Click here to switch to the map view.

The map label for this gene is ispB [H]

Identifier: 182681213

GI number: 182681213

Start: 781649

End: 782650

Strand: Reverse

Name: ispB [H]

Synonym: XfasM23_0657

Alternate gene names: 182681213

Gene position: 782650-781649 (Counterclockwise)

Preceding gene: 182681218

Following gene: 182681212

Centisome position: 30.87

GC content: 54.49

Gene sequence:

>1002_bases
ATGAGCATTGCCACCACTCCTCTCCATACCGCCCTAAGCCTTCCCCAGATTCAATCCTTCGCAGCATCAGACATGGCTGC
AATCGATACGCTGATCCACGACCGACTGACCTCAGACATCGTGCTGATCAATCAAATTGCCGATCACATCATCTGCTCAG
GTGGCAAACGCCTGCGCCCCATGCTAACGATGTTAGCTGGCCACGCCATCGGCCCCACAGGCCCTCATCACCACCAACTG
GCTGCCATCATTGAGTTCATCCACACCGCCACACTGCTACATGATGATGTGGTCGACGGATCGGAACTGCGCCGCAGTCG
CAGTACCGCAAATGCACTATGGGGCAATGCACCCAGCGTTCTAGTCGGTGATTTTCTATATTCGCGTAGCTTCCAACTGA
TGACCGAATTAGCCAACCTAGAAGTCATGCAGATATTGGCTGAAACCACCAACCGTATCTCTGAAGGCGAAGTACTGCAA
TTGTTGCACGTACACAACCCTGATACCGACGAAACTACCTACCTAAGCGTGATCGAGCGCAAAACGGCAATATTGTTTGC
TGCCGCAGCACAACTCGGAGCACTCGCATCCCATGCCGATACGCAGACACAGACACGGATGTACGAATATGGAATGCAAT
TGGGCTATGCATTCCAAATCACCGACGACGTACTCGACTACACCGCCAACAATAGCCAACTGGGCAAAAATCTCGGTGAC
GATCTCGCCGAAGGCAAAGCCACCTTACCAGTGATCCATGCGATGACACACTCAGACGCAGCCACATGTGAACGTTTGCG
CATGATCATCAGCAAAGGCGATAGCGATGCAATGCCAGAAGTGCTGAACGCGATCCGTACAACTGGCAGCTTGGACTACA
GTCGGCAACGCGCCAGCGCGTATGCGGCGACCGCCGAACGCGCACTAGACGGCCTGCCCGAAACCGAAGCAGTTGCCGCG
TTGCGCGGCTTGGCCCGCTACGCTGTCCAACGAACCTACTGA

Upstream 100 bases:

>100_bases
AAACCCAACGGCAAGCAAATCAGAACAGAAGCGCTTCCACTTTCAGAACGGATCGCGTGGAGACGCCTATAATTCAGACA
ACACGTACCTTCTGTTCCGC

Downstream 100 bases:

>100_bases
AGTATCGGCGACTTAAACACCGGAGATTGACATCGCCCAAGAAAGAACCCGATCCATCTCACATTATCTAGATCTAGAGA
AACTATTCGATCCGGGCAGA

Product: polyprenyl synthetase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MSIATTPLHTALSLPQIQSFAASDMAAIDTLIHDRLTSDIVLINQIADHIICSGGKRLRPMLTMLAGHAIGPTGPHHHQL
AAIIEFIHTATLLHDDVVDGSELRRSRSTANALWGNAPSVLVGDFLYSRSFQLMTELANLEVMQILAETTNRISEGEVLQ
LLHVHNPDTDETTYLSVIERKTAILFAAAAQLGALASHADTQTQTRMYEYGMQLGYAFQITDDVLDYTANNSQLGKNLGD
DLAEGKATLPVIHAMTHSDAATCERLRMIISKGDSDAMPEVLNAIRTTGSLDYSRQRASAYAATAERALDGLPETEAVAA
LRGLARYAVQRTY

Sequences:

>Translated_333_residues
MSIATTPLHTALSLPQIQSFAASDMAAIDTLIHDRLTSDIVLINQIADHIICSGGKRLRPMLTMLAGHAIGPTGPHHHQL
AAIIEFIHTATLLHDDVVDGSELRRSRSTANALWGNAPSVLVGDFLYSRSFQLMTELANLEVMQILAETTNRISEGEVLQ
LLHVHNPDTDETTYLSVIERKTAILFAAAAQLGALASHADTQTQTRMYEYGMQLGYAFQITDDVLDYTANNSQLGKNLGD
DLAEGKATLPVIHAMTHSDAATCERLRMIISKGDSDAMPEVLNAIRTTGSLDYSRQRASAYAATAERALDGLPETEAVAA
LRGLARYAVQRTY
>Mature_332_residues
SIATTPLHTALSLPQIQSFAASDMAAIDTLIHDRLTSDIVLINQIADHIICSGGKRLRPMLTMLAGHAIGPTGPHHHQLA
AIIEFIHTATLLHDDVVDGSELRRSRSTANALWGNAPSVLVGDFLYSRSFQLMTELANLEVMQILAETTNRISEGEVLQL
LHVHNPDTDETTYLSVIERKTAILFAAAAQLGALASHADTQTQTRMYEYGMQLGYAFQITDDVLDYTANNSQLGKNLGDD
LAEGKATLPVIHAMTHSDAATCERLRMIISKGDSDAMPEVLNAIRTTGSLDYSRQRASAYAATAERALDGLPETEAVAAL
RGLARYAVQRTY

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=244, Percent_Identity=31.9672131147541, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI4758430, Length=279, Percent_Identity=25.089605734767, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI83700220, Length=279, Percent_Identity=25.089605734767, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1789578, Length=321, Percent_Identity=49.8442367601246, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1786623, Length=224, Percent_Identity=31.25, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17505681, Length=333, Percent_Identity=29.1291291291291, Blast_Score=145, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6319475, Length=302, Percent_Identity=29.8013245033113, Blast_Score=125, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6325188, Length=289, Percent_Identity=24.2214532871972, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24651612, Length=297, Percent_Identity=29.96632996633, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI281365769, Length=257, Percent_Identity=25.6809338521401, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24660002, Length=257, Percent_Identity=25.6809338521401, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36164; Mature: 36033

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIATTPLHTALSLPQIQSFAASDMAAIDTLIHDRLTSDIVLINQIADHIICSGGKRLRP
CCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
MLTMLAGHAIGPTGPHHHQLAAIIEFIHTATLLHDDVVDGSELRRSRSTANALWGNAPSV
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHH
LVGDFLYSRSFQLMTELANLEVMQILAETTNRISEGEVLQLLHVHNPDTDETTYLSVIER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHEECCCCCCCHHHHHHHHHH
KTAILFAAAAQLGALASHADTQTQTRMYEYGMQLGYAFQITDDVLDYTANNSQLGKNLGD
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCEEEEEHHHHHHHCCCCHHHHHHHHH
DLAEGKATLPVIHAMTHSDAATCERLRMIISKGDSDAMPEVLNAIRTTGSLDYSRQRASA
HHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
YAATAERALDGLPETEAVAALRGLARYAVQRTY
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIATTPLHTALSLPQIQSFAASDMAAIDTLIHDRLTSDIVLINQIADHIICSGGKRLRP
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
MLTMLAGHAIGPTGPHHHQLAAIIEFIHTATLLHDDVVDGSELRRSRSTANALWGNAPSV
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHH
LVGDFLYSRSFQLMTELANLEVMQILAETTNRISEGEVLQLLHVHNPDTDETTYLSVIER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHEECCCCCCCHHHHHHHHHH
KTAILFAAAAQLGALASHADTQTQTRMYEYGMQLGYAFQITDDVLDYTANNSQLGKNLGD
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCEEEEEHHHHHHHCCCCHHHHHHHHH
DLAEGKATLPVIHAMTHSDAATCERLRMIISKGDSDAMPEVLNAIRTTGSLDYSRQRASA
HHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
YAATAERALDGLPETEAVAALRGLARYAVQRTY
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]