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The map label for this gene is acnA [H]

Identifier: 182681091

GI number: 182681091

Start: 630835

End: 632709

Strand: Direct

Name: acnA [H]

Synonym: XfasM23_0533

Alternate gene names: 182681091

Gene position: 630835-632709 (Clockwise)

Preceding gene: 182681090

Following gene: 182681092

Centisome position: 24.88

GC content: 59.31

Gene sequence:

>1875_bases
ATGCACAACCGCTATTGCAAACCCCTGCCGGGGACACCGTTCCATTACTACGATGCTCGGGCCGCCATTGATGCGTTGTG
CCCCGGGGCTTACGCGGCGTTGCCATATGTCGCTCGCGTGTATGCAGAAAACGTGTTGCGCCGCGCCGTGTCGGATCAGT
GGCCTGCGTATCTGGGCCAGCTCATTGGGCGGCGCTGTGATACCGATATCCCGTGGTTTCCAGCGCGTATCGTCTGCCAT
GACATCCTTGGCCTGACAGCACTGGTTGATTTGGCTGGATTGCGTGATGCGATTGCGGCCCAGGGAGGGGATGCCTTCCA
GGTCAACCCCACGGTGCCGGTACAACTGATTGTTGACCACTCATTGGCGGTGGAATACGGCGGCAGCGATCCGGAGGCGG
TTGCCAAAAACCGTGCGATTGAAGCACGCCGCAACGCGGATCGATTTCATTTTATCGAGTGGACCCGACACGCTTTTGAA
AATGTCACGGTGATTCCTCCGGGGTACGGCATCATGCATCAAATCAACCTGGAGGCACTGTCGCCTGTCGTCGCAGTCCA
GGATGGCGTGGCGTTCCCAGATACCTGTATCGGTACAGATAGCCATACCCCGCATGTGAATGCTCTGGGTGTGGTTGCCA
TTGGTGTTGGCGGCTTAGAGGCGGAGAGTGTGATGCTGGGCCGTGCGGTGTGGCTGCGTTGTCCAGAGAGTGTGGGTGTG
GAGTTACACGGACGCCCGCGCCCCGGGATCACCGCAACGGATATTGTGCTAGCGCTGACAGCGTTTTTACGCCAGGCGCG
TGTGGTGGGTGCGTATCTGGAATTCCGTGGCGAAGGCGTCCCTGCGCTCACCGTGGGTGACCGTGCCACGATTGCCAACA
TGGCTCCGGAATATGGCGCAACTGCGGCGTTGTTCTTTATTGATGCGCAGACCGTGGAGTATTTACGGCTGACCGGACGC
GACAAGGCACAGGTGGCGTTGGTCGATACCTACGCCAAACAGGCCGGACTGTGGGCCGATACGCTTGATGGGGCACAGTA
CGCGCGCCTGCTGCATTTCGACCTCTCCAGCGTCGCACGCACCATGGCTGGCCCGTCCAGCCCACATCACCAATTGCCCA
CATCCGCGTTGACGGAGCGCGGCATTGCTGGCGGCTTGGCGCAAGCGCGGGCAGAGGAAGCCGCCGGGCATCTGCCCGAT
GGCGCAGTCATCATTGCGGCAATCACCTCATGCACCAATACCTCTAACCCGCGTAACCTCATCGCTGCCGGTTTATTGGC
GCGTAATGCTCATGCTCGGGGACTACGCTGTAAACCTTGGGTCAAAACCTCCCTAGCGCCGGGTTCCCAAGCGGTGCCAT
TGTATTTGGAGGCGGCCGGACTATTGTCCGCGCTACAGCAGCTTGGCTTTGGCGTCGTCGGTTTTGCTTGCACCACCTGC
AATGGCATGAGTGGTGCACTCGATCCGCTGATCCAGCACGCCATCATGGCGCGTCATGTGCACACCGTGGCCGTGTTGTC
GGGGAACCGCAACTTTGACGGACGCATTCATCCTGATGCCACACAAGCTTTTTTGGCTTCGCCGCCTCTGGTGATTGCCT
ATGCAATCGCTGGCACCATCCGTTTTGATATTGAACGAGATGTGCTCGGTCATGATGCCGATGGTACCCCGGTGATGCTC
AAAGATATTTGGCCCAGTGATGCCGACATTGATGCACTGGTGCGTCGCAGCGTTCACCCGGAGCAATTCCGTCATGTGTA
CATGCCGCTGTTGCAAGCGTCTAGACAGCGGCGTGAATGCGTCCCGCCGTTATACGCATGGGGCACCCCGGGAGTACGTA
CATCCGTCGGCCCCCTTATTGGGAAGGGGTCTTAG

Upstream 100 bases:

>100_bases
ACCCATGATGTTGTCCTTTGATGGCTCCCAAAGGGGGGCGTTATCACCCCATCGACACTCGCGCTATGTAGACTACACAG
CAGCCGCAGTGCCTTCCCCT

Downstream 100 bases:

>100_bases
CCGGTGCGCGCACCTTGCGCGGGTTACGGCCGTTGGCGGTGTTGGGCGATAACATCACCACCGATCATCTGTCTCCGTCG
AATGCCATCCTGCCCAATAG

Product: aconitate hydratase domain-containing protein

Products: NA

Alternate protein names: Aconitase; Citrate hydro-lyase [H]

Number of amino acids: Translated: 624; Mature: 624

Protein sequence:

>624_residues
MHNRYCKPLPGTPFHYYDARAAIDALCPGAYAALPYVARVYAENVLRRAVSDQWPAYLGQLIGRRCDTDIPWFPARIVCH
DILGLTALVDLAGLRDAIAAQGGDAFQVNPTVPVQLIVDHSLAVEYGGSDPEAVAKNRAIEARRNADRFHFIEWTRHAFE
NVTVIPPGYGIMHQINLEALSPVVAVQDGVAFPDTCIGTDSHTPHVNALGVVAIGVGGLEAESVMLGRAVWLRCPESVGV
ELHGRPRPGITATDIVLALTAFLRQARVVGAYLEFRGEGVPALTVGDRATIANMAPEYGATAALFFIDAQTVEYLRLTGR
DKAQVALVDTYAKQAGLWADTLDGAQYARLLHFDLSSVARTMAGPSSPHHQLPTSALTERGIAGGLAQARAEEAAGHLPD
GAVIIAAITSCTNTSNPRNLIAAGLLARNAHARGLRCKPWVKTSLAPGSQAVPLYLEAAGLLSALQQLGFGVVGFACTTC
NGMSGALDPLIQHAIMARHVHTVAVLSGNRNFDGRIHPDATQAFLASPPLVIAYAIAGTIRFDIERDVLGHDADGTPVML
KDIWPSDADIDALVRRSVHPEQFRHVYMPLLQASRQRRECVPPLYAWGTPGVRTSVGPLIGKGS

Sequences:

>Translated_624_residues
MHNRYCKPLPGTPFHYYDARAAIDALCPGAYAALPYVARVYAENVLRRAVSDQWPAYLGQLIGRRCDTDIPWFPARIVCH
DILGLTALVDLAGLRDAIAAQGGDAFQVNPTVPVQLIVDHSLAVEYGGSDPEAVAKNRAIEARRNADRFHFIEWTRHAFE
NVTVIPPGYGIMHQINLEALSPVVAVQDGVAFPDTCIGTDSHTPHVNALGVVAIGVGGLEAESVMLGRAVWLRCPESVGV
ELHGRPRPGITATDIVLALTAFLRQARVVGAYLEFRGEGVPALTVGDRATIANMAPEYGATAALFFIDAQTVEYLRLTGR
DKAQVALVDTYAKQAGLWADTLDGAQYARLLHFDLSSVARTMAGPSSPHHQLPTSALTERGIAGGLAQARAEEAAGHLPD
GAVIIAAITSCTNTSNPRNLIAAGLLARNAHARGLRCKPWVKTSLAPGSQAVPLYLEAAGLLSALQQLGFGVVGFACTTC
NGMSGALDPLIQHAIMARHVHTVAVLSGNRNFDGRIHPDATQAFLASPPLVIAYAIAGTIRFDIERDVLGHDADGTPVML
KDIWPSDADIDALVRRSVHPEQFRHVYMPLLQASRQRRECVPPLYAWGTPGVRTSVGPLIGKGS
>Mature_624_residues
MHNRYCKPLPGTPFHYYDARAAIDALCPGAYAALPYVARVYAENVLRRAVSDQWPAYLGQLIGRRCDTDIPWFPARIVCH
DILGLTALVDLAGLRDAIAAQGGDAFQVNPTVPVQLIVDHSLAVEYGGSDPEAVAKNRAIEARRNADRFHFIEWTRHAFE
NVTVIPPGYGIMHQINLEALSPVVAVQDGVAFPDTCIGTDSHTPHVNALGVVAIGVGGLEAESVMLGRAVWLRCPESVGV
ELHGRPRPGITATDIVLALTAFLRQARVVGAYLEFRGEGVPALTVGDRATIANMAPEYGATAALFFIDAQTVEYLRLTGR
DKAQVALVDTYAKQAGLWADTLDGAQYARLLHFDLSSVARTMAGPSSPHHQLPTSALTERGIAGGLAQARAEEAAGHLPD
GAVIIAAITSCTNTSNPRNLIAAGLLARNAHARGLRCKPWVKTSLAPGSQAVPLYLEAAGLLSALQQLGFGVVGFACTTC
NGMSGALDPLIQHAIMARHVHTVAVLSGNRNFDGRIHPDATQAFLASPPLVIAYAIAGTIRFDIERDVLGHDADGTPVML
KDIWPSDADIDALVRRSVHPEQFRHVYMPLLQASRQRRECVPPLYAWGTPGVRTSVGPLIGKGS

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate [H]

COG id: COG1048

COG function: function code C; Aconitase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Homo sapiens, GI8659555, Length=629, Percent_Identity=41.1764705882353, Blast_Score=487, Evalue=1e-137,
Organism=Homo sapiens, GI41352693, Length=458, Percent_Identity=42.3580786026201, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI4501867, Length=506, Percent_Identity=30.4347826086957, Blast_Score=155, Evalue=8e-38,
Organism=Escherichia coli, GI1787531, Length=604, Percent_Identity=45.364238410596, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI1786259, Length=367, Percent_Identity=28.6103542234332, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI87081781, Length=389, Percent_Identity=25.706940874036, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17568399, Length=625, Percent_Identity=41.12, Blast_Score=474, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI25149337, Length=455, Percent_Identity=30.7692307692308, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI32564738, Length=455, Percent_Identity=30.7692307692308, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI25149342, Length=334, Percent_Identity=28.7425149700599, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323335, Length=528, Percent_Identity=32.0075757575758, Blast_Score=185, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6322261, Length=437, Percent_Identity=29.9771167048055, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6321429, Length=360, Percent_Identity=27.2222222222222, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320440, Length=397, Percent_Identity=23.4256926952141, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24645686, Length=620, Percent_Identity=40.9677419354839, Blast_Score=461, Evalue=1e-129,
Organism=Drosophila melanogaster, GI17137564, Length=636, Percent_Identity=40.5660377358491, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI161076999, Length=452, Percent_Identity=30.7522123893805, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI281365315, Length=452, Percent_Identity=30.7522123893805, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI17864292, Length=452, Percent_Identity=30.7522123893805, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI28571643, Length=454, Percent_Identity=30.3964757709251, Blast_Score=148, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR006249
- InterPro:   IPR015934
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00330 Aconitase; PF00694 Aconitase_C [H]

EC number: =4.2.1.3 [H]

Molecular weight: Translated: 66761; Mature: 66761

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNRYCKPLPGTPFHYYDARAAIDALCPGAYAALPYVARVYAENVLRRAVSDQWPAYLGQ
CCCCCCCCCCCCCCEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LIGRRCDTDIPWFPARIVCHDILGLTALVDLAGLRDAIAAQGGDAFQVNPTVPVQLIVDH
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEC
SLAVEYGGSDPEAVAKNRAIEARRNADRFHFIEWTRHAFENVTVIPPGYGIMHQINLEAL
EEEEEECCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCEEEECCCCCCEEEECHHHH
SPVVAVQDGVAFPDTCIGTDSHTPHVNALGVVAIGVGGLEAESVMLGRAVWLRCPESVGV
CCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHEEECCHHCCE
ELHGRPRPGITATDIVLALTAFLRQARVVGAYLEFRGEGVPALTVGDRATIANMAPEYGA
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHCCCCCCC
TAALFFIDAQTVEYLRLTGRDKAQVALVDTYAKQAGLWADTLDGAQYARLLHFDLSSVAR
EEEEEEEEHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHH
TMAGPSSPHHQLPTSALTERGIAGGLAQARAEEAAGHLPDGAVIIAAITSCTNTSNPRNL
HHCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEEEHHHCCCCCCHHH
IAAGLLARNAHARGLRCKPWVKTSLAPGSQAVPLYLEAAGLLSALQQLGFGVVGFACTTC
HHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEHHHHHCC
NGMSGALDPLIQHAIMARHVHTVAVLSGNRNFDGRIHPDATQAFLASPPLVIAYAIAGTI
CCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCHHHHHHCCCCCEEEEEHHCEE
RFDIERDVLGHDADGTPVMLKDIWPSDADIDALVRRSVHPEQFRHVYMPLLQASRQRREC
EEEEEHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
VPPLYAWGTPGVRTSVGPLIGKGS
CCCCEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHNRYCKPLPGTPFHYYDARAAIDALCPGAYAALPYVARVYAENVLRRAVSDQWPAYLGQ
CCCCCCCCCCCCCCEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LIGRRCDTDIPWFPARIVCHDILGLTALVDLAGLRDAIAAQGGDAFQVNPTVPVQLIVDH
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEC
SLAVEYGGSDPEAVAKNRAIEARRNADRFHFIEWTRHAFENVTVIPPGYGIMHQINLEAL
EEEEEECCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCEEEECCCCCCEEEECHHHH
SPVVAVQDGVAFPDTCIGTDSHTPHVNALGVVAIGVGGLEAESVMLGRAVWLRCPESVGV
CCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHEEECCHHCCE
ELHGRPRPGITATDIVLALTAFLRQARVVGAYLEFRGEGVPALTVGDRATIANMAPEYGA
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHCCCCCCC
TAALFFIDAQTVEYLRLTGRDKAQVALVDTYAKQAGLWADTLDGAQYARLLHFDLSSVAR
EEEEEEEEHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHH
TMAGPSSPHHQLPTSALTERGIAGGLAQARAEEAAGHLPDGAVIIAAITSCTNTSNPRNL
HHCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEEEHHHCCCCCCHHH
IAAGLLARNAHARGLRCKPWVKTSLAPGSQAVPLYLEAAGLLSALQQLGFGVVGFACTTC
HHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEHHHHHCC
NGMSGALDPLIQHAIMARHVHTVAVLSGNRNFDGRIHPDATQAFLASPPLVIAYAIAGTI
CCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCHHHHHHCCCCCEEEEEHHCEE
RFDIERDVLGHDADGTPVMLKDIWPSDADIDALVRRSVHPEQFRHVYMPLLQASRQRREC
EEEEEHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
VPPLYAWGTPGVRTSVGPLIGKGS
CCCCEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA