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The map label for this gene is yjbJ [H]

Identifier: 170756547

GI number: 170756547

Start: 3541374

End: 3542069

Strand: Reverse

Name: yjbJ [H]

Synonym: CLD_1248

Alternate gene names: 170756547

Gene position: 3542069-3541374 (Counterclockwise)

Preceding gene: 170756222

Following gene: 170754583

Centisome position: 89.49

GC content: 31.61

Gene sequence:

>696_bases
ATGTTTAAAAATGGTGGTGGATTTATGAAAATAGATAATAATGAGATGAAATTACAACAAATGCTACAAATGCAATTGAT
GGGACAAATAATGAAGTCGTCCTTTGGAGATTCATCAAGCTTCCAAATAGTTTTAGATAGCTTATTAAAGGCTATGGAAA
ATACAGATAATAGTGATTTAAATAGTATATTATCATTTAATAGTGAAACTAACAGTGGGAATAAATATTATGGTGCAGGA
CAAAAATTAGAAGATGCAAAAAGAGAAATAAATAATATAAAATCTGAAGTTAGAACTGGTAATATAACTATAGATAGTGC
TGTAGAAAAAGCTTCAAGAAAATATGGTATAGACAAAGAACTTCTAATGGCAGTTATAAAGCAAGAATCGGATTTCAATC
CTAATTGCGTATCAAGTGCAGGGGCAAAGGGGTTAATGCAGCTTATGCCAGGTACTGCTAGAGAAGTAGGAGTTACAAAT
CCCTTCGATATAGAACAAAATATAGATGGGGGAACAAAATATTTAAAGAAAATGTTAGATATGCATGGAAACGTAAAAGA
GTTAGCTTTGGCAGCATATAACGCAGGTCCTGGAACCCTCCAGTGGAGAGGTGTAAAAAGCCCTTCAGATATAAATAGAT
TGCCAAGTGAAACAAGAAATTATGTAAAAAATATAATGAAAAATTATGGAGTATAA

Upstream 100 bases:

>100_bases
TTATCCAAAAGTAGCTTTTTAAAGTGTGCTTATGAAGATTATGACATATTTAAATTTAAAGTGTAAATAAAGCAGAAATC
CAAACGATAATATTAGATAT

Downstream 100 bases:

>100_bases
AAAGGAAAGTGCCCTGCACTTTCCTTTAAATTTGTTATAATGGTAATACTAATAATAGAATAATCAAAACTAGGAGGAAT
TTCATGGAACGTTTAGATAA

Product: Slt family transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MFKNGGGFMKIDNNEMKLQQMLQMQLMGQIMKSSFGDSSSFQIVLDSLLKAMENTDNSDLNSILSFNSETNSGNKYYGAG
QKLEDAKREINNIKSEVRTGNITIDSAVEKASRKYGIDKELLMAVIKQESDFNPNCVSSAGAKGLMQLMPGTAREVGVTN
PFDIEQNIDGGTKYLKKMLDMHGNVKELALAAYNAGPGTLQWRGVKSPSDINRLPSETRNYVKNIMKNYGV

Sequences:

>Translated_231_residues
MFKNGGGFMKIDNNEMKLQQMLQMQLMGQIMKSSFGDSSSFQIVLDSLLKAMENTDNSDLNSILSFNSETNSGNKYYGAG
QKLEDAKREINNIKSEVRTGNITIDSAVEKASRKYGIDKELLMAVIKQESDFNPNCVSSAGAKGLMQLMPGTAREVGVTN
PFDIEQNIDGGTKYLKKMLDMHGNVKELALAAYNAGPGTLQWRGVKSPSDINRLPSETRNYVKNIMKNYGV
>Mature_231_residues
MFKNGGGFMKIDNNEMKLQQMLQMQLMGQIMKSSFGDSSSFQIVLDSLLKAMENTDNSDLNSILSFNSETNSGNKYYGAG
QKLEDAKREINNIKSEVRTGNITIDSAVEKASRKYGIDKELLMAVIKQESDFNPNCVSSAGAKGLMQLMPGTAREVGVTN
PFDIEQNIDGGTKYLKKMLDMHGNVKELALAAYNAGPGTLQWRGVKSPSDINRLPSETRNYVKNIMKNYGV

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=127, Percent_Identity=40.1574803149606, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI87082191, Length=111, Percent_Identity=36.9369369369369, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI171474010, Length=159, Percent_Identity=31.4465408805031, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 25514; Mature: 25514

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKNGGGFMKIDNNEMKLQQMLQMQLMGQIMKSSFGDSSSFQIVLDSLLKAMENTDNSDL
CCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHH
NSILSFNSETNSGNKYYGAGQKLEDAKREINNIKSEVRTGNITIDSAVEKASRKYGIDKE
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCHH
LLMAVIKQESDFNPNCVSSAGAKGLMQLMPGTAREVGVTNPFDIEQNIDGGTKYLKKMLD
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHH
MHGNVKELALAAYNAGPGTLQWRGVKSPSDINRLPSETRNYVKNIMKNYGV
HCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFKNGGGFMKIDNNEMKLQQMLQMQLMGQIMKSSFGDSSSFQIVLDSLLKAMENTDNSDL
CCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHH
NSILSFNSETNSGNKYYGAGQKLEDAKREINNIKSEVRTGNITIDSAVEKASRKYGIDKE
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCHH
LLMAVIKQESDFNPNCVSSAGAKGLMQLMPGTAREVGVTNPFDIEQNIDGGTKYLKKMLD
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHH
MHGNVKELALAAYNAGPGTLQWRGVKSPSDINRLPSETRNYVKNIMKNYGV
HCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]