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The map label for this gene is gcvH

Identifier: 182680706

GI number: 182680706

Start: 190513

End: 190908

Strand: Reverse

Name: gcvH

Synonym: XfasM23_0135

Alternate gene names: 182680706

Gene position: 190908-190513 (Counterclockwise)

Preceding gene: 182680707

Following gene: 182680704

Centisome position: 7.53

GC content: 50.51

Gene sequence:

>396_bases
ATGAGTGATATCCCTGGCGACCTCAAGTTCCTCAAATCCCACGAATGGGTCCGGATCGAAGACAACAATCGTGCCATCGT
TGGCGTTTCCGACCATGCACAAAATCTGCTCGGTGATCTGGTGTATGTCGAATTACCCAATATTGGCGATCACCTGGACG
CTGGCGCCACCGCTGCAGTCATTGAGTCGGTCAAGGCAGCCTCAGATATCTACAGTCCAGTCACCGGCAAAGTGATTGAA
GTCAATACTACGTTAAGCGACAAACCTGAGACGATCAATGAAGACCCCTACGGTGAAGGCTGGATTATGGTCATTGAGAT
GCAGGCCCCCGAGGAGATCAGTAATCTACTTTCTCCCGATGATTACACCGAAGTGCTGGAAAGTGACGAACACTGA

Upstream 100 bases:

>100_bases
CAGATACACCCAGTGCTTACACGGCAGATAGTCTCTTGAAACAGTCTTATCCAATATCGGGTTGGTTAAACTATCTCCCC
CTTCCCGATGTACTTCTTCC

Downstream 100 bases:

>100_bases
ATCTTTCATCCATCCCCCACCCAATAGCCACCCACACACTGATCTATGGAGAGACGATCACAATCCATCGTAATCCTCAA
CAGAGAAGCATTAAACAAAA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 131; Mature: 130

Protein sequence:

>131_residues
MSDIPGDLKFLKSHEWVRIEDNNRAIVGVSDHAQNLLGDLVYVELPNIGDHLDAGATAAVIESVKAASDIYSPVTGKVIE
VNTTLSDKPETINEDPYGEGWIMVIEMQAPEEISNLLSPDDYTEVLESDEH

Sequences:

>Translated_131_residues
MSDIPGDLKFLKSHEWVRIEDNNRAIVGVSDHAQNLLGDLVYVELPNIGDHLDAGATAAVIESVKAASDIYSPVTGKVIE
VNTTLSDKPETINEDPYGEGWIMVIEMQAPEEISNLLSPDDYTEVLESDEH
>Mature_130_residues
SDIPGDLKFLKSHEWVRIEDNNRAIVGVSDHAQNLLGDLVYVELPNIGDHLDAGATAAVIESVKAASDIYSPVTGKVIEV
NTTLSDKPETINEDPYGEGWIMVIEMQAPEEISNLLSPDDYTEVLESDEH

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=123, Percent_Identity=39.8373983739837, Blast_Score=102, Evalue=8e-23,
Organism=Homo sapiens, GI89057342, Length=123, Percent_Identity=39.0243902439024, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1789271, Length=127, Percent_Identity=48.8188976377953, Blast_Score=134, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17551294, Length=121, Percent_Identity=39.6694214876033, Blast_Score=106, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17507493, Length=121, Percent_Identity=37.1900826446281, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319272, Length=132, Percent_Identity=37.1212121212121, Blast_Score=104, Evalue=5e-24,
Organism=Drosophila melanogaster, GI17865652, Length=128, Percent_Identity=39.0625, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_XYLF2 (B2I6Q0)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001828866.1
- ProteinModelPortal:   B2I6Q0
- SMR:   B2I6Q0
- GeneID:   6202290
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0135
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- ProtClustDB:   PRK01202
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14377; Mature: 14246

Theoretical pI: Translated: 3.86; Mature: 3.86

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIPGDLKFLKSHEWVRIEDNNRAIVGVSDHAQNLLGDLVYVELPNIGDHLDAGATAAV
CCCCCCCHHHHCCCCEEEEECCCEEEEEECHHHHHHHHCEEEEECCCCCCCCCCCHHHHH
IESVKAASDIYSPVTGKVIEVNTTLSDKPETINEDPYGEGWIMVIEMQAPEEISNLLSPD
HHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHCCCC
DYTEVLESDEH
HHHHHHCCCCC
>Mature Secondary Structure 
SDIPGDLKFLKSHEWVRIEDNNRAIVGVSDHAQNLLGDLVYVELPNIGDHLDAGATAAV
CCCCCCHHHHCCCCEEEEECCCEEEEEECHHHHHHHHCEEEEECCCCCCCCCCCHHHHH
IESVKAASDIYSPVTGKVIEVNTTLSDKPETINEDPYGEGWIMVIEMQAPEEISNLLSPD
HHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHCCCC
DYTEVLESDEH
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA