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The map label for this gene is yafC [C]

Identifier: 182680977

GI number: 182680977

Start: 520151

End: 521056

Strand: Reverse

Name: yafC [C]

Synonym: XfasM23_0415

Alternate gene names: 182680977

Gene position: 521056-520151 (Counterclockwise)

Preceding gene: 182680982

Following gene: 182680975

Centisome position: 20.55

GC content: 55.85

Gene sequence:

>906_bases
ATGCAGAACATGTTCGATGGTGTGCAACTATTCGTTGAAGTCGTTGAAGCCGGCGGCTTCGCCAAAGCAGGTAAACGACT
TTCACTCACCCGCTCCACAATCGGCAAAGCAATCGCCCGTCTAGAAATGCGCCTCGGCGTCCAGCTTTTTCAACGGACAA
CGAGAGTGCAGTCCCTCACCGAAGACGGCCAGCAGTATTACGAACGGTGCCTTCGTGCAATCAAAGAATTACGTGCTGGC
GAAGCCATGCTTGAGCAAGGTCGGCGAGAAGTGGCCGGACGACTGCATGTCTCCTTGCCGGTTCTGTTCGGACGGCAATG
TGTCGCCCCCATCCTGCGCACCTATGCACGAGAACATCCCCAGCTAGAACTCGAACTAAATTTCAGCGACCATCATGTCG
ACCTGATTGCCGACGGTTTCGACCTTGCGGTACGCAACGGCCCTCTGGGCAATGGCACCACACTACGCGCACGCCGCTTA
GGGAACCAACGCAAAATCCTATGCGCTTCACCGACTTACTTGGCCGCAAGAGGCAAACCCCACACACTTGCAGACCTCGC
AGACCACGACTTGCTCACCTACTGCCGCAATGACCGTAACATGGCCTGGACACTTCCCGACGCAACAGGTGCCCTCGTCG
ACATTCCGATCACCTCCCGACTGCGTTGCAACGATCTGGAGGTCATTGCGGATACCGCCGTCGATGGAATGGGAGTTGCC
TGGCTACCCTACTGGCTACTTATGGAACGGATGCAACGCGGCGAACTCATCGGCATTTGGGGGGATCGTCCAAGTGCAGT
AATAGAGTGTCATGTAGTTTGGCCTGCCGCCCAGCATGTGCCCCTACGCTCCAGACTAGCAATCAACCTACTTGCCGCTG
AATTACCTAAATATCTGGAAGCCTGA

Upstream 100 bases:

>100_bases
AAGCAGAATTCATATCAACAATAGAGGATACATTTCCACTCAATGATTCAAAAAATTCTACAATAATAAATTATATAAAT
TTTATGAGGTATATGAAGAT

Downstream 100 bases:

>100_bases
GATGTATAGTGGCATTTGACTAACCCTCATAACAAAAAATAACAAAATTCCCCATAACCCTATCAACGACGTACATCAAC
ATCGCATCAATCGTTACCAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MQNMFDGVQLFVEVVEAGGFAKAGKRLSLTRSTIGKAIARLEMRLGVQLFQRTTRVQSLTEDGQQYYERCLRAIKELRAG
EAMLEQGRREVAGRLHVSLPVLFGRQCVAPILRTYAREHPQLELELNFSDHHVDLIADGFDLAVRNGPLGNGTTLRARRL
GNQRKILCASPTYLAARGKPHTLADLADHDLLTYCRNDRNMAWTLPDATGALVDIPITSRLRCNDLEVIADTAVDGMGVA
WLPYWLLMERMQRGELIGIWGDRPSAVIECHVVWPAAQHVPLRSRLAINLLAAELPKYLEA

Sequences:

>Translated_301_residues
MQNMFDGVQLFVEVVEAGGFAKAGKRLSLTRSTIGKAIARLEMRLGVQLFQRTTRVQSLTEDGQQYYERCLRAIKELRAG
EAMLEQGRREVAGRLHVSLPVLFGRQCVAPILRTYAREHPQLELELNFSDHHVDLIADGFDLAVRNGPLGNGTTLRARRL
GNQRKILCASPTYLAARGKPHTLADLADHDLLTYCRNDRNMAWTLPDATGALVDIPITSRLRCNDLEVIADTAVDGMGVA
WLPYWLLMERMQRGELIGIWGDRPSAVIECHVVWPAAQHVPLRSRLAINLLAAELPKYLEA
>Mature_301_residues
MQNMFDGVQLFVEVVEAGGFAKAGKRLSLTRSTIGKAIARLEMRLGVQLFQRTTRVQSLTEDGQQYYERCLRAIKELRAG
EAMLEQGRREVAGRLHVSLPVLFGRQCVAPILRTYAREHPQLELELNFSDHHVDLIADGFDLAVRNGPLGNGTTLRARRL
GNQRKILCASPTYLAARGKPHTLADLADHDLLTYCRNDRNMAWTLPDATGALVDIPITSRLRCNDLEVIADTAVDGMGVA
WLPYWLLMERMQRGELIGIWGDRPSAVIECHVVWPAAQHVPLRSRLAINLLAAELPKYLEA

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=30.5084745762712, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI145693193, Length=295, Percent_Identity=28.4745762711864, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1789639, Length=286, Percent_Identity=29.3706293706294, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI87081978, Length=257, Percent_Identity=33.0739299610895, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1789440, Length=288, Percent_Identity=26.3888888888889, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787589, Length=271, Percent_Identity=25.4612546125461, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787128, Length=279, Percent_Identity=26.5232974910394, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1789173, Length=271, Percent_Identity=25.830258302583, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788706, Length=270, Percent_Identity=28.1481481481481, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1786448, Length=270, Percent_Identity=30, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082132, Length=265, Percent_Identity=24.5283018867925, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788481, Length=259, Percent_Identity=25.4826254826255, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786713, Length=274, Percent_Identity=26.6423357664234, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787601, Length=211, Percent_Identity=23.696682464455, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI157672245, Length=147, Percent_Identity=25.8503401360544, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33684; Mature: 33684

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNMFDGVQLFVEVVEAGGFAKAGKRLSLTRSTIGKAIARLEMRLGVQLFQRTTRVQSLT
CCCHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDGQQYYERCLRAIKELRAGEAMLEQGRREVAGRLHVSLPVLFGRQCVAPILRTYAREHP
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCEEEEEHHHHHHHHHHHHHHHHHHCCCC
QLELELNFSDHHVDLIADGFDLAVRNGPLGNGTTLRARRLGNQRKILCASPTYLAARGKP
CEEEEECCCCCEEEEEECCEEEEEECCCCCCCCCHHHHHCCCCCEEEEECCCEEEECCCC
HTLADLADHDLLTYCRNDRNMAWTLPDATGALVDIPITSRLRCNDLEVIADTAVDGMGVA
CCHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEECCCCCCCCCCCHHHHHHHCCCCCCHH
WLPYWLLMERMQRGELIGIWGDRPSAVIECHVVWPAAQHVPLRSRLAINLLAAELPKYLE
HHHHHHHHHHHHCCCEEEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHC
A
C
>Mature Secondary Structure
MQNMFDGVQLFVEVVEAGGFAKAGKRLSLTRSTIGKAIARLEMRLGVQLFQRTTRVQSLT
CCCHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDGQQYYERCLRAIKELRAGEAMLEQGRREVAGRLHVSLPVLFGRQCVAPILRTYAREHP
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCEEEEEHHHHHHHHHHHHHHHHHHCCCC
QLELELNFSDHHVDLIADGFDLAVRNGPLGNGTTLRARRLGNQRKILCASPTYLAARGKP
CEEEEECCCCCEEEEEECCEEEEEECCCCCCCCCHHHHHCCCCCEEEEECCCEEEECCCC
HTLADLADHDLLTYCRNDRNMAWTLPDATGALVDIPITSRLRCNDLEVIADTAVDGMGVA
CCHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEECCCCCCCCCCCHHHHHHHCCCCCCHH
WLPYWLLMERMQRGELIGIWGDRPSAVIECHVVWPAAQHVPLRSRLAINLLAAELPKYLE
HHHHHHHHHHHHCCCEEEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]