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The map label for this gene is cysW [H]

Identifier: 182681178

GI number: 182681178

Start: 730956

End: 731915

Strand: Direct

Name: cysW [H]

Synonym: XfasM23_0620

Alternate gene names: 182681178

Gene position: 730956-731915 (Clockwise)

Preceding gene: 182681177

Following gene: 182681179

Centisome position: 28.83

GC content: 52.92

Gene sequence:

>960_bases
ATGAGCAAGGATATGATGTCTGGGCCTTCTCTCTGTGTTCAGGGGCAGAGGGGTGTACAGCGTTGGCATCGGTTGTTGAC
CGCATCTGCGACGACCGAGTCGTTGTGGGTACAGTGGTTGCTTATTCTGGGTGCGCTTGGCTTTTTATTGGCGTTTTTAC
TGCTGCCACTGATGCTGGTGTTTTCCGAAGCATTGCGTGGTGGGGTGCAGGTTTTTCTACGGGCGGTCTCTGATCCTGAT
GCGCTGGCTGCAATTCGTTTAACGATGTTAGTAACAATGATTGTGTTGCCGGTGAACATGGTTTTCGGTGTCGCCGTGGC
GTGGGCGGTCAGTAAGTATCAGTTTCCTGGCAAGCGGCTGTTGGTTAGTTTGATTGATTTGCCGTTTTCGGTTTCACCGG
TGGTTGCTGGTTTGGTGTTCGTGCTCATTTTTGGTCGGCAGGGGTGGGCTTGGCCGTTGATTGAGGATGGAGTTGACTTC
TCCTTGCCTTTGTTTGGCGAGGTTATCGTGAAGTTGCCACGGGTCGTGTTCGCGTTGCCGGGGATCGTCTTGGCGACAAT
ATTTGTCACTTTGCCATTTATTGCGCGCGAGCTGATGCCATTGATGGAGCAGCAAGGCAATGAGGAGGAGTTGGCTGCGT
TGAGTCTGGGGGCCAGTGGTTGGCAGACTTTTTGGCGGGTGACGTTACCGAACATTCGTTGGGGGCTGTTGTATGGGGCG
TTGTTGTGTGGTGCGCGTGCGATGGGTGAGTTTGGTGCAGTCTCTGTGGTATCTGGTCACATTCGTGGGCGAACCAACAC
AATGCCGTTGCACGTGGAAATTCTGTATAGCGAATATGCTTACAGTGCTGCATTTGCCTGTGCTTCGTTGTTGGCGTTGA
GTGCGTTGTTGACGTTGGCGTTAAAGGCTTTTCTGGAAAAGCGTCACGGTGAGTTGTTGGCTGTCGGGCGCCAGCATTGA

Upstream 100 bases:

>100_bases
CTGCAATTGCGGCAGTAATGTTGTTGTTGTCATTCACGGTGTTGTTAATGGTCAACGCGATGCAGGCATGCTTATTGCGT
GTTAAACATCGGGGGGGAAG

Downstream 100 bases:

>100_bases
GGATGAATTGAGTATGGGGATCAGAATCCAGGAGCTGCGTAAACAGTTTGAGGATTTCACTGCATTGGCGGGTATCGATC
TTGATATTCGTCAGGGTGAA

Product: sulfate ABC transporter inner membrane protein CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MSKDMMSGPSLCVQGQRGVQRWHRLLTASATTESLWVQWLLILGALGFLLAFLLLPLMLVFSEALRGGVQVFLRAVSDPD
ALAAIRLTMLVTMIVLPVNMVFGVAVAWAVSKYQFPGKRLLVSLIDLPFSVSPVVAGLVFVLIFGRQGWAWPLIEDGVDF
SLPLFGEVIVKLPRVVFALPGIVLATIFVTLPFIARELMPLMEQQGNEEELAALSLGASGWQTFWRVTLPNIRWGLLYGA
LLCGARAMGEFGAVSVVSGHIRGRTNTMPLHVEILYSEYAYSAAFACASLLALSALLTLALKAFLEKRHGELLAVGRQH

Sequences:

>Translated_319_residues
MSKDMMSGPSLCVQGQRGVQRWHRLLTASATTESLWVQWLLILGALGFLLAFLLLPLMLVFSEALRGGVQVFLRAVSDPD
ALAAIRLTMLVTMIVLPVNMVFGVAVAWAVSKYQFPGKRLLVSLIDLPFSVSPVVAGLVFVLIFGRQGWAWPLIEDGVDF
SLPLFGEVIVKLPRVVFALPGIVLATIFVTLPFIARELMPLMEQQGNEEELAALSLGASGWQTFWRVTLPNIRWGLLYGA
LLCGARAMGEFGAVSVVSGHIRGRTNTMPLHVEILYSEYAYSAAFACASLLALSALLTLALKAFLEKRHGELLAVGRQH
>Mature_318_residues
SKDMMSGPSLCVQGQRGVQRWHRLLTASATTESLWVQWLLILGALGFLLAFLLLPLMLVFSEALRGGVQVFLRAVSDPDA
LAAIRLTMLVTMIVLPVNMVFGVAVAWAVSKYQFPGKRLLVSLIDLPFSVSPVVAGLVFVLIFGRQGWAWPLIEDGVDFS
LPLFGEVIVKLPRVVFALPGIVLATIFVTLPFIARELMPLMEQQGNEEELAALSLGASGWQTFWRVTLPNIRWGLLYGAL
LCGARAMGEFGAVSVVSGHIRGRTNTMPLHVEILYSEYAYSAAFACASLLALSALLTLALKAFLEKRHGELLAVGRQH

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=288, Percent_Identity=47.5694444444444, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI1788764, Length=241, Percent_Identity=34.0248962655602, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1786980, Length=196, Percent_Identity=31.6326530612245, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34834; Mature: 34703

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDMMSGPSLCVQGQRGVQRWHRLLTASATTESLWVQWLLILGALGFLLAFLLLPLMLV
CCCCCCCCCCCEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSEALRGGVQVFLRAVSDPDALAAIRLTMLVTMIVLPVNMVFGVAVAWAVSKYQFPGKRL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LVSLIDLPFSVSPVVAGLVFVLIFGRQGWAWPLIEDGVDFSLPLFGEVIVKLPRVVFALP
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHH
GIVLATIFVTLPFIARELMPLMEQQGNEEELAALSLGASGWQTFWRVTLPNIRWGLLYGA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHEECCCCHHHHHHHH
LLCGARAMGEFGAVSVVSGHIRGRTNTMPLHVEILYSEYAYSAAFACASLLALSALLTLA
HHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
LKAFLEKRHGELLAVGRQH
HHHHHHHHCCCEEECCCCC
>Mature Secondary Structure 
SKDMMSGPSLCVQGQRGVQRWHRLLTASATTESLWVQWLLILGALGFLLAFLLLPLMLV
CCCCCCCCCCEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSEALRGGVQVFLRAVSDPDALAAIRLTMLVTMIVLPVNMVFGVAVAWAVSKYQFPGKRL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LVSLIDLPFSVSPVVAGLVFVLIFGRQGWAWPLIEDGVDFSLPLFGEVIVKLPRVVFALP
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHH
GIVLATIFVTLPFIARELMPLMEQQGNEEELAALSLGASGWQTFWRVTLPNIRWGLLYGA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHEECCCCHHHHHHHH
LLCGARAMGEFGAVSVVSGHIRGRTNTMPLHVEILYSEYAYSAAFACASLLALSALLTLA
HHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
LKAFLEKRHGELLAVGRQH
HHHHHHHHCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]