| Definition | Clostridium botulinum B1 str. Okra, complete genome. |
|---|---|
| Accession | NC_010516 |
| Length | 3,958,233 |
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The map label for this gene is ptsG
Identifier: 170755505
GI number: 170755505
Start: 1470182
End: 1472212
Strand: Reverse
Name: ptsG
Synonym: CLD_3231
Alternate gene names: 170755505
Gene position: 1472212-1470182 (Counterclockwise)
Preceding gene: 170756424
Following gene: 170754241
Centisome position: 37.19
GC content: 32.94
Gene sequence:
>2031_bases ATGTTTAAGAAATCATTCGGTGTTTTACAACAAGTTGGTAAAGCTTTAATGCTTCCTGTAGCATTATTACCTGCCGCTGG TATTTTATTAGCCTTTGGTAATATGTTTCAAAACCCTGACTTTTTAAAACTTGCTCCAGCTTTTGGTTCAACAGGATTTC AAGCTTTCGCAAGAGTTATGGAACAATCAGGTAATATAATTTTTGCTAATCTAGCCTTATTATTTGCAGTAGGTGTTGCT GTAGGATTAGCAGCAGGTGAAGGTGTTGCCGCACTAGCAGCTATAGTTGGATTCTTAATTATGAATACTACTATGGGTCT TGTAGTTGGTGTAACTCCAGGATTAATAGGTAGATCTCATCCTGAGTTTGCAAGTGTTTTAGGTATACCAACACTACAAA CCGGGGTATTTGGCGGTATTATAATGGGTATTATTGCAGCATCATTATATAAGAAATATTACAATATAGAATTACCTTCT TATTTAGGATTCTTCGCAGGTAAAAGATTTGTTCCAATAATAACTGCAGCAGTTGCTATTATTGTTGGTTTAATAATGGT AGTTATTTGGCCTCCAATCCAACATGGATTAAATTCATTCTCACACAACATGATAGATGCTAATAGAACTTTAGCAGCTT TTATATTTGGTGTTATTGAAAGAGCATTAATTCCATTTGGGTTACATCATATATTCTATAATCCATTCTGGTATCAATTT GGTGAATATATAAACAAAGCAGGTCAAGTTATAAACGGAGATCAAGCTATATTCTTCCAACAAATTAAAGATCATGCGCC TCTTACAGCAGGTACATTTATGACTGGTAAATTTCCATTTATGATGTTTGGTCTTCCAGCTGCTGCTTTAGCTATATATC AGGAAGCTAAACCTGAAAAGAAAAAACTCGTTGGTGGTATAATGGCCTCTGCAGCTTTAACTTCATTCTTAACAGGTATA ACAGAACCAATTGAATTCTCATTCTTATTCGTTGCACCAGTATTATTCGGAATTCACTGTATATTTGCAGGATTATCTTT CATGGTTATGCAAATATTAAATATCAAAATTGGTATGACATTCTCTGGTGGTGTTATAGATTTCTTAATATTTGGTGTTG TTCCAAATAGAACTAAATGGTGGCTTGTAATTCCTGTAGGCTTAGCTTTCTCTGTAATTTACTATTTTGGATTTAGATTT GCTATAAGGAAATGGAACCTAAAAACTCCTGGTCGTGAAGATGATGCAGGTTCAGAAAGTGTTAACTACAGTTCTGAAGG CTCTTTAGCTGCTAAAGTATTAGAAGCTTTAGGTGGAAAAGAAAATTTAGCTAACTTAGATGCTTGTATAACTCGTCTTA GAGTAAGTGTTAATAGTATTGATAAAGTAAATAAAGAAGAACTAAAATCATTAGGTGCTTCTGGAGTTATGGTAGTTGGT AATAATATACAAGCTATCTTTGGGCCAAAATCTGATCAGTTAAAAGAGCAAATTAAAGATGTTATATCTGGAAAAATTAT AACTAAAGATGTTAAAATAGAAGAACCTAAAAAAGAAACTGTAAAAGTTTCTTCTAAAGATAAATTTGTAGCCCCAATAG AAGGAAAAATACTTTCTATAACTGATGTTCCAGATGAAGTTTTCTCTCAAAAAATGATGGGTGACGGATTTGCTATAGAG CCTAAAAATGGAACAGTAGTTTCCCCTGTAGATGGAGTAATAACTACAGTATTCCCAACTAAGCATGCTATAGGAATAAC AGCAGAAAACGGACTTGAGCTTTTGATCCACTTTGGAATAGATACAGTTAATCTTAAAGGCGAAGGCTTAGAAGCCTTAG TAGAACAAGATGCTAAAGTTAAAGCAGGAGATCCAATATTAAAAGTAGATATTGATAAAATTAAGGATGAAGTTCCTTCA ATAATAACTCCAATAATATTTACTAATTTAACTGATGATCAAAAATTAGAAATTGTTAAACTTGGTAAAACAGTAAAAGC TGGAGAAGAAGATATAGTTAACTTGAAATAA
Upstream 100 bases:
>100_bases TTTTAATCTTATTTAAAGTTTTACTCATGCCTTTTGTATTTTAGTAAACAATATTATTAAAAAATAAATTTATTGTATGA TATGAAAGGAGAACTTATTT
Downstream 100 bases:
>100_bases AAAATATACTTTAAAATAGGAACTGTTTTAAAATAAAAATAGCTTTAATTTTAATATAGTAAATATAGAAAATATATGTA ATAAGCATATGAATAAGCTT
Product: PTS system, glucose-specific, IIABC component
Products: NA
Alternate protein names: EII-Glc/EIII-Glc; EIICBA-Glc; Glucose permease IIC component; PTS system glucose-specific EIIC component; Glucose-specific phosphotransferase enzyme IIB component; PTS system glucose-specific EIIB component; Glucose-specific phosphotransferase enzyme IIA component; PTS system glucose-specific EIIA component [H]
Number of amino acids: Translated: 676; Mature: 676
Protein sequence:
>676_residues MFKKSFGVLQQVGKALMLPVALLPAAGILLAFGNMFQNPDFLKLAPAFGSTGFQAFARVMEQSGNIIFANLALLFAVGVA VGLAAGEGVAALAAIVGFLIMNTTMGLVVGVTPGLIGRSHPEFASVLGIPTLQTGVFGGIIMGIIAASLYKKYYNIELPS YLGFFAGKRFVPIITAAVAIIVGLIMVVIWPPIQHGLNSFSHNMIDANRTLAAFIFGVIERALIPFGLHHIFYNPFWYQF GEYINKAGQVINGDQAIFFQQIKDHAPLTAGTFMTGKFPFMMFGLPAAALAIYQEAKPEKKKLVGGIMASAALTSFLTGI TEPIEFSFLFVAPVLFGIHCIFAGLSFMVMQILNIKIGMTFSGGVIDFLIFGVVPNRTKWWLVIPVGLAFSVIYYFGFRF AIRKWNLKTPGREDDAGSESVNYSSEGSLAAKVLEALGGKENLANLDACITRLRVSVNSIDKVNKEELKSLGASGVMVVG NNIQAIFGPKSDQLKEQIKDVISGKIITKDVKIEEPKKETVKVSSKDKFVAPIEGKILSITDVPDEVFSQKMMGDGFAIE PKNGTVVSPVDGVITTVFPTKHAIGITAENGLELLIHFGIDTVNLKGEGLEALVEQDAKVKAGDPILKVDIDKIKDEVPS IITPIIFTNLTDDQKLEIVKLGKTVKAGEEDIVNLK
Sequences:
>Translated_676_residues MFKKSFGVLQQVGKALMLPVALLPAAGILLAFGNMFQNPDFLKLAPAFGSTGFQAFARVMEQSGNIIFANLALLFAVGVA VGLAAGEGVAALAAIVGFLIMNTTMGLVVGVTPGLIGRSHPEFASVLGIPTLQTGVFGGIIMGIIAASLYKKYYNIELPS YLGFFAGKRFVPIITAAVAIIVGLIMVVIWPPIQHGLNSFSHNMIDANRTLAAFIFGVIERALIPFGLHHIFYNPFWYQF GEYINKAGQVINGDQAIFFQQIKDHAPLTAGTFMTGKFPFMMFGLPAAALAIYQEAKPEKKKLVGGIMASAALTSFLTGI TEPIEFSFLFVAPVLFGIHCIFAGLSFMVMQILNIKIGMTFSGGVIDFLIFGVVPNRTKWWLVIPVGLAFSVIYYFGFRF AIRKWNLKTPGREDDAGSESVNYSSEGSLAAKVLEALGGKENLANLDACITRLRVSVNSIDKVNKEELKSLGASGVMVVG NNIQAIFGPKSDQLKEQIKDVISGKIITKDVKIEEPKKETVKVSSKDKFVAPIEGKILSITDVPDEVFSQKMMGDGFAIE PKNGTVVSPVDGVITTVFPTKHAIGITAENGLELLIHFGIDTVNLKGEGLEALVEQDAKVKAGDPILKVDIDKIKDEVPS IITPIIFTNLTDDQKLEIVKLGKTVKAGEEDIVNLK >Mature_676_residues MFKKSFGVLQQVGKALMLPVALLPAAGILLAFGNMFQNPDFLKLAPAFGSTGFQAFARVMEQSGNIIFANLALLFAVGVA VGLAAGEGVAALAAIVGFLIMNTTMGLVVGVTPGLIGRSHPEFASVLGIPTLQTGVFGGIIMGIIAASLYKKYYNIELPS YLGFFAGKRFVPIITAAVAIIVGLIMVVIWPPIQHGLNSFSHNMIDANRTLAAFIFGVIERALIPFGLHHIFYNPFWYQF GEYINKAGQVINGDQAIFFQQIKDHAPLTAGTFMTGKFPFMMFGLPAAALAIYQEAKPEKKKLVGGIMASAALTSFLTGI TEPIEFSFLFVAPVLFGIHCIFAGLSFMVMQILNIKIGMTFSGGVIDFLIFGVVPNRTKWWLVIPVGLAFSVIYYFGFRF AIRKWNLKTPGREDDAGSESVNYSSEGSLAAKVLEALGGKENLANLDACITRLRVSVNSIDKVNKEELKSLGASGVMVVG NNIQAIFGPKSDQLKEQIKDVISGKIITKDVKIEEPKKETVKVSSKDKFVAPIEGKILSITDVPDEVFSQKMMGDGFAIE PKNGTVVSPVDGVITTVFPTKHAIGITAENGLELLIHFGIDTVNLKGEGLEALVEQDAKVKAGDPILKVDIDKIKDEVPS IITPIIFTNLTDDQKLEIVKLGKTVKAGEEDIVNLK
Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This
COG id: COG1263
COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific
Gene ontology:
Cell location: Cell membrane; Multi-pass membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 PTS EIIC type-1 domain [H]
Homologues:
Organism=Escherichia coli, GI1787343, Length=502, Percent_Identity=49.402390438247, Blast_Score=437, Evalue=1e-123, Organism=Escherichia coli, GI1786894, Length=688, Percent_Identity=37.3546511627907, Blast_Score=424, Evalue=1e-119, Organism=Escherichia coli, GI1787908, Length=516, Percent_Identity=37.984496124031, Blast_Score=327, Evalue=2e-90, Organism=Escherichia coli, GI1788757, Length=149, Percent_Identity=41.6107382550336, Blast_Score=132, Evalue=1e-31, Organism=Escherichia coli, GI1790159, Length=140, Percent_Identity=37.8571428571429, Blast_Score=100, Evalue=3e-22,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR011055 - InterPro: IPR018113 - InterPro: IPR004719 - InterPro: IPR001127 - InterPro: IPR001996 - InterPro: IPR003352 - InterPro: IPR013013 - InterPro: IPR011535 - InterPro: IPR011299 [H]
Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]
EC number: =2.7.1.69 [H]
Molecular weight: Translated: 72863; Mature: 72863
Theoretical pI: Translated: 8.19; Mature: 8.19
Prosite motif: PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1 ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.3 %Cys (Translated Protein) 2.8 %Met (Translated Protein) 3.1 %Cys+Met (Translated Protein) 0.3 %Cys (Mature Protein) 2.8 %Met (Mature Protein) 3.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MFKKSFGVLQQVGKALMLPVALLPAAGILLAFGNMFQNPDFLKLAPAFGSTGFQAFARVM CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHH EQSGNIIFANLALLFAVGVAVGLAAGEGVAALAAIVGFLIMNTTMGLVVGVTPGLIGRSH HHCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCC PEFASVLGIPTLQTGVFGGIIMGIIAASLYKKYYNIELPSYLGFFAGKRFVPIITAAVAI CHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHH IVGLIMVVIWPPIQHGLNSFSHNMIDANRTLAAFIFGVIERALIPFGLHHIFYNPFWYQF HHHHHHHHHCCCHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH GEYINKAGQVINGDQAIFFQQIKDHAPLTAGTFMTGKFPFMMFGLPAAALAIYQEAKPEK HHHHHHCCCEECCCHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCHH KKLVGGIMASAALTSFLTGITEPIEFSFLFVAPVLFGIHCIFAGLSFMVMQILNIKIGMT HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE FSGGVIDFLIFGVVPNRTKWWLVIPVGLAFSVIYYFGFRFAIRKWNLKTPGREDDAGSES ECCCHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCC VNYSSEGSLAAKVLEALGGKENLANLDACITRLRVSVNSIDKVNKEELKSLGASGVMVVG CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEC NNIQAIFGPKSDQLKEQIKDVISGKIITKDVKIEEPKKETVKVSSKDKFVAPIEGKILSI CCEEEEECCCHHHHHHHHHHHHCCCEEEECCCCCCCCCHHEEECCCCCEEECCCCCEEEE TDVPDEVFSQKMMGDGFAIEPKNGTVVSPVDGVITTVFPTKHAIGITAENGLELLIHFGI ECCCHHHHHHHHCCCCEEEECCCCCEECCCCCCEEEECCCCCEEEEEECCCCEEEEEECC DTVNLKGEGLEALVEQDAKVKAGDPILKVDIDKIKDEVPSIITPIIFTNLTDDQKLEIVK EEEEECCCCHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE LGKTVKAGEEDIVNLK ECCCCCCCCCCCCCCC >Mature Secondary Structure MFKKSFGVLQQVGKALMLPVALLPAAGILLAFGNMFQNPDFLKLAPAFGSTGFQAFARVM CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHH EQSGNIIFANLALLFAVGVAVGLAAGEGVAALAAIVGFLIMNTTMGLVVGVTPGLIGRSH HHCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCC PEFASVLGIPTLQTGVFGGIIMGIIAASLYKKYYNIELPSYLGFFAGKRFVPIITAAVAI CHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHH IVGLIMVVIWPPIQHGLNSFSHNMIDANRTLAAFIFGVIERALIPFGLHHIFYNPFWYQF HHHHHHHHHCCCHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH GEYINKAGQVINGDQAIFFQQIKDHAPLTAGTFMTGKFPFMMFGLPAAALAIYQEAKPEK HHHHHHCCCEECCCHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCHH KKLVGGIMASAALTSFLTGITEPIEFSFLFVAPVLFGIHCIFAGLSFMVMQILNIKIGMT HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE FSGGVIDFLIFGVVPNRTKWWLVIPVGLAFSVIYYFGFRFAIRKWNLKTPGREDDAGSES ECCCHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCC VNYSSEGSLAAKVLEALGGKENLANLDACITRLRVSVNSIDKVNKEELKSLGASGVMVVG CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEC NNIQAIFGPKSDQLKEQIKDVISGKIITKDVKIEEPKKETVKVSSKDKFVAPIEGKILSI CCEEEEECCCHHHHHHHHHHHHCCCEEEECCCCCCCCCHHEEECCCCCEEECCCCCEEEE TDVPDEVFSQKMMGDGFAIEPKNGTVVSPVDGVITTVFPTKHAIGITAENGLELLIHFGI ECCCHHHHHHHHCCCCEEEECCCCCEECCCCCCEEEECCCCCEEEEEECCCCEEEEEECC DTVNLKGEGLEALVEQDAKVKAGDPILKVDIDKIKDEVPSIITPIIFTNLTDDQKLEIVK EEEEECCCCHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE LGKTVKAGEEDIVNLK ECCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 1508157; 9384377; 2497294; 2120236; 1911744; 1906345; 9593197 [H]