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The map label for this gene is lysS

Identifier: 182680960

GI number: 182680960

Start: 494199

End: 495719

Strand: Direct

Name: lysS

Synonym: XfasM23_0398

Alternate gene names: 182680960

Gene position: 494199-495719 (Clockwise)

Preceding gene: 182680959

Following gene: 182680961

Centisome position: 19.49

GC content: 52.86

Gene sequence:

>1521_bases
ATGACTGAGCATCTCCCTGCGTCGCAGGTGTCCTTTGACGAGAACGCGCTGATTGCCGAGCGCCGTGCAAAACTTTTAGC
ACTACGTGCGCAAGGCGTGGCTTATCCGAATGATGTCAAACGCGAGCATTATGCTGCTGATGTGCAGGCAGCATTTGCCA
ACGTCGAGACATGGACTGCTGAGACCCTGGAGGCGTCAAGCCATCGTGTACGTATGGCTGGCCGATTGATGGCTAAGCGT
CTCATGGGCAAGGCCAGCTTTGCTCAGATTCAGGACGAGTCTGGCCGCATCCAGCTGTTAATACAGAGTAACGTGCTTGG
TGAGGACAGCTATGCCGCATTCAAGGTGTTGGATGTTGGCGACATCATTGCTGTTGAAGGAGGATTGACCCGCACCAGAA
CGGGCGAGCTGTCGGTCAAGGTGAATGTTTTGCGACTTTTGACGAAGGCGTTACGTCCATTGCCTGACAAGTGGCACGGG
CTGACTGATGTGGAGCAGCGCTACCGCCAGCGCTATGTTGATCTTATTGTGACTCCAGAATCGCGTGAGATCTTCATCAA
ACGCTCCAAGATCATTCGTGCGCTGCGTACTTGGCTGGATGCGCGTCTTTTCTTGGAAGTAGAGACGCCGATGATGCATT
ACATCCCAGGAGGTGCTGCGGCTAAGCCGTTTGTGACCTACCACAATGCCTTGGATTTAGAGTTATATCTGCGCGTGGCC
CCAGAGTTGTATCTCAAGCGTTTGGTCGTGGGTGGGCTGGAACGGGTTTACGAGATTAACCGCAACTTCCGTAACGAGGG
GGTGTCGACCCGGCACAACCCCGAATTTACGATGCTTGAGTTATACGAGGCCTATTCCACCTACCATGAGGTCATGGATC
TGGCTGAGACGATGATCCGTGATACTGCACAGTCTGTGCTGGGCACGACCCAGGTGATTTGGGACGGTGCTCAGATTGAT
CTGGGTCCGATATTCCGGCGTTGGCGCATGGATGAGGCAGTTTGCCATCATAATCCTGAGATGAGCGTTGCCGAGTGTAC
CGATCGTGATGCATTGCTCCTTCACTGTGAGAGACTCAAGATCAAGGTTAAATCGTCCTACGGTTGGGGTCGATTGCTCT
TGAGTATTTTTGAGGCGACTGTTGAGCATACGTTGATCCAGCCGACCTTCATTACGGATCATCCGGTTGAAATCTCTCCT
TTAGCGCGCGAAAGCGACATCGAATCAGGGTATACCGATCGCTTCGAACTGTTCATCAATGGGAAGGAAATCGCCAACGG
TTTCTCGGAATTGAACGATCCGGAGGAGCAGGCGATGCGCTTCCAAAAGCAGGTTGAAGCCAAAGAAGGGGGGGATGATG
AGGCCATGTACTACGATGCCGACTATATTCGCGCTTTGGAGTATGGGATGGCGCCAACCGGAGGGTTAGGGATTGGTGTT
GATCGACTGGTGATGCTGCTGACCGGTAGCACCTCAATCCGCGATGTGCTGTTATTCCCTTATATGCGTCCAGAACGTTA
A

Upstream 100 bases:

>100_bases
GACTGCGTTATCCGATTTAAAAAAGTATCCGAGTTTCTTTGGTGTCACCCTTATTCCCGAATCTGGAACGTCCTGTGTCC
TTTAACGATAGATCATCTTC

Downstream 100 bases:

