Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 182680837

GI number: 182680837

Start: 355852

End: 357516

Strand: Direct

Name: pleD [H]

Synonym: XfasM23_0272

Alternate gene names: 182680837

Gene position: 355852-357516 (Clockwise)

Preceding gene: 182680836

Following gene: 182680841

Centisome position: 14.03

GC content: 54.47

Gene sequence:

>1665_bases
ATGAAGCGTCAGCTTGGTTATTTTCTGTTGAGAGGTGTGTTGTTGTGGCTGTGCTTGGCAGCACCAGCAGTTGCACAGTC
GCTGATGCTGGAACGCTTGGATGACGACCCGCCAGCACACGAGGTGTTGGCGGGTGTATACGATGCTATGTTGCGCCCAA
ATGACACAGGGGGTGCCAGTATCTATGAAACGGCGCGACATCCGGTGTGGTGGCGTATCCGCGCTGACCGCCAGATCAGT
GCAGCTGGGCAACCAAAGCTGCAGATAGAGTTCCCGTATCTGAACTGGGTTGAGGCTTGGGTCCCTGGGCGTAGCGTGCC
GAGCCACCATGCGATCTACGGAGCTGCGGCGGACCGCCGCTATGCAACGCGTGCATTGGTGATCGATTTACCCGAGGGGT
TGCCGCAGGGGAGGGCAGTATGGTTGCGTGTGCATGCGCAGAGCACGATATCAATGCCAGTTTCCATTGTGAGCAATGAT
GTGGTGCATCGTCGTGATCTGGATCATGTTGCTTGGTATAGCTTTATCCTCGGTTTAACGACGGTGCTGGCTGTACTTTC
AGCCTCACTCTGGGTGCGGTTGCGTGAGCCTGTGTATGGCTATTTTACGGTCAATTTGGTGTGTGTACTGCTGTACCTGA
TCGGCTTTGATGGCGAGGCGAGAGGTTTTCCGGTGCTCGGAGAATTGTTTGGCTCCTCACCAGTACCGATACGGGTGGTT
GCCACCTTTGGTGGCTTTGTCATCGTGAATTTCCAGCGTCACTACTTGAACACCGCGTTCCGGATACCACGGGTGGATTT
GGCGTTGAAAGCTTCTTCGGGTTGGTTACTGGTGATGGCAGTGTTGAACGTGGTGCCGCAGATCCAGCCATATCCGGTAC
TCACGTATCTTTCTCTGATGGTTGCTTCGTTGTTGTTGTTGTTTAGTTGTGTATGGTTGGGAGGCGTCCGTGGTCATCGC
GATGCTTGGTCGTTGTTGGTTGCTTGGACCCCTTTGTTATTGTTCTCTATGCTGCGCAGCTTGGAGTTGATTGGCTGGAA
TCGGTTGAGTGGCAATGGCGCTTGGATTGGATATGGGCTGAGCATGTCGCTGGGATTATCTTGTTTGCTGGTGACTCTGG
TACTGGCTGATCATATGCTGGAGTTGCGACGTGATCGCGACCGTGCCAGGTTGCTTGCAAATGTGGATGGTCTGACCGGT
GCGTTGACCCGGGTCGCGATTGAGCGGAGATTGGAGCGCGATATTGATGATGCCCGTCAGATGGGGACTCCTTTATCGGT
GGCGTTTATCGACATTGATCATTTCAAACGTATCAATGACGAATATGGGCATGCTGCTGGAGATGCTTGTTTGCGTCAGG
TGGTGCAGCGGGTGCGCGGTTGGCTACGTGCCAGTGACGCGCTTGGGCGTCATGGAGGTGATGAGTTAATTGCGATCCTT
CCTGGGGCCGACTTGGTAGCAGCGCGGCGTGTCGCCGAGGGGATCCGCGTTGCTGTTTCAAGCCAGCCGTTCCAATTTGA
ATGTACCCGGTTACGTTGCACATTGAGTCTTGGGGTGGCCTCGTGGTTGCCTGATGAATCCATCGAAGCACTATTGCAAC
GTGCCGATAACGGGTTGTATGCAAGCAAGGCGGCTGGACGTGATCGGGTCAGTACTGAGATGTAA

Upstream 100 bases:

>100_bases
GGCAAAGAGAGATTGCAGGGTGGGCGATGCACTTTGGTTTATGGGACCTTCCAGGTCAGTCACTTTCTCAGTGATGCCGT
TGTTGGGAGCTGCTAAGAGA

Downstream 100 bases:

>100_bases
AAAGGAAAAGGGGCATCCATTGTGTCGGTGCCCCGTTCGATACATTGCCAGATTGAGTGAATGTGCTGTTGTGGAGTGGT
TTTGTCAGGTCGCGAATGTC

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MKRQLGYFLLRGVLLWLCLAAPAVAQSLMLERLDDDPPAHEVLAGVYDAMLRPNDTGGASIYETARHPVWWRIRADRQIS
AAGQPKLQIEFPYLNWVEAWVPGRSVPSHHAIYGAAADRRYATRALVIDLPEGLPQGRAVWLRVHAQSTISMPVSIVSND
VVHRRDLDHVAWYSFILGLTTVLAVLSASLWVRLREPVYGYFTVNLVCVLLYLIGFDGEARGFPVLGELFGSSPVPIRVV
ATFGGFVIVNFQRHYLNTAFRIPRVDLALKASSGWLLVMAVLNVVPQIQPYPVLTYLSLMVASLLLLFSCVWLGGVRGHR
DAWSLLVAWTPLLLFSMLRSLELIGWNRLSGNGAWIGYGLSMSLGLSCLLVTLVLADHMLELRRDRDRARLLANVDGLTG
ALTRVAIERRLERDIDDARQMGTPLSVAFIDIDHFKRINDEYGHAAGDACLRQVVQRVRGWLRASDALGRHGGDELIAIL
PGADLVAARRVAEGIRVAVSSQPFQFECTRLRCTLSLGVASWLPDESIEALLQRADNGLYASKAAGRDRVSTEM

Sequences:

>Translated_554_residues
MKRQLGYFLLRGVLLWLCLAAPAVAQSLMLERLDDDPPAHEVLAGVYDAMLRPNDTGGASIYETARHPVWWRIRADRQIS
AAGQPKLQIEFPYLNWVEAWVPGRSVPSHHAIYGAAADRRYATRALVIDLPEGLPQGRAVWLRVHAQSTISMPVSIVSND
VVHRRDLDHVAWYSFILGLTTVLAVLSASLWVRLREPVYGYFTVNLVCVLLYLIGFDGEARGFPVLGELFGSSPVPIRVV
ATFGGFVIVNFQRHYLNTAFRIPRVDLALKASSGWLLVMAVLNVVPQIQPYPVLTYLSLMVASLLLLFSCVWLGGVRGHR
DAWSLLVAWTPLLLFSMLRSLELIGWNRLSGNGAWIGYGLSMSLGLSCLLVTLVLADHMLELRRDRDRARLLANVDGLTG
ALTRVAIERRLERDIDDARQMGTPLSVAFIDIDHFKRINDEYGHAAGDACLRQVVQRVRGWLRASDALGRHGGDELIAIL
PGADLVAARRVAEGIRVAVSSQPFQFECTRLRCTLSLGVASWLPDESIEALLQRADNGLYASKAAGRDRVSTEM
>Mature_554_residues
MKRQLGYFLLRGVLLWLCLAAPAVAQSLMLERLDDDPPAHEVLAGVYDAMLRPNDTGGASIYETARHPVWWRIRADRQIS
AAGQPKLQIEFPYLNWVEAWVPGRSVPSHHAIYGAAADRRYATRALVIDLPEGLPQGRAVWLRVHAQSTISMPVSIVSND
VVHRRDLDHVAWYSFILGLTTVLAVLSASLWVRLREPVYGYFTVNLVCVLLYLIGFDGEARGFPVLGELFGSSPVPIRVV
ATFGGFVIVNFQRHYLNTAFRIPRVDLALKASSGWLLVMAVLNVVPQIQPYPVLTYLSLMVASLLLLFSCVWLGGVRGHR
DAWSLLVAWTPLLLFSMLRSLELIGWNRLSGNGAWIGYGLSMSLGLSCLLVTLVLADHMLELRRDRDRARLLANVDGLTG
ALTRVAIERRLERDIDDARQMGTPLSVAFIDIDHFKRINDEYGHAAGDACLRQVVQRVRGWLRASDALGRHGGDELIAIL
PGADLVAARRVAEGIRVAVSSQPFQFECTRLRCTLSLGVASWLPDESIEALLQRADNGLYASKAAGRDRVSTEM

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=262, Percent_Identity=31.6793893129771, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI145693134, Length=165, Percent_Identity=40, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI87082007, Length=130, Percent_Identity=44.6153846153846, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1788381, Length=161, Percent_Identity=36.6459627329193, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87081881, Length=179, Percent_Identity=34.0782122905028, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787802, Length=167, Percent_Identity=34.1317365269461, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787262, Length=266, Percent_Identity=27.8195488721804, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787816, Length=157, Percent_Identity=30.5732484076433, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1787541, Length=175, Percent_Identity=30.8571428571429, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI87081977, Length=106, Percent_Identity=37.7358490566038, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 61468; Mature: 61468

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRQLGYFLLRGVLLWLCLAAPAVAQSLMLERLDDDPPAHEVLAGVYDAMLRPNDTGGAS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCH
IYETARHPVWWRIRADRQISAAGQPKLQIEFPYLNWVEAWVPGRSVPSHHAIYGAAADRR
HHHHCCCCEEEEEECCCEECCCCCCCEEEECCCHHHHHHHCCCCCCCCCCEEEECCCCCC
YATRALVIDLPEGLPQGRAVWLRVHAQSTISMPVSIVSNDVVHRRDLDHVAWYSFILGLT
HHEEEEEEECCCCCCCCCEEEEEEECCCCCCCCHHHHHCCHHHHCCCHHHHHHHHHHHHH
TVLAVLSASLWVRLREPVYGYFTVNLVCVLLYLIGFDGEARGFPVLGELFGSSPVPIRVV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCEEEE
ATFGGFVIVNFQRHYLNTAFRIPRVDLALKASSGWLLVMAVLNVVPQIQPYPVLTYLSLM
EECCCEEEEEEHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHH
VASLLLLFSCVWLGGVRGHRDAWSLLVAWTPLLLFSMLRSLELIGWNRLSGNGAWIGYGL
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEECC
SMSLGLSCLLVTLVLADHMLELRRDRDRARLLANVDGLTGALTRVAIERRLERDIDDARQ
CHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHH
MGTPLSVAFIDIDHFKRINDEYGHAAGDACLRQVVQRVRGWLRASDALGRHGGDELIAIL
CCCCEEEEEEEHHHHHHHCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
PGADLVAARRVAEGIRVAVSSQPFQFECTRLRCTLSLGVASWLPDESIEALLQRADNGLY
CCCHHHHHHHHHHHHEEEECCCCCEEEEEEEEEEEEHHHHHCCCCHHHHHHHHHHCCCCE
ASKAAGRDRVSTEM
ECCCCCCCCCCCCC
>Mature Secondary Structure
MKRQLGYFLLRGVLLWLCLAAPAVAQSLMLERLDDDPPAHEVLAGVYDAMLRPNDTGGAS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCH
IYETARHPVWWRIRADRQISAAGQPKLQIEFPYLNWVEAWVPGRSVPSHHAIYGAAADRR
HHHHCCCCEEEEEECCCEECCCCCCCEEEECCCHHHHHHHCCCCCCCCCCEEEECCCCCC
YATRALVIDLPEGLPQGRAVWLRVHAQSTISMPVSIVSNDVVHRRDLDHVAWYSFILGLT
HHEEEEEEECCCCCCCCCEEEEEEECCCCCCCCHHHHHCCHHHHCCCHHHHHHHHHHHHH
TVLAVLSASLWVRLREPVYGYFTVNLVCVLLYLIGFDGEARGFPVLGELFGSSPVPIRVV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCEEEE
ATFGGFVIVNFQRHYLNTAFRIPRVDLALKASSGWLLVMAVLNVVPQIQPYPVLTYLSLM
EECCCEEEEEEHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHH
VASLLLLFSCVWLGGVRGHRDAWSLLVAWTPLLLFSMLRSLELIGWNRLSGNGAWIGYGL
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEECC
SMSLGLSCLLVTLVLADHMLELRRDRDRARLLANVDGLTGALTRVAIERRLERDIDDARQ
CHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHH
MGTPLSVAFIDIDHFKRINDEYGHAAGDACLRQVVQRVRGWLRASDALGRHGGDELIAIL
CCCCEEEEEEEHHHHHHHCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
PGADLVAARRVAEGIRVAVSSQPFQFECTRLRCTLSLGVASWLPDESIEALLQRADNGLY
CCCHHHHHHHHHHHHEEEECCCCCEEEEEEEEEEEEHHHHHCCCCHHHHHHHHHHCCCCE
ASKAAGRDRVSTEM
ECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA