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The map label for this gene is lexA

Identifier: 182680656

GI number: 182680656

Start: 116852

End: 117487

Strand: Direct

Name: lexA

Synonym: XfasM23_0085

Alternate gene names: 182680656

Gene position: 116852-117487 (Clockwise)

Preceding gene: 182680655

Following gene: 182680657

Centisome position: 4.61

GC content: 52.52

Gene sequence:

>636_bases
ATGAGTTTGAGCGATATTCAGCAGGCAATCCTGTCATTGATTACCAACAACATCAACGCTGATGGCGTTTCTCCTTCGCA
GACGGAGATCGCGCGTGCATTTGGCTTCAAAGGGGTTCGCGCGGTGCAGCATCACCTTGATGTATTGGAGCAACAGGGGA
TGATTCGCCGCATCCCTGGACAGGCGCGTGGCATCCGGTTGAAGCATCTTACTGAGGTGGATGAGGTTGCGTTAGCTTTG
CAGAGTAAGGATGTGTTGCGCTTGCCAGTGCTCGGCCGCGTTGCGGCTGGTCAGCCGATCGGTGCTGATATCGGTGAGGA
TCACGTGGTGTTGTTGGATCGTGTGTTCTTCTCCCCAGCACCGGATTATCTGTTGAGGGTGCAAGGTGATTCGATGCGCG
ATGAAGGAATTTTCGATGGTGATTTGATCGGCGTACATCGTACGCAGGATGCGCATTCTGGGCAAATTGTGGTGGCGCGC
ATTGATGATGAGATTACCGTCAAATTGTTGAAGATCAGTAAAGACCGGATTCGTTTGCTACCGCGTAATCCTGACTTTGC
ACCGATTGAGGTGAGGTCAGATCAGGATTTCGCCATTGAGGGATTGTATTGCGGTTTGCTGCGCCCCAACCGGTGA

Upstream 100 bases:

>100_bases
TACTTGAATGCTGTGCTGGATCTGGCTGTATGGTCTTAAATGTTCCGCTTGCTAGGGAGGTTGCTTGTGTCTCTTGTACT
TATTAGTAATAATACTAATT

Downstream 100 bases:

>100_bases
CGCGATGTTCTTAATCCACGCGGTGTCTTTTTCTGAGGTTGTCACACGGGTGATGTCGTGATACCTGGCCTGCCTTGGCA
GTTTAAGCTGATGAGGCCAT

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MSLSDIQQAILSLITNNINADGVSPSQTEIARAFGFKGVRAVQHHLDVLEQQGMIRRIPGQARGIRLKHLTEVDEVALAL
QSKDVLRLPVLGRVAAGQPIGADIGEDHVVLLDRVFFSPAPDYLLRVQGDSMRDEGIFDGDLIGVHRTQDAHSGQIVVAR
IDDEITVKLLKISKDRIRLLPRNPDFAPIEVRSDQDFAIEGLYCGLLRPNR

Sequences:

>Translated_211_residues
MSLSDIQQAILSLITNNINADGVSPSQTEIARAFGFKGVRAVQHHLDVLEQQGMIRRIPGQARGIRLKHLTEVDEVALAL
QSKDVLRLPVLGRVAAGQPIGADIGEDHVVLLDRVFFSPAPDYLLRVQGDSMRDEGIFDGDLIGVHRTQDAHSGQIVVAR
IDDEITVKLLKISKDRIRLLPRNPDFAPIEVRSDQDFAIEGLYCGLLRPNR
>Mature_210_residues
SLSDIQQAILSLITNNINADGVSPSQTEIARAFGFKGVRAVQHHLDVLEQQGMIRRIPGQARGIRLKHLTEVDEVALALQ
SKDVLRLPVLGRVAAGQPIGADIGEDHVVLLDRVFFSPAPDYLLRVQGDSMRDEGIFDGDLIGVHRTQDAHSGQIVVARI
DDEITVKLLKISKDRIRLLPRNPDFAPIEVRSDQDFAIEGLYCGLLRPNR

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=208, Percent_Identity=44.2307692307692, Blast_Score=161, Evalue=3e-41,
Organism=Escherichia coli, GI1787431, Length=131, Percent_Identity=31.2977099236641, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_XYLF2 (B2I679)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001828816.1
- ProteinModelPortal:   B2I679
- SMR:   B2I679
- GeneID:   6202836
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0085
- HOGENOM:   HBG679610
- OMA:   ITNHINA
- ProtClustDB:   PRK00215
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 23323; Mature: 23192

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: ACT_SITE 131-131 ACT_SITE 168-168

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSDIQQAILSLITNNINADGVSPSQTEIARAFGFKGVRAVQHHLDVLEQQGMIRRIPG
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEECCC
QARGIRLKHLTEVDEVALALQSKDVLRLPVLGRVAAGQPIGADIGEDHVVLLDRVFFSPA
CCCCEEHHHHHHHHHHHHHHCCCCEEECCCCCHHCCCCCCCCCCCCCCEEEEEHHHCCCC
PDYLLRVQGDSMRDEGIFDGDLIGVHRTQDAHSGQIVVARIDDEITVKLLKISKDRIRLL
CCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCEEEEEEEECCCCEEEE
PRNPDFAPIEVRSDQDFAIEGLYCGLLRPNR
CCCCCCCEEEEECCCCEEEEEEEEEECCCCC
>Mature Secondary Structure 
SLSDIQQAILSLITNNINADGVSPSQTEIARAFGFKGVRAVQHHLDVLEQQGMIRRIPG
CHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEECCC
QARGIRLKHLTEVDEVALALQSKDVLRLPVLGRVAAGQPIGADIGEDHVVLLDRVFFSPA
CCCCEEHHHHHHHHHHHHHHCCCCEEECCCCCHHCCCCCCCCCCCCCCEEEEEHHHCCCC
PDYLLRVQGDSMRDEGIFDGDLIGVHRTQDAHSGQIVVARIDDEITVKLLKISKDRIRLL
CCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCEEEEEEEECCCCEEEE
PRNPDFAPIEVRSDQDFAIEGLYCGLLRPNR
CCCCCCCEEEEECCCCEEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA