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The map label for this gene is rpfG [H]

Identifier: 182680961

GI number: 182680961

Start: 495857

End: 497011

Strand: Direct

Name: rpfG [H]

Synonym: XfasM23_0399

Alternate gene names: 182680961

Gene position: 495857-497011 (Clockwise)

Preceding gene: 182680960

Following gene: 182680962

Centisome position: 19.56

GC content: 52.29

Gene sequence:

>1155_bases
ATGCATGATGTTTTTGCTATGCAGTCCGGTGAATTGTCTTCGCTACAGCATTTGGATAGCTGTGACGCGGTGCATGTGGT
ATTGAACATTGTGATTGTCGACGATCAGATGTCGGCTAGGACGATGCTCCGCCATGTGATTGAGGACATTGCGCCTCATT
TGCAAGTTTTTGACTTCGGTGATCCATCAGAAGCGCTGGCTTGGTGTGAGGTTAGCCAAGTTGATTTATTATTGCTTGAT
TACCGGATGCCGGATATGGATGGTCTGGAATTCGCTATGCGCCTCCATCAGCTGCCCAATCGCCGCGATATTCCTATTCT
ATTGATTACCATCGTCGGTGATGAGCCGATCCGTCAGGCTGCGTTGGATGCCGGTGTCATTGATTTTCTGATTAAACCGA
TCCGTCCTCGTGAACTACGCGCCCGTTGCAATAACTTATTGCAGCTACGCCAGCATAGTGAAAGGGCGAAACAACGTGTG
CTGTCCTTGGAGCAGCGGTTGCGCGCTGTGATGAGTGAGTTCGAAGAACGGGAGCGTGAAACCGTCTCCCGTTTAGCTCG
GGCGGTTGAGTACCGCCATATCAGCAGCAGTACCTATCTCGAGCGCATGTCGCAGGTCGCCGGATTAATTGCTGAGCAAC
TTGGTCTGTCTGAGGAAGAGGTGCGACTTATCCAGAGTGCCGCACCGCTTCATGATATTGGCAAGATTGCCATTCCCGAT
GCGGTGTTGCTTAAGCGGAGCACGTTGAACGAGGAGGAGATGGCCATGATGCGTCGGCATCCGCGTATTGGTTGCGAGTT
GCTGAGTGGCAGCCAGAACCGTTGTATCCAGCTGGGTGCATTGATTGCGTTGCGCCATCACGAGCGTTACGACGGCAGTG
GCTATCCAGATGGCTTGGTGGGTGAGGCTATTCCTTTGGAAGCGCGTATTGTTGCTTTGGCTGATGTATTTGACGCATTG
ATCTCATTGCGGCCTTACAAGCAGGCGTGGACTATGGAGGCGGCCATCACCTATTTGTACGCGCAGCGTGGGCGTTTATT
TGATCCACATTGTGTCGACGCTCTCATCAGGGGGCGCGATCAACTACAACGCATTTGCGTTAAGTTCTCCACTGCTTTGG
CCAGGCCGGGAGTGTGGGATGCTAAAGAAAGCTAA

Upstream 100 bases:

>100_bases
GTGAGTCGGATTCAACCTATGCTGCCTGTACATTCCCACTTTTATAGTGGGGCGGGTGACGTACGAAAGTGGGTGGCATA
CTTCCTTGAAGGGGGCGCGT

Downstream 100 bases:

>100_bases
ATGGATATGCAGTCGGCTATCGCAGCGGCCAGATAGTGAGCATGTACAAAATTTAATCAGATTTCTTATCGCCTCGCTCT
TCATCATCTACTTGAGCGTA

Product: response regulator receiver modulated metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MHDVFAMQSGELSSLQHLDSCDAVHVVLNIVIVDDQMSARTMLRHVIEDIAPHLQVFDFGDPSEALAWCEVSQVDLLLLD
YRMPDMDGLEFAMRLHQLPNRRDIPILLITIVGDEPIRQAALDAGVIDFLIKPIRPRELRARCNNLLQLRQHSERAKQRV
LSLEQRLRAVMSEFEERERETVSRLARAVEYRHISSSTYLERMSQVAGLIAEQLGLSEEEVRLIQSAAPLHDIGKIAIPD
AVLLKRSTLNEEEMAMMRRHPRIGCELLSGSQNRCIQLGALIALRHHERYDGSGYPDGLVGEAIPLEARIVALADVFDAL
ISLRPYKQAWTMEAAITYLYAQRGRLFDPHCVDALIRGRDQLQRICVKFSTALARPGVWDAKES

Sequences:

>Translated_384_residues
MHDVFAMQSGELSSLQHLDSCDAVHVVLNIVIVDDQMSARTMLRHVIEDIAPHLQVFDFGDPSEALAWCEVSQVDLLLLD
YRMPDMDGLEFAMRLHQLPNRRDIPILLITIVGDEPIRQAALDAGVIDFLIKPIRPRELRARCNNLLQLRQHSERAKQRV
LSLEQRLRAVMSEFEERERETVSRLARAVEYRHISSSTYLERMSQVAGLIAEQLGLSEEEVRLIQSAAPLHDIGKIAIPD
AVLLKRSTLNEEEMAMMRRHPRIGCELLSGSQNRCIQLGALIALRHHERYDGSGYPDGLVGEAIPLEARIVALADVFDAL
ISLRPYKQAWTMEAAITYLYAQRGRLFDPHCVDALIRGRDQLQRICVKFSTALARPGVWDAKES
>Mature_384_residues
MHDVFAMQSGELSSLQHLDSCDAVHVVLNIVIVDDQMSARTMLRHVIEDIAPHLQVFDFGDPSEALAWCEVSQVDLLLLD
YRMPDMDGLEFAMRLHQLPNRRDIPILLITIVGDEPIRQAALDAGVIDFLIKPIRPRELRARCNNLLQLRQHSERAKQRV
LSLEQRLRAVMSEFEERERETVSRLARAVEYRHISSSTYLERMSQVAGLIAEQLGLSEEEVRLIQSAAPLHDIGKIAIPD
AVLLKRSTLNEEEMAMMRRHPRIGCELLSGSQNRCIQLGALIALRHHERYDGSGYPDGLVGEAIPLEARIVALADVFDAL
ISLRPYKQAWTMEAAITYLYAQRGRLFDPHCVDALIRGRDQLQRICVKFSTALARPGVWDAKES

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=173, Percent_Identity=24.2774566473988, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789149, Length=147, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1786911, Length=125, Percent_Identity=34.4, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786599, Length=135, Percent_Identity=30.3703703703704, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 43535; Mature: 43535

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDVFAMQSGELSSLQHLDSCDAVHVVLNIVIVDDQMSARTMLRHVIEDIAPHLQVFDFG
CCCCCCCCCCCHHHHHHHCCCHHHHHHHHHEEECCCHHHHHHHHHHHHHHHCCCEEEECC
DPSEALAWCEVSQVDLLLLDYRMPDMDGLEFAMRLHQLPNRRDIPILLITIVGDEPIRQA
CHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHH
ALDAGVIDFLIKPIRPRELRARCNNLLQLRQHSERAKQRVLSLEQRLRAVMSEFEERERE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVSRLARAVEYRHISSSTYLERMSQVAGLIAEQLGLSEEEVRLIQSAAPLHDIGKIAIPD
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHCCCCCCCH
AVLLKRSTLNEEEMAMMRRHPRIGCELLSGSQNRCIQLGALIALRHHERYDGSGYPDGLV
HHHHHHHCCCHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
GEAIPLEARIVALADVFDALISLRPYKQAWTMEAAITYLYAQRGRLFDPHCVDALIRGRD
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHH
QLQRICVKFSTALARPGVWDAKES
HHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MHDVFAMQSGELSSLQHLDSCDAVHVVLNIVIVDDQMSARTMLRHVIEDIAPHLQVFDFG
CCCCCCCCCCCHHHHHHHCCCHHHHHHHHHEEECCCHHHHHHHHHHHHHHHCCCEEEECC
DPSEALAWCEVSQVDLLLLDYRMPDMDGLEFAMRLHQLPNRRDIPILLITIVGDEPIRQA
CHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHH
ALDAGVIDFLIKPIRPRELRARCNNLLQLRQHSERAKQRVLSLEQRLRAVMSEFEERERE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVSRLARAVEYRHISSSTYLERMSQVAGLIAEQLGLSEEEVRLIQSAAPLHDIGKIAIPD
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHCCCCCCCH
AVLLKRSTLNEEEMAMMRRHPRIGCELLSGSQNRCIQLGALIALRHHERYDGSGYPDGLV
HHHHHHHCCCHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
GEAIPLEARIVALADVFDALISLRPYKQAWTMEAAITYLYAQRGRLFDPHCVDALIRGRD
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHH
QLQRICVKFSTALARPGVWDAKES
HHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]