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The map label for this gene is htrB [H]

Identifier: 182680641

GI number: 182680641

Start: 100110

End: 101030

Strand: Reverse

Name: htrB [H]

Synonym: XfasM23_0070

Alternate gene names: 182680641

Gene position: 101030-100110 (Counterclockwise)

Preceding gene: 182680654

Following gene: 182680637

Centisome position: 3.98

GC content: 56.13

Gene sequence:

>921_bases
ATGTCAGATTCTACAAACGTGACTCCCCGTCCATCCCTACTGAAACCAGCACACTGGCCAATGTATGTAGCACTGTGCGG
AATGGTCCTAGCCGCACGCCTGCCTTGGGGACTACAGCGCCGTCTGGGCCGGGTGATTGGAGCAATGGCGCTGCGCCTTG
TCGGGACGCGCCGGTGCGCCGCGGAGATTAATCTACAGCTGTGCTTTCCAACACACAATGAGGCGTGGCGGAAAAAGCTG
TTGCACGAGAATTTTGACGCCTTAGGCGTGGGCTTGTTCGAATTTGCACGCGCCTGGTGGGGAAGCATCGACGTGATCCG
TGCAGGCATACATGTGGAAGGGCTAGAACACCTACGCCAACTGCAACGCGAAGGCCGCGGCGTACTGCTTGTCTCCGGGC
ACTTCATGACATTAGAGATATGCGGACGGCTGCTCTGCGACTACGTGCCATTGGCCGGCATGTACCGGCGCCATCGCAAC
CCAGTATTGGAATGGGCGATCAAACGTGGACGGTTACGCTACGCCACCCAGATGTTTGCCAACGAGGAGTTGCGTGCAAC
GATCAAGCACCTGAAATGTGGTGGCTTCCTGTGGTATGCACCAGACCAAGACATGCGGGGCAAAGAGACCGTGTTTGCAC
CCTTCTTTGGGATGCCTGCAGCCACGATCACCGCAACGCATCAACTCGCCCGACTCACGGGCTGCGCGGTGGTACCTTAC
TTTCATCGCCGCCAAGGAGGGAACTATGTGTTGAAGATCGCACCACCGTTGGCAGATTTCCCATCCAAAGACCTCGCTGC
TGACACCGCACGCATTAATCAAGTAATTGAAACGATGATAGACGAGGCTCCGGATCAGTACTTATGGATTCATCGCCGTT
TTAAGCGACAACCAGAGGGACACAGCCGCTTCTACAACTAA

Upstream 100 bases:

>100_bases
ATTCAATCGGGTCTTTCCGCATGCATACCAGGATACGGAACCCGATGACCGCATGCATCTATCAGAGAACATACTCAAGC
CGATCCCATAGAATTACGCT

Downstream 100 bases:

>100_bases
GTGTATGGTGGTGATGGCCATGCTTGGAGCATGGATCAACATCTATTCCTTGCCGATCCCTTGAGCAGGCTGGCGCATCA
CCGATATTCACTACGACACG

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSDSTNVTPRPSLLKPAHWPMYVALCGMVLAARLPWGLQRRLGRVIGAMALRLVGTRRCAAEINLQLCFPTHNEAWRKKL
LHENFDALGVGLFEFARAWWGSIDVIRAGIHVEGLEHLRQLQREGRGVLLVSGHFMTLEICGRLLCDYVPLAGMYRRHRN
PVLEWAIKRGRLRYATQMFANEELRATIKHLKCGGFLWYAPDQDMRGKETVFAPFFGMPAATITATHQLARLTGCAVVPY
FHRRQGGNYVLKIAPPLADFPSKDLAADTARINQVIETMIDEAPDQYLWIHRRFKRQPEGHSRFYN

Sequences:

>Translated_306_residues
MSDSTNVTPRPSLLKPAHWPMYVALCGMVLAARLPWGLQRRLGRVIGAMALRLVGTRRCAAEINLQLCFPTHNEAWRKKL
LHENFDALGVGLFEFARAWWGSIDVIRAGIHVEGLEHLRQLQREGRGVLLVSGHFMTLEICGRLLCDYVPLAGMYRRHRN
PVLEWAIKRGRLRYATQMFANEELRATIKHLKCGGFLWYAPDQDMRGKETVFAPFFGMPAATITATHQLARLTGCAVVPY
FHRRQGGNYVLKIAPPLADFPSKDLAADTARINQVIETMIDEAPDQYLWIHRRFKRQPEGHSRFYN
>Mature_305_residues
SDSTNVTPRPSLLKPAHWPMYVALCGMVLAARLPWGLQRRLGRVIGAMALRLVGTRRCAAEINLQLCFPTHNEAWRKKLL
HENFDALGVGLFEFARAWWGSIDVIRAGIHVEGLEHLRQLQREGRGVLLVSGHFMTLEICGRLLCDYVPLAGMYRRHRNP
VLEWAIKRGRLRYATQMFANEELRATIKHLKCGGFLWYAPDQDMRGKETVFAPFFGMPAATITATHQLARLTGCAVVPYF
HRRQGGNYVLKIAPPLADFPSKDLAADTARINQVIETMIDEAPDQYLWIHRRFKRQPEGHSRFYN

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=297, Percent_Identity=39.3939393939394, Blast_Score=214, Evalue=8e-57,
Organism=Escherichia coli, GI87082094, Length=297, Percent_Identity=39.3939393939394, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1788161, Length=299, Percent_Identity=25.752508361204, Blast_Score=111, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 34958; Mature: 34827

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSTNVTPRPSLLKPAHWPMYVALCGMVLAARLPWGLQRRLGRVIGAMALRLVGTRRCA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
AEINLQLCFPTHNEAWRKKLLHENFDALGVGLFEFARAWWGSIDVIRAGIHVEGLEHLRQ
HEEEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHH
LQREGRGVLLVSGHFMTLEICGRLLCDYVPLAGMYRRHRNPVLEWAIKRGRLRYATQMFA
HHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHC
NEELRATIKHLKCGGFLWYAPDQDMRGKETVFAPFFGMPAATITATHQLARLTGCAVVPY
CHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCHHCCH
FHRRQGGNYVLKIAPPLADFPSKDLAADTARINQVIETMIDEAPDQYLWIHRRFKRQPEG
HHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
HSRFYN
CCCCCC
>Mature Secondary Structure 
SDSTNVTPRPSLLKPAHWPMYVALCGMVLAARLPWGLQRRLGRVIGAMALRLVGTRRCA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
AEINLQLCFPTHNEAWRKKLLHENFDALGVGLFEFARAWWGSIDVIRAGIHVEGLEHLRQ
HEEEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHH
LQREGRGVLLVSGHFMTLEICGRLLCDYVPLAGMYRRHRNPVLEWAIKRGRLRYATQMFA
HHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHC
NEELRATIKHLKCGGFLWYAPDQDMRGKETVFAPFFGMPAATITATHQLARLTGCAVVPY
CHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCHHCCH
FHRRQGGNYVLKIAPPLADFPSKDLAADTARINQVIETMIDEAPDQYLWIHRRFKRQPEG
HHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
HSRFYN
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]