Click here to switch to the map view.

The map label for this gene is mdh

Identifier: 182681046

GI number: 182681046

Start: 587063

End: 588049

Strand: Reverse

Name: mdh

Synonym: XfasM23_0485

Alternate gene names: 182681046

Gene position: 588049-587063 (Counterclockwise)

Preceding gene: 182681047

Following gene: 182681041

Centisome position: 23.19

GC content: 52.89

Gene sequence:

>987_bases
ATGAAAGCACTTGTTCGTGTTGCTGTGACCGGCGCTGCAGGCCAAATCGGCTACTCATTGCTATTTCGCATCGCTGCCGG
AGAAATGTTTGGCAAAGACAGGCCAGTGATACTCCAGATGCTGGAACTCCCCGACGAGAAAGCACAGGCTGCCCTTAAGG
GCGTGATGATGGAGCTTGAAGACTGCGCGTTCCCACTACTGGCTGGCATGGTCGTTACCGATAATCCAGACATAGCATTC
AAGGATGCCGACGCCGCATTGCTGGTAGGCTCCCGCCCCCGTGGCCCTGGCATGGAACGCAAAGATCTGCTAATGGAAAA
CGCTAAAATTTTTACCGCCCAAGGTGCGGCCTTAAACAAAGTGGCTCGGCGCGATGTCAAAGTGCTGGTCGTTGGTAATC
CAGCAAACACCAACGCCTACATCGCAATGAAATCGGCTCCAGATCTAAACCCGAAACACTTTACTGCGATGCTGCGCCTC
GACCACAACCGCGCATTGAGCCAGTTATCGACCAAGCTAGGTAAGCCTGTCGCCAACATTGAAAAACTCATTGTATGGGG
CAATCACAGTCCAACCATGTATCCGGATTACCGTTTCGCCACCGCCGATGGCACTCCAATCATTGAGGCGATTAACGACC
AAGCATGGAACGCTAACAGCTTCATTCCCACCGTCAGCAAGCGTGGTGCCGCAATCATCGAAGCACGTGGCTTGTCTTCA
GCAGCTTCTGCAGCCAACGCAGCCATTGACCACATGCGTGATTGGCTGCTTGGCAGCAACGGCAAGTGGATCACAATGGG
AGTGCCATCGGACGGTTCCTACGGCATTCCGGAGGGAATGATATTTGGTTTCCCAGTCACGACCACCAATGGCGAATACA
GCATCGTCAAAGATCTGCCCATTGACACCTTCAGCAAAACATACATTGACAAAACGCTGGCCGAACTGGAAGAAGAGCGT
GCCAGTATCGCGCATCTGTTACGTTAA

Upstream 100 bases:

>100_bases
TCCACTTGTTGTATGAACATGCTATCCAACACTTCATTGCCGTTTTCCCACAACACCCCACCAGTTTTAGATCACTTCAT
TGAGCACAGGGAAACATCCT

Downstream 100 bases:

>100_bases
CTGAGATATATCAGCGATTGGGCACCGACTGATGTCCGGGGCCCATTGCTTCCCGATTTACCCGGGCTTTGACATTCTTG
GATTGAGACTGCTCAGCAAA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKALVRVAVTGAAGQIGYSLLFRIAAGEMFGKDRPVILQMLELPDEKAQAALKGVMMELEDCAFPLLAGMVVTDNPDIAF
KDADAALLVGSRPRGPGMERKDLLMENAKIFTAQGAALNKVARRDVKVLVVGNPANTNAYIAMKSAPDLNPKHFTAMLRL
DHNRALSQLSTKLGKPVANIEKLIVWGNHSPTMYPDYRFATADGTPIIEAINDQAWNANSFIPTVSKRGAAIIEARGLSS
AASAANAAIDHMRDWLLGSNGKWITMGVPSDGSYGIPEGMIFGFPVTTTNGEYSIVKDLPIDTFSKTYIDKTLAELEEER
ASIAHLLR

Sequences:

>Translated_328_residues
MKALVRVAVTGAAGQIGYSLLFRIAAGEMFGKDRPVILQMLELPDEKAQAALKGVMMELEDCAFPLLAGMVVTDNPDIAF
KDADAALLVGSRPRGPGMERKDLLMENAKIFTAQGAALNKVARRDVKVLVVGNPANTNAYIAMKSAPDLNPKHFTAMLRL
DHNRALSQLSTKLGKPVANIEKLIVWGNHSPTMYPDYRFATADGTPIIEAINDQAWNANSFIPTVSKRGAAIIEARGLSS
AASAANAAIDHMRDWLLGSNGKWITMGVPSDGSYGIPEGMIFGFPVTTTNGEYSIVKDLPIDTFSKTYIDKTLAELEEER
ASIAHLLR
>Mature_328_residues
MKALVRVAVTGAAGQIGYSLLFRIAAGEMFGKDRPVILQMLELPDEKAQAALKGVMMELEDCAFPLLAGMVVTDNPDIAF
KDADAALLVGSRPRGPGMERKDLLMENAKIFTAQGAALNKVARRDVKVLVVGNPANTNAYIAMKSAPDLNPKHFTAMLRL
DHNRALSQLSTKLGKPVANIEKLIVWGNHSPTMYPDYRFATADGTPIIEAINDQAWNANSFIPTVSKRGAAIIEARGLSS
AASAANAAIDHMRDWLLGSNGKWITMGVPSDGSYGIPEGMIFGFPVTTTNGEYSIVKDLPIDTFSKTYIDKTLAELEEER
ASIAHLLR

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family

Homologues:

Organism=Homo sapiens, GI5174539, Length=333, Percent_Identity=50.1501501501502, Blast_Score=332, Evalue=3e-91,
Organism=Homo sapiens, GI89886456, Length=330, Percent_Identity=23.9393939393939, Blast_Score=105, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17561064, Length=326, Percent_Identity=53.0674846625767, Blast_Score=315, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI32566798, Length=264, Percent_Identity=52.2727272727273, Blast_Score=245, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI32566800, Length=169, Percent_Identity=52.6627218934911, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI24583394, Length=321, Percent_Identity=53.8940809968847, Blast_Score=326, Evalue=1e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MDH_XYLF2 (B2I8M1)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829206.1
- GeneID:   6203658
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0485
- HOGENOM:   HBG289884
- OMA:   MIIWGNH
- ProtClustDB:   PRK05442
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01517
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23382
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01759

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 35428; Mature: 35428

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 189-189 BINDING 94-94 BINDING 100-100 BINDING 107-107 BINDING 114-114 BINDING 133-133 BINDING 164-164

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALVRVAVTGAAGQIGYSLLFRIAAGEMFGKDRPVILQMLELPDEKAQAALKGVMMELE
CCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
DCAFPLLAGMVVTDNPDIAFKDADAALLVGSRPRGPGMERKDLLMENAKIFTAQGAALNK
HHHHHHHCCEEEECCCCEEEECCCEEEEECCCCCCCCCCHHHHHHHCCEEEEECCHHHHH
VARRDVKVLVVGNPANTNAYIAMKSAPDLNPKHFTAMLRLDHNRALSQLSTKLGKPVANI
HHHCCEEEEEECCCCCCCEEEEECCCCCCCHHHEEEEEEECHHHHHHHHHHHHCCCHHCC
EKLIVWGNHSPTMYPDYRFATADGTPIIEAINDQAWNANSFIPTVSKRGAAIIEARGLSS
EEEEEECCCCCCCCCCEEEEECCCCCHHEECCCCCCCCCCCCCCCCCCCCEEEEECCCCH
AASAANAAIDHMRDWLLGSNGKWITMGVPSDGSYGIPEGMIFGFPVTTTNGEYSIVKDLP
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEEEEEECCCCEEEEECCC
IDTFSKTYIDKTLAELEEERASIAHLLR
CCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKALVRVAVTGAAGQIGYSLLFRIAAGEMFGKDRPVILQMLELPDEKAQAALKGVMMELE
CCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
DCAFPLLAGMVVTDNPDIAFKDADAALLVGSRPRGPGMERKDLLMENAKIFTAQGAALNK
HHHHHHHCCEEEECCCCEEEECCCEEEEECCCCCCCCCCHHHHHHHCCEEEEECCHHHHH
VARRDVKVLVVGNPANTNAYIAMKSAPDLNPKHFTAMLRLDHNRALSQLSTKLGKPVANI
HHHCCEEEEEECCCCCCCEEEEECCCCCCCHHHEEEEEEECHHHHHHHHHHHHCCCHHCC
EKLIVWGNHSPTMYPDYRFATADGTPIIEAINDQAWNANSFIPTVSKRGAAIIEARGLSS
EEEEEECCCCCCCCCCEEEEECCCCCHHEECCCCCCCCCCCCCCCCCCCCEEEEECCCCH
AASAANAAIDHMRDWLLGSNGKWITMGVPSDGSYGIPEGMIFGFPVTTTNGEYSIVKDLP
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEEEEEECCCCEEEEECCC
IDTFSKTYIDKTLAELEEERASIAHLLR
CCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA