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The map label for this gene is nadB

Identifier: 170756082

GI number: 170756082

Start: 1611215

End: 1612516

Strand: Reverse

Name: nadB

Synonym: CLD_3102

Alternate gene names: 170756082

Gene position: 1612516-1611215 (Counterclockwise)

Preceding gene: 170755261

Following gene: 170757583

Centisome position: 40.74

GC content: 27.96

Gene sequence:

>1302_bases
ATGAATCTTTATGCTGATGTCTTGATTGCAGGAAGCGGCGTAGCTGGATTATATTCAGCTTTAAATCTAAGAAAAGACTT
AAAAATAGTATTAATCTCAAAAGGTAAACTTAACGAATGTAATACTACCCTAGCCCAGGGTGGAATCTCTGTAGCAAGGA
ATAAGGATGATATAACCACTTTTGTAGAAGATACTTTAAAAGCAGGATCCTATGAAAATAACTTGTGTGCAGTAAAAATA
TTAGCAAATGAATCTATAGAAAACATTAATAAATTACAGGATATGGGCCTTAACTTGGATAAATGTAAAAAGGAATTAAG
CTATACAAAAGAAGGAGCTCATAGTATAAACAGAATTGTTCATTTTAAGGATTATACTGGTAAAAAATTAGAAGAAATCT
TAACTAAGAAAATACTTAAAAGAAAGAATATAACTGTAATTGAAAATTGTAGCCTTGTAGATTTAATACAAAATAATAAT
ACTTGCTTTGGAGGTATATGTTTAAAAGATAATAAGCAAATAAACATATATTCAAAAGTAACAATACTGGCAACAGGAGG
TATAGGAGGATTATTTAAAAACTCAACTAATGAACCAATTATAACTGGAGATGGTATATCTATAGCTATAAAAAACAATA
TAAAAATTAAAAACTTGGATTATATACAATTTCATCCTACAGCATTTTTTAAGAATACTATTAAGGGAAAAAAATTTTTA
ATTTCTGAGTCAGTACGAGGAGAAGGAGGAAAGCTTTTAAATTCTAAAGGTGAAAGATTTGTAAATGAATTACTCTCTAG
AGATATAGTAACCAAGTATATCCTAGAAGAGGAAAGAAAAACCGGCTCTAATAATGTATATTTAGATATCACCTTTAAAC
CAGGAACTTTTTTAAAGAAAAGATTCCCTACTATTTATGAAAATTGTTTAAAAAATGGCGTAAATATTTGTAAAGATGCT
ATTCCTGTATCTCCGGCACAACATTATTTCATGGGTGGAATACAAGTAGACTTATTTGGACAATCTTCTATGAATCATTT
ATATGCTTTTGGTGAAGTAAGTTGCACTGGAGTACACGGTAAAAATAGACTTGCTAGCAACTCCCTACTAGAAGGACTAG
TATTTTCAAAACGTGGCGCTGAAAAAATAAATTTTGAAATAAATAATATTAAATTATCATATACAAAGATTTATACATTG
AAACATAATGTAGAATATTATAGTTTATTAAATAAAAAAATAATTACAAAATCTCTACTTAAACTAAGGAGTGATTTACA
AAATGAATTGGTTAGTTGTTGA

Upstream 100 bases:

>100_bases
GTCTTTTAAATTTAAATTATAAAATAGAATTAGATAAAAATTTAAGATTAAAGGCCTATGATGCCCTTATAAATATGCAT
ACTTTAGGAGGCAGATAACC

Downstream 100 bases:

>100_bases
TGAAATACTTAAATCTGCACTTAAAGAAGATATATCCTTTGAGGACATAACTACAGAATCTATATTTAAAGAAAATAAAA
GAGCAAAAATAGATTTAATT

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MNLYADVLIAGSGVAGLYSALNLRKDLKIVLISKGKLNECNTTLAQGGISVARNKDDITTFVEDTLKAGSYENNLCAVKI
LANESIENINKLQDMGLNLDKCKKELSYTKEGAHSINRIVHFKDYTGKKLEEILTKKILKRKNITVIENCSLVDLIQNNN
TCFGGICLKDNKQINIYSKVTILATGGIGGLFKNSTNEPIITGDGISIAIKNNIKIKNLDYIQFHPTAFFKNTIKGKKFL
ISESVRGEGGKLLNSKGERFVNELLSRDIVTKYILEEERKTGSNNVYLDITFKPGTFLKKRFPTIYENCLKNGVNICKDA
IPVSPAQHYFMGGIQVDLFGQSSMNHLYAFGEVSCTGVHGKNRLASNSLLEGLVFSKRGAEKINFEINNIKLSYTKIYTL
KHNVEYYSLLNKKIITKSLLKLRSDLQNELVSC

Sequences:

