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The map label for this gene is glmU

Identifier: 182680982

GI number: 182680982

Start: 525417

End: 526790

Strand: Reverse

Name: glmU

Synonym: XfasM23_0421

Alternate gene names: 182680982

Gene position: 526790-525417 (Counterclockwise)

Preceding gene: 182680983

Following gene: 182680977

Centisome position: 20.78

GC content: 54.59

Gene sequence:

>1374_bases
ATGCCTCTGTCCCTACCCCTACACATCGTGATCCTTGCCGCCGGCGAAGGTAAGCGCATGAAATCGGCACTGCCAAAAGT
ACTGCATCCCATCGCCGGAAAACCGATGCTCGCGCATGTGATTACGACCGCACGCGCACTCACTCCAGACGCGATCCACG
TAGTCTACGGCCACGCTGGCAACCAAGTCCGCGCAGCATTTGCAGACCAGACCGACCTACACTGGGTCGAACAGACTCAG
CAACTTGGCACGGGACACGCAGTCAAACAAACGATGTCGGCCATCCCGAATGCAGCAAACGTACTCGTACTGTATGGCGA
CGTACCATTGATTCGTGCCGAAACCCTGCAACGTCTGCCAAGAGCATCTACACCGATCGCCGTACTGGTTACAGAGCTCG
CAAACCCAGCTGGCTACGGCCACATCGTACGCAATAGCGAAGGCAAAGTCGCAGCGATCATTGAAGACAAAGACGCCGAC
GAAGAACAACGCCGCATCCATACTGTCAACACTGGCATCCTCTGTGCTGAATCAACGGCACTACGCCGTTGGCTAAGCAA
ACTATCAAATACCAATATGCAGGGGGAATACTACCTGACCGACATCTTTGCCAGCGCCACAGCCGACCTCACACCAGCCA
ACATGATCATGGTTACCGATGCTCAAGAAGTGGAAGGCGTCAATGATCTTTGGCAACTCACCCAACTGGAACGCGCCTGG
CAAATACGCGCTGCACGTGCCTTGTGCCTGCAAGGCGCACGCGTGGCCGATCCAGCAAGACTGGACCAACGAGGCACAAT
ACGCATCGGACAGAATGTCCACATTGATATTGACGTTGTGCTAGAAGGAGAAATCGAACTCGGTGATAACGTCGTAATTG
GCCCATTCGTGCGACTGAAGAACGTAAAACTTGGACCTGGTACCAAAGTACACGCACACTGTGATCTGGAAGGCGTCACC
ACAACGGGCTCAGCACTCATCGGTCCATTTGCACGCCTGCGTCCGGAAACAATGCTGGCCGACGGCGTGCACATCGGTAA
CTTTGTCGAGACAAAAAACACGTCAATAGGTGCCGATAGTAAAGCCAATCACCTAACTTACTTGGGCGACGCACAAATCG
GCACCAAGGTAAACATTGGTGCTGGCACCATCACTTGCAACTACGACGGCGTCAATAAATCGATCACACTGATCGGTGAT
GGCGCCTTCATTGGCTCACACTCGGCACTGATAGCACCCGTCAGTGTGGGTGCTGGCGCCACACTCGGTGCCGGAACAGT
ACTGACCCATGATGCACCAGCCCATCAACTTACTGTGGCGCGCGCACGTCAAACCACACTGGACGGCTGGCAACGCCCGA
AAAAGAAAACCTAG

Upstream 100 bases:

>100_bases
TGGTACGTTTTACTAAAGTAATGACGCATCATAAGTAGCGCCCCTCCCACTATGGTTGGAGTGCCTAGAATGCAAATCTC
GCTGAAACCGGACGCTACCA

Downstream 100 bases:

>100_bases
ATACACAAGACGTAACATAATGCTCATCCCTCCTTGTAACGCCTTTCATTCCTGCTTTTCCCGATACAAACTCGTGATGG
CGACGTTGATTGGATAATAT

Product: UDP-N-acetylglucosamine pyrophosphorylase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MPLSLPLHIVILAAGEGKRMKSALPKVLHPIAGKPMLAHVITTARALTPDAIHVVYGHAGNQVRAAFADQTDLHWVEQTQ
QLGTGHAVKQTMSAIPNAANVLVLYGDVPLIRAETLQRLPRASTPIAVLVTELANPAGYGHIVRNSEGKVAAIIEDKDAD
EEQRRIHTVNTGILCAESTALRRWLSKLSNTNMQGEYYLTDIFASATADLTPANMIMVTDAQEVEGVNDLWQLTQLERAW
QIRAARALCLQGARVADPARLDQRGTIRIGQNVHIDIDVVLEGEIELGDNVVIGPFVRLKNVKLGPGTKVHAHCDLEGVT
TTGSALIGPFARLRPETMLADGVHIGNFVETKNTSIGADSKANHLTYLGDAQIGTKVNIGAGTITCNYDGVNKSITLIGD
GAFIGSHSALIAPVSVGAGATLGAGTVLTHDAPAHQLTVARARQTTLDGWQRPKKKT

Sequences:

>Translated_457_residues
MPLSLPLHIVILAAGEGKRMKSALPKVLHPIAGKPMLAHVITTARALTPDAIHVVYGHAGNQVRAAFADQTDLHWVEQTQ
QLGTGHAVKQTMSAIPNAANVLVLYGDVPLIRAETLQRLPRASTPIAVLVTELANPAGYGHIVRNSEGKVAAIIEDKDAD
EEQRRIHTVNTGILCAESTALRRWLSKLSNTNMQGEYYLTDIFASATADLTPANMIMVTDAQEVEGVNDLWQLTQLERAW
QIRAARALCLQGARVADPARLDQRGTIRIGQNVHIDIDVVLEGEIELGDNVVIGPFVRLKNVKLGPGTKVHAHCDLEGVT
TTGSALIGPFARLRPETMLADGVHIGNFVETKNTSIGADSKANHLTYLGDAQIGTKVNIGAGTITCNYDGVNKSITLIGD
GAFIGSHSALIAPVSVGAGATLGAGTVLTHDAPAHQLTVARARQTTLDGWQRPKKKT
>Mature_456_residues
PLSLPLHIVILAAGEGKRMKSALPKVLHPIAGKPMLAHVITTARALTPDAIHVVYGHAGNQVRAAFADQTDLHWVEQTQQ
LGTGHAVKQTMSAIPNAANVLVLYGDVPLIRAETLQRLPRASTPIAVLVTELANPAGYGHIVRNSEGKVAAIIEDKDADE
EQRRIHTVNTGILCAESTALRRWLSKLSNTNMQGEYYLTDIFASATADLTPANMIMVTDAQEVEGVNDLWQLTQLERAWQ
IRAARALCLQGARVADPARLDQRGTIRIGQNVHIDIDVVLEGEIELGDNVVIGPFVRLKNVKLGPGTKVHAHCDLEGVTT
TGSALIGPFARLRPETMLADGVHIGNFVETKNTSIGADSKANHLTYLGDAQIGTKVNIGAGTITCNYDGVNKSITLIGDG
AFIGSHSALIAPVSVGAGATLGAGTVLTHDAPAHQLTVARARQTTLDGWQRPKKKT

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Escherichia coli, GI1790168, Length=457, Percent_Identity=49.2341356673961, Blast_Score=431, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMU_XYLF2 (B2I874)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829142.1
- ProteinModelPortal:   B2I874
- SMR:   B2I874
- GeneID:   6202936
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0421
- HOGENOM:   HBG688195
- OMA:   GSKVNHL
- ProtClustDB:   CLSK446185
- GO:   GO:0005737
- HAMAP:   MF_01631
- InterPro:   IPR005882
- InterPro:   IPR005835
- InterPro:   IPR011004
- TIGRFAMs:   TIGR01173

Pfam domain/function: PF00483 NTP_transferase; SSF51161 Trimer_LpxA_like

EC number: =2.7.7.23; =2.3.1.157

Molecular weight: Translated: 48749; Mature: 48618

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: ACT_SITE 364-364 BINDING 78-78 BINDING 140-140 BINDING 155-155 BINDING 170-170 BINDING 388-388 BINDING 406-406 BINDING 424-424 BINDING 441-441

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSLPLHIVILAAGEGKRMKSALPKVLHPIAGKPMLAHVITTARALTPDAIHVVYGHAG
CCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCC
NQVRAAFADQTDLHWVEQTQQLGTGHAVKQTMSAIPNAANVLVLYGDVPLIRAETLQRLP
CEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCEEHHHHHHHCC
RASTPIAVLVTELANPAGYGHIVRNSEGKVAAIIEDKDADEEQRRIHTVNTGILCAESTA
CCCCCHHHHEEHHCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHEEEECCCEEEECHHH
LRRWLSKLSNTNMQGEYYLTDIFASATADLTPANMIMVTDAQEVEGVNDLWQLTQLERAW
HHHHHHHHHCCCCCCCEEEEEEHHHCCCCCCCCCEEEEECHHHHCCHHHHHHHHHHHHHH
QIRAARALCLQGARVADPARLDQRGTIRIGQNVHIDIDVVLEGEIELGDNVVIGPFVRLK
HHHHHHHHHHCCCCCCCCHHCCCCCCEEECCEEEEEEEEEEECCEEECCCEEEECEEEEE
NVKLGPGTKVHAHCDLEGVTTTGSALIGPFARLRPETMLADGVHIGNFVETKNTSIGADS
EEECCCCCEEEEEECCCCCEECCCHHHHHHHHCCCCHHHCCCCEECCEEECCCCCCCCCC
KANHLTYLGDAQIGTKVNIGAGTITCNYDGVNKSITLIGDGAFIGSHSALIAPVSVGAGA
CCCEEEEEECCCCCCEEEECCEEEEEEECCCCCEEEEEECCEEECCCCEEEEEEECCCCC
TLGAGTVLTHDAPAHQLTVARARQTTLDGWQRPKKKT
CCCCCEEEECCCCCHHEEHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PLSLPLHIVILAAGEGKRMKSALPKVLHPIAGKPMLAHVITTARALTPDAIHVVYGHAG
CCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCC
NQVRAAFADQTDLHWVEQTQQLGTGHAVKQTMSAIPNAANVLVLYGDVPLIRAETLQRLP
CEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCEEHHHHHHHCC
RASTPIAVLVTELANPAGYGHIVRNSEGKVAAIIEDKDADEEQRRIHTVNTGILCAESTA
CCCCCHHHHEEHHCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHEEEECCCEEEECHHH
LRRWLSKLSNTNMQGEYYLTDIFASATADLTPANMIMVTDAQEVEGVNDLWQLTQLERAW
HHHHHHHHHCCCCCCCEEEEEEHHHCCCCCCCCCEEEEECHHHHCCHHHHHHHHHHHHHH
QIRAARALCLQGARVADPARLDQRGTIRIGQNVHIDIDVVLEGEIELGDNVVIGPFVRLK
HHHHHHHHHHCCCCCCCCHHCCCCCCEEECCEEEEEEEEEEECCEEECCCEEEECEEEEE
NVKLGPGTKVHAHCDLEGVTTTGSALIGPFARLRPETMLADGVHIGNFVETKNTSIGADS
EEECCCCCEEEEEECCCCCEECCCHHHHHHHHCCCCHHHCCCCEECCEEECCCCCCCCCC
KANHLTYLGDAQIGTKVNIGAGTITCNYDGVNKSITLIGDGAFIGSHSALIAPVSVGAGA
CCCEEEEEECCCCCCEEEECCEEEEEEECCCCCEEEEEECCEEECCCCEEEEEEECCCCC
TLGAGTVLTHDAPAHQLTVARARQTTLDGWQRPKKKT
CCCCCEEEECCCCCHHEEHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA