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The map label for this gene is gloA [H]

Identifier: 182681218

GI number: 182681218

Start: 787625

End: 788152

Strand: Reverse

Name: gloA [H]

Synonym: XfasM23_0662

Alternate gene names: 182681218

Gene position: 788152-787625 (Counterclockwise)

Preceding gene: 182681219

Following gene: 182681213

Centisome position: 31.08

GC content: 53.03

Gene sequence:

>528_bases
ATGCACCCCAAAAACTTCCAACATCAGCTCAAAACCATCCCACAACCTCCGGAAGAAACCCGGGACTTCGTGTTCAATCA
CACCATGCTACGGGTCAAAGACATCAACGCTTCTCTGGATTTCTACGCTCGCATACTCGGCTTCCGCCTGATCGACCAAC
GGGACTTCCCCGAAGCACAATTCAGCCTGTACTTCCTCGCACTGTTACCCCAGACGGTACACATCTCAGACAATGACACC
GAACGCCGCCTGTGGATGTCCGGCATCCCCGGAGTCCTTGAATTGACCCACAACTACGGAACCGAAACCCAAGAAGGACA
GATCTACCACAACGGCAACAGCGAACCGCGCGGCTTTGGCCACATCTGCATCTCCGTGCCCGATCTGTACAGCGCCTGCG
CACGTTTTGACACACTCCAAGTGCCTTATCAAAAACGCCTAACCGATGGCCGTATGAAAAACATCGCCTTCATCAAAGAT
CCAGACGGCTATTGGGTAGAAATCATCTCCAACACACCGCTGCCATAA

Upstream 100 bases:

>100_bases
ATCGACAACCTCAAAAGAAGCCCCCCAAAGGGGTAGCAATACTCCATCCTTGCACATCAACCCGTAAACACACCGGCCTT
TCCTACGCAAAATATCTCCT

Downstream 100 bases:

>100_bases
TCGCAACGTACTCCACCACATACCGACACCATCCGCCGCTCACCCAAAAAATTGCACACAGCACACCATCACTGCTCACG
TCCCATGGTCACTTAATCCG

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MHPKNFQHQLKTIPQPPEETRDFVFNHTMLRVKDINASLDFYARILGFRLIDQRDFPEAQFSLYFLALLPQTVHISDNDT
ERRLWMSGIPGVLELTHNYGTETQEGQIYHNGNSEPRGFGHICISVPDLYSACARFDTLQVPYQKRLTDGRMKNIAFIKD
PDGYWVEIISNTPLP

Sequences:

>Translated_175_residues
MHPKNFQHQLKTIPQPPEETRDFVFNHTMLRVKDINASLDFYARILGFRLIDQRDFPEAQFSLYFLALLPQTVHISDNDT
ERRLWMSGIPGVLELTHNYGTETQEGQIYHNGNSEPRGFGHICISVPDLYSACARFDTLQVPYQKRLTDGRMKNIAFIKD
PDGYWVEIISNTPLP
>Mature_175_residues
MHPKNFQHQLKTIPQPPEETRDFVFNHTMLRVKDINASLDFYARILGFRLIDQRDFPEAQFSLYFLALLPQTVHISDNDT
ERRLWMSGIPGVLELTHNYGTETQEGQIYHNGNSEPRGFGHICISVPDLYSACARFDTLQVPYQKRLTDGRMKNIAFIKD
PDGYWVEIISNTPLP

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=151, Percent_Identity=56.2913907284768, Blast_Score=178, Evalue=3e-45,
Organism=Escherichia coli, GI1787940, Length=145, Percent_Identity=33.1034482758621, Blast_Score=80, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323639, Length=149, Percent_Identity=45.6375838926174, Blast_Score=134, Evalue=6e-33,
Organism=Drosophila melanogaster, GI20129743, Length=156, Percent_Identity=51.9230769230769, Blast_Score=169, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 20296; Mature: 20296

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00934 GLYOXALASE_I_1 ; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPKNFQHQLKTIPQPPEETRDFVFNHTMLRVKDINASLDFYARILGFRLIDQRDFPEAQ
CCCCCHHHHHHCCCCCCHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHH
FSLYFLALLPQTVHISDNDTERRLWMSGIPGVLELTHNYGTETQEGQIYHNGNSEPRGFG
HHHHHEECCCCEEEECCCCCHHEEECCCCCCEEEEECCCCCCCCCCEEEECCCCCCCCCE
HICISVPDLYSACARFDTLQVPYQKRLTDGRMKNIAFIKDPDGYWVEIISNTPLP
EEEEECCHHHHHHHHCCCCCCCHHHHCCCCCCCCEEEEECCCCEEEEEECCCCCC
>Mature Secondary Structure
MHPKNFQHQLKTIPQPPEETRDFVFNHTMLRVKDINASLDFYARILGFRLIDQRDFPEAQ
CCCCCHHHHHHCCCCCCHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHH
FSLYFLALLPQTVHISDNDTERRLWMSGIPGVLELTHNYGTETQEGQIYHNGNSEPRGFG
HHHHHEECCCCEEEECCCCCHHEEECCCCCCEEEEECCCCCCCCCCEEEECCCCCCCCCE
HICISVPDLYSACARFDTLQVPYQKRLTDGRMKNIAFIKDPDGYWVEIISNTPLP
EEEEECCHHHHHHHHCCCCCCCHHHHCCCCCCCCEEEEECCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7959071 [H]