>Translated_433_residues
MNLYADVLIAGSGVAGLYSALNLRKDLKIVLISKGKLNECNTTLAQGGISVARNKDDITTFVEDTLKAGSYENNLCAVKI
LANESIENINKLQDMGLNLDKCKKELSYTKEGAHSINRIVHFKDYTGKKLEEILTKKILKRKNITVIENCSLVDLIQNNN
TCFGGICLKDNKQINIYSKVTILATGGIGGLFKNSTNEPIITGDGISIAIKNNIKIKNLDYIQFHPTAFFKNTIKGKKFL
ISESVRGEGGKLLNSKGERFVNELLSRDIVTKYILEEERKTGSNNVYLDITFKPGTFLKKRFPTIYENCLKNGVNICKDA
IPVSPAQHYFMGGIQVDLFGQSSMNHLYAFGEVSCTGVHGKNRLASNSLLEGLVFSKRGAEKINFEINNIKLSYTKIYTL
KHNVEYYSLLNKKIITKSLLKLRSDLQNELVSC
>Mature_433_residues
MNLYADVLIAGSGVAGLYSALNLRKDLKIVLISKGKLNECNTTLAQGGISVARNKDDITTFVEDTLKAGSYENNLCAVKI
LANESIENINKLQDMGLNLDKCKKELSYTKEGAHSINRIVHFKDYTGKKLEEILTKKILKRKNITVIENCSLVDLIQNNN
TCFGGICLKDNKQINIYSKVTILATGGIGGLFKNSTNEPIITGDGISIAIKNNIKIKNLDYIQFHPTAFFKNTIKGKKFL
ISESVRGEGGKLLNSKGERFVNELLSRDIVTKYILEEERKTGSNNVYLDITFKPGTFLKKRFPTIYENCLKNGVNICKDA
IPVSPAQHYFMGGIQVDLFGQSSMNHLYAFGEVSCTGVHGKNRLASNSLLEGLVFSKRGAEKINFEINNIKLSYTKIYTL
KHNVEYYSLLNKKIITKSLLKLRSDLQNELVSC

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=380, Percent_Identity=31.8421052631579, Blast_Score=176, Evalue=5e-44,
Organism=Escherichia coli, GI1788928, Length=399, Percent_Identity=39.5989974937343, Blast_Score=284, Evalue=9e-78,
Organism=Escherichia coli, GI1786942, Length=386, Percent_Identity=31.6062176165803, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1790597, Length=384, Percent_Identity=28.6458333333333, Blast_Score=162, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17550100, Length=388, Percent_Identity=31.9587628865979, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17505833, Length=388, Percent_Identity=31.1855670103093, Blast_Score=167, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI71986328, Length=421, Percent_Identity=31.5914489311164, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6322701, Length=395, Percent_Identity=31.1392405063291, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322416, Length=394, Percent_Identity=30.4568527918782, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6322511, Length=306, Percent_Identity=28.7581699346405, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6320788, Length=312, Percent_Identity=28.2051282051282, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17137288, Length=449, Percent_Identity=29.8440979955457, Blast_Score=168, Evalue=8e-42,
Organism=Drosophila melanogaster, GI24655642, Length=449, Percent_Identity=29.8440979955457, Blast_Score=168, Evalue=8e-42,
Organism=Drosophila melanogaster, GI24655647, Length=449, Percent_Identity=29.8440979955457, Blast_Score=168, Evalue=8e-42,
Organism=Drosophila melanogaster, GI24663005, Length=393, Percent_Identity=29.0076335877863, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027 [H]

Pfam domain/function: PF00890 FAD_binding_2 [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 48265; Mature: 48265

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLYADVLIAGSGVAGLYSALNLRKDLKIVLISKGKLNECNTTLAQGGISVARNKDDITT
CCEEEEEEEECCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCEEECCCHHHHH
FVEDTLKAGSYENNLCAVKILANESIENINKLQDMGLNLDKCKKELSYTKEGAHSINRIV
HHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEE
HFKDYTGKKLEEILTKKILKRKNITVIENCSLVDLIQNNNTCFGGICLKDNKQINIYSKV
EECCCCCHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCEEEEEEEECCCCEEEEEEEE
TILATGGIGGLFKNSTNEPIITGDGISIAIKNNIKIKNLDYIQFHPTAFFKNTIKGKKFL
EEEEECCCCCCCCCCCCCCEEECCCEEEEEECCEEEECCEEEEECCHHHHHHCCCCCEEE
ISESVRGEGGKLLNSKGERFVNELLSRDIVTKYILEEERKTGSNNVYLDITFKPGTFLKK
EECCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHH
RFPTIYENCLKNGVNICKDAIPVSPAQHYFMGGIQVDLFGQSSMNHLYAFGEVSCTGVHG
HCHHHHHHHHHCCHHHHHHCCCCCCCHHHEECCEEEEEECCCCCCCEEEECCEEEEECCC
KNRLASNSLLEGLVFSKRGAEKINFEINNIKLSYTKIYTLKHNVEYYSLLNKKIITKSLL
CCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHH
KLRSDLQNELVSC
HHHHHHHHHHHCC
>Mature Secondary Structure
MNLYADVLIAGSGVAGLYSALNLRKDLKIVLISKGKLNECNTTLAQGGISVARNKDDITT
CCEEEEEEEECCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCEEECCCHHHHH
FVEDTLKAGSYENNLCAVKILANESIENINKLQDMGLNLDKCKKELSYTKEGAHSINRIV
HHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEE
HFKDYTGKKLEEILTKKILKRKNITVIENCSLVDLIQNNNTCFGGICLKDNKQINIYSKV
EECCCCCHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCEEEEEEEECCCCEEEEEEEE
TILATGGIGGLFKNSTNEPIITGDGISIAIKNNIKIKNLDYIQFHPTAFFKNTIKGKKFL
EEEEECCCCCCCCCCCCCCEEECCCEEEEEECCEEEECCEEEEECCHHHHHHCCCCCEEE
ISESVRGEGGKLLNSKGERFVNELLSRDIVTKYILEEERKTGSNNVYLDITFKPGTFLKK
EECCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHH
RFPTIYENCLKNGVNICKDAIPVSPAQHYFMGGIQVDLFGQSSMNHLYAFGEVSCTGVHG
HCHHHHHHHHHCCHHHHHHCCCCCCCHHHEECCEEEEEECCCCCCCEEEECCEEEEECCC
KNRLASNSLLEGLVFSKRGAEKINFEINNIKLSYTKIYTLKHNVEYYSLLNKKIITKSLL
CCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHH
KLRSDLQNELVSC
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]