHHHHHHHHHHCCCCCCCCHHCCCC
>Mature Secondary Structure 
SSPSHITEIPITNRAQLVEVLASGEKPASQWRIGTEHEKFGFRLDDLRPPTFDDERGIE
CCCCCEEECCCCCHHHHHHHHHCCCCCHHHCCCCCCHHHCCCEECCCCCCCCCCCCCHH
ALLTGMTRFGWTPVQEHDRTIALLRGDTSITLEPAGQLELAGAPLETIHQTHIEAETHLR
HHHHHHHHHCCCCCCCCCCEEEEEECCCEEEECCCCCEEECCCCHHHHHHHHHHHHHHHH
EVNEIANTLQLGFIGMGFHPKCTRAQMPWMPKGRYAIMRAYMPKVGQLGLDMMTRTCTVQ
HHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHCEEEEEE
VNLDYASETDMVKKFRVSLALQPIATALFANSPFTEGKPNGYLSYRSHIWTDTDPDRTGM
EEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCC
LDFVFEDGFGYERYVDYLLDVPMYFSYRTGIYHDASGQSFRDFLQGRLPALPGILPTLRD
EEEEEECCCCHHHHHHHHHCCCHHEEECCCCEECCCCHHHHHHHHCCCCCCCCHHHHHHH
WSDHMTTAFPEVRLKKYIEMRGADSGPLPTLCALPAFWVGLLYDNTALDAAWDLIKDLTL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
QERHALRNGVPRHALALPFRNSTVRTLALQTLDISRAGLRRRAQHNANGQDETIFLNVLD
HHHHHHHCCCCCCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHH
EIAHSGQTPAQRKLQLYESTWNHDINPIFNDYAY
HHHHCCCCCHHHHHHHHHHHCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA