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The map label for this gene is prfB [H]

Identifier: 182680959

GI number: 182680959

Start: 493109

End: 494017

Strand: Direct

Name: prfB [H]

Synonym: XfasM23_0397

Alternate gene names: 182680959

Gene position: 493109-494017 (Clockwise)

Preceding gene: 182680958

Following gene: 182680960

Centisome position: 19.45

GC content: 53.03

Gene sequence:

>909_bases
GTGCTGAACGGGGTCGCTGAAGCCAGCGAATTTTTGGATCTGGCTGAAATAGAGCAAGATCAGGAGATGTTGCAGGCTGT
CCTGAACGACGTGGACATCTATCAACTTCAGGTCGAGAAACTGGAGTTTCAGCGCATGTTTTCCGGGAAGATGGACGGTG
CCAATGCGTTTGTTGACATCCAGGCGGGTGCTGGAGGGACCGAGGCCCAAGATTGGGCAGAGATGCTGCTGCGTATGTAT
TTGCGTTGGGCCGAGGGGCGTGGTTGGAAGGCCGAATTGCTTGAAGTTTCTGGCGGTGAAGTGGCCGGCATCAAATCAGC
CACGTTTCGCGTGGAGGGCGATTTTGCCTACGGTTGGTTGAAGACGGAGACTGGGGTGCATCGTTTGGTGCGTAAGTCGC
CATTTGATTCGGATAACCGTCGCCATACGAGTTTTACCTCTGTCTTTGTTTCCCCGGAGGTCGATGACAACATCGACATT
GAGATTAACCCGGCCGACTTGAAGACCGATGTATACCGCTCCTCTGGGGCTGGTGGTCAGCACGTGAACAAGACTGAGTC
TGCAGTACGTATCACCCACGTACCGAGTGGGATTGTGGTGGCCTGCCAGACGGAACGTAGTCAGCATGCTAACCGTGATC
GTGCGATGAAGATGCTGGCGGCCAAGTTGTACGAGTCGGAGATCCAGAAGCGGAATGCCGAAAAAGACGTACTTGAGGCC
TCTAAGTCTGATATCGGCTGGGGGAGTCAGATTCGCAATTACGTATTGGATCAGAGTCGGATTAAAGATCTTCGTACTGG
CGTTGAGCGCAGCGATACTCAGAAAGTACTTGACGGCGATTTAGATGAGTTTATTGAAGCGAGTTTGAAGGCTGGTTTGG
AAGCAGGCGCTAAACGCGCCGATGCATGA

Upstream 100 bases:

>100_bases
TGAGCTGGAAAGCCCCACGATCTGGAATACTCCAGACTATGCGCAGCAACTGGGCCGGGAACGTTCGCTACTGGATAAGA
CCGTCACCGGCATTGCTCAT

Downstream 100 bases:

>100_bases
TTCACCTATAGTTTCGGGTGCCGCTACCCTACGGTATCACTTTTGAAGTGTATACGGCATCCAGTATGACGACGTTGCTT
TGACTGCGTTATCCGATTTA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MLNGVAEASEFLDLAEIEQDQEMLQAVLNDVDIYQLQVEKLEFQRMFSGKMDGANAFVDIQAGAGGTEAQDWAEMLLRMY
LRWAEGRGWKAELLEVSGGEVAGIKSATFRVEGDFAYGWLKTETGVHRLVRKSPFDSDNRRHTSFTSVFVSPEVDDNIDI
EINPADLKTDVYRSSGAGGQHVNKTESAVRITHVPSGIVVACQTERSQHANRDRAMKMLAAKLYESEIQKRNAEKDVLEA
SKSDIGWGSQIRNYVLDQSRIKDLRTGVERSDTQKVLDGDLDEFIEASLKAGLEAGAKRADA

Sequences:

>Translated_302_residues
MLNGVAEASEFLDLAEIEQDQEMLQAVLNDVDIYQLQVEKLEFQRMFSGKMDGANAFVDIQAGAGGTEAQDWAEMLLRMY
LRWAEGRGWKAELLEVSGGEVAGIKSATFRVEGDFAYGWLKTETGVHRLVRKSPFDSDNRRHTSFTSVFVSPEVDDNIDI
EINPADLKTDVYRSSGAGGQHVNKTESAVRITHVPSGIVVACQTERSQHANRDRAMKMLAAKLYESEIQKRNAEKDVLEA
SKSDIGWGSQIRNYVLDQSRIKDLRTGVERSDTQKVLDGDLDEFIEASLKAGLEAGAKRADA
>Mature_302_residues
MLNGVAEASEFLDLAEIEQDQEMLQAVLNDVDIYQLQVEKLEFQRMFSGKMDGANAFVDIQAGAGGTEAQDWAEMLLRMY
LRWAEGRGWKAELLEVSGGEVAGIKSATFRVEGDFAYGWLKTETGVHRLVRKSPFDSDNRRHTSFTSVFVSPEVDDNIDI
EINPADLKTDVYRSSGAGGQHVNKTESAVRITHVPSGIVVACQTERSQHANRDRAMKMLAAKLYESEIQKRNAEKDVLEA
SKSDIGWGSQIRNYVLDQSRIKDLRTGVERSDTQKVLDGDLDEFIEASLKAGLEAGAKRADA

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=269, Percent_Identity=37.1747211895911, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI34577120, Length=315, Percent_Identity=33.968253968254, Blast_Score=162, Evalue=2e-40,
Organism=Homo sapiens, GI166795305, Length=183, Percent_Identity=34.4262295081967, Blast_Score=115, Evalue=4e-26,
Organism=Escherichia coli, GI2367172, Length=293, Percent_Identity=67.9180887372014, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI1787462, Length=299, Percent_Identity=39.1304347826087, Blast_Score=202, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17542784, Length=315, Percent_Identity=31.7460317460317, Blast_Score=120, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6321295, Length=242, Percent_Identity=38.8429752066116, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI19921226, Length=207, Percent_Identity=41.0628019323672, Blast_Score=154, Evalue=8e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 33551; Mature: 33551

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNGVAEASEFLDLAEIEQDQEMLQAVLNDVDIYQLQVEKLEFQRMFSGKMDGANAFVDI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
QAGAGGTEAQDWAEMLLRMYLRWAEGRGWKAELLEVSGGEVAGIKSATFRVEGDFAYGWL
ECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEECCCEEEEEEECCEEEEEE
KTETGVHRLVRKSPFDSDNRRHTSFTSVFVSPEVDDNIDIEINPADLKTDVYRSSGAGGQ
ECHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCHHHHHHHHCCCCCCC
HVNKTESAVRITHVPSGIVVACQTERSQHANRDRAMKMLAAKLYESEIQKRNAEKDVLEA
CCCCCCCEEEEEECCCCEEEEEECCHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
SKSDIGWGSQIRNYVLDQSRIKDLRTGVERSDTQKVLDGDLDEFIEASLKAGLEAGAKRA
CCCCCCCHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCHHCC
DA
CC
>Mature Secondary Structure
MLNGVAEASEFLDLAEIEQDQEMLQAVLNDVDIYQLQVEKLEFQRMFSGKMDGANAFVDI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
QAGAGGTEAQDWAEMLLRMYLRWAEGRGWKAELLEVSGGEVAGIKSATFRVEGDFAYGWL
ECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEECCCEEEEEEECCEEEEEE
KTETGVHRLVRKSPFDSDNRRHTSFTSVFVSPEVDDNIDIEINPADLKTDVYRSSGAGGQ
ECHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCHHHHHHHHCCCCCCC
HVNKTESAVRITHVPSGIVVACQTERSQHANRDRAMKMLAAKLYESEIQKRNAEKDVLEA
CCCCCCCEEEEEECCCCEEEEEECCHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
SKSDIGWGSQIRNYVLDQSRIKDLRTGVERSDTQKVLDGDLDEFIEASLKAGLEAGAKRA
CCCCCCCHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCHHCC
DA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA