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The map label for this gene is cysU [H]

Identifier: 182681177

GI number: 182681177

Start: 730099

End: 730959

Strand: Direct

Name: cysU [H]

Synonym: XfasM23_0619

Alternate gene names: 182681177

Gene position: 730099-730959 (Clockwise)

Preceding gene: 182681176

Following gene: 182681178

Centisome position: 28.79

GC content: 54.59

Gene sequence:

>861_bases
ATGGCTGAAAGTACTGTGGCGCCGGCGCGTTTGTCACGTCGGCGCGTGATTCCTGGCCTTGGTTTGAGTCTTGGAATCAC
CTTTGTTTGGTTGGGGCTGATCGTGCTGATTCCGCTGGTTGGCGTATTTGTCAAGGCCAGTGGGTTGGGATGGAGTGGGC
TATGGAAGGTATGGACGGAACCACGGGTGCTGTCTGCGTTGTCGGTCAGTTTTGGCGCTGCGTTAGTTGCGGCGGCGTTT
AACGCGGTGATGGGGACGTGGGTAGCTTGGGTGTTTGGGCGTTACGATTTTCCTGGTAAGCGTGTTTTCGATGCGATCAT
TGATTTACCGTTCGCATTGCCGACAGCAGTGGCCGGTATTGCGCTGGCGGCATTGTATGGACCCAATGGATGGATAGGGC
GTTGGCTGGAGCTGGCTGGGTTCAAGGTTGCTTACACTCCGTTGGGGATCGTAGTAGCGCTGGTGTTCGTTGGTCTGCCT
TTTGTGGTGCGGATTGTCCAGCCGGTGTTGGCAGAGGCCGAGAAGGAGTTGGAGGAGGCTGCTGCTTCACTCGGTGCGAA
TCGTTGGCAGACGATGTTCCGTGTCGTATTGCCGACGCTATGGCCTGCAATGTTGACGGGATTTGCGTTGGCTTTCGCGC
GCGGTGTGGGTGAGTATGGGTCGGTGATTTTTATTGCTGGCAATCTACCCAATGCAACTGAGATCGCGCCGTTGCTCATC
ACGATTCGTTTGGAGGAGTTTGACTATTCTGGAGCTACTGCAATTGCGGCAGTAATGTTGTTGTTGTCATTCACGGTGTT
GTTAATGGTCAACGCGATGCAGGCATGCTTATTGCGTGTTAAACATCGGGGGGGAAGATGA

Upstream 100 bases:

>100_bases
GGTGACGATCGGGCGAGCGTTCGGTTCTTGGGCTAAGGTGCAGCAGGAGCATTTTACCGATGGTGGTGTGTTCGACCAGA
TCCAGGCGGGAAAGTAGTCC

Downstream 100 bases:

>100_bases
GCAAGGATATGATGTCTGGGCCTTCTCTCTGTGTTCAGGGGCAGAGGGGTGTACAGCGTTGGCATCGGTTGTTGACCGCA
TCTGCGACGACCGAGTCGTT

Product: sulfate ABC transporter inner membrane protein CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MAESTVAPARLSRRRVIPGLGLSLGITFVWLGLIVLIPLVGVFVKASGLGWSGLWKVWTEPRVLSALSVSFGAALVAAAF
NAVMGTWVAWVFGRYDFPGKRVFDAIIDLPFALPTAVAGIALAALYGPNGWIGRWLELAGFKVAYTPLGIVVALVFVGLP
FVVRIVQPVLAEAEKELEEAAASLGANRWQTMFRVVLPTLWPAMLTGFALAFARGVGEYGSVIFIAGNLPNATEIAPLLI
TIRLEEFDYSGATAIAAVMLLLSFTVLLMVNAMQACLLRVKHRGGR

Sequences:

>Translated_286_residues
MAESTVAPARLSRRRVIPGLGLSLGITFVWLGLIVLIPLVGVFVKASGLGWSGLWKVWTEPRVLSALSVSFGAALVAAAF
NAVMGTWVAWVFGRYDFPGKRVFDAIIDLPFALPTAVAGIALAALYGPNGWIGRWLELAGFKVAYTPLGIVVALVFVGLP
FVVRIVQPVLAEAEKELEEAAASLGANRWQTMFRVVLPTLWPAMLTGFALAFARGVGEYGSVIFIAGNLPNATEIAPLLI
TIRLEEFDYSGATAIAAVMLLLSFTVLLMVNAMQACLLRVKHRGGR
>Mature_285_residues
AESTVAPARLSRRRVIPGLGLSLGITFVWLGLIVLIPLVGVFVKASGLGWSGLWKVWTEPRVLSALSVSFGAALVAAAFN
AVMGTWVAWVFGRYDFPGKRVFDAIIDLPFALPTAVAGIALAALYGPNGWIGRWLELAGFKVAYTPLGIVVALVFVGLPF
VVRIVQPVLAEAEKELEEAAASLGANRWQTMFRVVLPTLWPAMLTGFALAFARGVGEYGSVIFIAGNLPNATEIAPLLIT
IRLEEFDYSGATAIAAVMLLLSFTVLLMVNAMQACLLRVKHRGGR

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=265, Percent_Identity=50.188679245283, Blast_Score=244, Evalue=5e-66,
Organism=Escherichia coli, GI87082099, Length=286, Percent_Identity=31.8181818181818, Blast_Score=147, Evalue=6e-37,
Organism=Escherichia coli, GI1786980, Length=232, Percent_Identity=31.0344827586207, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786254, Length=215, Percent_Identity=26.5116279069767, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787369, Length=165, Percent_Identity=24.2424242424242, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30640; Mature: 30509

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AESTVAPARLSRRRVIPGLGLSLGITFVWLGLIVLIPLVGVFVKASGLGWSGLWKVWTE
CCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
PRVLSALSVSFGAALVAAAFNAVMGTWVAWVFGRYDFPGKRVFDAIIDLPFALPTAVAGI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHH
ALAALYGPNGWIGRWLELAGFKVAYTPLGIVVALVFVGLPFVVRIVQPVLAEAEKELEEA
HHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AASLGANRWQTMFRVVLPTLWPAMLTGFALAFARGVGEYGSVIFIAGNLPNATEIAPLLI
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHE
TIRLEEFDYSGATAIAAVMLLLSFTVLLMVNAMQACLLRVKHRGGR
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]