>100_bases
TTGATATCCAATCAAAATGTGACGGGAAGCAATTATCGTGAGTCGGATTCAACCTATGCTGCCTGTACATTCCCACTTTT
ATAGTGGGGCGGGTGACGTA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MTEHLPASQVSFDENALIAERRAKLLALRAQGVAYPNDVKREHYAADVQAAFANVETWTAETLEASSHRVRMAGRLMAKR
LMGKASFAQIQDESGRIQLLIQSNVLGEDSYAAFKVLDVGDIIAVEGGLTRTRTGELSVKVNVLRLLTKALRPLPDKWHG
LTDVEQRYRQRYVDLIVTPESREIFIKRSKIIRALRTWLDARLFLEVETPMMHYIPGGAAAKPFVTYHNALDLELYLRVA
PELYLKRLVVGGLERVYEINRNFRNEGVSTRHNPEFTMLELYEAYSTYHEVMDLAETMIRDTAQSVLGTTQVIWDGAQID
LGPIFRRWRMDEAVCHHNPEMSVAECTDRDALLLHCERLKIKVKSSYGWGRLLLSIFEATVEHTLIQPTFITDHPVEISP
LARESDIESGYTDRFELFINGKEIANGFSELNDPEEQAMRFQKQVEAKEGGDDEAMYYDADYIRALEYGMAPTGGLGIGV
DRLVMLLTGSTSIRDVLLFPYMRPER

Sequences:

>Translated_506_residues
MTEHLPASQVSFDENALIAERRAKLLALRAQGVAYPNDVKREHYAADVQAAFANVETWTAETLEASSHRVRMAGRLMAKR
LMGKASFAQIQDESGRIQLLIQSNVLGEDSYAAFKVLDVGDIIAVEGGLTRTRTGELSVKVNVLRLLTKALRPLPDKWHG
LTDVEQRYRQRYVDLIVTPESREIFIKRSKIIRALRTWLDARLFLEVETPMMHYIPGGAAAKPFVTYHNALDLELYLRVA
PELYLKRLVVGGLERVYEINRNFRNEGVSTRHNPEFTMLELYEAYSTYHEVMDLAETMIRDTAQSVLGTTQVIWDGAQID
LGPIFRRWRMDEAVCHHNPEMSVAECTDRDALLLHCERLKIKVKSSYGWGRLLLSIFEATVEHTLIQPTFITDHPVEISP
LARESDIESGYTDRFELFINGKEIANGFSELNDPEEQAMRFQKQVEAKEGGDDEAMYYDADYIRALEYGMAPTGGLGIGV
DRLVMLLTGSTSIRDVLLFPYMRPER
>Mature_505_residues
TEHLPASQVSFDENALIAERRAKLLALRAQGVAYPNDVKREHYAADVQAAFANVETWTAETLEASSHRVRMAGRLMAKRL
MGKASFAQIQDESGRIQLLIQSNVLGEDSYAAFKVLDVGDIIAVEGGLTRTRTGELSVKVNVLRLLTKALRPLPDKWHGL
TDVEQRYRQRYVDLIVTPESREIFIKRSKIIRALRTWLDARLFLEVETPMMHYIPGGAAAKPFVTYHNALDLELYLRVAP
ELYLKRLVVGGLERVYEINRNFRNEGVSTRHNPEFTMLELYEAYSTYHEVMDLAETMIRDTAQSVLGTTQVIWDGAQIDL
GPIFRRWRMDEAVCHHNPEMSVAECTDRDALLLHCERLKIKVKSSYGWGRLLLSIFEATVEHTLIQPTFITDHPVEISPL
ARESDIESGYTDRFELFINGKEIANGFSELNDPEEQAMRFQKQVEAKEGGDDEAMYYDADYIRALEYGMAPTGGLGIGVD
RLVMLLTGSTSIRDVLLFPYMRPER

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI5031815, Length=451, Percent_Identity=45.2328159645233, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI194272210, Length=451, Percent_Identity=45.2328159645233, Blast_Score=378, Evalue=1e-105,
Organism=Homo sapiens, GI45439306, Length=352, Percent_Identity=26.1363636363636, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1790571, Length=497, Percent_Identity=56.1368209255533, Blast_Score=576, Evalue=1e-165,
Organism=Escherichia coli, GI1789256, Length=503, Percent_Identity=54.8707753479125, Blast_Score=566, Evalue=1e-163,
Organism=Escherichia coli, GI87082379, Length=328, Percent_Identity=31.7073170731707, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1788173, Length=248, Percent_Identity=27.4193548387097, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=452, Percent_Identity=43.141592920354, Blast_Score=350, Evalue=7e-97,
Organism=Caenorhabditis elegans, GI17535927, Length=452, Percent_Identity=43.141592920354, Blast_Score=350, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI71994340, Length=452, Percent_Identity=43.141592920354, Blast_Score=349, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI17551876, Length=356, Percent_Identity=28.3707865168539, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI32566633, Length=256, Percent_Identity=27.34375, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=494, Percent_Identity=40.8906882591093, Blast_Score=350, Evalue=3e-97,
Organism=Saccharomyces cerevisiae, GI6324256, Length=479, Percent_Identity=31.7327766179541, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6323011, Length=352, Percent_Identity=26.1363636363636, Blast_Score=87, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24640849, Length=488, Percent_Identity=40.9836065573771, Blast_Score=359, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24640851, Length=488, Percent_Identity=40.9836065573771, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI24584738, Length=299, Percent_Identity=26.4214046822742, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYK_XYLF2 (B2I848)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829120.1
- ProteinModelPortal:   B2I848
- SMR:   B2I848
- GeneID:   6202710
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0398
- HOGENOM:   HBG631383
- OMA:   VDMIANP
- ProtClustDB:   PRK00484
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 57565; Mature: 57433

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHLPASQVSFDENALIAERRAKLLALRAQGVAYPNDVKREHYAADVQAAFANVETWTA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHH
ETLEASSHRVRMAGRLMAKRLMGKASFAQIQDESGRIQLLIQSNVLGEDSYAAFKVLDVG
HHHHCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEECC
DIIAVEGGLTRTRTGELSVKVNVLRLLTKALRPLPDKWHGLTDVEQRYRQRYVDLIVTPE
CEEEECCCCCCCCCCCEEEEHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHEEEEECCC
SREIFIKRSKIIRALRTWLDARLFLEVETPMMHYIPGGAAAKPFVTYHNALDLELYLRVA
CCEEEHHHHHHHHHHHHHHCCEEEEEECCCEEEECCCCCCCCCEEEECCCCCEEHHHHHH
PELYLKRLVVGGLERVYEINRNFRNEGVSTRHNPEFTMLELYEAYSTYHEVMDLAETMIR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DTAQSVLGTTQVIWDGAQIDLGPIFRRWRMDEAVCHHNPEMSVAECTDRDALLLHCERLK
HHHHHHHCCHHEEECCCEECHHHHHHHHCCHHHHHHCCCCCCHHHCCCCCCEEEEEEEEE
IKVKSSYGWGRLLLSIFEATVEHTLIQPTFITDHPVEISPLARESDIESGYTDRFELFIN
EEEECCCCHHHHHHHHHHHHHHHHEECCEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEC
GKEIANGFSELNDPEEQAMRFQKQVEAKEGGDDEAMYYDADYIRALEYGMAPTGGLGIGV
CHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHCCCCCCCCCCCH
DRLVMLLTGSTSIRDVLLFPYMRPER
HHHHHHHCCCCCHHHHHEECCCCCCC
>Mature Secondary Structure 
TEHLPASQVSFDENALIAERRAKLLALRAQGVAYPNDVKREHYAADVQAAFANVETWTA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHH
ETLEASSHRVRMAGRLMAKRLMGKASFAQIQDESGRIQLLIQSNVLGEDSYAAFKVLDVG
HHHHCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEECC
DIIAVEGGLTRTRTGELSVKVNVLRLLTKALRPLPDKWHGLTDVEQRYRQRYVDLIVTPE
CEEEECCCCCCCCCCCEEEEHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHEEEEECCC
SREIFIKRSKIIRALRTWLDARLFLEVETPMMHYIPGGAAAKPFVTYHNALDLELYLRVA
CCEEEHHHHHHHHHHHHHHCCEEEEEECCCEEEECCCCCCCCCEEEECCCCCEEHHHHHH
PELYLKRLVVGGLERVYEINRNFRNEGVSTRHNPEFTMLELYEAYSTYHEVMDLAETMIR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DTAQSVLGTTQVIWDGAQIDLGPIFRRWRMDEAVCHHNPEMSVAECTDRDALLLHCERLK
HHHHHHHCCHHEEECCCEECHHHHHHHHCCHHHHHHCCCCCCHHHCCCCCCEEEEEEEEE
IKVKSSYGWGRLLLSIFEATVEHTLIQPTFITDHPVEISPLARESDIESGYTDRFELFIN
EEEECCCCHHHHHHHHHHHHHHHHEECCEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEC
GKEIANGFSELNDPEEQAMRFQKQVEAKEGGDDEAMYYDADYIRALEYGMAPTGGLGIGV
CHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHCCCCCCCCCCCH
DRLVMLLTGSTSIRDVLLFPYMRPER
HHHHHHHCCCCCHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA